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OPB
Wanderer

Registered: 05/30/15
Posts: 198
Last seen: 2 years, 10 days
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Bunk syringe - reinoculate?
#21908887 - 07/07/15 09:39 AM (8 years, 6 months ago) |
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I have 3 quarts of rye that I inoculated a a week and a half ago. 2 jars I inoculated with a syringe I purchased. 1 jar I inoculated with a syringe I made from a spore print. The 1 I made is showing colonization down the entire side by the port and the bottom. The other 2 don't show the slightest hint of fuzz. I know agar is the preferred method and I am working on that, but these three jars were for a monotub and I don't want to try to pull it off with only one quart of spawn. Can I reinoculate the other two jars with one of my syringes? I know this increases the chance of contamination but they seem dead in the water as it is.
Thanks OPB
-------------------- "Wasn't that fun"
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Grim767
Traveler of the Abyss



Registered: 05/30/15
Posts: 225
Loc: The Abyss
Last seen: 7 years, 3 months
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Re: Bunk syringe - reinoculate? [Re: OPB]
#21908949 - 07/07/15 09:51 AM (8 years, 6 months ago) |
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I had that same problem a bunk syringe from shitty supplier after 3 weeks no growth I re inoculated with syringe from a sponsor and week later growth in all three jars
-------------------- Trade List I won't bow to something that I've never seen, Can't believe in something that doesn't believe in me, I'm not blood of your blood, I'm no son of your god, I have no faith in your faith, Still I find salvation.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Bunk syringe - reinoculate? [Re: Grim767]
#21909020 - 07/07/15 10:11 AM (8 years, 6 months ago) |
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http://www.shroomery.org/forums/showflat.php/Number/15383615
I've had stubborn syringes that I did this on and they germinated 3 days later.
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OPB
Wanderer

Registered: 05/30/15
Posts: 198
Last seen: 2 years, 10 days
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Re: Bunk syringe - reinoculate? [Re: Mad Season]
#21909041 - 07/07/15 10:16 AM (8 years, 6 months ago) |
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Quote:
Grim767 said: I had that same problem a bunk syringe from shitty supplier after 3 weeks no growth I re inoculated with syringe from a sponsor and week later growth in all three jars
Great! Thanks
Quote:
Mad Season said: http://www.shroomery.org/forums/showflat.php/Number/15383615
I've had stubborn syringes that I did this on and they germinated 3 days later.
That's super interesting. I will have to keep that in mind for the future! I am thinking though that I will just use my spore syringe this time because I've found most of this suppliers syringes to be hugely dissapointing. I am more than happy to squirt them down the drain.
-------------------- "Wasn't that fun"
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oontribe

Registered: 01/14/15
Posts: 3,570
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Re: Bunk syringe - reinoculate? [Re: OPB]
#21909511 - 07/07/15 12:26 PM (8 years, 6 months ago) |
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Iam facing the same problem...couldnt scrap prints and turn em into a syringe zo most probably i inoculated sterile water! Then re-inoculated after 3 days and nothing till now....its been another 3 days.
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OPB
Wanderer

Registered: 05/30/15
Posts: 198
Last seen: 2 years, 10 days
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Re: Bunk syringe - reinoculate? [Re: oontribe]
#21909537 - 07/07/15 12:35 PM (8 years, 6 months ago) |
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I can't wait to have a strong supply of agar cultures so that I can just ditch syringes all together.
-------------------- "Wasn't that fun"
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