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OfflineRyeJar
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Freezing contaminated plates
    #21907144 - 07/06/15 08:54 PM (8 years, 6 months ago)

Im trying to isolate a fruitbody at the moment.  I'm on my fourth xfer from fruit with no obvious sectoring yet.  Got home and found the plate that had the best looking characteristics (fast and slightly more rhizo than the others) is peppered with bacteria.  I have not the time to clean a lab space tonight but that plate is going to look horrible tomorrow.  Ever try freezing a bad plate for future transfer (1 day in my case)?


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Re: Freezing contaminated plates [Re: RyeJar]
    #21907168 - 07/06/15 08:57 PM (8 years, 6 months ago)

cubes don't enjoy being frozen you might just have to try it out.


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Onlinerxb
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Re: Freezing contaminated plates [Re: bodhisatta]
    #21907197 - 07/06/15 09:04 PM (8 years, 6 months ago)

try some 35% hydrogen peroxide... i did an experiment with it, and it killed mold and saved the myc... worth a shot.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Re: Freezing contaminated plates [Re: rxb]
    #21907209 - 07/06/15 09:07 PM (8 years, 6 months ago)

mycelium is mold, however cubensis isn't as resilient as most other molds can be.

https://en.wikipedia.org/wiki/Mold

just so you know what you're talking about and stuff.


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OfflineKloo.w
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Re: Freezing contaminated plates [Re: RyeJar]
    #21907229 - 07/06/15 09:12 PM (8 years, 6 months ago)

bad attempt


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OfflineRyeJar
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Re: Freezing contaminated plates [Re: Kloo.w]
    #21907247 - 07/06/15 09:15 PM (8 years, 6 months ago)

Would 35 be cold eniugh slow bacteria considerably?

I guess I kinda knew they didn't like freezing.  Plus when freezing you are supposed to do it like one degree c every minute.


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Onlinerxb
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Re: Freezing contaminated plates [Re: bodhisatta]
    #21907419 - 07/06/15 09:41 PM (8 years, 6 months ago)

Quote:

bodhisatta said:
mycelium is mold, however cubensis isn't as resilient as most other molds can be.

https://en.wikipedia.org/wiki/Mold

just so you know what you're talking about and stuff.




i know exactly what im talking about... im not sure you understood.

i did an experiment i contaminated a live culture of atl#7 with green mold on purpose.

i colonized agar with it, getting both mold and good myc.

i injected 35% hydrogen peroxide at 6cc's ...

it killed the mold, and the ATL#7 survived.

since HP also kills bacteria... and does so, quickly and easily.

you could try this with your problem and you may get better results than freezing.

note this was FOOD GRADE 35% hydrogen peroxide. with NO Other ingredients


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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OfflineRyeJar
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Re: Freezing contaminated plates [Re: rxb]
    #21907502 - 07/06/15 09:54 PM (8 years, 6 months ago)

I don't want to kill the bacteria.  I'm just looking to transfer it but tonight just isn't an option.


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OfflineYaMoonSun
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Re: Freezing contaminated plates [Re: bodhisatta]
    #21907561 - 07/06/15 10:06 PM (8 years, 6 months ago)

I hate to be "that guy" but just because all mold is considered a fungi, does that imply that all fungi is a mold? I can understand a mold having a mycelium, maybe, due to it's fungi nature, but to say that all mycelium is mold seems odd. I hope I didn't go full retard. :shrug:


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OfflineYaMoonSun
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Re: Freezing contaminated plates [Re: rxb]
    #21907563 - 07/06/15 10:07 PM (8 years, 6 months ago)

I hear that ONLY works with cobweb mold


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Re: Freezing contaminated plates [Re: YaMoonSun]
    #21907780 - 07/06/15 11:22 PM (8 years, 6 months ago)

Mold and yeast and mold/yeast like fungi that's all there is you have mold or yeast. C.albicans does both. But is classified a yeast.


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Onlinerxb
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Re: Freezing contaminated plates [Re: YaMoonSun]
    #21908347 - 07/07/15 05:29 AM (8 years, 6 months ago)

Quote:

YaMoonSun said:
I hear that ONLY works with cobweb mold





you hear that about 3% HP not 35% HP


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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OfflineYaMoonSun
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Re: Freezing contaminated plates [Re: rxb]
    #21908389 - 07/07/15 05:54 AM (8 years, 6 months ago)



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Onlinerxb
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Re: Freezing contaminated plates [Re: YaMoonSun]
    #21908390 - 07/07/15 05:55 AM (8 years, 6 months ago)

Quote:

YaMoonSun said:
All hydrogen peroxide :shrug:
http://www.shroomery.org/forums/showflat.php/Number/7329622#7329622




then you heard wrong, and understand very little about hydrogen peroxide.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Onlinerxb
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Re: Freezing contaminated plates [Re: rxb]
    #21908393 - 07/07/15 05:57 AM (8 years, 6 months ago)

that whole thread is talking about drug store brown bottle H202 which is 3% and contains things to stabilize it.

im talking about 35% which is halfway to jet fuel.

thats like night and day.

thats like saying 51% alcohol is the same as 91% alcohol.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


Edited by rxb (07/07/15 05:59 AM)


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Re: Freezing contaminated plates [Re: rxb]
    #21908403 - 07/07/15 06:05 AM (8 years, 6 months ago)

Just put the plate in the fridge,  that should slow things enough for a transfer to be made.

Also while some chemicals like peroxide will kill many things, it will not kill all things. When working with 2D media you should only resort to the use of chemicals or antibiotics when all other attempts fail.


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Re: Freezing contaminated plates [Re: Pastywhyte]
    #21908489 - 07/07/15 06:55 AM (8 years, 6 months ago)

Put 26% in your hand and tell me it won't kill all molds. 26% is similar to what acid does in Hollywood movies. After 30s you won't be able to resist trying to wash it off


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Re: Freezing contaminated plates [Re: bodhisatta] * 1
    #21908500 - 07/07/15 06:58 AM (8 years, 6 months ago)

I wasn't really talking about molds. But at any rate if its gonna kill everything at that concentration, the myc will be killed as well.


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Re: Freezing contaminated plates [Re: Pastywhyte]
    #21909515 - 07/07/15 12:27 PM (8 years, 6 months ago)

Sorry to jack this thread but can you freeze contaminated jars instead of sterilising em in the pc before cleaning em?


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Re: Freezing contaminated plates [Re: oontribe]
    #21909556 - 07/07/15 12:41 PM (8 years, 6 months ago)

Sure just put em in the freezer. But I wouldn't do it myself. PC and be safe.


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Re: Freezing contaminated plates [Re: Pastywhyte]
    #21909774 - 07/07/15 01:36 PM (8 years, 6 months ago)

Thanks pasty :smile:


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OfflineRyeJar
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Re: Freezing contaminated plates [Re: Pastywhyte]
    #21909853 - 07/07/15 01:56 PM (8 years, 6 months ago)

Quote:

Pastywhyte said:
Just put the plate in the fridge,  that should slow things enough for a transfer to be made.




5 hats what I ended up doing.  Thanks pasty.


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Onlinerxb
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Re: Freezing contaminated plates [Re: RyeJar]
    #21911719 - 07/07/15 08:37 PM (8 years, 6 months ago)

i did it with myc and mold the mold died the myc lived,

its something to try sometimes, check behind me and report your results.

the only way we progress this field is to try new things and learn from others, making blanket statements that stuff wont work or wont work well when you havent tried it and dont know, is just useless noise.

i can vouch for it working once.

i dont claim that this is tried and true... and it should be tried with bacterial infections and other contaminants


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Re: Freezing contaminated plates [Re: rxb]
    #21911752 - 07/07/15 08:41 PM (8 years, 6 months ago)

Lots of things work once. I give advice that I know to be tested and true. I use blanket statements when discussing proper procedure. Its nothing personal.


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Onlinerxb
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Re: Freezing contaminated plates [Re: Pastywhyte]
    #21912108 - 07/07/15 09:50 PM (8 years, 6 months ago)

Quote:

Pastywhyte said:
Lots of things work once. I give advice that I know to be tested and true. I use blanket statements when discussing proper procedure. Its nothing personal.




lol... its something sophomoric....since i qualified my suggestion with all the correct details...

and you simply used guesses and speculation to for some reason talk down something which has shown promise and SHOULD be tested.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Re: Freezing contaminated plates [Re: rxb]
    #21912245 - 07/07/15 10:13 PM (8 years, 6 months ago)

Quote:

rxb said:
Quote:

Pastywhyte said:
Lots of things work once. I give advice that I know to be tested and true. I use blanket statements when discussing proper procedure. Its nothing personal.




lol... its something sophomoric....since i qualified my suggestion with all the correct details...

and you simply used guesses and speculation to for some reason talk down something which has shown promise and SHOULD be tested.




Then test it. Many times. Post the results. I have used H2O2 at 3% and found that it inhibited the recovery and the culture was no longer in an aggressive colonizing state. I know of other people who have had the cultures damaged and not able to fruit well afterwards. IMO its not a good practice compared to many other methods of cleaning up cultures and cloning potentially dirty fruits.I can only imagine what may happen with 30% but it seems unnecessary. I give advice based on the way I grow. I will point out flaws as I perceive them with other peoples advice, or at least give my perspective if I disagree with a method. H2O2 can damage the cube mycelium at 3%. That's not guesswork.

We all grow differently. I do things very different with some of the people I respect most here, and will disagree on strategy all the time. Proof is in results. Post yours and I will post mine. So its nothing personal.


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Re: Freezing contaminated plates [Re: Pastywhyte]
    #21912277 - 07/07/15 10:20 PM (8 years, 6 months ago)

was it food grade? because the stuff you put on cuts is not going to work kiddo.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


Edited by rxb (07/07/15 10:21 PM)


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Re: Freezing contaminated plates [Re: rxb]
    #21912290 - 07/07/15 10:24 PM (8 years, 6 months ago)

It was 3% which damaged the myc and the veg state was no longer to my liking. Colonized shitty. 30% can't be any better for the myc. I prefer to not expose my cultures to chemicals. Period.


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OfflineYaMoonSun
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Re: Freezing contaminated plates [Re: rxb]
    #21912292 - 07/07/15 10:24 PM (8 years, 6 months ago)

:canthelpbutlaugh:


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Onlinerxb
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Re: Freezing contaminated plates [Re: Pastywhyte]
    #21912301 - 07/07/15 10:26 PM (8 years, 6 months ago)

Quote:

Pastywhyte said:
It was 3% which damaged the myc and the veg state was no longer to my liking. Colonized shitty. 30% can't be any better for the myc. I prefer to not expose my cultures to chemicals. Period.




the stuff from the drug store isnt JUST hydrogen peroxide...

it has preservatives in it.

which would do bad things to your plates.

so if it wasnt food grade... you didnt do shit.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Onlinerxb
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Re: Freezing contaminated plates [Re: rxb]
    #21912328 - 07/07/15 10:32 PM (8 years, 6 months ago)

this is whats in cvs hydrogen peroxide:

Quote:

Most commercial grades of H2O2 contain chelants and sequestrants which minimize its decomposition under normal storage and handling conditions.

The types of stabilizers used in H2O2 vary between producers and product grades. Common stabilizers include:

Colloidal stannate and sodium pyrophosphate (present at 25 - 250 mg/L) are traditional mainstays.
Organophosphonates (e.g., Monsanto's Dequest products) are increasingly common.
Nitrate (for pH adjustment and corrosion inhibition) and phosphoric acid (for pH adjustment) also are used.
Colloidal silicate is used to sequester metals and thereby minimize H2O2 decomposition in certain applications that depend on the bleaching ability of H2O2 in alkali.




this is what they report
Quote:


Active Ingredient: Hydrogen Peroxide (Stabilized) (3%). Inactive Ingredient: Purified Water.




--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Onlinerxb
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Re: Freezing contaminated plates [Re: rxb]
    #21912339 - 07/07/15 10:35 PM (8 years, 6 months ago)

or you could have just said "no it wasnt food grade and i dont know why i bothered to comment i just wanted to be a negative nancy"


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Re: Freezing contaminated plates [Re: rxb] * 1
    #21912495 - 07/07/15 11:09 PM (8 years, 6 months ago)

Calm down.  You haven't done nearly enough publicly with this method you are advocating to be so defensive. Run some logs a side by side and maybe you stance can be justified.  But until then there are plenty of easy ways to clean a culture without resorting to unproven methods. Those are what I will recommend In the meantime.


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Re: Freezing contaminated plates [Re: Pastywhyte]
    #21912497 - 07/07/15 11:10 PM (8 years, 6 months ago)

Quote:

Pastywhyte said:
Calm down.  You haven't done nearly enough with this method you are advocating to be so defensive. Run some logs a side by side and maybe you stance can be justified.  But until then there are plenty of easy ways to clean a culture without resorting to unproven methods. Those are what I will recommend In the meantime.




im advocating for peer review junior.

thats how it works.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Re: Freezing contaminated plates [Re: rxb] * 1
    #21912499 - 07/07/15 11:11 PM (8 years, 6 months ago)

Quote:

rxb said:
Quote:

Pastywhyte said:
Calm down.  You haven't done nearly enough with this method you are advocating to be so defensive. Run some logs a side by side and maybe you stance can be justified.  But until then there are plenty of easy ways to clean a culture without resorting to unproven methods. Those are what I will recommend In the meantime.




im advocating for peer review junior.

thats how it works.




No you were telling a newer grower to do something that has not been peer reviewed.


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Re: Freezing contaminated plates [Re: Pastywhyte]
    #21912501 - 07/07/15 11:12 PM (8 years, 6 months ago)

no i was telling a new grower something that worked for me, that would likely work better than freezing.

stop being a grouch


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Re: Freezing contaminated plates [Re: rxb] * 1
    #21912505 - 07/07/15 11:13 PM (8 years, 6 months ago)

Stop calling me junior, I'm 68 :oldman:


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Re: Freezing contaminated plates [Re: rxb]
    #21912508 - 07/07/15 11:14 PM (8 years, 6 months ago)

i'll tell you what... never mind.

i'll keep coming up with cool useful and effective shit.

and you keep being a stick in the mud.

its cool.. change nothing..


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Onlinerxb
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Re: Freezing contaminated plates [Re: Pastywhyte]
    #21912512 - 07/07/15 11:14 PM (8 years, 6 months ago)

Quote:

Pastywhyte said:
Stop calling me junior, I'm 68 :oldman:




i will when you stop sounding like someone in 4th grade science class.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Re: Freezing contaminated plates [Re: rxb] * 1
    #21912524 - 07/07/15 11:17 PM (8 years, 6 months ago)

:facepalm:

Okay. You win. OP should do what you said. Hopefully he posts results as it would be nice to see results.  Then maybe it can be advocated without resevation.


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Re: Freezing contaminated plates [Re: Pastywhyte]
    #21912534 - 07/07/15 11:19 PM (8 years, 6 months ago)

nod, its something the op could try, it worked nicely for me. i was actually surprised the myc lived.

OP should not use HP from the drug store if OP tries it. op should use food grade.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Re: Freezing contaminated plates [Re: rxb]
    #21912566 - 07/07/15 11:23 PM (8 years, 6 months ago)

Man,
Quote:

rxb said:
i'll tell you what... never mind.

i'll keep coming up with cool useful and effective shit.

and you keep being a stick in the mud.

its cool.. change nothing..




Pasty, a stick in the mud, HARDLY, man know who you are talking about before spouting off BS!

I would take advice rom Pasty anyday of the week compared to yours, have you even looked into some of Pasty's experiments? I would hardly call him a stick in the mud, even  thought he is 68 :rofl:


--------------------
All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon


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Re: Freezing contaminated plates [Re: Imperfect Iam]
    #21912596 - 07/07/15 11:29 PM (8 years, 6 months ago)

i have, some of its great.

doesnt change the fact that he spouted off bullshit, with regards to HP...

some people know a LOT about one thing and nothing about others.

he proved tonight that he doesnt know where his lines are.

you being a fan of his doesnt change that.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Re: Freezing contaminated plates [Re: rxb] * 1
    #21912662 - 07/07/15 11:41 PM (8 years, 6 months ago)

Wrong. I said that using chemicals to clean up cultures is uneccesary (it is especially with bacteria).

I said that when I did it it impeded recovery. It did.

You claimed results from something you did once and never documented.

You claimed that the reason it didn't work well when I did it was due to the preservatives, yet did nothing to prove it.

Not sure where I went wrong. Guess i feel shit should be we tested before I advocate it to others and I expect others to as well.


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Re: Freezing contaminated plates [Re: Pastywhyte]
    #21912669 - 07/07/15 11:42 PM (8 years, 6 months ago)

jesus you are argumentative

you claimed to have done it... you havent... please fuck off.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Re: Freezing contaminated plates [Re: Pastywhyte] * 1
    #21912678 - 07/07/15 11:43 PM (8 years, 6 months ago)

Quote:

Pastywhyte said:
Just put the plate in the fridge,  that should slow things enough for a transfer to be made.

Also while some chemicals like peroxide will kill many things, it will not kill all things. When working with 2D media you should only resort to the use of chemicals or antibiotics when all other attempts fail.




My first post In this thread. I still fail to see anything in it I will not stand by.


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Re: Freezing contaminated plates [Re: Pastywhyte]
    #21912681 - 07/07/15 11:44 PM (8 years, 6 months ago)

thats clearly not your ONLY post.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Re: Freezing contaminated plates [Re: rxb]
    #21912688 - 07/07/15 11:45 PM (8 years, 6 months ago)

you clearly did not understand food grade vs pharma grade..

you probably do NOW.

and you probably understand NOW that your test has NOTHING to do with what i was talking about.

so stop arguing????


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


Edited by rxb (07/07/15 11:45 PM)


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Re: Freezing contaminated plates [Re: rxb] * 1
    #21912730 - 07/07/15 11:54 PM (8 years, 6 months ago)

Find the one where I say that I used 30%. I was clear from the start I only did it with 3%.

You claimed that it was the preservatives in the H2O2 that damaged the myc. So far you have provided nothing to back that up other than a list of what may be in it. Of course that doesn't prove that the H2O2 was not a factor.  I'd like to think I'm open enough to change my mind if given a good reason to do so. You have yet to provide that.

I also give advice that I know will work. Not something i did once and use someone else's project to test my ideas. I barely even care anymore. OP can do what they want. In the meantime I look forward to your writeup.


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Re: Freezing contaminated plates [Re: Pastywhyte]
    #21912751 - 07/07/15 11:56 PM (8 years, 6 months ago)

you didnt.

and thats not what i said either.

you suck at debate, you are good at agar teks.

im done fighting your strawmen.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Re: Freezing contaminated plates [Re: rxb] * 1
    #21912783 - 07/08/15 12:03 AM (8 years, 6 months ago)

I'm not debating anything. I'm just saying you have proved nothing and you're giving advice based off of a single uncontrolled observation. Show me a strawman, I have made none.


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Re: Freezing contaminated plates [Re: Pastywhyte]
    #21912795 - 07/08/15 12:05 AM (8 years, 6 months ago)

on and on you go, with bullshit, trying to win back some dignity from a fuck up you made a page ago.

just leave off.

you are looking more foolish every post.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Re: Freezing contaminated plates [Re: rxb]
    #21912832 - 07/08/15 12:15 AM (8 years, 6 months ago)

Holy crap.. Idk man all that pastys said this whole thread is, he only gives advice on things with solid proof and evidence to back it up, and for you to make a writeup, show proof, and evidence to back it up before giving advice about it. He actually hasn't really changed anything he's said. You've just been really hostile for him not wanting any bad info spread.

Anywho I agree it'd be cool to see side by sides and what not, but under proper sterile conditions and a good technique, it shouldn't be necessary.


--------------------
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Re: Freezing contaminated plates [Re: Mad Season]
    #21912858 - 07/08/15 12:20 AM (8 years, 6 months ago)

mad, he said he had tried it with 3 percent and it didnt work so 35 cant... he said MANY other things...

im not going to debate the man, he knows he stuck his foot in it.. and i dont want to shame him.

i'm just moving on.

he did something cuz he likes being smart, he over-reached.

over it.

he IS smart... so im not going to eat him apart for using bad debate tactics im just going to point it out.

he said something wrong about something he wasnt educated about, and it was corrected for anyone with a clue.

including probably him.

this really should move on and stop being a shit storm.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Re: Freezing contaminated plates [Re: rxb]
    #21912862 - 07/08/15 12:21 AM (8 years, 6 months ago)

Word. This thread got jacked hard. Haha


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Re: Freezing contaminated plates [Re: Mad Season] * 1
    #21912899 - 07/08/15 12:31 AM (8 years, 6 months ago)

What debate? What tactics?  What other things have I said?  I still doubt high concerntration H2O2 is harmless. I gave a reason which you tried to refute. Yet you still have not proved high concentration H2O2 isn't harmful to the myc. I have not really said anything more.

I think you're reading more into my comments than I am putting into them.


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Re: Freezing contaminated plates [Re: Pastywhyte]
    #21912910 - 07/08/15 12:33 AM (8 years, 6 months ago)

can you please stop melting down...its driving my opinion of you into the dirt.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Re: Freezing contaminated plates [Re: rxb]
    #21912913 - 07/08/15 12:34 AM (8 years, 6 months ago)

oh i think its harmful, it just came back and the mold didnt.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Re: Freezing contaminated plates [Re: rxb]
    #21912954 - 07/08/15 12:47 AM (8 years, 6 months ago)

:popcorn:


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Re: Freezing contaminated plates [Re: RyeJar]
    #21912960 - 07/08/15 12:49 AM (8 years, 6 months ago)

Quote:

RyeJar said:
:popcorn:




harmful in that i dont see it POSSIBLE that it didnt damage some myc.

but theres still not mold on that agar...and there is mycelium


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Re: Freezing contaminated plates [Re: rxb]
    #21912966 - 07/08/15 12:51 AM (8 years, 6 months ago)

I wasnt responding to your last post directly as much as I was the 'shit storm'


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Re: Freezing contaminated plates [Re: RyeJar]
    #21912975 - 07/08/15 12:54 AM (8 years, 6 months ago)

trying to ignore it... science should be forward progress not fighting over little shit :smile: i got too heated early on, shouldnt have accelerated the negativity. i very much doubt there will be any more of me participating in the shit storm.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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OfflineRyeJar
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Re: Freezing contaminated plates [Re: rxb]
    #21916215 - 07/08/15 06:14 PM (8 years, 6 months ago)

True true.  To me it just seems safer to remove healthy mycelium away from contamination in still air then to nuke the dish with my favorite transfer to date.  To each his own though.  If it doesn't make it I'll get out my tetracycline from my fish supplies and go sandwich tek on the bastard.


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