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rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,521
Loc: FREE PSYCHONAUTICA
Last seen: 2 hours, 35 minutes
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Re: Freezing contaminated plates [Re: Pastywhyte]
#21912534 - 07/07/15 11:19 PM (8 years, 6 months ago) |
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nod, its something the op could try, it worked nicely for me. i was actually surprised the myc lived.
OP should not use HP from the drug store if OP tries it. op should use food grade.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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Imperfect Iam
^means imperfect,not I'm perfect


Registered: 03/05/13
Posts: 7,237
Loc: center of the universe
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Re: Freezing contaminated plates [Re: rxb]
#21912566 - 07/07/15 11:23 PM (8 years, 6 months ago) |
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Man,Quote:
rxb said: i'll tell you what... never mind.
i'll keep coming up with cool useful and effective shit.
and you keep being a stick in the mud.
its cool.. change nothing..
Pasty, a stick in the mud, HARDLY, man know who you are talking about before spouting off BS!
I would take advice rom Pasty anyday of the week compared to yours, have you even looked into some of Pasty's experiments? I would hardly call him a stick in the mud, even thought he is 68
-------------------- All you touch, and all you see, is all your life will ever be- Pink Floyd Life is what happens while you're busy making other plans- John Lennon
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rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,521
Loc: FREE PSYCHONAUTICA
Last seen: 2 hours, 35 minutes
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i have, some of its great.
doesnt change the fact that he spouted off bullshit, with regards to HP...
some people know a LOT about one thing and nothing about others.
he proved tonight that he doesnt know where his lines are.
you being a fan of his doesnt change that.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Freezing contaminated plates [Re: rxb] 1
#21912662 - 07/07/15 11:41 PM (8 years, 6 months ago) |
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Wrong. I said that using chemicals to clean up cultures is uneccesary (it is especially with bacteria).
I said that when I did it it impeded recovery. It did.
You claimed results from something you did once and never documented.
You claimed that the reason it didn't work well when I did it was due to the preservatives, yet did nothing to prove it.
Not sure where I went wrong. Guess i feel shit should be we tested before I advocate it to others and I expect others to as well.
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rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,521
Loc: FREE PSYCHONAUTICA
Last seen: 2 hours, 35 minutes
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Re: Freezing contaminated plates [Re: Pastywhyte]
#21912669 - 07/07/15 11:42 PM (8 years, 6 months ago) |
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jesus you are argumentative
you claimed to have done it... you havent... please fuck off.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Freezing contaminated plates [Re: Pastywhyte] 1
#21912678 - 07/07/15 11:43 PM (8 years, 6 months ago) |
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Quote:
Pastywhyte said: Just put the plate in the fridge, that should slow things enough for a transfer to be made.
Also while some chemicals like peroxide will kill many things, it will not kill all things. When working with 2D media you should only resort to the use of chemicals or antibiotics when all other attempts fail.
My first post In this thread. I still fail to see anything in it I will not stand by.
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rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,521
Loc: FREE PSYCHONAUTICA
Last seen: 2 hours, 35 minutes
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Re: Freezing contaminated plates [Re: Pastywhyte]
#21912681 - 07/07/15 11:44 PM (8 years, 6 months ago) |
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thats clearly not your ONLY post.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,521
Loc: FREE PSYCHONAUTICA
Last seen: 2 hours, 35 minutes
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Re: Freezing contaminated plates [Re: rxb]
#21912688 - 07/07/15 11:45 PM (8 years, 6 months ago) |
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you clearly did not understand food grade vs pharma grade..
you probably do NOW.
and you probably understand NOW that your test has NOTHING to do with what i was talking about.
so stop arguing????
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
Edited by rxb (07/07/15 11:45 PM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Freezing contaminated plates [Re: rxb] 1
#21912730 - 07/07/15 11:54 PM (8 years, 6 months ago) |
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Find the one where I say that I used 30%. I was clear from the start I only did it with 3%.
You claimed that it was the preservatives in the H2O2 that damaged the myc. So far you have provided nothing to back that up other than a list of what may be in it. Of course that doesn't prove that the H2O2 was not a factor. I'd like to think I'm open enough to change my mind if given a good reason to do so. You have yet to provide that.
I also give advice that I know will work. Not something i did once and use someone else's project to test my ideas. I barely even care anymore. OP can do what they want. In the meantime I look forward to your writeup.
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rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,521
Loc: FREE PSYCHONAUTICA
Last seen: 2 hours, 35 minutes
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Re: Freezing contaminated plates [Re: Pastywhyte]
#21912751 - 07/07/15 11:56 PM (8 years, 6 months ago) |
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you didnt.
and thats not what i said either.
you suck at debate, you are good at agar teks.
im done fighting your strawmen.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Freezing contaminated plates [Re: rxb] 1
#21912783 - 07/08/15 12:03 AM (8 years, 6 months ago) |
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I'm not debating anything. I'm just saying you have proved nothing and you're giving advice based off of a single uncontrolled observation. Show me a strawman, I have made none.
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rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,521
Loc: FREE PSYCHONAUTICA
Last seen: 2 hours, 35 minutes
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Re: Freezing contaminated plates [Re: Pastywhyte]
#21912795 - 07/08/15 12:05 AM (8 years, 6 months ago) |
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on and on you go, with bullshit, trying to win back some dignity from a fuck up you made a page ago.
just leave off.
you are looking more foolish every post.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Freezing contaminated plates [Re: rxb]
#21912832 - 07/08/15 12:15 AM (8 years, 6 months ago) |
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Holy crap.. Idk man all that pastys said this whole thread is, he only gives advice on things with solid proof and evidence to back it up, and for you to make a writeup, show proof, and evidence to back it up before giving advice about it. He actually hasn't really changed anything he's said. You've just been really hostile for him not wanting any bad info spread.
Anywho I agree it'd be cool to see side by sides and what not, but under proper sterile conditions and a good technique, it shouldn't be necessary.
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rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,521
Loc: FREE PSYCHONAUTICA
Last seen: 2 hours, 35 minutes
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Re: Freezing contaminated plates [Re: Mad Season]
#21912858 - 07/08/15 12:20 AM (8 years, 6 months ago) |
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mad, he said he had tried it with 3 percent and it didnt work so 35 cant... he said MANY other things...
im not going to debate the man, he knows he stuck his foot in it.. and i dont want to shame him.
i'm just moving on.
he did something cuz he likes being smart, he over-reached.
over it.
he IS smart... so im not going to eat him apart for using bad debate tactics im just going to point it out.
he said something wrong about something he wasnt educated about, and it was corrected for anyone with a clue.
including probably him.
this really should move on and stop being a shit storm.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Freezing contaminated plates [Re: rxb]
#21912862 - 07/08/15 12:21 AM (8 years, 6 months ago) |
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Word. This thread got jacked hard. Haha
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Freezing contaminated plates [Re: Mad Season] 1
#21912899 - 07/08/15 12:31 AM (8 years, 6 months ago) |
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What debate? What tactics? What other things have I said? I still doubt high concerntration H2O2 is harmless. I gave a reason which you tried to refute. Yet you still have not proved high concentration H2O2 isn't harmful to the myc. I have not really said anything more.
I think you're reading more into my comments than I am putting into them.
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rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,521
Loc: FREE PSYCHONAUTICA
Last seen: 2 hours, 35 minutes
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Re: Freezing contaminated plates [Re: Pastywhyte]
#21912910 - 07/08/15 12:33 AM (8 years, 6 months ago) |
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can you please stop melting down...its driving my opinion of you into the dirt.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,521
Loc: FREE PSYCHONAUTICA
Last seen: 2 hours, 35 minutes
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Re: Freezing contaminated plates [Re: rxb]
#21912913 - 07/08/15 12:34 AM (8 years, 6 months ago) |
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oh i think its harmful, it just came back and the mold didnt.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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RyeJar
StrangerDanger


Registered: 04/16/15
Posts: 523
Last seen: 9 months, 30 days
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Re: Freezing contaminated plates [Re: rxb]
#21912954 - 07/08/15 12:47 AM (8 years, 6 months ago) |
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rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,521
Loc: FREE PSYCHONAUTICA
Last seen: 2 hours, 35 minutes
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Re: Freezing contaminated plates [Re: RyeJar]
#21912960 - 07/08/15 12:49 AM (8 years, 6 months ago) |
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Quote:
RyeJar said:

harmful in that i dont see it POSSIBLE that it didnt damage some myc.
but theres still not mold on that agar...and there is mycelium
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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