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Invisibleoontribe
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Re: Freezing contaminated plates [Re: Pastywhyte]
    #21909774 - 07/07/15 01:36 PM (8 years, 6 months ago)

Thanks pasty :smile:


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OfflineRyeJar
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Re: Freezing contaminated plates [Re: Pastywhyte]
    #21909853 - 07/07/15 01:56 PM (8 years, 6 months ago)

Quote:

Pastywhyte said:
Just put the plate in the fridge,  that should slow things enough for a transfer to be made.




5 hats what I ended up doing.  Thanks pasty.


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Onlinerxb
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Re: Freezing contaminated plates [Re: RyeJar]
    #21911719 - 07/07/15 08:37 PM (8 years, 6 months ago)

i did it with myc and mold the mold died the myc lived,

its something to try sometimes, check behind me and report your results.

the only way we progress this field is to try new things and learn from others, making blanket statements that stuff wont work or wont work well when you havent tried it and dont know, is just useless noise.

i can vouch for it working once.

i dont claim that this is tried and true... and it should be tried with bacterial infections and other contaminants


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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InvisiblePastywhyteMDiscord
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Re: Freezing contaminated plates [Re: rxb]
    #21911752 - 07/07/15 08:41 PM (8 years, 6 months ago)

Lots of things work once. I give advice that I know to be tested and true. I use blanket statements when discussing proper procedure. Its nothing personal.


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Onlinerxb
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Re: Freezing contaminated plates [Re: Pastywhyte]
    #21912108 - 07/07/15 09:50 PM (8 years, 6 months ago)

Quote:

Pastywhyte said:
Lots of things work once. I give advice that I know to be tested and true. I use blanket statements when discussing proper procedure. Its nothing personal.




lol... its something sophomoric....since i qualified my suggestion with all the correct details...

and you simply used guesses and speculation to for some reason talk down something which has shown promise and SHOULD be tested.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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InvisiblePastywhyteMDiscord
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Re: Freezing contaminated plates [Re: rxb]
    #21912245 - 07/07/15 10:13 PM (8 years, 6 months ago)

Quote:

rxb said:
Quote:

Pastywhyte said:
Lots of things work once. I give advice that I know to be tested and true. I use blanket statements when discussing proper procedure. Its nothing personal.




lol... its something sophomoric....since i qualified my suggestion with all the correct details...

and you simply used guesses and speculation to for some reason talk down something which has shown promise and SHOULD be tested.




Then test it. Many times. Post the results. I have used H2O2 at 3% and found that it inhibited the recovery and the culture was no longer in an aggressive colonizing state. I know of other people who have had the cultures damaged and not able to fruit well afterwards. IMO its not a good practice compared to many other methods of cleaning up cultures and cloning potentially dirty fruits.I can only imagine what may happen with 30% but it seems unnecessary. I give advice based on the way I grow. I will point out flaws as I perceive them with other peoples advice, or at least give my perspective if I disagree with a method. H2O2 can damage the cube mycelium at 3%. That's not guesswork.

We all grow differently. I do things very different with some of the people I respect most here, and will disagree on strategy all the time. Proof is in results. Post yours and I will post mine. So its nothing personal.


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Onlinerxb
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Re: Freezing contaminated plates [Re: Pastywhyte]
    #21912277 - 07/07/15 10:20 PM (8 years, 6 months ago)

was it food grade? because the stuff you put on cuts is not going to work kiddo.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


Edited by rxb (07/07/15 10:21 PM)


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InvisiblePastywhyteMDiscord
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Re: Freezing contaminated plates [Re: rxb]
    #21912290 - 07/07/15 10:24 PM (8 years, 6 months ago)

It was 3% which damaged the myc and the veg state was no longer to my liking. Colonized shitty. 30% can't be any better for the myc. I prefer to not expose my cultures to chemicals. Period.


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OfflineYaMoonSun
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Re: Freezing contaminated plates [Re: rxb]
    #21912292 - 07/07/15 10:24 PM (8 years, 6 months ago)

:canthelpbutlaugh:


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Onlinerxb
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Re: Freezing contaminated plates [Re: Pastywhyte]
    #21912301 - 07/07/15 10:26 PM (8 years, 6 months ago)

Quote:

Pastywhyte said:
It was 3% which damaged the myc and the veg state was no longer to my liking. Colonized shitty. 30% can't be any better for the myc. I prefer to not expose my cultures to chemicals. Period.




the stuff from the drug store isnt JUST hydrogen peroxide...

it has preservatives in it.

which would do bad things to your plates.

so if it wasnt food grade... you didnt do shit.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Onlinerxb
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Re: Freezing contaminated plates [Re: rxb]
    #21912328 - 07/07/15 10:32 PM (8 years, 6 months ago)

this is whats in cvs hydrogen peroxide:

Quote:

Most commercial grades of H2O2 contain chelants and sequestrants which minimize its decomposition under normal storage and handling conditions.

The types of stabilizers used in H2O2 vary between producers and product grades. Common stabilizers include:

Colloidal stannate and sodium pyrophosphate (present at 25 - 250 mg/L) are traditional mainstays.
Organophosphonates (e.g., Monsanto's Dequest products) are increasingly common.
Nitrate (for pH adjustment and corrosion inhibition) and phosphoric acid (for pH adjustment) also are used.
Colloidal silicate is used to sequester metals and thereby minimize H2O2 decomposition in certain applications that depend on the bleaching ability of H2O2 in alkali.




this is what they report
Quote:


Active Ingredient: Hydrogen Peroxide (Stabilized) (3%). Inactive Ingredient: Purified Water.




--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Onlinerxb
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Re: Freezing contaminated plates [Re: rxb]
    #21912339 - 07/07/15 10:35 PM (8 years, 6 months ago)

or you could have just said "no it wasnt food grade and i dont know why i bothered to comment i just wanted to be a negative nancy"


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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InvisiblePastywhyteMDiscord
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Re: Freezing contaminated plates [Re: rxb] * 1
    #21912495 - 07/07/15 11:09 PM (8 years, 6 months ago)

Calm down.  You haven't done nearly enough publicly with this method you are advocating to be so defensive. Run some logs a side by side and maybe you stance can be justified.  But until then there are plenty of easy ways to clean a culture without resorting to unproven methods. Those are what I will recommend In the meantime.


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Onlinerxb
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Re: Freezing contaminated plates [Re: Pastywhyte]
    #21912497 - 07/07/15 11:10 PM (8 years, 6 months ago)

Quote:

Pastywhyte said:
Calm down.  You haven't done nearly enough with this method you are advocating to be so defensive. Run some logs a side by side and maybe you stance can be justified.  But until then there are plenty of easy ways to clean a culture without resorting to unproven methods. Those are what I will recommend In the meantime.




im advocating for peer review junior.

thats how it works.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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InvisiblePastywhyteMDiscord
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Re: Freezing contaminated plates [Re: rxb] * 1
    #21912499 - 07/07/15 11:11 PM (8 years, 6 months ago)

Quote:

rxb said:
Quote:

Pastywhyte said:
Calm down.  You haven't done nearly enough with this method you are advocating to be so defensive. Run some logs a side by side and maybe you stance can be justified.  But until then there are plenty of easy ways to clean a culture without resorting to unproven methods. Those are what I will recommend In the meantime.




im advocating for peer review junior.

thats how it works.




No you were telling a newer grower to do something that has not been peer reviewed.


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Onlinerxb
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Re: Freezing contaminated plates [Re: Pastywhyte]
    #21912501 - 07/07/15 11:12 PM (8 years, 6 months ago)

no i was telling a new grower something that worked for me, that would likely work better than freezing.

stop being a grouch


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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InvisiblePastywhyteMDiscord
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Re: Freezing contaminated plates [Re: rxb] * 1
    #21912505 - 07/07/15 11:13 PM (8 years, 6 months ago)

Stop calling me junior, I'm 68 :oldman:


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Onlinerxb
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Re: Freezing contaminated plates [Re: rxb]
    #21912508 - 07/07/15 11:14 PM (8 years, 6 months ago)

i'll tell you what... never mind.

i'll keep coming up with cool useful and effective shit.

and you keep being a stick in the mud.

its cool.. change nothing..


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


Extras: Filter Print Post Top
Onlinerxb
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Re: Freezing contaminated plates [Re: Pastywhyte]
    #21912512 - 07/07/15 11:14 PM (8 years, 6 months ago)

Quote:

Pastywhyte said:
Stop calling me junior, I'm 68 :oldman:




i will when you stop sounding like someone in 4th grade science class.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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InvisiblePastywhyteMDiscord
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Re: Freezing contaminated plates [Re: rxb] * 1
    #21912524 - 07/07/15 11:17 PM (8 years, 6 months ago)

:facepalm:

Okay. You win. OP should do what you said. Hopefully he posts results as it would be nice to see results.  Then maybe it can be advocated without resevation.


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