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RyeJar
StrangerDanger


Registered: 04/16/15
Posts: 523
Last seen: 9 months, 30 days
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Freezing contaminated plates
#21907144 - 07/06/15 08:54 PM (8 years, 6 months ago) |
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Im trying to isolate a fruitbody at the moment. I'm on my fourth xfer from fruit with no obvious sectoring yet. Got home and found the plate that had the best looking characteristics (fast and slightly more rhizo than the others) is peppered with bacteria. I have not the time to clean a lab space tonight but that plate is going to look horrible tomorrow. Ever try freezing a bad plate for future transfer (1 day in my case)?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Freezing contaminated plates [Re: RyeJar]
#21907168 - 07/06/15 08:57 PM (8 years, 6 months ago) |
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cubes don't enjoy being frozen you might just have to try it out.
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rxb
n00b-sabot



Registered: 08/24/13
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Re: Freezing contaminated plates [Re: bodhisatta]
#21907197 - 07/06/15 09:04 PM (8 years, 6 months ago) |
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try some 35% hydrogen peroxide... i did an experiment with it, and it killed mold and saved the myc... worth a shot.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Freezing contaminated plates [Re: rxb]
#21907209 - 07/06/15 09:07 PM (8 years, 6 months ago) |
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mycelium is mold, however cubensis isn't as resilient as most other molds can be.
https://en.wikipedia.org/wiki/Mold
just so you know what you're talking about and stuff.
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Kloo.w
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Registered: 03/04/15
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Re: Freezing contaminated plates [Re: RyeJar]
#21907229 - 07/06/15 09:12 PM (8 years, 6 months ago) |
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bad attempt
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RyeJar
StrangerDanger


Registered: 04/16/15
Posts: 523
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Re: Freezing contaminated plates [Re: Kloo.w]
#21907247 - 07/06/15 09:15 PM (8 years, 6 months ago) |
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Would 35 be cold eniugh slow bacteria considerably?
I guess I kinda knew they didn't like freezing. Plus when freezing you are supposed to do it like one degree c every minute.
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rxb
n00b-sabot



Registered: 08/24/13
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Re: Freezing contaminated plates [Re: bodhisatta]
#21907419 - 07/06/15 09:41 PM (8 years, 6 months ago) |
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Quote:
bodhisatta said: mycelium is mold, however cubensis isn't as resilient as most other molds can be.
https://en.wikipedia.org/wiki/Mold
just so you know what you're talking about and stuff.
i know exactly what im talking about... im not sure you understood.
i did an experiment i contaminated a live culture of atl#7 with green mold on purpose.
i colonized agar with it, getting both mold and good myc.
i injected 35% hydrogen peroxide at 6cc's ...
it killed the mold, and the ATL#7 survived.
since HP also kills bacteria... and does so, quickly and easily.
you could try this with your problem and you may get better results than freezing.
note this was FOOD GRADE 35% hydrogen peroxide. with NO Other ingredients
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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RyeJar
StrangerDanger


Registered: 04/16/15
Posts: 523
Last seen: 9 months, 30 days
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Re: Freezing contaminated plates [Re: rxb]
#21907502 - 07/06/15 09:54 PM (8 years, 6 months ago) |
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I don't want to kill the bacteria. I'm just looking to transfer it but tonight just isn't an option.
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
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Re: Freezing contaminated plates [Re: bodhisatta]
#21907561 - 07/06/15 10:06 PM (8 years, 6 months ago) |
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I hate to be "that guy" but just because all mold is considered a fungi, does that imply that all fungi is a mold? I can understand a mold having a mycelium, maybe, due to it's fungi nature, but to say that all mycelium is mold seems odd. I hope I didn't go full retard.
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
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Re: Freezing contaminated plates [Re: rxb]
#21907563 - 07/06/15 10:07 PM (8 years, 6 months ago) |
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I hear that ONLY works with cobweb mold
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Freezing contaminated plates [Re: YaMoonSun]
#21907780 - 07/06/15 11:22 PM (8 years, 6 months ago) |
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Mold and yeast and mold/yeast like fungi that's all there is you have mold or yeast. C.albicans does both. But is classified a yeast.
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rxb
n00b-sabot



Registered: 08/24/13
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Re: Freezing contaminated plates [Re: YaMoonSun]
#21908347 - 07/07/15 05:29 AM (8 years, 6 months ago) |
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Quote:
YaMoonSun said: I hear that ONLY works with cobweb mold
you hear that about 3% HP not 35% HP
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Re: Freezing contaminated plates [Re: rxb]
#21908389 - 07/07/15 05:54 AM (8 years, 6 months ago) |
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rxb
n00b-sabot



Registered: 08/24/13
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Re: Freezing contaminated plates [Re: YaMoonSun]
#21908390 - 07/07/15 05:55 AM (8 years, 6 months ago) |
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Quote:
YaMoonSun said: All hydrogen peroxide  http://www.shroomery.org/forums/showflat.php/Number/7329622#7329622
then you heard wrong, and understand very little about hydrogen peroxide.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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rxb
n00b-sabot



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Posts: 9,525
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Re: Freezing contaminated plates [Re: rxb]
#21908393 - 07/07/15 05:57 AM (8 years, 6 months ago) |
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that whole thread is talking about drug store brown bottle H202 which is 3% and contains things to stabilize it.
im talking about 35% which is halfway to jet fuel.
thats like night and day.
thats like saying 51% alcohol is the same as 91% alcohol.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
Edited by rxb (07/07/15 05:59 AM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Freezing contaminated plates [Re: rxb]
#21908403 - 07/07/15 06:05 AM (8 years, 6 months ago) |
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Just put the plate in the fridge, that should slow things enough for a transfer to be made.
Also while some chemicals like peroxide will kill many things, it will not kill all things. When working with 2D media you should only resort to the use of chemicals or antibiotics when all other attempts fail.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Freezing contaminated plates [Re: Pastywhyte]
#21908489 - 07/07/15 06:55 AM (8 years, 6 months ago) |
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Put 26% in your hand and tell me it won't kill all molds. 26% is similar to what acid does in Hollywood movies. After 30s you won't be able to resist trying to wash it off
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Freezing contaminated plates [Re: bodhisatta] 1
#21908500 - 07/07/15 06:58 AM (8 years, 6 months ago) |
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I wasn't really talking about molds. But at any rate if its gonna kill everything at that concentration, the myc will be killed as well.
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oontribe

Registered: 01/14/15
Posts: 3,570
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Re: Freezing contaminated plates [Re: Pastywhyte]
#21909515 - 07/07/15 12:27 PM (8 years, 6 months ago) |
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Sorry to jack this thread but can you freeze contaminated jars instead of sterilising em in the pc before cleaning em?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Freezing contaminated plates [Re: oontribe]
#21909556 - 07/07/15 12:41 PM (8 years, 6 months ago) |
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Sure just put em in the freezer. But I wouldn't do it myself. PC and be safe.
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