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Offlinemore4u2c
Mad Hatter

Registered: 07/08/14
Posts: 470
Last seen: 2 days, 10 hours
Mushroom grew in agar plate should I clone him?
    #21905400 - 07/06/15 02:05 PM (8 years, 6 months ago)

So my last grow got contaminated and I'm sick and tired of it lol I have totally clean jars of rye and totally clean mono samples from agar and still get all contaminated going to bulk so it's my coir that's dirty I did all the correct pasteration and everything and it just doesn't want to work for me.

So as I was going thru some old agar dishes I found one with a huge shroom inside and hella lil pins everywhere (very old dish) not any contamination at all inside so I'm thinking seeing how I suck at bulk I should try BRF for the first time can I use this invetro shroom genetics and clone him would he be very strong and potent seeing how he survived in just a lil agar dish? And how would you knock up BRF with a tissue sample is that possible or do I need to put them into a LC syringe to knock BRF?

Any help would be appreciated


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InvisibleChilldog
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Registered: 06/30/15
Posts: 53
Loc: the house
Re: Mushroom grew in agar plate should I clone him? [Re: more4u2c]
    #21905417 - 07/06/15 02:11 PM (8 years, 6 months ago)

I would clone him to another agar plate. You could drop tissue in a jar, however not the best idea.

For a brf cake you would have to have a lid with filtered GE as apposed to the dry vermiculite barrier.


Edited by Chilldog (07/06/15 02:25 PM)


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InvisibleJvF
Fletcher Detcher


Registered: 02/13/14
Posts: 2,662
Loc: Chicago Flag
Re: Mushroom grew in agar plate should I clone him? [Re: Chilldog]
    #21905460 - 07/06/15 02:24 PM (8 years, 6 months ago)

:popcorn:


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InvisibleMad Season
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Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
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Re: Mushroom grew in agar plate should I clone him? [Re: Chilldog]
    #21905466 - 07/06/15 02:25 PM (8 years, 6 months ago)

Quote:

Chilldog said:
I would clone him to another agar plate. You could drop tissue in a jar, however not the best idea.

For a brf cake you would have to have a lid with filtered GE as apposed to the dry vermiculite barrier.



That's why you clone to a plate and then turn that plate to a liquid. Either li or lc.


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Offlinemore4u2c
Mad Hatter

Registered: 07/08/14
Posts: 470
Last seen: 2 days, 10 hours
Re: Mushroom grew in agar plate should I clone him? [Re: Mad Season]
    #21906396 - 07/06/15 06:19 PM (8 years, 6 months ago)

So it's ok to do brf with a tevk lid like I do grains? Also can I still use my mono tub as is or would I need to add a bed of perlite? I tried a shotgun set up once and it sucked didn't work at all idky anywho yeah I was going to clone the agar shroom into new agar and maybe even do 2 more transfers before inoculation of brf


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OfflineTravelAgency
The ongoing "wow"

Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 23 days
Re: Mushroom grew in agar plate should I clone him? [Re: Mad Season]
    #21907276 - 07/06/15 09:17 PM (8 years, 6 months ago)

Quote:

Mad Season said:
Quote:

Chilldog said:
I would clone him to another agar plate. You could drop tissue in a jar, however not the best idea.

For a brf cake you would have to have a lid with filtered GE as apposed to the dry vermiculite barrier.



That's why you clone to a plate and then turn that plate to a liquid. Either li or lc.





Ok, off topic a little but I've been confused by this a bit- what is the difference between LI and LC? From what I gather LC is more "alive" but that's about as far as I understand it.


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Invisibleamp244
Sporocarp Stretching


Registered: 08/05/08
Posts: 1,336
Re: Mushroom grew in agar plate should I clone him? [Re: TravelAgency]
    #21907707 - 07/06/15 10:51 PM (8 years, 6 months ago)

Liquid inoculate is simply a blended/fragmented mycelial mass suspened in water. The water is used as a means to inoculate substrate/spawn media. By blending/fragmenting the mycelium (a colonized Petri dish, brf cake, etc) you are increasing the number of potential inoculation points when you inoculate. This is the same principle as breaking up a grain jar before spawning to a bulk sub. So instead of cutting a single agar wedge, you blend up a whole (or most of) agar plate with water to increase the inoculation points. The more water you use the further you can "stretch" this inoculate, but the fewer inoculation points per ml you will have.

Liquid culture is a nutrient rich aqueous solution where the mycelium will proliferate and expand its mass. It is used as a means of generating mycelium as well as serving as a liquid inoculate, but it is important to break up the mycelial mass before using as inoculate. Also, the nutrient rich solution may foster contaminants and will eventually fall victim to senescence.

So in other words LI: just water, LC: water + food.


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OfflineTravelAgency
The ongoing "wow"

Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 23 days
Re: Mushroom grew in agar plate should I clone him? [Re: amp244]
    #21907853 - 07/06/15 11:39 PM (8 years, 6 months ago)

Ok thanks for that- it was around what I thought but I see people talk about both, often in the same breath.


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