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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 20 days
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Quote:
spacechildo said:
Quote:
Machiavelliavore said: Coir Verm is a very easy substrate to work with. If it contaminates, it was probably bad spawn, and franks tek is legit.
However, I would suggest you learn on invitro V-tek. All you need for it is grass seed and quart PP5 containers. I have found grass seed to be a very forgiving grain in terms of contamination from spore syringes,
lol, show me a pic that tells me why he should do in vitro cased grains instead of a cvg bulg grow! 
and rgs doesnt contam as easily? You're making stuff up dude.
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Machiavelliavore said: I can't imagine 20% verm has any drastic textural or water capacity effect.
With a brick of coir you can add like ~guessing~ 3-3.5L water and buckettek it thought free.
so you've never done the bucket tek nor seen how verm fluffs up a bulk sub? How are you gonna help anyone with something you havent even done yourself?
As for using straight coir, adding 2qt vermiculite seems to add a particle half the size of a rice grain about every couple mm, no way that low of a density of verm has a massive effect on texture. Coir is fluffy when properly hydrated on its own. Not saying there is no reason to add verm or that it isn't better, just that it's not mandatory.
Try using the search function, you'll find plenty of accounts of success with straight coir.  Here's two TC's confirming it http://www.shroomery.org/forums/showflat.php/Number/20101656 http://www.shroomery.org/forums/showflat.php/Number/6004845 Tek
As for grass seed, I've never had an MS inocultion fail on grass seed. Invitro vtek is a dirt cheap way to get your feet wet at virtually no cost, get experience with working on grains, knowing how they should look, and how to prepare them, with low penalty for failure. Grass seed prep in plastics is extremely forgiving, unlike in glass.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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kibblesnibble
ambitious learner



Registered: 06/28/15
Posts: 8
Loc: 'murica
Last seen: 8 years, 6 months
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i am planning on using a SAB, what is your opinion on polyfil lids instead of RTV? polyfil is what the tek i am following suggests.
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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well of course straight coir works fine I never said otherwise what I commented on was you saying there's not much diff in texture between straight coir and CV.
Quote:
Machiavelliavore said: Grass seed prep in plastics is extremely forgiving, unlike in glass.
say what? so contams wont germinate or exist in plastic? I think you just need more exp. this makes 0 sense..
op; Polyfill is for the needed GE, rtv is for the optional SHIP.
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kibblesnibble
ambitious learner



Registered: 06/28/15
Posts: 8
Loc: 'murica
Last seen: 8 years, 6 months
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so do you think it would be in theory, alright to use a MS syringe on grain (WBS) and just make extra jars in case some contam, and it would still be successful?
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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yes of course. agar is just great because you KNOW you have a clean inoculant and dont have to deal with the whole "is this clean, is this clean" as a newb.
your own sterile tech could still fail but chances are you'd spot it on agar before putting it to grain anyway.
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 20 days
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Quote:
spacechildo said: well of course straight coir works fine I never said otherwise what I commented on was you saying there's not much diff in texture between straight coir and CV.
Quote:
Machiavelliavore said: Grass seed prep in plastics is extremely forgiving, unlike in glass.
say what? so contams wont germinate or exist in plastic? I think you just need more exp. this makes 0 sense..
op; Polyfill is for the needed GE, rtv is for the optional SHIP.
It's easy to overcook grass seed in glass. The starch bakes out and it gets clumpy and more prone to contamination. It's hard to get something that isn't usable with plastic due to the shorter cook time and faster cooldown.
Coir texture is fairly similar w or w.o verm IMO. I've hydrated some coir on its own before adding any verm, then microwave pasturized it. I'll use straight coir next time out of curiosity. Feels more like verm particles lodged between fibers of coir with 80/20, while 50/50 verm whatever mixes are more like coated verm particles.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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plastic/glass dont have shorter cook times. if the seeds burst they were too wet going in.
Quote:
Machiavelliavore said: Coir texture is fairly similar w or w.o verm IMO.
that's so weird, its the complete opposite for me. I can even play around with verm% in my cvg casing layers because its so different..
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Green Bastard
Stranger

Registered: 02/26/14
Posts: 199
Loc: Canada
Last seen: 3 years, 8 months
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Quote:
Machiavelliavore said:
Coir texture is fairly similar w or w.o verm IMO. I've hydrated some coir on its own before adding any verm, then microwave pasturized it. I'll use straight coir next time out of curiosity. Feels more like verm particles lodged between fibers of coir with 80/20, while 50/50 verm whatever mixes are more like coated verm particles.
So not my experience with coir n verm. Sounds like you're using a super fine grade. You might consider using a medium, or coarse grade. I find it helps keeps my sub much fluffier. Much easier to make a non clumpy homogenous bucket tek...
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 20 days
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I believe you are correct and it is fine grade. I didn't have a lot of choice in the matter. It also seems to be above field capacity when hydrated @ 2:1 verm water.
As long as I don't overhydrate it, I find that the 80/20 coir verm substrate is just fine with my verm. 4.5qt is the most I'd dare hydrate my 80/20, though I'm thinking 4-4.25 is better.
I'm gonna try straight coir next time. One brick would be just about right for two of minis. I doubt it makes a particularly good fruiting surface though, so I'll prolly throw some 50/50+ ontop.
Re: Grass seed prep in plastic The combination of plastic containers heating and cooling faster and the reduced PC times, and extra shake room for smaller quantities are what make it forgiving
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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