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Offlineknomadic_niki
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Isolating multispore cultures
    #21900199 - 07/05/15 09:25 AM (8 years, 6 months ago)

So, I isolated a multispore culture of Maz and it took 18 days to fully colonize a coco coir-based supplemented substrate at 75-80 degrees incubation temp and a 20% inoculation rate. Compared to other people's grows, that seems like a long time to me. Would that be a factor to consider in selecting an isolate? Do multispores tend to colonize faster than isolated cultures? I've never worked with this species before, so I don't know what is normal and what's not. For oysters and lion's mane, two weeks is a little long. For reishi and shiitake, though, that is quick...any constructive feedback would be appreciated


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Edited by knomadic_niki (07/05/15 04:46 PM)


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Offlinematsc
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Re: Isolating multispore cultures [Re: knomadic_niki]
    #21901358 - 07/05/15 02:42 PM (8 years, 6 months ago)

I think peat is somewhat unusual substrate choice, perhaps that has something to do with it? I'm afraid I don't really know specifics to help.


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Offlineknomadic_niki
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Re: Isolating multispore cultures [Re: matsc]
    #21901448 - 07/05/15 02:57 PM (8 years, 6 months ago)

oh, crap. i didn't mean peat, i meant coco. i always mix those two up. i'll edit the OP


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Offlinebryanbzl
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Re: Isolating multispore cultures [Re: knomadic_niki]
    #21901760 - 07/05/15 04:17 PM (8 years, 6 months ago)

It is hard to tell anything about the culture without knowing much about the specifics regarding things such as your spawn ratio, moisture content.... etc. make sure to give more information in order to get a better answer. I am not sure - but I think this falls under the general cultivation forum and not advanced mycology. not sure on that part though. Hope this helps.


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bzl

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------
"From 1898 through to 1910 heroin was marketed as a non-addictive morphine substitute and cough medicine for children."

conclusion:
poor fucking children of the early 1900's.


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Offlinebryanbzl
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Re: Isolating multispore cultures [Re: bryanbzl]
    #21901777 - 07/05/15 04:22 PM (8 years, 6 months ago)

my apologies on not noticing the spawn-ratio in your OP. The thing that effected my colonization times the most was dialing down moisture content - even in grain jars. For the most part, As long as the medium is hydrated, I make sure to be far more on the drier side than anything. It could just be that your particular isolate isn't good in the speed department.


--------------------
Cheers,

bzl

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------
"From 1898 through to 1910 heroin was marketed as a non-addictive morphine substitute and cough medicine for children."

conclusion:
poor fucking children of the early 1900's.


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Offlineknomadic_niki
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Re: Isolating multispore cultures [Re: bryanbzl]
    #21901797 - 07/05/15 04:25 PM (8 years, 6 months ago)

my spawn ratio is inculded in the OP. moisture content is about 55%... i think isolating cultures is fairly advanced but who is to judge. i just wanted an advanced audience. some of the mush cult folks are rude. here is a link to my process if that helps:

http://www.shroomery.org/forums/showflat.php/Number/21801980

i'm really just wanting to know the average spawn run time for Maz on coco coir and if different isolates have different spawn run times. this is the first time i've grown this species and the first time i've isolated a strain from a multispore culture so Im not really sure what to expect.


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Edited by knomadic_niki (07/05/15 04:26 PM)


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Offlinebryanbzl
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Re: Isolating multispore cultures [Re: knomadic_niki]
    #21901893 - 07/05/15 04:51 PM (8 years, 6 months ago)

Yea I understand. I just think it really is hard for anyone to answer your question because colonization time varies due to many factors. In terms of water content. saying 55% is really hard for me to quantify any opinion over. all I would say is make sure it is more on the dry side then wet. also I make sure that my subs are not overly compressed as well. IME 2 weeks seems a tad bit long but it could be that specific strains genetics. in the future do a side by side with a control or comparison so that you can draw out your own conclusions easily. That's what I do in order to take control of a lot of variable factors. I Hope this helps you understand things a little better.


--------------------
Cheers,

bzl

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------
"From 1898 through to 1910 heroin was marketed as a non-addictive morphine substitute and cough medicine for children."

conclusion:
poor fucking children of the early 1900's.


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Offlineknomadic_niki
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Re: Isolating multispore cultures [Re: bryanbzl]
    #21901906 - 07/05/15 04:55 PM (8 years, 6 months ago)

my experimental variable this time around was substrate supplements. maybe next time i'll do isolates as a variable. thanks for your input


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Offlinematsc
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Re: Isolating multispore cultures [Re: knomadic_niki]
    #21902038 - 07/05/15 05:35 PM (8 years, 6 months ago)

The problem with mushroom "varieties" is they're basically arbitrary. Given that they are almost exclusively distributed as spores, genetic variation is sky high. The names are just stuck on for marketing and historical purposes. Every single spore is a little different from one another, so once germinated, you don't have a single variety, but hundreds of thousands.

Consequently, even if one does isolate a single clone from the mass, it will most likely be quite different from another isolate, even if its from the same named type (or for that matter, even if taken from the same spore print). Its very difficult to nail down any specifics for a strain other than your own personal collection. Now, a few cases do exist of course, such as the wonkyness of PE and its relatives, the red spored varieties, etc, but by and large it will be a crap shoot.

I know that doesn't really help you much, but its about the best I have to offer.


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Offlinebryanbzl
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Re: Isolating multispore cultures [Re: matsc]
    #21902086 - 07/05/15 05:46 PM (8 years, 6 months ago)

Quote:

matsc said:
The problem with mushroom "varieties" is they're basically arbitrary. Given that they are almost exclusively distributed as spores, genetic variation is sky high. The names are just stuck on for marketing and historical purposes. Every single spore is a little different from one another, so once germinated, you don't have a single variety, but hundreds of thousands.

Consequently, even if one does isolate a single clone from the mass, it will most likely be quite different from another isolate, even if its from the same named type (or for that matter, even if taken from the same spore print). Its very difficult to nail down any specifics for a strain other than your own personal collection. Now, a few cases do exist of course, such as the wonkyness of PE and its relatives, the red spored varieties, etc, but by and large it will be a crap shoot.

I know that doesn't really help you much, but its about the best I have to offer.




Yea I totally agree. Aside from the apparent slow colonization, sometimes strains can have other traits that compensate for it. So don't feel as if that is a negative thing. It's more just a fact of nature and odds in general.

I was actually curious about that strain as well. Did you find that your isolate had other good traits? I heard it is good in the potency department. :wink:

What are your thoughts on the strain so far?


--------------------
Cheers,

bzl

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------
"From 1898 through to 1910 heroin was marketed as a non-addictive morphine substitute and cough medicine for children."

conclusion:
poor fucking children of the early 1900's.


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Offlineknomadic_niki
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Re: Isolating multispore cultures [Re: bryanbzl]
    #21902100 - 07/05/15 05:50 PM (8 years, 6 months ago)

all i've gotten so far is that it is strong and rhizomorphic on agar, slightly slow on coco coir, and smells nice and earthy. it was just introduced into the fruiting chamber today


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