|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Sharpstuff


Registered: 05/04/11
Posts: 510
Loc: Australia
Last seen: 7 years, 7 months
|
Salinity and Mycelium
#21899666 - 07/05/15 04:19 AM (8 years, 6 months ago) |
|
|
Has anybody worked with using fungi with salty water (similar to what stamets has done with the mycoboom). I am interested to know how the fungi deal with the ions, particularly sodium chloride. This patent here > http://patents.justia.com/patent/20140097008 indicates that "Fungal species have developed multiple pathways to inactivate free radicals and accumulate the ion species either within the cell (chelation) or on the exterior of the cell wall (biosorption)."
Stamets also talks of a this process being used in the hyper-accumulation of radioactive isotopes in the fruiting bodies. Do you guys think the same could be done for salt ions such as sodium chloride?
I've read several things on the shroomery in regards to salinity but have not have much of a conclusive answer.
Interested to discuss this further!
|
Chk
Reverser


Registered: 09/06/13
Posts: 405
Loc: France
Last seen: 5 years, 3 months
|
Re: Salinity and Mycelium [Re: Sharpstuff]
#21900633 - 07/05/15 11:43 AM (8 years, 6 months ago) |
|
|
I tested several jars with grounded bread as a replacement for brf (as a way to recycle it) Bread contain 15g salt per kilogram (where i live it's the mean value more or less)
Colonization was very slow, yield was bad.
Also i tested a very contaminated LC on that substrate, and it seems bacteria didn't like it XD
Imo even if it prevents bacteria from colonizing, it's not worth it.
Still it would be interesting to make tests with increasing levels of salt, to see how much you can add before it prevents growth, i bet it's quite a lot
Edited by Chk (07/05/15 11:45 AM)
|
Sharpstuff


Registered: 05/04/11
Posts: 510
Loc: Australia
Last seen: 7 years, 7 months
|
Re: Salinity and Mycelium [Re: Chk]
#21903211 - 07/05/15 10:43 PM (8 years, 6 months ago) |
|
|
What species did you grow? I wasn't looking at doing it to prevent contamination I work with a flow hood so I don't have those issues 
I was thinking more of long the lines of fungi removing (chellating) the salt from the water resulting in fresh water to some degree. Bit beyond simple cultivation hence why I posted in Advanced Mycology!
|
forrest



Registered: 11/16/12
Posts: 1,011
Loc: The Netherlands
Last seen: 4 years, 6 months
|
Re: Salinity and Mycelium [Re: Sharpstuff]
#21903752 - 07/06/15 01:13 AM (8 years, 6 months ago) |
|
|
i used some vegemite in my agar and the myc grew fine. But i only added like a gram or two in 500 ml
-------------------- My Trade List
|
Toadstool5
A Registered Mycophile



Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
|
Re: Salinity and Mycelium [Re: forrest]
#21905895 - 07/06/15 04:09 PM (8 years, 6 months ago) |
|
|
Read:
Biosorption of malachite green from aqueous solutions by Pleurotus ostreatus using Taguchi method
Zhengsuo Chen, Hongbo Deng, Can Chen, Ying Yang and Heng Xu*
I might be able to email you the pdf if its not too large, its also on google. me if you cant find it 
Quote:
Effect of ionic strength
There are various impurities, which could be acids, alkalis, salts or metal ions in the wastewaters from textile-manufacturing or dye-producing industries. The presence of cations such as Na+, K+, Cu2+, Ca2+ and Cr3+, which are the most common metal ions existing in dye wastewater that may affect the performance of the biosorption process.
Figure 4 shows the effect of monovalent Na+ and divalent Ca2+ on the removal percentage of dye. It was observed that biosorptive capacity for biosorbent decreased with increasing in the concentration of Na+ and Ca2+. As concentration of Na+ changed from 0.05 M to 0.2 M, the percent removal of MG changed from 87.22% to 49.01%, with a decreased of 38.21%. As concentration of Ca2+ changed from 0.05 M to 0.2 M, the percent removal of MG changed from 83.73% to 43.57%, with a decreased of 40.16%. From 0.05 to 0.2 M, all the concentration, divalent ions Ca2+ had a greater inhibiition than monovalent ions Na+ on the percent removal, which ranged from 3.49% to 5.44%. With increasing in the concentration, the gap was bigger. It was an adverse effect of ionic strength on MG removal. It may be owed to the possibility of ion exchange mechanisms in the biosorption process. The competition exist between Na+, Ca2+ and positively charged MG molecules for the same binding sites on the biosorbent surface. Ca2+ had greater positive charge than Na+ and had a stronger competitive ability. Similar observations were previously reported in aqueous solution for removal of chromium (VI) by Dunaliella species [35] and dye removal by Poly (propylene imine) Dendrimer [36]
The functional groups responsible for binding are O-H, N-H, and methyl/methylene.
Preparation of the biosorbant
Quote:
The macro-fungus P. ostreatus was purchased from a mushroom production site near Chengdu, Sichuan. The samples were repeatedly cleaned with deionized water to remove adhering dirt and soluble impurities, and boiled with distilled water three times, each for 3–5 minutes filter with the distilled water until the filtered water was cleared.
They were dried at 50°C until keep constant weight, then crushed and sieved through 200 mesh (< 75 μm) sieve to get the smaller particles. The sieved biosorbent were kept in a plastic sealable bag and stored in a desiccator without any further chemical or physical treatment before adsorption experiments
Whats interesting is that it worked WITHOUT dielectrical polarization. If you were to conduct the "filtration" on an electrically charged media like mentioned in the study you linked then the adsorption of the sodium ions would increase even more!
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
|
rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,530
Loc: FREE PSYCHONAUTICA
Last seen: 2 minutes, 46 seconds
|
Re: Salinity and Mycelium [Re: Toadstool5]
#21907117 - 07/06/15 08:50 PM (8 years, 6 months ago) |
|
|
seems like bread could also contain a number of things other than salt that migh inhibit growth.
why not do a test with agar, and several different salt concentrations and several different types of fungi and see which ones perform the best.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
|
Toadstool5
A Registered Mycophile



Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
|
Re: Salinity and Mycelium [Re: rxb]
#21907683 - 07/06/15 10:40 PM (8 years, 6 months ago) |
|
|
Quote:
several different types of fungi and see which ones perform the best.
Definitely your best course of action! I believe i saw a paper with similar data with several different species. I will try to dig it up for you. Its pretty fucked up calculus though so i dont completely understand the variables and coefficient.
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
|
Toadstool5
A Registered Mycophile



Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
|
Re: Salinity and Mycelium [Re: Toadstool5]
#21907710 - 07/06/15 10:53 PM (8 years, 6 months ago) |
|
|
This study lists some of the methods for quantitatively measuring adsorption of certain salts in non-edible species. Could essentialy be reconfigured with sodium sulfate instead of copper sulfate and whatever species you are best suited to produce.
Quote:
Screening of Tropical Wood-Rotting Mushrooms for Copper Biosorption T. R. MURALEEDHARAN,1 LEELA IYENGAR,2 * AND C. VENKOBACHAR3 Indira Gandhi Institute of Development Research, Bombay, Goregaon (E) 400 065,1 Department of Chemistry,2 and Environmental Engineering, Department of Civil Engineering,3 Indian Institute of Technology, Kanpur, Kanpur 208 016, India
Received 27 January 1995/Accepted 4 May 1995
Fruiting bodies (mushrooms) of nine nonedible macrofungi were screened for copper(II) uptake potential. The maximum uptake potentials (Qmaxs) derived from equilibrium studies indicated that all nine species exhibited higher Qmaxs at pH 4.0 than that of Filtrasorb-400, a generally used adsorbent for metal removal.
Wide variation in Qmax was observed among the species and ranged from 0.048 to 0.383 mmol per g of sorbent. The uptake capacity of Ganoderma lucidum, which exhibited the highest Qmax, was higher than those of other microbial biosorbents reported in the literature.
The Langmuir relationship is the formula for evaluating a biosorbents' efficiency
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
|
Sharpstuff


Registered: 05/04/11
Posts: 510
Loc: Australia
Last seen: 7 years, 7 months
|
Re: Salinity and Mycelium [Re: Toadstool5]
#21992708 - 07/24/15 09:35 PM (8 years, 6 months ago) |
|
|
Some information in relation to this thread
Hey guys,
interested to hear your thoughts on this very interesting information regarding fungus species that can 'lock up' gold and other metals with proteins making them non-toxic to the fungus.
http://fraden.brandeis.edu/publications/papers/Gorman%20Science%20News%202003.pdf
I wonder if a fungi species could 'lock up' salt ions similar to what is being done in the above study. I posted awhile ago about Geoff Lawton in his 'greening the desert' video and how he mentioned fungi made the salt inert making crops able to grow in otherwise salty soil.
|
|