|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,530
Loc: FREE PSYCHONAUTICA
Last seen: 2 minutes, 38 seconds
|
Re: rxb agar jar tek - low cost low tek isolation solution (moved) [Re: Mr.GuessWork]
#21937358 - 07/13/15 01:36 PM (8 years, 6 months ago) |
|
|
i mix spores with water.
i drip spore syringes in multiple spots.
also it has this neat feature where if you put a quarter cc from a spore syringe on the agar... (enough to get a good bead of water)... you can then roll the bead around.... over the whole plate... then turn it upside down for a day or two (so the bead doesnt sit on the agar)... and the whole plate gets COVERED In colonies.
so your techniques just adapt to the situation.
but you know, if its not your idea of a good time, you dont need to try it 
but i think if you do, you'll be pleasantly surprised at how simple it is to do whatever you'd like
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
|
spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
Re: rxb agar jar tek - low cost low tek isolation solution (moved) [Re: rxb]
#21937383 - 07/13/15 01:42 PM (8 years, 6 months ago) |
|
|
how do you get myc out of the agar plate if you dont use a SAB?
|
rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,530
Loc: FREE PSYCHONAUTICA
Last seen: 2 minutes, 38 seconds
|
Re: rxb agar jar tek - low cost low tek isolation solution (moved) [Re: rxb]
#21937386 - 07/13/15 01:42 PM (8 years, 6 months ago) |
|
|
forgive the horrible picture
this was done thursday or friday night with that roll the bead around technique.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
Edited by rxb (07/13/15 01:42 PM)
|
rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,530
Loc: FREE PSYCHONAUTICA
Last seen: 2 minutes, 38 seconds
|
Re: rxb agar jar tek - low cost low tek isolation solution (moved) [Re: spacechildo]
#21937395 - 07/13/15 01:44 PM (8 years, 6 months ago) |
|
|
Quote:
spacechildo said: how do you get myc out of the agar plate if you dont use a SAB?
from the tek
Quote:
16) because we are using foil and a very short jar, a specific sector can be isolated by putting the needle into the jar right above the sector. Make a small circle with the needle around the sector to be sampled as a boundary layer. And scratch a grid across the top of the sector
17) be sure you are on a level work surface and put a few drops of water in the sector and stir the water into the agar then add another drop or two. Then pull the water back into the syringe and repeat the process until you are satisfied you have enough material.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
|
spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
Re: rxb agar jar tek - low cost low tek isolation solution (moved) [Re: rxb]
#21937405 - 07/13/15 01:46 PM (8 years, 6 months ago) |
|
|
have you transfered away from contams successfully like that?
|
rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,530
Loc: FREE PSYCHONAUTICA
Last seen: 2 minutes, 38 seconds
|
Re: rxb agar jar tek - low cost low tek isolation solution (moved) [Re: spacechildo]
#21937408 - 07/13/15 01:47 PM (8 years, 6 months ago) |
|
|
Quote:
spacechildo said: have you transfered away from contams successfully like that?
nope, i havent contaminated one yet. - i will contaminate one on purpose, i have a liquid culture with green mold, i just have to do it and have not yet
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
Edited by rxb (07/13/15 01:48 PM)
|
spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
Re: rxb agar jar tek - low cost low tek isolation solution (moved) [Re: rxb]
#21937421 - 07/13/15 01:50 PM (8 years, 6 months ago) |
|
|
it sounds real risky to me. just cant see it being a feasable way of dealing with agar transfers..
|
rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,530
Loc: FREE PSYCHONAUTICA
Last seen: 2 minutes, 38 seconds
|
Re: rxb agar jar tek - low cost low tek isolation solution (moved) [Re: spacechildo]
#21937426 - 07/13/15 01:51 PM (8 years, 6 months ago) |
|
|
Quote:
spacechildo said: how do you get myc out of the agar plate if you dont use a SAB?
Quote:
spacechildo said: it sounds real risky to me. just cant see it being a feasable way of dealing with agar transfers..
some people have to try things before they understand how they work.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
|
rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,530
Loc: FREE PSYCHONAUTICA
Last seen: 2 minutes, 38 seconds
|
Re: rxb agar jar tek - low cost low tek isolation solution (moved) [Re: rxb]
#21937430 - 07/13/15 01:53 PM (8 years, 6 months ago) |
|
|
im not sure exactly what you are risking?
the 2 drops from a spore syringe?
the 10 bux or less it costs to make?
the 10 minutes it takes to make one?
or just your illusions that you cant work without a flow hood?
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
|
spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
Re: rxb agar jar tek - low cost low tek isolation solution (moved) [Re: rxb]
#21937434 - 07/13/15 01:53 PM (8 years, 6 months ago) |
|
|
exactly!
report back once you've tried transfering away from contams
|
rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,530
Loc: FREE PSYCHONAUTICA
Last seen: 2 minutes, 38 seconds
|
Re: rxb agar jar tek - low cost low tek isolation solution (moved) [Re: spacechildo]
#21937437 - 07/13/15 01:54 PM (8 years, 6 months ago) |
|
|
I SURE WILL!
you do the same!
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
|
rxb
n00b-sabot



Registered: 08/24/13
Posts: 9,530
Loc: FREE PSYCHONAUTICA
Last seen: 2 minutes, 38 seconds
|
Re: rxb agar jar tek - low cost low tek isolation solution (moved) [Re: rxb]
#21937462 - 07/13/15 02:02 PM (8 years, 6 months ago) |
|
|
part of the reason i havent done this yet, is that the above photo looks TERRIBLE. i have good camera equipment and i need to get it out and set things up and take GOOD photos.
so i've done a lot of peat and repeat.
but i'd like to be more show and tell.
i'll knock one up with mold this week...and i'll try to get the real camera setup.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <- . i cleaned a mold contaminated live culture and saved it. (might have useful applications) [quote]Enlil said: I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
|
Mr.GuessWork
Stranger

Registered: 03/30/13
Posts: 4,563
|
Re: rxb agar jar tek - low cost low tek isolation solution (moved) [Re: rxb]
#21937491 - 07/13/15 02:11 PM (8 years, 6 months ago) |
|
|
Quote:
rxb said: i mix spores with water.
i drip spore syringes in multiple spots.
also it has this neat feature where if you put a quarter cc from a spore syringe on the agar... (enough to get a good bead of water)... you can then roll the bead around.... over the whole plate... then turn it upside down for a day or two (so the bead doesnt sit on the agar)... and the whole plate gets COVERED In colonies.
so your techniques just adapt to the situation.
but you know, if its not your idea of a good time, you dont need to try it 
but i think if you do, you'll be pleasantly surprised at how simple it is to do whatever you'd like 
It's definitely got some advantages. It probably doesn't waste as much of the agar as a 3 or 4 streak method does. I think I'll try something like it next time I play with agar. Thanks for the inoculation specifics.
|
|