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jesuisravi
The Old Noob


Registered: 06/24/15
Posts: 260
Loc: Midwest USA
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an oyster experiment
#21899022 - 07/04/15 10:55 PM (8 years, 6 months ago) |
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I have never grown anything but Cubenses. I thought I would make a tiny stab at something else. I took two 4 oz wide mouth kerr jars, filled them with PF tek BRF and vermiculite. Pressure cooked them. Then I took an 8 oz wide mouth Kerr jar, poured it half full of water, screwed the cutting end of an Osterizer on it and pressure cooked that. Then I took an oyster mushroom I had bought the previous day at the market, cut the stem from the cap, wiped down the stem well with 91% alcohol--all this done in a still air box--, unscrewed the osterizer blades from the jar of water,quickly dropped the piece of oyster stem into the sterilized water, and screwed the blades back on. Then I Osterized the stem for a few seconds until it was pretty well shredded but not atomized. I went back to the SAB, opened quick quick the two BRF jars and doled out a teaspoonful of the osterized oyster flesh onto the top of each jar. All this was done as sterilely as possible with alcohol and flame sterilized impliments. I wonder if anyone would care to comment on what my chances are for successfully fruiting the two jars?
-------------------- Most of my beliefs I acquired from my father and from John Wayne, and anything that wasn't ultra tough and ultra cool was to me ultra embarrassing. In fact, I lived in a state of near continuous embarrassment, never measuring up to the ridiculous standards I had accepted without question, applied to a framework of expectations neither I nor anyone else could meet.--J C Amberchele almost nothing important that ever happens to you happens because you engineer it. Destiny has no beeper; destiny always leans trenchcoated out of an alley with some sort of 'psst' that you usually can't even hear because you're in such a rush to or from something important you've tried to engineer. ” ― David Foster Wallace, Infinite Jest
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Toadstool5
A Registered Mycophile



Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
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Re: an oyster experiment [Re: jesuisravi]
#21899075 - 07/04/15 11:17 PM (8 years, 6 months ago) |
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Did you use a dry verm layer in the jars? That wouldnt help your odds of success.
It should work if cross contams dont set in and the mycelial slushie is able to re-establish and colonize the substrate. You didnt completely obliterate the mycelium so i think it would colonize but i would have removed the mycelial filament from the stem rather than macerating the entire stem
Fruiting oysters takes a lot of humidity compared to cubes. They prefer bags, tubing, baskets, and buckets compared to trays or a SGFC.
Welcome to the dark side!! We have cookies
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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jesuisravi
The Old Noob


Registered: 06/24/15
Posts: 260
Loc: Midwest USA
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Re: an oyster experiment [Re: Toadstool5]
#21899723 - 07/05/15 05:03 AM (8 years, 6 months ago) |
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I used a layer of dry verm in one and no dry layer in the other--just to see what difference it would make.
Well, we shall see. I will post pics if anything develops.
Edited by jesuisravi (07/05/15 05:05 AM)
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jesuisravi
The Old Noob



Registered: 06/24/15
Posts: 260
Loc: Midwest USA
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Re: an oyster experiment [Re: jesuisravi]
#21936353 - 07/13/15 08:17 AM (8 years, 6 months ago) |
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It has been 9 days since I inoculated to 4 oz PF jars with mycelium shredded from a store bought oyster mushroom. One of the jars is showing the unmistakable signs--or sign--that myc kcolonization is beginning. I will pst a pic later today.
-------------------- Most of my beliefs I acquired from my father and from John Wayne, and anything that wasn't ultra tough and ultra cool was to me ultra embarrassing. In fact, I lived in a state of near continuous embarrassment, never measuring up to the ridiculous standards I had accepted without question, applied to a framework of expectations neither I nor anyone else could meet.--J C Amberchele almost nothing important that ever happens to you happens because you engineer it. Destiny has no beeper; destiny always leans trenchcoated out of an alley with some sort of 'psst' that you usually can't even hear because you're in such a rush to or from something important you've tried to engineer. ” ― David Foster Wallace, Infinite Jest
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