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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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uncle_rico
my own worst enemy


Registered: 03/28/06
Posts: 2,664
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Re: Post Your Cultivation Picture of The Day [Re: Aero]
#21897410 - 07/04/15 04:29 PM (8 years, 10 months ago) |
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Life Cycle in a Petri Dish
or, what happens when you forget about an aggressive culture ....
kernel of grain on agar and forgotten

more time passes


combination clone and spore print!
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BlazinAmazin
Dab King



Registered: 06/18/15
Posts: 45
Last seen: 1 year, 2 months
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Re: Post Your Cultivation Picture of The Day [Re: uncle_rico]
#21897561 - 07/04/15 04:59 PM (8 years, 10 months ago) |
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That is awesome!
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Post Your Cultivation Picture of The Day [Re: uncle_rico]
#21897834 - 07/04/15 05:48 PM (8 years, 10 months ago) |
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Quote:
uncle_rico said: Life Cycle in a Petri Dish
or, what happens when you forget about an aggressive culture ....
kernel of grain on agar and forgotten

more time passes


combination clone and spore print!
I've been starting all my grows with petri clones for a while. It's a great way to get clean spores too.

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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 4 days, 18 hours
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Re: Post Your Cultivation Picture of The Day [Re: MudaFuka]
#21898709 - 07/04/15 09:46 PM (8 years, 10 months ago) |
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Quote:
MudaFuka said:
Quote:
uncle_rico said: Life Cycle in a Petri Dish
or, what happens when you forget about an aggressive culture ....
kernel of grain on agar and forgotten

more time passes


combination clone and spore print!
I've been starting all my grows with petri clones for a while. It's a great way to get clean spores too.
 
Why are you putting whole fruits on the plate? I understand just dropping a tiny pin, but a whole fruit? I dont want spores tainting my clone !
Just curious!
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 Amateur Mycologists United AMU Q&A
Edited by mushpunx (07/04/15 09:56 PM)
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ShroominMe
Stranger

Registered: 05/03/15
Posts: 525
Last seen: 8 years, 6 months
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Re: Post Your Cultivation Picture of The Day [Re: mushpunx]
#21898742 - 07/04/15 09:53 PM (8 years, 10 months ago) |
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Quote:
mushpunx said:
Why are you putting whole fruits on the plate? I understand just dropping a tiny pin, but a whole fruit? I dont want spores tainting my clone !
Not to mention whatever is lurking on the fruit body. I tear the fruit and biopsy a tiny piece of virgin tissue to drop
Just curious!
Did you read his post? He said:
Quote:
kernel of grain on agar
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grownright
Duke of Lizzards



Registered: 10/06/14
Posts: 1,030
Loc: Deep South
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Re: Post Your Cultivation Picture of The Day [Re: mushpunx]
#21898744 - 07/04/15 09:53 PM (8 years, 10 months ago) |
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Quote:
mushpunx said: Why are you putting whole fruits on the plate? I understand just dropping a tiny pin, but a whole fruit? I dont want spores tainting my clone !
Not to mention whatever is lurking on the fruit body. I tear the fruit and biopsy a tiny piece of virgin tissue to drop
Just curious!
no, hes saying you leave an isolate in a dish and leave the mycelium to fully colonize until it pins in the dish, then you clone the pin. It allows for a much cleaner specimen to clone from and you know youll be isolating an aggressive, fruiting strain.
I actually have an isolate right now that I started from an invitro pin that had grown on my first brf paste jar. I wanted to practice before trying agar, but had forgotten about the jar. I found a pin in it one day and I had poured some agar that morning so I cloned it. I cant wait to see how it turns out
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Edited by grownright (07/04/15 09:56 PM)
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 4 days, 18 hours
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Re: Post Your Cultivation Picture of The Day [Re: blindingleaf]
#21898753 - 07/04/15 09:54 PM (8 years, 10 months ago) |
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Quote:
blindingleaf said:

do u do golf courses too?
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 Amateur Mycologists United AMU Q&A
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 4 days, 18 hours
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Re: Post Your Cultivation Picture of The Day [Re: grownright]
#21898771 - 07/04/15 09:58 PM (8 years, 10 months ago) |
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Quote:
grownright said:
Quote:
mushpunx said: Why are you putting whole fruits on the plate? I understand just dropping a tiny pin, but a whole fruit? I dont want spores tainting my clone !
Not to mention whatever is lurking on the fruit body. I tear the fruit and biopsy a tiny piece of virgin tissue to drop
Just curious!
no, hes saying you leave an isolate in a dish and leave the mycelium to fully colonize until it pins in the dish, then you clone the pin. It allows for a much cleaner specimen to clone from and you know youll be isolating a fruiting strain.
Haha no I didnt see it was Muda's photo and I didnt really read it Im a little loopy hah
I just looked at the picture and was like "did he just drop a whole fruit on a plate and let it grow" ? I was like how are you gunna get clean growth off of that
Didnt realize those were pitres left to fruit thats embrrasing
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 Amateur Mycologists United AMU Q&A
Edited by mushpunx (07/04/15 10:02 PM)
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Post Your Cultivation Picture of The Day [Re: mushpunx]
#21898774 - 07/04/15 09:59 PM (8 years, 10 months ago) |
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Those fruits grew on the plates those are in-vitro pins that I left to mature and drop spores. I always weight for a pin to form on my agar before starting a grow with a new variety. It lets me get a clone without going threw a whole grow and in-vitro petri pins are almost always really strong fruiters and fast pinners.
 hear are a few more
  enoki
 lions mane
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 4 days, 18 hours
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Re: Post Your Cultivation Picture of The Day [Re: MudaFuka]
#21898791 - 07/04/15 10:05 PM (8 years, 10 months ago) |
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Quote:
MudaFuka said: Those fruits grew on the plates those are in-vitro pins that I left to mature and drop spores. I always weight for a pin to form on my agar before starting a grow with a new variety. It lets me get a clone without going threw a whole grow and in-vitro petri pins are almost always really strong fruiters and fast pinners.
 hear are a few more
  enoki
 lions mane
Yea dude I gotcha. I grab clones like this too sometimes
I looked at the photo and thought it was a whole fruit dropped onto a plate it 4th of july and Im feeling loopy
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 Amateur Mycologists United AMU Q&A
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Post Your Cultivation Picture of The Day [Re: mushpunx]
#21898813 - 07/04/15 10:09 PM (8 years, 10 months ago) |
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liquid culture from 2007.
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Buck513

Registered: 04/17/14
Posts: 5,682
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Re: Post Your Cultivation Picture of The Day [Re: eatyualive]
#21898824 - 07/04/15 10:13 PM (8 years, 10 months ago) |
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How often will a plate produce a pin? Should you add more agar since it will be sitting around for a long time?
-------------------- Fail to plan and you plan to fail. Enter the Ban Lottery
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Post Your Cultivation Picture of The Day [Re: Buck513]
#21898836 - 07/04/15 10:15 PM (8 years, 10 months ago) |
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:O eat! That looks delicious!
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Post Your Cultivation Picture of The Day [Re: Buck513]
#21898864 - 07/04/15 10:22 PM (8 years, 10 months ago) |
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It depends on genetics. I've had some dishes pin just a couple days after the mycelium hits the side of the dish. Others like PE take several weeks. I don't add any extra agar. It doesn't dry out as fast as you might think. I've got plates sitting at room temperature that are almost a year old and they still aren't completely dried out. Some of them have put out multiple flushes. Lol
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Post Your Cultivation Picture of The Day [Re: MudaFuka]
#21898912 - 07/04/15 10:33 PM (8 years, 10 months ago) |
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i had a tex yellow cap pin before the plate was fully colonized. the ape pins fast as well on a plate. but slow as shit on subs for me.
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Post Your Cultivation Picture of The Day [Re: eatyualive]
#21898942 - 07/04/15 10:40 PM (8 years, 10 months ago) |
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Jon Allens pin the fastest for me on agar. KSSS and PE6 are close seconds.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 4 days, 18 hours
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Re: Post Your Cultivation Picture of The Day [Re: MudaFuka]
#21899005 - 07/04/15 10:53 PM (8 years, 10 months ago) |
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Quote:
MudaFuka said: It depends on genetics. I've had some dishes pin just a couple days after the mycelium hits the side of the dish. Others like PE take several weeks. I don't add any extra agar. It doesn't dry out as fast as you might think. I've got plates sitting at room temperature that are almost a year old and they still aren't completely dried out. Some of them have put out multiple flushes. Lol
You leave them wrapped tho yea?
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 Amateur Mycologists United AMU Q&A
Edited by mushpunx (07/04/15 11:05 PM)
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Post Your Cultivation Picture of The Day [Re: mushpunx]
#21899037 - 07/04/15 10:59 PM (8 years, 10 months ago) |
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yeah.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 4 days, 18 hours
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Re: Post Your Cultivation Picture of The Day [Re: MudaFuka]
#21899059 - 07/04/15 11:08 PM (8 years, 10 months ago) |
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Ive never had much sucess getting plates to pin. When they have in the past Ive cloned them with good results.
Ill let plates grow out amd leave them arpund for ages w out pin Any tips/tricks dude?
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 Amateur Mycologists United AMU Q&A
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,866
Loc: Canada
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Re: Post Your Cultivation Picture of The Day [Re: mushpunx]
#21899108 - 07/04/15 11:31 PM (8 years, 10 months ago) |
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Extra nutes can help a bit with pinning. More starch less sugar also helps. MEA pins poor, grain water, potato starch and dog food seem to do better.
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