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OfflinePutACapInHisAss
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Is There a Flame Sterilization Tek for Still Air Boxes?
    #21897620 - 07/04/15 05:11 PM (8 years, 6 months ago)

I know this seems super simple, but when using a still air box, there must be a proper way. I see that most people do it outside of the box to avoid creating air currents or lighting up alcohol based cleaners used inside.

If you are inoculating multiple containers, do you flame outside of the box between each one, every other one, once per session...? What else to know? Is it possible to damage the needle or the contents? Is a lighter good enough or must I have an alcohol lamp? Wouldn't bringing it in and out of a still air box many times create vacuums moving around air in and out of the box, and possibly getting spores on the surface of the needle not flamed?


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OfflineSyntheticwords
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: PutACapInHisAss]
    #21897811 - 07/04/15 05:44 PM (8 years, 6 months ago)

I always flame my needle, scalpel, loop whatever I use outside the box

I used to use a lighter in the box and never had much worries aside from my thumb catching on fire from using ISO alchol on the sterilization port
Just make sure you don't spray a whole bunch of Lysol and light up the flame though...


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InvisibleToadstool5
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: PutACapInHisAss]
    #21897864 - 07/04/15 05:56 PM (8 years, 6 months ago)

A lighter works but is not as hot so it takes longer, they are also a pain to use with gloves on. I personally use a propane torch for gourmet when i do pf-tek (no difference)

Flame between every jar but not between holes.

When you take it out, move very slowly and always wear a surgical mask (bouffant caps help too) then move the flamed needle back into the SAB while it is still red hot so anything that lands on it will die.

When flaming be careful to not melt the luer lock on the syringe and be careful with red hot needles. When they are hot they become really soft and are easy to bend by mistake.

Never light up inside the SAB, its not necessary or safe.

If you are really worried about air currents: clean the entire room with 3% bleach (allow it to sit for 10min), allow the room to settle by turning off the ac and fans for one hour, run a clean HEPA filter for awhile, turn it off and spray lysol everywhere right before you work. Remember to wear a surgical mask and use a shower cap or bouffant cap for dander and tyvek sleeves to help prevent dander and air currents from making their way through the SAB holes.

You will figure it out! We all stress how important sterility is but that doesnt mean its difficult, you only need vigilance and a deep cleaning :thumbup:


--------------------
If you do not know where the mushroom products you are consuming are grown, think twice before eating them. :badshroom:
- Paul Stamets

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InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Toadstool5]
    #21898184 - 07/04/15 07:28 PM (8 years, 6 months ago)

A lighter can be used, but its inferior to butane torches and stuff like that. But if its all you got, its all you got:shrug:

Never sterilize inside SAB, also remember its good to let the air settle every time you bring your hands back in the SAB for a good 20-30 seconds. That time is also used so the tools can have a chance to cool down a bit.


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OfflineMachiavelliavore
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: PutACapInHisAss]
    #21898246 - 07/04/15 07:48 PM (8 years, 6 months ago)

I've tried a few techniques.  In the past I just said fuck it and used the lighter in the box, because I'd rather stir up what inside it than bring in air current from outside that haven't been bleach nuked by taking my arms in and out over and over.

If you're doing lid pops, I now prepare all the lids (get them very lose/remove rings,) then flame my needle outside, and go from jar to jar, squirt squirt.  If I have to re-arrange shit in the airbox to do another set, I'll reflame.
IMO:
a) Any contams that land on the tiny surface area of the syringe needle will ABSOLUTELY fall out of the air into the containers when the lids are opened, and in far greater concentration.  On top of that, only the very tip of the needle would transfer these contams into the jars.

b) Any contams in the syringe are unaffected by flaming.

c) If you're using ports/poly, flame every time, since you are rubbing the needle against shit and it's literally the only thing entering the jar.  Flame in the air box (after any more sensitive work is completed) if you bother to use one.

Just try to be as fast and smooth as possible if opening lids, and try to keep your hands from being above the open container.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Machiavelliavore]
    #21898262 - 07/04/15 07:52 PM (8 years, 6 months ago)

Please don't advise someone to flame sterilize inside a SAB.
Quote:

Machiavelliavore said:
In the past I just said fuck it



That type of attitude will get you literally nowhere in this hobby.


--------------------
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OfflineMachiavelliavore
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Buck513]
    #21898312 - 07/04/15 08:06 PM (8 years, 6 months ago)

Do you have some scientific studies that say using a lighter in the a confined space the size of a glovebox creates more turbulance than moving your arms in and out?

I have sleeves on my air box, so my turbulance is increased going in and out, and the interaction with outside air due to internal turbulance is decreased, so I would rather flame inside my air box than go in and out constantly.  If I only need to flame something once (say a needle tip) now I will do it outside rather than flaming it between every jar (which as I outlined, I believe to be illogical when popping lids.)

Certainly the air spore concentration inside the glove box is considerably less inside the air box than outside, therefore I would rather create some turbulance in the internal air than exchange a significant amount of air with the outside.

I said "fuck it" because both options are rather shitty in my opinion.  I've had very few trich/mold problems.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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InvisibleBuck513

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Posts: 5,682
Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Machiavelliavore]
    #21898323 - 07/04/15 08:09 PM (8 years, 6 months ago)

Quote:

Machiavelliavore said:
Do you have some scientific studies that say using a lighter in the a confined space the size of a glovebox creates more turbulance than moving your arms in and out?

I have sleeves on my air box, so my turbulance is increased going in and out, and the interaction with outside air due to internal turbulance is decreased, so I would rather flame inside my air box than go in and out constantly.  If I only need to flame something once (say a needle tip) now I will do it outside rather than flaming it between every jar (which as I outlined, I believe to be illogical when popping lids.)

Certainly the air spore concentration inside the glove box is considerably less inside the air box than outside, therefore I would rather create some turbulance in the internal air than exchange a significant amount of air with the outside.

I said "fuck it" because both options are rather shitty in my opinion.  I've had very few trich/mold problems.



:doublefacepalm:


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InvisibleInocuole
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Buck513]
    #21898344 - 07/04/15 08:15 PM (8 years, 6 months ago)

Not only does that create air currents, but some people spray the inside of the SABs with isopropyl and still more people aren't the smartest in their pay grade, so naturally they will blow all the hair off their arms and maybe their face taking that advice.  I flame outside the SAB and it's fantastic.


--------------------
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InvisibleMad Season
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Inocuole]
    #21898377 - 07/04/15 08:24 PM (8 years, 6 months ago)

:whathesaid: even a qt sized grain jar can cause too much air movement during g2gs. That's why you need a big box to reduce the amount of air movement.

You need clean air to burn a flame. Heat rises too. It's no different than having a fire in your living room. Principles still apply.


--------------------
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Edited by Mad Season (07/04/15 08:26 PM)


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InvisibleInocuole
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Mad Season]
    #21898391 - 07/04/15 08:28 PM (8 years, 6 months ago)

Quote:

Mad Season said:
:whathesaid: even a qt sized grain jar can cause too much air movement during g2gs. That's why you need a big box to reduce the amount of relative air movement




Edited for science.


--------------------
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InvisibleMad Season
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Inocuole]
    #21898394 - 07/04/15 08:29 PM (8 years, 6 months ago)

LOL. :ohyou:


--------------------
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InvisibleBuck513

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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Mad Season]
    #21898447 - 07/04/15 08:45 PM (8 years, 6 months ago)

Science:ancientaliens:


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OfflineMachiavelliavore
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Inocuole]
    #21898504 - 07/04/15 09:01 PM (8 years, 6 months ago)

Obviously you shouldn't light up in an air box covered with flammables.


If many repetitive flamings are required, it's nice not to have to clean gloves and sleeves over and over due to outside air exposure.  I'm not saying it's always right to flame inside, I'm saying there are trade offs to either option, especially if you have sleeves which make it harder to come in and out of your airbox, or you forgot about that moldy tomato 10 feet away :smile:  Sometimes I'd rather create turbulence in a cleaner environment than venture into a dirty one to spare it.

Again, I use plastic bags around my arms that extend off the holes, so I'm a bit more isolated from the outside that a standard 2 holes n nothin' type box.

I've had plenty of success with direct clone tissue samples onto grain petris while flaming an alcohol covered knife in my box.  It has been my experience that most contams come from bad inoculation sources or endospores.

Glad to see how you got to 3.5k posts in a year buck  :projectile:


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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InvisibleMad Season
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Machiavelliavore]
    #21898518 - 07/04/15 09:05 PM (8 years, 6 months ago)

You probably actually wouldn't want to see all his posts LOLOL.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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InvisibleBuck513

Registered: 04/17/14
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Machiavelliavore]
    #21898530 - 07/04/15 09:08 PM (8 years, 6 months ago)

How many tubs have you grown?
What teks do you follow? Or are you such a myco guru you don't follow teks?

Go look at my posts if you want fool. I don't know what you mean by "I see how you got 3.5k posts".


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InvisibleBuck513

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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Mad Season]
    #21898540 - 07/04/15 09:11 PM (8 years, 6 months ago)

Quote:

Mad Season said:
You probably actually wouldn't want to see all his posts LOLOL.



Probably not. I tend to be rather blunt and I come off as condescending sometimes. But I'm really not. I like laughing at people though:shrug:
And people laugh at me. Who gives a shit. Its a drug forum. I'm glad to offer up the small bit of knowledge I have, I'm even more glad o learn something I didn't already know.
Too many over sensitive people are around these days.


--------------------
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OfflineInextinctus
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Machiavelliavore]
    #21898561 - 07/04/15 09:15 PM (8 years, 6 months ago)

Curious to know, with the whole outside/inside air transfers... Wouldn't it make more sense to actually attach your arm covers, whatever they may be, to your box so that there's an air tight seal and you can slide your hands out with ease? No sleeves attached?
Keep flammable substances out of the box and boom? (No pun intended)


--------------------
Soundcloud.com/inextinctus
what are we but a different combination of the elements we consume?
"So then I :lsd:"



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InvisibleMad Season
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Buck513]
    #21898567 - 07/04/15 09:17 PM (8 years, 6 months ago)

Word buck. Definitely best place to work on this hobby :smile:. Just thinking about the granny tits quoted from earlier. :sick: haha


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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InvisibleBuck513

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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Mad Season]
    #21898583 - 07/04/15 09:22 PM (8 years, 6 months ago)

.


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Edited by Buck513 (07/04/15 09:25 PM)


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InvisibleMad Season
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Buck513]
    #21898599 - 07/04/15 09:24 PM (8 years, 6 months ago)

:rofl::rofl::rofl:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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InvisibleToadstool5
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Mad Season]
    #21898604 - 07/04/15 09:26 PM (8 years, 6 months ago)

Fuck! Gravity whooped her ass! :rofl:

Her boobs are morphing into an extra pair of arms :eek:


--------------------
If you do not know where the mushroom products you are consuming are grown, think twice before eating them. :badshroom:
- Paul Stamets

AMU Teks :mushroom2: Stro's Write Ups


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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Inextinctus]
    #21898613 - 07/04/15 09:27 PM (8 years, 6 months ago)

Quote:

Inextinctus said:
Curious to know, with the whole outside/inside air transfers... Wouldn't it make more sense to actually attach your arm covers, whatever they may be, to your box so that there's an air tight seal and you can slide your hands out with ease? No sleeves attached?
Keep flammable substances out of the box and boom? (No pun intended)



no it wouldnt' make any sense, just follow the teks as stated becasue there stated for good reason1 no flaming inside don't attach your gloves, wet your sab and get to work


--------------------

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OfflineInextinctus
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Buck513]
    #21898615 - 07/04/15 09:27 PM (8 years, 6 months ago)

Quote:

Buck513 said:
.



^^^
Up your post count like a pro


--------------------
Soundcloud.com/inextinctus
what are we but a different combination of the elements we consume?
"So then I :lsd:"



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InvisibleInocuole
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Inextinctus]
    #21898621 - 07/04/15 09:29 PM (8 years, 6 months ago)

Quote:

Inextinctus said:
Quote:

Buck513 said:
.



^^^
Up your post count like a pro




8 posts in and you're already being a shit talker?


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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InvisibleBuck513

Registered: 04/17/14
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Inextinctus]
    #21898623 - 07/04/15 09:29 PM (8 years, 6 months ago)

Quote:

Inextinctus said:
Quote:

Buck513 said:
.



^^^
Up your post count like a pro



Actually I deleted the granny tits cause I didn't feel like getting banned again.

Get with the program


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


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OfflineInextinctus
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: cronicr]
    #21898625 - 07/04/15 09:30 PM (8 years, 6 months ago)

Cool, just checking. In my head it seemed like a solid idea but for some reason I feel like I should trust you :rasta:


--------------------
Soundcloud.com/inextinctus
what are we but a different combination of the elements we consume?
"So then I :lsd:"



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InvisibleInocuole
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Inextinctus]
    #21898629 - 07/04/15 09:30 PM (8 years, 6 months ago)

Quote:

Inextinctus said:
Cool, just checking. In my head it seemed like a solid idea but for some reason I feel like I should trust you :rasta:





  That might be why.  And .


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
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OfflineInextinctus
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Inocuole]
    #21898635 - 07/04/15 09:31 PM (8 years, 6 months ago)

Haha no sir, I respect everyone here! I thought it was funny actually.
I'm on my phone right now though so thread updating is like 2 old people fucking ;D


Edited by Inextinctus (07/04/15 09:35 PM)


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InvisibleBuck513

Registered: 04/17/14
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: cronicr]
    #21898643 - 07/04/15 09:33 PM (8 years, 6 months ago)

Quote:

cronicr said:
Quote:

Inextinctus said:
Curious to know, with the whole outside/inside air transfers... Wouldn't it make more sense to actually attach your arm covers, whatever they may be, to your box so that there's an air tight seal and you can slide your hands out with ease? No sleeves attached?
Keep flammable substances out of the box and boom? (No pun intended)



no it wouldnt' make any sense, just follow the teks as stated becasue there stated for good reason1 no flaming inside don't attach your gloves, wet your sab and get to work



:bye: cron
Have fun fishing


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OfflineInextinctus
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Inocuole]
    #21898650 - 07/04/15 09:35 PM (8 years, 6 months ago)

Quote:

Inocuole said:
Quote:

Inextinctus said:
Cool, just checking. In my head it seemed like a solid idea but for some reason I feel like I should trust you :rasta:




  That might be why.  And .



No.. No I think it's more intimate than that... :crazy2:


--------------------
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what are we but a different combination of the elements we consume?
"So then I :lsd:"



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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Buck513] * 1
    #21898657 - 07/04/15 09:36 PM (8 years, 6 months ago)

will do brutha! see you all soon!...fucking rides taking forever lol


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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Inextinctus]
    #21898668 - 07/04/15 09:38 PM (8 years, 6 months ago)

Quote:

Inextinctus said:
Quote:

Inocuole said:
Quote:

Inextinctus said:
Cool, just checking. In my head it seemed like a solid idea but for some reason I feel like I should trust you :rasta:




  That might be why.  And .



No.. No I think it's more intimate than that... :crazy2:




cron is a very handsome man, I can relate, buddy.  :derfase:


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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Inocuole]
    #21898684 - 07/04/15 09:41 PM (8 years, 6 months ago)

:pantytail:


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It doesn't matter what i think of you...all that matters is clean spawn

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OfflineMachiavelliavore
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Inextinctus]
    #21898850 - 07/04/15 10:20 PM (8 years, 6 months ago)

I'd like to hear an argument against what I'm saying:

Flaming in the box creates more turbulence but less air exchange with the outside.  The flame creates a pressure spike in the box that will force a small amount of air out, then suck it back in as the temperature drops again.  I highly doubt the amount of air exchanged in this process is anywhere near the air displacement of the volume of two human arms.  For each 1 deg C the lighter changes the air temperature, an expected displacement of ~1/300ths of the air volume in the box should occur, or .2qt per deg C in my airbox.  I would guess each human arm is around a quart, so the air temperature in the box would have to change by around 10 Celsius to create a similar displacement.  Based on PV=nRT.

Since the inside of the box is covered in bleach water/soapy water, there are few locations where mold spores can be resting, except already floating in the air.  Outside air that hasn't be sprayed with bleach water probably has significantly higher spore concentration.

If people want to oust/lysol/bleach an entire room and can really shut down any air movement in their house, that probably does make it absolutely better to flame outside.  However if there is air movement and you don't wanna get bleach all over your wood floors, cabinets, and food, I think there's an argument for limiting contact with the outside.


Quote:

Inextinctus said:
Curious to know, with the whole outside/inside air transfers... Wouldn't it make more sense to actually attach your arm covers, whatever they may be, to your box so that there's an air tight seal and you can slide your hands out with ease? No sleeves attached?
Keep flammable substances out of the box and boom? (No pun intended)




They're just plastic vegetable bags I slice the end off off and taped them around the holes.  They're a little bit floppy, but once my arms are in, there is very little airspace, and it would take an air current paralel in direction to my arms to cause a significant exchange with the air in the box IMO.  Because I don't work in an especially clean area, I think it's a solid idea and it works for me.  I just keep a lid on a small container of alcohol inside so the fumes don't build up inside the box.  I also wear tyvek sleeves to avoid bleach burns or skin particles falling in the box as easily.



re: my credibility, I don't have a lot, I just experiment and quickly discard tactics that don't work.  Just my opinion, but opinion not science is mostly what you will get on this specific topic.  I'd be curious if greenrabbit has done anything on this, his monotub analysis was impressive.

I've used v-tek, cased straight grains, pf tek for kicks, MSG 3 quart foil pans with CVG, tried grainwater coir.  I've made a lot of mistakes from experimenting and doing shit like g2ging an entire container, then pouring fresh grain back in to see if I could get more inoculant out of the scraps, then not noticing that contaminated a bit and screwing over many jars.  I've done plenty of stupid shit.  I like to see what I can get away with.  A lot of it failed, but I don't advise people to do it.  Flaming in my 60qt airbox has not caused my any problems that I'm aware of.  My syringes, poptop syringe inoculations, and cloning have all been relatively contam free.  I did get the best iso I've seen yet from doing something dumb though :smile:


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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InvisibleMad Season
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Machiavelliavore]
    #21898890 - 07/04/15 10:28 PM (8 years, 6 months ago)

Quote:

Machiavelliavore said: Since the inside of the box is covered in bleach water/soapy water, there are few locations where mold spores can be resting, except already floating in the air.  Outside air that hasn't be sprayed with bleach water probably has significantly higher spore concentration



:facepalm: why do you think this? It's called a still air box. Not a sterile box for a reason. The whole point is to have still air. The less air moving the better. That includes arms. G2ging with qt jars can even cause too much movement, and has ruined lots of peoples g2g. You want to have as little of a surface area moving around. Thus using bulky gloves or liners is just moving air around more. Wipe a slide with a sanitizer (note not considered a sterilizer) and see how many spores are left. It just looks like you moved them around.


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Edited by Mad Season (07/04/15 10:30 PM)


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OfflineMachiavelliavore
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Mad Season]
    #21899043 - 07/04/15 11:02 PM (8 years, 6 months ago)

It's a statistics game.  If the air inside the box has significantly reduced spore count from having bleach mist blasted through it,and the surfaces have been wiped down and subsequently sprayed, it stands to reason that most spores that come to rest on said bleachwater will not be simply agitated into the air again.  It could be plain water.  The concept is to get them out of the air, then keep'm down regardless of whether you kill them, yes?

It then stands to reason that the spore concentration present in the air in the box should be lower than it is outside where fine mist has not been passed through the air.

I also agree, less air movement is better.  The argument is whether it is better to exhange air with the outside or agetate the air inside, which would depend heavily on how much worse the outside air was.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Machiavelliavore]
    #21899053 - 07/04/15 11:07 PM (8 years, 6 months ago)

Quote:

people want to oust/lysol/bleach an entire room and can really shut down any air movement in their house, that probably does make it absolutely better to flame outside.




Hells yeah! my place looks like an operating room when im done with it and it...

:feelsgoodman:

I could probably do without a SAB but what self-respect would i have if i didnt use one :rofl:


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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Toadstool5]
    #21899063 - 07/04/15 11:10 PM (8 years, 6 months ago)

I'm quite opposite lol. Don't even have sleeves or use sanitizer. Do it in a kitchen or living room. Just in a big ass 110 qt tub. Go soap and water! :smile:

As for the outside air. It is definitely dirty and it will still come in. It should however be still enough inside to fall to the ground. It takes a particle .5 microns at 5 feet high 45 minutes-an hour to fall to the ground. It's a lot better not to stir any air in there. Because particles will be all over and take awhile to settle again. Every millimeter squared has over 200 thousand spores on it. This is also why you NEVER have anything but your scalpel or needle go over open media. It's much better for air to be completely still for the best results. And still for a long ass time.


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Edited by Mad Season (07/04/15 11:19 PM)


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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Mad Season]
    #21899118 - 07/04/15 11:34 PM (8 years, 6 months ago)

Thats something i always watch too.

I work as high up as i can and thats with non-porous flooring and counters. The floor is essentially disgusting when you think about it, fuck the three second rule :scat:


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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Mad Season]
    #21899154 - 07/04/15 11:43 PM (8 years, 6 months ago)

I'm working in a room with a fridge, an agacent drafty room, unrestricted air access to sub level, hood, adjacent rooms, lots of dust, food etc, so obtaining still air conditions there is probably not that likely, which is why I use a sort of ghetto glovebox setup.

If I had an empty, unused room where the air flow could really stop and was possible to keep cleaner, then I'd probably be less concerned about outside air getting in.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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