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OfflinePutACapInHisAss
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Is There a Flame Sterilization Tek for Still Air Boxes?
    #21897620 - 07/04/15 05:11 PM (8 years, 6 months ago)

I know this seems super simple, but when using a still air box, there must be a proper way. I see that most people do it outside of the box to avoid creating air currents or lighting up alcohol based cleaners used inside.

If you are inoculating multiple containers, do you flame outside of the box between each one, every other one, once per session...? What else to know? Is it possible to damage the needle or the contents? Is a lighter good enough or must I have an alcohol lamp? Wouldn't bringing it in and out of a still air box many times create vacuums moving around air in and out of the box, and possibly getting spores on the surface of the needle not flamed?


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OfflineSyntheticwords
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: PutACapInHisAss]
    #21897811 - 07/04/15 05:44 PM (8 years, 6 months ago)

I always flame my needle, scalpel, loop whatever I use outside the box

I used to use a lighter in the box and never had much worries aside from my thumb catching on fire from using ISO alchol on the sterilization port
Just make sure you don't spray a whole bunch of Lysol and light up the flame though...


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InvisibleToadstool5
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: PutACapInHisAss]
    #21897864 - 07/04/15 05:56 PM (8 years, 6 months ago)

A lighter works but is not as hot so it takes longer, they are also a pain to use with gloves on. I personally use a propane torch for gourmet when i do pf-tek (no difference)

Flame between every jar but not between holes.

When you take it out, move very slowly and always wear a surgical mask (bouffant caps help too) then move the flamed needle back into the SAB while it is still red hot so anything that lands on it will die.

When flaming be careful to not melt the luer lock on the syringe and be careful with red hot needles. When they are hot they become really soft and are easy to bend by mistake.

Never light up inside the SAB, its not necessary or safe.

If you are really worried about air currents: clean the entire room with 3% bleach (allow it to sit for 10min), allow the room to settle by turning off the ac and fans for one hour, run a clean HEPA filter for awhile, turn it off and spray lysol everywhere right before you work. Remember to wear a surgical mask and use a shower cap or bouffant cap for dander and tyvek sleeves to help prevent dander and air currents from making their way through the SAB holes.

You will figure it out! We all stress how important sterility is but that doesnt mean its difficult, you only need vigilance and a deep cleaning :thumbup:


--------------------
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InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Toadstool5]
    #21898184 - 07/04/15 07:28 PM (8 years, 6 months ago)

A lighter can be used, but its inferior to butane torches and stuff like that. But if its all you got, its all you got:shrug:

Never sterilize inside SAB, also remember its good to let the air settle every time you bring your hands back in the SAB for a good 20-30 seconds. That time is also used so the tools can have a chance to cool down a bit.


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OfflineMachiavelliavore
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: PutACapInHisAss]
    #21898246 - 07/04/15 07:48 PM (8 years, 6 months ago)

I've tried a few techniques.  In the past I just said fuck it and used the lighter in the box, because I'd rather stir up what inside it than bring in air current from outside that haven't been bleach nuked by taking my arms in and out over and over.

If you're doing lid pops, I now prepare all the lids (get them very lose/remove rings,) then flame my needle outside, and go from jar to jar, squirt squirt.  If I have to re-arrange shit in the airbox to do another set, I'll reflame.
IMO:
a) Any contams that land on the tiny surface area of the syringe needle will ABSOLUTELY fall out of the air into the containers when the lids are opened, and in far greater concentration.  On top of that, only the very tip of the needle would transfer these contams into the jars.

b) Any contams in the syringe are unaffected by flaming.

c) If you're using ports/poly, flame every time, since you are rubbing the needle against shit and it's literally the only thing entering the jar.  Flame in the air box (after any more sensitive work is completed) if you bother to use one.

Just try to be as fast and smooth as possible if opening lids, and try to keep your hands from being above the open container.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Machiavelliavore]
    #21898262 - 07/04/15 07:52 PM (8 years, 6 months ago)

Please don't advise someone to flame sterilize inside a SAB.
Quote:

Machiavelliavore said:
In the past I just said fuck it



That type of attitude will get you literally nowhere in this hobby.


--------------------
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OfflineMachiavelliavore
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Buck513]
    #21898312 - 07/04/15 08:06 PM (8 years, 6 months ago)

Do you have some scientific studies that say using a lighter in the a confined space the size of a glovebox creates more turbulance than moving your arms in and out?

I have sleeves on my air box, so my turbulance is increased going in and out, and the interaction with outside air due to internal turbulance is decreased, so I would rather flame inside my air box than go in and out constantly.  If I only need to flame something once (say a needle tip) now I will do it outside rather than flaming it between every jar (which as I outlined, I believe to be illogical when popping lids.)

Certainly the air spore concentration inside the glove box is considerably less inside the air box than outside, therefore I would rather create some turbulance in the internal air than exchange a significant amount of air with the outside.

I said "fuck it" because both options are rather shitty in my opinion.  I've had very few trich/mold problems.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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InvisibleBuck513

Registered: 04/17/14
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Machiavelliavore]
    #21898323 - 07/04/15 08:09 PM (8 years, 6 months ago)

Quote:

Machiavelliavore said:
Do you have some scientific studies that say using a lighter in the a confined space the size of a glovebox creates more turbulance than moving your arms in and out?

I have sleeves on my air box, so my turbulance is increased going in and out, and the interaction with outside air due to internal turbulance is decreased, so I would rather flame inside my air box than go in and out constantly.  If I only need to flame something once (say a needle tip) now I will do it outside rather than flaming it between every jar (which as I outlined, I believe to be illogical when popping lids.)

Certainly the air spore concentration inside the glove box is considerably less inside the air box than outside, therefore I would rather create some turbulance in the internal air than exchange a significant amount of air with the outside.

I said "fuck it" because both options are rather shitty in my opinion.  I've had very few trich/mold problems.



:doublefacepalm:


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InvisibleInocuole
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Buck513]
    #21898344 - 07/04/15 08:15 PM (8 years, 6 months ago)

Not only does that create air currents, but some people spray the inside of the SABs with isopropyl and still more people aren't the smartest in their pay grade, so naturally they will blow all the hair off their arms and maybe their face taking that advice.  I flame outside the SAB and it's fantastic.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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InvisibleMad Season
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Inocuole]
    #21898377 - 07/04/15 08:24 PM (8 years, 6 months ago)

:whathesaid: even a qt sized grain jar can cause too much air movement during g2gs. That's why you need a big box to reduce the amount of air movement.

You need clean air to burn a flame. Heat rises too. It's no different than having a fire in your living room. Principles still apply.


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Edited by Mad Season (07/04/15 08:26 PM)


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InvisibleInocuole
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Mad Season]
    #21898391 - 07/04/15 08:28 PM (8 years, 6 months ago)

Quote:

Mad Season said:
:whathesaid: even a qt sized grain jar can cause too much air movement during g2gs. That's why you need a big box to reduce the amount of relative air movement




Edited for science.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
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InvisibleMad Season
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Inocuole]
    #21898394 - 07/04/15 08:29 PM (8 years, 6 months ago)

LOL. :ohyou:


--------------------
contam and car window art
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InvisibleBuck513

Registered: 04/17/14
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Mad Season]
    #21898447 - 07/04/15 08:45 PM (8 years, 6 months ago)

Science:ancientaliens:


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OfflineMachiavelliavore
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Inocuole]
    #21898504 - 07/04/15 09:01 PM (8 years, 6 months ago)

Obviously you shouldn't light up in an air box covered with flammables.


If many repetitive flamings are required, it's nice not to have to clean gloves and sleeves over and over due to outside air exposure.  I'm not saying it's always right to flame inside, I'm saying there are trade offs to either option, especially if you have sleeves which make it harder to come in and out of your airbox, or you forgot about that moldy tomato 10 feet away :smile:  Sometimes I'd rather create turbulence in a cleaner environment than venture into a dirty one to spare it.

Again, I use plastic bags around my arms that extend off the holes, so I'm a bit more isolated from the outside that a standard 2 holes n nothin' type box.

I've had plenty of success with direct clone tissue samples onto grain petris while flaming an alcohol covered knife in my box.  It has been my experience that most contams come from bad inoculation sources or endospores.

Glad to see how you got to 3.5k posts in a year buck  :projectile:


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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InvisibleMad Season
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Machiavelliavore]
    #21898518 - 07/04/15 09:05 PM (8 years, 6 months ago)

You probably actually wouldn't want to see all his posts LOLOL.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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InvisibleBuck513

Registered: 04/17/14
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Machiavelliavore]
    #21898530 - 07/04/15 09:08 PM (8 years, 6 months ago)

How many tubs have you grown?
What teks do you follow? Or are you such a myco guru you don't follow teks?

Go look at my posts if you want fool. I don't know what you mean by "I see how you got 3.5k posts".


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InvisibleBuck513

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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Mad Season]
    #21898540 - 07/04/15 09:11 PM (8 years, 6 months ago)

Quote:

Mad Season said:
You probably actually wouldn't want to see all his posts LOLOL.



Probably not. I tend to be rather blunt and I come off as condescending sometimes. But I'm really not. I like laughing at people though:shrug:
And people laugh at me. Who gives a shit. Its a drug forum. I'm glad to offer up the small bit of knowledge I have, I'm even more glad o learn something I didn't already know.
Too many over sensitive people are around these days.


--------------------
Fail to plan and you plan to fail.

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OfflineInextinctus
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Machiavelliavore]
    #21898561 - 07/04/15 09:15 PM (8 years, 6 months ago)

Curious to know, with the whole outside/inside air transfers... Wouldn't it make more sense to actually attach your arm covers, whatever they may be, to your box so that there's an air tight seal and you can slide your hands out with ease? No sleeves attached?
Keep flammable substances out of the box and boom? (No pun intended)


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InvisibleMad Season
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Buck513]
    #21898567 - 07/04/15 09:17 PM (8 years, 6 months ago)

Word buck. Definitely best place to work on this hobby :smile:. Just thinking about the granny tits quoted from earlier. :sick: haha


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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InvisibleBuck513

Registered: 04/17/14
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Re: Is There a Flame Sterilization Tek for Still Air Boxes? [Re: Mad Season]
    #21898583 - 07/04/15 09:22 PM (8 years, 6 months ago)

.


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Edited by Buck513 (07/04/15 09:25 PM)


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