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Invisibleuncle_rico
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Re: Post Your Cultivation Picture of The Day [Re: Aero]
    #21897410 - 07/04/15 04:29 PM (8 years, 10 months ago)

Life Cycle in a Petri Dish

or, what happens when you forget about an aggressive culture ....

kernel of grain on agar and forgotten


more time passes





combination clone and spore print!


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OfflineBlazinAmazin
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Re: Post Your Cultivation Picture of The Day [Re: uncle_rico]
    #21897561 - 07/04/15 04:59 PM (8 years, 10 months ago)

That is awesome!


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InvisibleMudaFuka
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Re: Post Your Cultivation Picture of The Day [Re: uncle_rico]
    #21897834 - 07/04/15 05:48 PM (8 years, 10 months ago)

Quote:

uncle_rico said:
Life Cycle in a Petri Dish

or, what happens when you forget about an aggressive culture ....

kernel of grain on agar and forgotten


more time passes





combination clone and spore print!



I've been starting all my grows with petri clones for a while. It's a great way to get clean spores too.


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Offlinemushpunx
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Re: Post Your Cultivation Picture of The Day [Re: MudaFuka]
    #21898709 - 07/04/15 09:46 PM (8 years, 10 months ago)

Quote:

MudaFuka said:
Quote:

uncle_rico said:
Life Cycle in a Petri Dish

or, what happens when you forget about an aggressive culture ....

kernel of grain on agar and forgotten


more time passes





combination clone and spore print!



I've been starting all my grows with petri clones for a while. It's a great way to get clean spores too.






Why are you putting whole fruits on the plate? I understand just dropping a tiny pin, but a whole fruit? I dont want spores tainting my clone !


Just curious!


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Edited by mushpunx (07/04/15 09:56 PM)

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OfflineShroominMe
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Re: Post Your Cultivation Picture of The Day [Re: mushpunx]
    #21898742 - 07/04/15 09:53 PM (8 years, 10 months ago)

Quote:

mushpunx said:

Why are you putting whole fruits on the plate? I understand just dropping a tiny pin, but a whole fruit? I dont want spores tainting my clone !

Not to mention whatever is lurking on the fruit body. I tear the fruit and biopsy a tiny piece of virgin tissue to drop

Just curious!



Did you read his post?  He said:

Quote:

kernel of grain on agar



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Invisiblegrownright
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Re: Post Your Cultivation Picture of The Day [Re: mushpunx]
    #21898744 - 07/04/15 09:53 PM (8 years, 10 months ago)

Quote:

mushpunx said:
Why are you putting whole fruits on the plate? I understand just dropping a tiny pin, but a whole fruit? I dont want spores tainting my clone !

Not to mention whatever is lurking on the fruit body. I tear the fruit and biopsy a tiny piece of virgin tissue to drop

Just curious!



no, hes saying you leave an isolate in a dish and leave the mycelium to fully colonize until it pins in the dish, then you clone the pin. It allows for a much cleaner specimen to clone from and you know youll be isolating an aggressive, fruiting strain.

I actually have an isolate right now that I started from an invitro pin that had grown on my first brf paste jar. I wanted to practice before trying agar, but had forgotten about the jar. I found a pin in it one day and I had poured some agar that morning so I cloned it. I cant wait to see how it turns out :smile:


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Edited by grownright (07/04/15 09:56 PM)

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Offlinemushpunx
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Re: Post Your Cultivation Picture of The Day [Re: blindingleaf]
    #21898753 - 07/04/15 09:54 PM (8 years, 10 months ago)

Quote:

blindingleaf said:
:jawdrop:

do u do golf courses too?





:rofl:


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Offlinemushpunx
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Re: Post Your Cultivation Picture of The Day [Re: grownright]
    #21898771 - 07/04/15 09:58 PM (8 years, 10 months ago)

Quote:

grownright said:
Quote:

mushpunx said:
Why are you putting whole fruits on the plate? I understand just dropping a tiny pin, but a whole fruit? I dont want spores tainting my clone !

Not to mention whatever is lurking on the fruit body. I tear the fruit and biopsy a tiny piece of virgin tissue to drop

Just curious!



no, hes saying you leave an isolate in a dish and leave the mycelium to fully colonize until it pins in the dish, then you clone the pin. It allows for a much cleaner specimen to clone from and you know youll be isolating a fruiting strain.





:facepalm:


Haha no I didnt see it was Muda's photo and I didnt really read it Im a little loopy hah

I just looked at the picture and was like "did he just drop a whole fruit on a plate and let it grow" ?
I was like how are you gunna get clean growth off of that

Didnt realize those were pitres left to fruit :facepalm: thats embrrasing


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Edited by mushpunx (07/04/15 10:02 PM)

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InvisibleMudaFuka
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Re: Post Your Cultivation Picture of The Day [Re: mushpunx]
    #21898774 - 07/04/15 09:59 PM (8 years, 10 months ago)

Those fruits grew on the plates those are in-vitro pins that I left to mature and drop spores.
I always weight for a pin to form on my agar before starting a grow with a new variety. It lets me get a clone without going threw a whole grow and in-vitro petri pins are almost always really strong fruiters and fast pinners.

hear are a few more

enoki

lions mane


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Offlinemushpunx
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Re: Post Your Cultivation Picture of The Day [Re: MudaFuka]
    #21898791 - 07/04/15 10:05 PM (8 years, 10 months ago)

Quote:

MudaFuka said:
Those fruits grew on the plates those are in-vitro pins that I left to mature and drop spores.
I always weight for a pin to form on my agar before starting a grow with a new variety. It lets me get a clone without going threw a whole grow and in-vitro petri pins are almost always really strong fruiters and fast pinners.

hear are a few more

enoki

lions mane





Yea dude I gotcha. I grab clones like this too sometimes

I looked at the photo and thought it was a whole fruit dropped onto a plate :lol: it 4th of july and Im feeling loopy


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Invisibleeatyualive
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Re: Post Your Cultivation Picture of The Day [Re: mushpunx]
    #21898813 - 07/04/15 10:09 PM (8 years, 10 months ago)

liquid culture from 2007.



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InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: Post Your Cultivation Picture of The Day [Re: eatyualive]
    #21898824 - 07/04/15 10:13 PM (8 years, 10 months ago)

How often will a plate produce a pin? Should you add more agar since it will be sitting around for a long time?


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InvisibleMad Season
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Re: Post Your Cultivation Picture of The Day [Re: Buck513]
    #21898836 - 07/04/15 10:15 PM (8 years, 10 months ago)

:O eat! That looks delicious!


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InvisibleMudaFuka
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Re: Post Your Cultivation Picture of The Day [Re: Buck513]
    #21898864 - 07/04/15 10:22 PM (8 years, 10 months ago)

It depends on genetics. I've had some dishes pin just a couple days after the mycelium hits the side of the dish. Others like PE take several weeks. I don't add any extra agar. It doesn't dry out as fast as you might think. I've got plates sitting at room temperature that are almost a year old and they still aren't completely dried out. Some of them have put out multiple flushes. Lol


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Invisibleeatyualive
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Re: Post Your Cultivation Picture of The Day [Re: MudaFuka]
    #21898912 - 07/04/15 10:33 PM (8 years, 10 months ago)

i had a tex yellow cap pin before the plate was fully colonized. the ape pins fast as well on a plate. but slow as shit on subs for me.


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InvisibleMudaFuka
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Re: Post Your Cultivation Picture of The Day [Re: eatyualive]
    #21898942 - 07/04/15 10:40 PM (8 years, 10 months ago)

Jon Allens pin the fastest for me on agar. KSSS and PE6 are close seconds.


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Re: Post Your Cultivation Picture of The Day [Re: MudaFuka]
    #21899005 - 07/04/15 10:53 PM (8 years, 10 months ago)

Quote:

MudaFuka said:
It depends on genetics. I've had some dishes pin just a couple days after the mycelium hits the side of the dish. Others like PE take several weeks. I don't add any extra agar. It doesn't dry out as fast as you might think. I've got plates sitting at room temperature that are almost a year old and they still aren't completely dried out. Some of them have put out multiple flushes. Lol





You leave them wrapped tho yea?



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Edited by mushpunx (07/04/15 11:05 PM)

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InvisibleMudaFuka
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Re: Post Your Cultivation Picture of The Day [Re: mushpunx]
    #21899037 - 07/04/15 10:59 PM (8 years, 10 months ago)

yeah.


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Re: Post Your Cultivation Picture of The Day [Re: MudaFuka]
    #21899059 - 07/04/15 11:08 PM (8 years, 10 months ago)

Ive never had much sucess getting plates to pin. When they have in the past Ive cloned them with good results.

Ill let plates grow out amd leave them arpund for ages w out pin

Any tips/tricks dude?


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InvisiblePastywhyteMDiscord
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Re: Post Your Cultivation Picture of The Day [Re: mushpunx]
    #21899108 - 07/04/15 11:31 PM (8 years, 10 months ago)

Extra nutes can help a bit with pinning. More starch less sugar also helps. MEA pins poor, grain water, potato starch and dog food seem to do better.

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