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Invisibleoontribe
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Registered: 01/14/15
Posts: 3,570
cloning question
    #21896082 - 07/04/15 09:53 AM (8 years, 6 months ago)

How long would a tissue take to colonize the jar?


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
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Re: cloning question [Re: oontribe] * 1
    #21896251 - 07/04/15 10:57 AM (8 years, 6 months ago)

Depends on how large of a tissue sample and how well you prepped the grain jar. But I would definitely clone the mushroom to an agar plate first to be sure there aren't any contaminates, wait for it to colonize, then transfer a healthy piece to the grain jar. :thumbup:


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Invisibleoontribe
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Re: cloning question [Re: Psilosoulful]
    #21896396 - 07/04/15 11:44 AM (8 years, 6 months ago)

Could it happen that sometimes the tissue wouldnt colonize?


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OfflinePsilosoulful

Registered: 09/05/14
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Last seen: 1 year, 1 month
Re: cloning question [Re: oontribe]
    #21896541 - 07/04/15 12:28 PM (8 years, 6 months ago)

Quote:

oontribe said:
Could it happen that sometimes the tissue wouldnt colonize?



If you take the tissue from inside the stem of a freshly picked mushroom, I don't see why it wouldn't colonize. The mycelium is still active and putting it on a nutritious medium, such as an agar plate, would trigger more growth. Try it out and post your results!! :dancer:


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Invisibleoontribe
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Re: cloning question [Re: Psilosoulful]
    #21896556 - 07/04/15 12:31 PM (8 years, 6 months ago)

Lol thanks dude :smile:


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InvisibleSteveRogers
gandy dancer
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Registered: 10/24/06
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Re: cloning question [Re: oontribe]
    #21896773 - 07/04/15 01:36 PM (8 years, 6 months ago)

Quote:

oontribe said:
Could it happen that sometimes the tissue wouldnt colonize?




On the rare occasion I have a set of jars not colonize it's because there was a hidden contaminant on the plate I took wedges from. What is your cloning process/sterile technique like?


--------------------
"General, I am loyal to nothing......except The Dream"


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Invisibleoontribe
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Registered: 01/14/15
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Re: cloning question [Re: SteveRogers]
    #21897027 - 07/04/15 02:49 PM (8 years, 6 months ago)

Well iam cloning to a sterilized half pint jar of grains (cant work with agar now) i sterilized the knife in a pc wearing gloves sanitised with alchol and transfered the tissue inside a sab.


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Invisiblefilthyknees
no coincidence
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Registered: 03/08/13
Posts: 6,283
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Re: cloning question [Re: oontribe]
    #21897048 - 07/04/15 02:53 PM (8 years, 6 months ago)

that will likely fail, I would do at least ten attempts now and hope for maybe one success

http://www.shroomery.org/forums/showflat.php/Number/17784996#17784996


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


Edited by filthyknees (07/04/15 02:59 PM)


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