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OfflinefirstTIMER420
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AGAR questions
    #21896047 - 07/04/15 09:32 AM (8 years, 6 months ago)

hey all, i am looking to get into agar, because im starting a couple monotubs and using grains(duh) and would like a clean inoculant, instead of a MS syringe. 

Now, there are many different ways to make agar paste as ive read on here, but its tricky to get the mixture just right.  Basically there are a couple different mixtures that are common(majority of people use) PDA being one of them.

I was wondering if it would be better to buy the ingrediants separately and make my own agar mixture, or to just buy the pre mixed agar paste-powder(PDA mixture for instance) and just use that.  What is your opinions on that?  I know it would be better to learn how to mix them together, and probably cheaper.  at a quick glance, it was 16.50 for an 8-oz PDA bottle.  Now, it says that could make up to 100 petris, but im wondering how many that could REALISTICLY make.

Also, what agar recipie would be good to start with, as well as relatively cheap?  Please include brands of products(agar powder, dextrose and such)

The last thing, i saw that they have tri separated petri dishes, with 3 sections for growing cultures.  Would you recommend to use these instead of the regular petris? If so, why?


Thanks for the input guys its very much appreciated!


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InvisiblebodhisattaMDiscordReddit
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Re: AGAR questions [Re: firstTIMER420]
    #21896066 - 07/04/15 09:39 AM (8 years, 6 months ago)

Buy agar flakes at grocery store.Asian store. Telephone brand is easy to find or order online.


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InvisibleGrey
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Re: AGAR questions [Re: bodhisatta]
    #21896141 - 07/04/15 10:16 AM (8 years, 6 months ago)

Agar is easy to make. For PDA you just need some instant potato flakes, honey or karo, and agar agar powder.



Check out Pastywhyte's Easy Agar Tek

There's lots of ways to do agar. I only order non-sectioned petris for pouring.

MEA is easy to prep as well using this (pic below) and agar.



Here's a bunch of cool videos w/ agar prep.


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InvisiblebodhisattaMDiscordReddit
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Re: AGAR questions [Re: Grey]
    #21896180 - 07/04/15 10:30 AM (8 years, 6 months ago)

:whathesaid: :rockon:
Check out the 3 and 4th link in my signature too. And look up stro's cleaning and isolating on agar


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OfflineOPB
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Re: AGAR questions [Re: bodhisatta]
    #21896213 - 07/04/15 10:44 AM (8 years, 6 months ago)

I opted for the premixed powder. The agar agar I found locally was very expensive and looking online the prices for each ingredient add up close to the cost of the mix. I'm sure in large quantities it's more efficient to mix it yourself though.


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InvisibleMad Season
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Re: AGAR questions [Re: bodhisatta]
    #21896221 - 07/04/15 10:45 AM (8 years, 6 months ago)

I made this 2 days ago on sterile techniques. Still needs some work, but it's nonetheless a damn good place to start.

http://www.shroomery.org/forums/showflat.php/Number/21889950

All the other stuff suggested too. Idk how you wouldn't be good at agar after all this lol


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How to shroomery like a pro! (Seriously, everyone read this!)
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OfflinefirstTIMER420
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Re: AGAR questions [Re: Grey]
    #21896860 - 07/04/15 02:03 PM (8 years, 6 months ago)

I think im going to go with MEA, and thanks for all the vids and responses, helped me bunches and made me decide i am going to make the jump to agar.  Although i haven't had any contams yet, i want to keep it that way.

The only thing i need to work on is being able to identify bacteria in the culture as right now i have no idea how yall do it, unless its REALLY apparent


EDIT: can you use dark MALT extract as well?


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Edited by firstTIMER420 (07/04/15 02:06 PM)


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OfflinefirstTIMER420
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Re: AGAR questions [Re: firstTIMER420]
    #21896882 - 07/04/15 02:10 PM (8 years, 6 months ago)

and also, as far as MEA, will 1LB be fine for say 20 plates?


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InvisibleMad Season
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Re: AGAR questions [Re: firstTIMER420]
    #21896889 - 07/04/15 02:12 PM (8 years, 6 months ago)

Dark malt will work. Also in the thread I sent, really close to the beginning, you'll see a section called what to look out for. It has a link to this:

http://www.shroomery.org/forums/showflat.php/Number/21865514

For 20 plates, more like 20 grams.


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contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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OfflinefirstTIMER420
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Re: AGAR questions [Re: Mad Season]
    #21896899 - 07/04/15 02:14 PM (8 years, 6 months ago)

i was going to purchase the 3lb bag, but ill go with the 1LB.


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InvisibleGrey
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Re: AGAR questions [Re: firstTIMER420]
    #21896907 - 07/04/15 02:17 PM (8 years, 6 months ago)

The MEA will stretch, you don't need much. Just keep the extract powder dry. It can clump up in humid air.


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InvisibleJuiceh
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Re: AGAR questions [Re: firstTIMER420]
    #21896937 - 07/04/15 02:26 PM (8 years, 6 months ago)

That's a good pick. I started out with MEA because that gave me the option to also use the ME to make LC. I've also made PDA with potato broth + dextrose. As well as a few experiments with water used to boil wood chips and shit like that. MEA is very reliable. It's good to switch up the mix every once in a while.


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Re: AGAR questions [Re: Juiceh]
    #21897105 - 07/04/15 03:07 PM (8 years, 6 months ago)

anything used in agar can be used in a lc recipe too and vice versa.

the best lc recipes has 2% agar in them! :eek:


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InvisibleJuiceh
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Re: AGAR questions [Re: spacechildo]
    #21897556 - 07/04/15 04:59 PM (8 years, 6 months ago)

Quote:

spacechildo said:
anything used in agar can be used in a lc recipe too and vice versa.




:mindblown:

I been doing alot of GWLC lately. Kinda pointless to do anything else when so much grain water is available after making a few batches of grain. Making fucktons of Morel LC to dump around my property and families properties. Got a few gallons of LC going right now! :grin:


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How I make 15 lbs of casing in a 941. How I line 12 shoeboxes with 1 39gal bag. How I Deep Fry Cubes!
RogerRabbit said:You need a bigger pressure cooker for this hobby. RR


Edited by Juiceh (07/04/15 05:01 PM)


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OfflinefirstTIMER420
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Re: AGAR questions [Re: Juiceh]
    #21903099 - 07/05/15 10:06 PM (8 years, 6 months ago)

might as well start making LC then lol,

grain water is whats left over after boiling the grains right.


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InvisibleMad Season
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Re: AGAR questions [Re: firstTIMER420]
    #21903153 - 07/05/15 10:22 PM (8 years, 6 months ago)

What are you going to make lcs with? Think of lc as a g2g replacement and agar as an inoculation replacement. Every project has to start with agar so you should inoculate an lc with a clean agar wedge.


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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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OfflinefirstTIMER420
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Re: AGAR questions [Re: Mad Season]
    #21903191 - 07/05/15 10:36 PM (8 years, 6 months ago)

idk tbh i haven't even looked up LC's at all.  Agar is an universal inoculant and i guess if you used it to innoc LC like you said, all it would take for grains is a couple of drops in each jar of grain to innoc it.  Hmmm, i gotta look more into it, but my problem with this stuff and LC's is knowing how to distinguish contaminates from the mycelium. Guess i just have to take the plunge and start going at it.


any LC teks or good reads yall recommend?


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InvisibleMad Season
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Re: AGAR questions [Re: firstTIMER420]
    #21903207 - 07/05/15 10:42 PM (8 years, 6 months ago)

I just use 2g LME every 100 ml of water for lcs. Best way to test an lc is to drop it on a few plates of agar. If they all are colonized clean, you have VERY high chance the lc is clean. That's why I always say lc and agar goes hand in hand. You need agar to both inoculate and test its cleanliness.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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Re: AGAR questions [Re: firstTIMER420]
    #21903229 - 07/05/15 10:47 PM (8 years, 6 months ago)

Start with agar. It's the way to learn how to identify contams, understand what culture should look like and teaches sterile techniques. LC is what you do after you master agar.


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InvisibleMad Season
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Re: AGAR questions [Re: Pastywhyte]
    #21903244 - 07/05/15 10:51 PM (8 years, 6 months ago)

:whathesaid::bow2:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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