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Offlineroughneckn
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g2g
    #21893863 - 07/03/15 06:30 PM (8 years, 6 months ago)

Have a question.. I did a g2g transfer about 2 weeks ago an there is no change in progession. .any clues in why no growth?


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InvisibleMad Season
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Re: g2g [Re: roughneckn]
    #21893898 - 07/03/15 06:38 PM (8 years, 6 months ago)

:deathstar::notsureif:

Or fully colonized.. :shrug: but if not give it a wiff. Prepare to barf.


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Edited by Mad Season (07/03/15 06:40 PM)


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InvisibleMr. Alien
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Re: g2g [Re: roughneckn]
    #21893925 - 07/03/15 06:44 PM (8 years, 6 months ago)

Quote:

roughneckn said:
Have a question.. I did a g2g transfer about 2 weeks ago an there is no change in progession. .any clues in why no growth?




Bacteria or lack of GE?


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Offlineroughneckn
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Re: g2g [Re: Mr. Alien]
    #21893980 - 07/03/15 06:56 PM (8 years, 6 months ago)

Quote:

Mr. Alien said:
Quote:

roughneckn said:
Have a question.. I did a g2g transfer about 2 weeks ago an there is no change in progession. .any clues in why no growth?




Bacteria or lack of GE?


What's GE?


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Offlinenooberst
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Re: g2g [Re: roughneckn]
    #21893995 - 07/03/15 06:58 PM (8 years, 6 months ago)

Gas exchange


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Offlineroughneckn
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Re: g2g [Re: Mad Season]
    #21894011 - 07/03/15 06:59 PM (8 years, 6 months ago)

Quote:

Mad Season said:
:deathstar::notsureif:

Or fully colonized.. :shrug: but if not give it a wiff. Prepare to barf.




they were fully colonized for sure!!! I did it in my bedroom .. lysol air quick transfer then lip on...  used alcohol to wipe things down...


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InvisibleSpitballJedi
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Re: g2g [Re: roughneckn]
    #21894017 - 07/03/15 07:01 PM (8 years, 6 months ago)

If you did it in open air, you probably have a contamination. Pics of the jars would be helpful


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Offlineroughneckn
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Re: g2g [Re: roughneckn]
    #21894057 - 07/03/15 07:11 PM (8 years, 6 months ago)

Just opened one an it had a sweet smell to it


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Offlineroughneckn
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Re: g2g [Re: roughneckn]
    #21894101 - 07/03/15 07:20 PM (8 years, 6 months ago)



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InvisibleMr. Alien
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Re: g2g [Re: roughneckn]
    #21894122 - 07/03/15 07:24 PM (8 years, 6 months ago)

sweat smell = bacteria. Do you even have gas exchange holes?


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Offlineroughneckn
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Re: g2g [Re: Mr. Alien]
    #21894247 - 07/03/15 07:53 PM (8 years, 6 months ago)

No I don't didn't
Quote:

Mr. Alien said:
sweat smell = bacteria. Do you even have gas exchange holes?



Quote:

Mr. Alien said:
sweat smell = bacteria. Do you even have gas exchange holes?




no I don't didn't even know anything about gas exchange holes.. well there explains it all.. can you send me a tek to more info..


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OfflineGr0wer
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Re: g2g [Re: roughneckn]
    #21894264 - 07/03/15 07:56 PM (8 years, 6 months ago)

drill 1/4 hole stuff with polyfill is the best beginner tek....


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InvisibleMad Season
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Re: g2g [Re: Gr0wer]
    #21894372 - 07/03/15 08:24 PM (8 years, 6 months ago)

How did you have a fully colonized master jar without gas exchange holes?


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Offlineroughneckn
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Re: g2g [Re: Mad Season]
    #21894719 - 07/03/15 09:43 PM (8 years, 6 months ago)

Quote:

Mad Season said:
How did you have a fully colonized master jar without gas exchange holes?




We I didn't do enough reading...  my jars that I inoculate 6 total had 1/4" holes with poly...

Then I barely started reading on g2g transfer so when they were fully colonized I made mono tub with 4 of them .. then the other two I uses as master jars.. all I thought was to scoop in to presterile wbs with lid an that was it didn't even know you had to have holes on g2g transfer ..show you how much reading I did..


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InvisibleMr. Alien
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Re: g2g [Re: roughneckn]
    #21895526 - 07/04/15 03:45 AM (8 years, 6 months ago)

Quote:

roughneckn said:
Quote:

Mad Season said:
How did you have a fully colonized master jar without gas exchange holes?




We I didn't do enough reading...  my jars that I inoculate 6 total had 1/4" holes with poly...

Then I barely started reading on g2g transfer so when they were fully colonized I made mono tub with 4 of them .. then the other two I uses as master jars.. all I thought was to scoop in to presterile wbs with lid an that was it didn't even know you had to have holes on g2g transfer ..show you how much reading I did..




It wasn't only the lack of GE holes in your receiving jars, it was also your sterile procedures, g2g in open is super risky.


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Re: g2g [Re: Mr. Alien]
    #21895742 - 07/04/15 07:00 AM (8 years, 6 months ago)

One master jar you can knock another 7 and more if you want but 7 is good.
and you should have made the 1/4" holes with poly.


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Offlinemushpunx
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Re: g2g [Re: oontribe]
    #21895899 - 07/04/15 08:21 AM (8 years, 6 months ago)

Oh man yea I did my first G2G in open air and used a spoon :facepalm:

I "sterilized" the spoon with alchol haha.


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OfflineGr0wer
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Re: g2g [Re: mushpunx]
    #21896454 - 07/04/15 12:01 PM (8 years, 6 months ago)

Yea i used to use a spoon too. I would PC it wrapped in foil. Now i just shake the master jar before opening and pour directly into the jars being inoculated. KISS


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Invisibleoontribe
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Re: g2g [Re: Gr0wer]
    #21896489 - 07/04/15 12:12 PM (8 years, 6 months ago)

But TCs advised against using spoon or any other tools.


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Offlinemushpunx
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Re: g2g [Re: oontribe]
    #21898718 - 07/04/15 09:48 PM (8 years, 6 months ago)

Quote:

oontribe said:
But TCs advised against using spoon or any other tools.





Yeah no matter how clean you get them it's still pretty solid vector for contamination, always best to poor


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