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neef
Old Noob



Registered: 08/06/12
Posts: 154
Last seen: 10 months, 1 day
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Cloneufc'szip lock clone tek works but have questions
#21892859 - 07/03/15 02:17 PM (8 years, 6 months ago) |
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Hello everyone! I recently used cloneufc's ziplock clone tek which worked with no problem. I cloned the biggest B+ specimen in one of the best looking clusters. Let it sit in the bag for a week then placed it in a Qt jar of popcorn (I know people on here dislike popcorn spawn but want to give it a try). The clone colonized the jar nicely. He had is at 100% colonization and I have come up with some questions. The black thing in the jar is the clone.
1) Can the clone be placed in a new jar? Or does it lose it water content? (sort of like a g2g transfer)
2) If I use this jar as a master to g2g do I take the clone piece out?
3) Will the piece of clone be ok if I mix this jar with others in a mono tub?
4) How's you day going?


-------------------- Never the smartest one in the group. So I keep quite and listen.
Edited by neef (07/04/15 08:25 AM)
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neef
Old Noob



Registered: 08/06/12
Posts: 154
Last seen: 10 months, 1 day
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Re: Pasty's zip lock clone tek works but have questions [Re: neef]
#21895613 - 07/04/15 04:48 AM (8 years, 6 months ago) |
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No one has any input?
-------------------- Never the smartest one in the group. So I keep quite and listen.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Pasty's zip lock clone tek works but have questions [Re: neef]
#21895648 - 07/04/15 05:34 AM (8 years, 6 months ago) |
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pasty's "zip loack clone tek"?
u left what in the bag for a week?
u got all kind of different textured growth in that jar there bud, so unless u shook it and its recovering, i would not use that for g2g, or mix it with other jars.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: Pasty's zip lock clone tek works but have questions [Re: blindingleaf]
#21895766 - 07/04/15 07:12 AM (8 years, 6 months ago) |
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Pasty's Ziploc clone tek is new to me as well.
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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tetherface
get in where you fit in



Registered: 10/05/14
Posts: 553
Loc: wild
Last seen: 6 years, 6 months
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Re: Pasty's zip lock clone tek works but have questions [Re: Grey]
#21895842 - 07/04/15 07:48 AM (8 years, 6 months ago) |
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i've heard of this before but i don't think it's from pasty OP correct me if i'm wrong but you took a biopsy of a stem then throw that into a plastic baggie until it gets fuzzy then transfer that to inoculate your grain? i guess it could work if your sterile procedure is down pat but it sounds like a shot in the dark as to whether you'll end up with a clean culture Happy 4th of July Shroomery!!!
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neef
Old Noob



Registered: 08/06/12
Posts: 154
Last seen: 10 months, 1 day
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Re: Pasty's zip lock clone tek works but have questions [Re: tetherface]
#21895901 - 07/04/15 08:22 AM (8 years, 6 months ago) |
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Quote:
tetherface said: i've heard of this before but i don't think it's from pasty OP correct me if i'm wrong but you took a biopsy of a stem then throw that into a plastic baggie until it gets fuzzy then transfer that to inoculate your grain? i guess it could work if your sterile procedure is down pat but it sounds like a shot in the dark as to whether you'll end up with a clean culture Happy 4th of July Shroomery!!!
I was wrong! It's cloneufc tek. Editing the first post now
-------------------- Never the smartest one in the group. So I keep quite and listen.
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neef
Old Noob



Registered: 08/06/12
Posts: 154
Last seen: 10 months, 1 day
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Re: Pasty's zip lock clone tek works but have questions [Re: blindingleaf]
#21895913 - 07/04/15 08:29 AM (8 years, 6 months ago) |
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Quote:
blindingleaf said: pasty's "zip loack clone tek"?
u left what in the bag for a week?
u got all kind of different textured growth in that jar there bud, so unless u shook it and its recovering, i would not use that for g2g, or mix it with other jars.
I left the piece of stem I cut off in the ziplock bag let it get fuzzy then placed in a qt jar. Sorry for the confusion
-------------------- Never the smartest one in the group. So I keep quite and listen.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Pasty's zip lock clone tek works but have questions [Re: neef]
#21895926 - 07/04/15 08:36 AM (8 years, 6 months ago) |
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u've probably got some kind of contam. u can clone that way, but its not reliable, especially for someone just getting into culture work.
give it a smell maybe. but i would not g2g that. u can try spawning it if u want. if ur grains were closer together, instead of in large clumps like u have, u could try to case and fruit. but it just doesn't look healthy to me.
look at "pastywhytes easy agar TEK" and try to do that next time you want to clone. its easier than u might think, and 99% more effective.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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neef
Old Noob



Registered: 08/06/12
Posts: 154
Last seen: 10 months, 1 day
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Re: Pasty's zip lock clone tek works but have questions [Re: blindingleaf]
#21896014 - 07/04/15 09:06 AM (8 years, 6 months ago) |
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Quote:
blindingleaf said: u've probably got some kind of contam. u can clone that way, but its not reliable, especially for someone just getting into culture work.
give it a smell maybe. but i would not g2g that. u can try spawning it if u want. if ur grains were closer together, instead of in large clumps like u have, u could try to case and fruit. but it just doesn't look healthy to me.
look at "pastywhytes easy agar TEK" and try to do that next time you want to clone. its easier than u might think, and 99% more effective.
Smells like fresh mushrooms. Looks very white. Are you saying not to g2g due to the fact you think it's contam? Is it possible the contam wouldn't show till being spawn?
-------------------- Never the smartest one in the group. So I keep quite and listen.
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