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PutACapInHisAss
Stranger Than Fiction



Registered: 08/11/07
Posts: 252
Loc: Earth
Last seen: 6 years, 4 months
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To Shake or Not to Shake a Multispore Inoculation?
#21892522 - 07/03/15 12:46 PM (8 years, 6 months ago) |
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Should I shake up my jar after an inoculation or leave it be until I have enough mycelium growth for a jiggle? I found some threads about this but they are quite old--just wondering what people are doing these days.
Things always change. Last time I was in the hobby some people were still bottom casing
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Th3Issu3
Stranger Danger


Registered: 12/30/14
Posts: 671
Loc: fucktown
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Re: To Shake or Not to Shake a Multispore Inoculation? [Re: PutACapInHisAss]
#21892536 - 07/03/15 12:50 PM (8 years, 6 months ago) |
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With grain or WBS, Inocc than give a shake....After its about 30 % give another shake. Then when you are ready to spawn to bulk shake it up again.
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: To Shake or Not to Shake a Multispore Inoculation? [Re: Th3Issu3]
#21892594 - 07/03/15 01:07 PM (8 years, 6 months ago) |
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Don't shake when inoculating with spores. that just slows germination by spreading the spores away from each other. wait for 20-30% then shake.
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Th3Issu3
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Re: To Shake or Not to Shake a Multispore Inoculation? [Re: MudaFuka]
#21892620 - 07/03/15 01:13 PM (8 years, 6 months ago) |
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Quote:
MudaFuka said: Don't shake when inoculating with spores. that just slows germination by spreading the spores away from each other. wait for 20-30% then shake.
I've always shook them, not going to next time i work with a MS syringe. Thanks
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: To Shake or Not to Shake a Multispore Inoculation? [Re: Th3Issu3]
#21892623 - 07/03/15 01:14 PM (8 years, 6 months ago) |
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I used to shake them too.
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E go death
Knowledge Seeker



Registered: 03/16/15
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Loc: Oregon
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Re: To Shake or Not to Shake a Multispore Inoculation? [Re: MudaFuka]
#21892651 - 07/03/15 01:19 PM (8 years, 6 months ago) |
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Do not shake your jars after inoculating with spores. Like MudaFuka said, this is counter productive.
-------------------- We are the music makers... And we are the dreamers of the dreams.
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Green Bastard
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Registered: 02/26/14
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Re: To Shake or Not to Shake a Multispore Inoculation? [Re: MudaFuka]
#21892664 - 07/03/15 01:23 PM (8 years, 6 months ago) |
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There was a big discussion on this last week. Consensus is don't shake MS until 30%, unless you're using too much of your syringe liquid. I shake LC inoculations right away, and then leave them go to 100%...
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Toadstool5
A Registered Mycophile



Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
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Re: To Shake or Not to Shake a Multispore Inoculation? [Re: Green Bastard]
#21897918 - 07/04/15 06:05 PM (8 years, 6 months ago) |
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Quote:
Don't shake when inoculating with spores. that just slows germination by spreading the spores away from each other. wait for 20-30% then shake.
Quote:
unless you're using too much of your syringe liquid
If you used a normal amount of spores then dont shake them or the chances of them finding a compatible match for dikaryotic growth will diminish.
If you used A LOT of spores then by all means shake it up!
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: To Shake or Not to Shake a Multispore Inoculation? [Re: Toadstool5] 1
#21897941 - 07/04/15 06:10 PM (8 years, 6 months ago) |
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we always use alot of s[ores though....thousands lol, there is no need to shake and if you do not only is what is said above true but you also make it harder to judge when it's 30% and spot contams from the syringe
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Toadstool5
A Registered Mycophile



Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
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Re: To Shake or Not to Shake a Multispore Inoculation? [Re: cronicr]
#21898534 - 07/04/15 09:09 PM (8 years, 6 months ago) |
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Thays why i said A LOT 
You would have to use an entire print in a 10ml to have any reason to shake
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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