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OfflinePutACapInHisAss
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To Shake or Not to Shake a Multispore Inoculation?
    #21892522 - 07/03/15 12:46 PM (8 years, 6 months ago)

Should I shake up my jar after an inoculation or leave it be until I have enough mycelium growth for a jiggle? I found some threads about this but they are quite old--just wondering what people are doing these days.

Things always change. Last time I was in the hobby some people were still bottom casing :smile:


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OfflineTh3Issu3
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Re: To Shake or Not to Shake a Multispore Inoculation? [Re: PutACapInHisAss]
    #21892536 - 07/03/15 12:50 PM (8 years, 6 months ago)

With grain or WBS, Inocc than give a shake....After its about 30 % give another shake. Then when you are ready to spawn to bulk shake it up again.


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InvisibleMudaFuka
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Re: To Shake or Not to Shake a Multispore Inoculation? [Re: Th3Issu3]
    #21892594 - 07/03/15 01:07 PM (8 years, 6 months ago)

Don't shake when inoculating with spores. that just slows germination by spreading the spores away from each other.  wait for 20-30% then shake.


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OfflineTh3Issu3
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Re: To Shake or Not to Shake a Multispore Inoculation? [Re: MudaFuka]
    #21892620 - 07/03/15 01:13 PM (8 years, 6 months ago)

Quote:

MudaFuka said:
Don't shake when inoculating with spores. that just slows germination by spreading the spores away from each other.  wait for 20-30% then shake.





I've always shook them, not going to next time i work with a MS syringe. Thanks


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Re: To Shake or Not to Shake a Multispore Inoculation? [Re: Th3Issu3]
    #21892623 - 07/03/15 01:14 PM (8 years, 6 months ago)

:thumbup: I used to shake them too.


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OfflineE go death
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Re: To Shake or Not to Shake a Multispore Inoculation? [Re: MudaFuka]
    #21892651 - 07/03/15 01:19 PM (8 years, 6 months ago)

Do not shake your jars after inoculating with spores. Like MudaFuka said, this is counter productive.


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OfflineGreen Bastard
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Re: To Shake or Not to Shake a Multispore Inoculation? [Re: MudaFuka]
    #21892664 - 07/03/15 01:23 PM (8 years, 6 months ago)

There was a big discussion on this last week. Consensus is don't shake MS until 30%, unless you're using too much of your syringe liquid. I shake LC inoculations right away, and then leave them go to 100%...


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InvisibleToadstool5
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Re: To Shake or Not to Shake a Multispore Inoculation? [Re: Green Bastard]
    #21897918 - 07/04/15 06:05 PM (8 years, 6 months ago)

Quote:

Don't shake when inoculating with spores. that just slows germination by spreading the spores away from each other.  wait for 20-30% then shake.




Quote:

unless you're using too much of your syringe liquid




If you used a normal amount of spores then dont shake them or the chances of them finding a compatible match for dikaryotic growth will diminish.

If you used A LOT of spores then by all means shake it up! :rofl:


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Re: To Shake or Not to Shake a Multispore Inoculation? [Re: Toadstool5] * 1
    #21897941 - 07/04/15 06:10 PM (8 years, 6 months ago)

we always use alot of s[ores though....thousands lol, there is no need to shake  and if you do not only is what is said above true but you also make it harder to judge when it's 30% and spot contams from the syringe


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InvisibleToadstool5
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Re: To Shake or Not to Shake a Multispore Inoculation? [Re: cronicr]
    #21898534 - 07/04/15 09:09 PM (8 years, 6 months ago)

Thays why i said  A LOT :rofl:

You would have to use an entire print in a 10ml to have any reason to shake :lol:


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