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InvisibleAlexTheFunguy
Explorer


Registered: 07/04/11
Posts: 40
Loc: Holland
MS syringe vs LC myc inoculation
    #21891899 - 07/03/15 09:43 AM (8 years, 6 months ago)

is there a difference between MS inoculation and LC inoculation? I'm talkin about like the differences between MS and agar. I've done Done both and I've noticed how LC is better(in lack of agar) for colonizing (grain and sub) and fruiting. I was just wondering if by growing the spores outside the grains and/or substrate before introducing them to what they will munch on acts similar to how they would perform thru the agar process.


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OfflineMonkeyJesusFresco
am i suspended in agar?
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Registered: 10/09/12
Posts: 3,306
Loc: South East USA Flag
Last seen: 7 hours, 54 minutes
Re: MS syringe vs LC myc inoculation [Re: AlexTheFunguy]
    #21891940 - 07/03/15 10:00 AM (8 years, 6 months ago)

There's no difference. Other than LC is already alive and thriving, MS will have to germinate.


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InvisibleAlexTheFunguy
Explorer


Registered: 07/04/11
Posts: 40
Loc: Holland
Re: MS syringe vs LC myc inoculation [Re: MonkeyJesusFresco]
    #21891959 - 07/03/15 10:07 AM (8 years, 6 months ago)

Oh ok. Yea I guess just seemed different to me


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InvisibleMaxwell smart
Special agent
Registered: 06/28/15
Posts: 41
Loc: Mother ship
Re: MS syringe vs LC myc inoculation [Re: AlexTheFunguy]
    #21892091 - 07/03/15 10:52 AM (8 years, 6 months ago)

Lc will give you the quicker results. And the faster it grows the faster it can fight contamination.
Pluse you can make a gal of lc for like $1.34. So its cheaper and last longer


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InvisibleMad Season
hookers and blackjack
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Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: MS syringe vs LC myc inoculation [Re: Maxwell smart]
    #21892113 - 07/03/15 10:58 AM (8 years, 6 months ago)

I wouldn't trust lc by itself. It needs agar to compliment it. Syringe> agar. 1 clean transfer to get rid of contams, agar> lc to ensure a clean inoculation of the lc, and then test the lc on a few dishes of agar to ensure its clean. That costs a little more than 2$. But it will seriously save in costs of other materials because you won't have to throw everything away due to contamination.


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OfflineShroominMe
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Registered: 05/03/15
Posts: 525
Last seen: 8 years, 3 months
Re: MS syringe vs LC myc inoculation [Re: Maxwell smart]
    #21893711 - 07/03/15 05:48 PM (8 years, 6 months ago)

LC has a bigger upside but also a greater tendency to contam.  That's why there are lots of folks who swear by agar - you can see any potential contam before you colonize on grain.

With one spore syringe you can inoculate at least 8 quart jars and 4 LCs.  Might be good to spread the love around, especially if you're inexperienced.


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