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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: Mad Season's sterile techniques: Inoculation [Re: taGyo]
#22720249 - 12/31/15 09:48 AM (8 years, 1 month ago) |
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-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Mad Season's sterile techniques: Inoculation [Re: taGyo]
#22720347 - 12/31/15 10:14 AM (8 years, 1 month ago) |
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I really need to get around to updating this
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: Mad Season's sterile techniques: Inoculation [Re: Mad Season]
#22745172 - 01/06/16 06:01 PM (8 years, 24 days ago) |
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-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Mad Season's sterile techniques: Inoculation [Re: taGyo]
#22870967 - 02/06/16 12:00 PM (7 years, 11 months ago) |
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Bumping this to help people improve their sterile techniques
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Psilosoulful
Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Mad Season's sterile techniques: Inoculation [Re: Mad Season]
#22922818 - 02/19/16 11:47 AM (7 years, 11 months ago) |
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TravelAgency
The ongoing "wow"


Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 22 days
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Re: Mad Season's sterile techniques: Inoculation [Re: Psilosoulful]
#22925173 - 02/20/16 12:18 AM (7 years, 11 months ago) |
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Wow, it's crazy that 9 months ago I hadn't meddled in agar yet. This post helped me a lot, and now agar is the norm for me. Between Pasty and You, I have learned so much about agar and how to use it and it has changed how I do mycology forever. Newbies read this please.
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Psilosoulful
Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Mad Season's sterile techniques: Inoculation [Re: TravelAgency]
#22925205 - 02/20/16 12:28 AM (7 years, 11 months ago) |
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Mad Season's sterile techniques: Inoculation [Re: Psilosoulful]
#22925867 - 02/20/16 09:06 AM (7 years, 11 months ago) |
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Agar is killer stuff and fun as well. Glad yer on board
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Mad Season's sterile techniques: Inoculation [Re: Pastywhyte]
#22926059 - 02/20/16 10:27 AM (7 years, 11 months ago) |
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PsilocyBen17
Pin Pornographer


Registered: 10/20/13
Posts: 3,751
Last seen: 1 year, 1 month
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Re: Mad Season's sterile techniques: Inoculation [Re: Mad Season]
#22986961 - 03/08/16 07:47 PM (7 years, 10 months ago) |
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Thanks for the pictoral Mad Season. I found it helpful.
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Will7401


Registered: 01/27/16
Posts: 154
Last seen: 13 days, 7 hours
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Re: Mad Season's sterile techniques: Inoculation [Re: Pastywhyte]
#23134117 - 04/19/16 01:08 PM (7 years, 9 months ago) |
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Quote:
Pastywhyte said: Nice writeup Mad! Agar is not hard at all is it
My advice to anyone just staring out in this field...research and practice with agar before wasting any time or money with any other method. Agar IMNO (in my newbie opinion) is the best method regardless of how daunting it may appear at first. it really isn't that difficult and making mistakes are very cheap compared to, oh I don't know, say going through two whole 12 cc MS of Cambodia and eventually feeding the birds and squirrels 20 jars of rye grain. Pasty you have been a champ in my corner so far and Mad Season, you just helped me tremendously with a couple of things i see that I had been doing wrong. Namely, when I placed a drop or two of MS on the agar I tried to be as careful as possible to not move it around thinking this would help it germinate faster by keeping the spores all together. From here on out I will definitely try to disperse them now. Makes total sense too. I'm glad to know it's okay to use the side of the jar to get the wedge off my scalpel. I am having a rather difficult time getting them to come off whether it be agar to agar transfer or agar to grain. They're very sticky. A heated coffee can huh? Don't I feel like a dumbass. This is a kickass write up brother. Thank you.
-------------------- Love and Light to us all. Namaste
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: Mad Season's sterile techniques: Inoculation [Re: Will7401]
#23659240 - 09/19/16 04:11 PM (7 years, 4 months ago) |
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A bunch of people need to see this. I read it every couple of months  Thanks for taking the time to put this together, Mad.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Mad Season's sterile techniques: Inoculation [Re: dankington]
#23659613 - 09/19/16 06:17 PM (7 years, 4 months ago) |
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Lol it's been 6 months since this was bumped? Wow time flies
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Chips
Stranger

Registered: 01/05/16
Posts: 32
Last seen: 5 years, 2 months
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Re: Mad Season's sterile techniques: Inoculation [Re: Mad Season]
#23909203 - 12/09/16 05:52 AM (7 years, 1 month ago) |
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thank you for this tek, the tip about cutting into the receiving plate with the agar made my work so much cleaner, just a simple cut and drag instead of scraping the scalpel around everywhere trying to get the wedge off 
i hope it's okay to bump this, i have a SAB technique question
after prepping grain, i store it on a shelf in a closet. i leave the foil covering the top
i wipe down the jars (including the foil) before bringing them in to the SAB
after removing the foil inside the SAB, do i need to wipe off the lid before opening for inoculation? or is the foil sufficient enough to keep it safe?
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Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 33 minutes, 1 second
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Re: Mad Season's sterile techniques: Inoculation [Re: Chips]
#23909325 - 12/09/16 07:20 AM (7 years, 1 month ago) |
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Just recently did my first agar plates... Inoculated 18 of them with various species and strains. I hope I was sterile enough, I think I might have been too impatient and rushed. We'll see. This is nerve wracking, lol.
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Mad Season's sterile techniques: Inoculation [Re: Chips]
#23909381 - 12/09/16 07:51 AM (7 years, 1 month ago) |
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Quote:
Chips said: thank you for this tek, the tip about cutting into the receiving plate with the agar made my work so much cleaner, just a simple cut and drag instead of scraping the scalpel around everywhere trying to get the wedge off 
i hope it's okay to bump this, i have a SAB technique question
after prepping grain, i store it on a shelf in a closet. i leave the foil covering the top
i wipe down the jars (including the foil) before bringing them in to the SAB
after removing the foil inside the SAB, do i need to wipe off the lid before opening for inoculation? or is the foil sufficient enough to keep it safe?
Foil should be fine. Realistically if you did things right and only had the sterile lid bottoms on top of the open media, you don't even need to wipe things down. Just don't let anything but the underside of the lid go over it. Alcohol is more of a safety net than a necessity IMO.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Mad Season's sterile techniques: Inoculation [Re: Mad Season]
#23909702 - 12/09/16 10:27 AM (7 years, 1 month ago) |
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-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Chips
Stranger

Registered: 01/05/16
Posts: 32
Last seen: 5 years, 2 months
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Re: Mad Season's sterile techniques: Inoculation [Re: Mad Season]
#23911656 - 12/09/16 09:56 PM (7 years, 1 month ago) |
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Quote:
Mad Season said:
Quote:
Chips said: thank you for this tek, the tip about cutting into the receiving plate with the agar made my work so much cleaner, just a simple cut and drag instead of scraping the scalpel around everywhere trying to get the wedge off 
i hope it's okay to bump this, i have a SAB technique question
after prepping grain, i store it on a shelf in a closet. i leave the foil covering the top
i wipe down the jars (including the foil) before bringing them in to the SAB
after removing the foil inside the SAB, do i need to wipe off the lid before opening for inoculation? or is the foil sufficient enough to keep it safe?
Foil should be fine. Realistically if you did things right and only had the sterile lid bottoms on top of the open media, you don't even need to wipe things down. Just don't let anything but the underside of the lid go over it. Alcohol is more of a safety net than a necessity IMO.

it took me 2 hours to noc 24 jars the other night because i was meticulously wiping every jar before it went in and after i removed the foil lol
this makes a lot more sense, ty!
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NothingsChanged
Striving for Excellence



Registered: 05/28/11
Posts: 10,146
Loc: North/Western WA
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Re: Mad Season's sterile techniques: Inoculation [Re: Chips]
#24224343 - 04/07/17 12:15 AM (6 years, 9 months ago) |
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Great tek. Helped tremendously.
--------------------

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germanauslander
Forever Learner
Registered: 11/06/16
Posts: 100
Last seen: 5 years, 6 months
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Re: Improve your sterile techniques! (a comprehensive guide to agar) [Re: Mad Season]
#24224406 - 04/07/17 12:48 AM (6 years, 9 months ago) |
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