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Offlinebennylava
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Registered: 05/29/15
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Re: Mad Season's sterile techniques: Inoculation [Re: Mad Season]
    #22313670 - 09/30/15 11:59 AM (8 years, 3 months ago)

When mycelium first appears, can it have an ever so slight, opaque look to it? I know its supposed to be sort of a bright white, and it looks like it sort of is, but if I look closely, its just a tiny bit... see through. Looks like if it were much larger, you just might be able to see through it, in the very center. But to be more clear, this piece of suspected mycelium, is only about 1/5 the size of a BB. And it is round.


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InvisibleMad Season
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Re: Mad Season's sterile techniques: Inoculation [Re: bennylava]
    #22313729 - 09/30/15 12:09 PM (8 years, 3 months ago)

Let it grow out some. Wait for any visible rizmorphs. Usually has to get to the size of a dime. Show some pics if you can.


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Offlinebennylava
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Re: Mad Season's sterile techniques: Inoculation [Re: Mad Season]
    #22313968 - 09/30/15 12:57 PM (8 years, 3 months ago)

Quote:

Mad Season said:
Let it grow out some. Wait for any visible rizmorphs. Usually has to get to the size of a dime. Show some pics if you can.





Ok I'll post a pic or two, once its of a significant size that will be big enough for pics. Two more questions:

1. Is it ever ok to open the lids of your jars or petri dishes? Is this ever done?

2. Self healing silicon inoculation ports seem to be all the rage these days. Is this really necessary? Or is it more for people who suffer from frequent contamination? Some people don't practice very much sterile technique, and they don't seem to have too many problems. I mean they'll do the basics, but they don't go the extra mile. And other people, seem to want damn near a cleanroom or a filtered sterile lab to work in. So that makes me wonder, if these "self healing" ports are really even that helpful/important.


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InvisibleMad Season
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Re: Mad Season's sterile techniques: Inoculation [Re: bennylava]
    #22314003 - 09/30/15 01:02 PM (8 years, 3 months ago)

I don't have any ships. As you can see in the tek i just pop the lids and go to town haha.

As for if you can open the lid to take a pic.. I really don't recommend it. If anything. Take a pic after you've transfered.


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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Offlinebennylava
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Re: Mad Season's sterile techniques: Inoculation [Re: Mad Season]
    #22314038 - 09/30/15 01:09 PM (8 years, 3 months ago)

Quote:

Mad Season said:
I don't have any ships. As you can see in the tek i just pop the lids and go to town haha.

As for if you can open the lid to take a pic.. I really don't recommend it. If anything. Take a pic after you've transfered.




Ok, say say someone had a contamination, but they went ahead and grew everything to full fruition. Harvested the mushrooms, and ate them. In what manner, could the contaminants hurt the person? Is there mold on the mushroom itself, and the person eats the mold? Can someone break down how this goes down? Just trying to understand, I don't have any intention of eating shrooms grown from a contaminated jar.


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InvisibleMad Season
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Re: Mad Season's sterile techniques: Inoculation [Re: bennylava]
    #22314058 - 09/30/15 01:13 PM (8 years, 3 months ago)

The person who eats it will be fine. No one has gone to the hospital from eating a fruit from a contaminated substrate. Ever. Every mushroom picked in the wild has a substrate with some sort of contamination. Any visible molds on fruit bodies I'd stay away from tho.


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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Offlinebennylava
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Re: Mad Season's sterile techniques: Inoculation [Re: Mad Season]
    #22314107 - 09/30/15 01:25 PM (8 years, 3 months ago)

Quote:

Mad Season said:
The person who eats it will be fine. No one has gone to the hospital from eating a fruit from a contaminated substrate. Ever. Every mushroom picked in the wild has a substrate with some sort of contamination. Any visible molds on fruit bodies I'd stay away from tho.




So the fight against contamination, is just so that the mushrooms will ever grow. That sound about right?


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InvisibleMad Season
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Re: Mad Season's sterile techniques: Inoculation [Re: bennylava]
    #22314552 - 09/30/15 02:49 PM (8 years, 3 months ago)

I'm not sure. But it applies to every mushroom species. Including edibles. Mushrooms/molds will compete against each other, and most mushrooms will just contain contaminations to stop from growing. They don't draw up any toxins into the fruiting bodies tho


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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Offlinebennylava
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Re: Mad Season's sterile techniques: Inoculation [Re: Mad Season]
    #22317862 - 10/01/15 08:26 AM (8 years, 3 months ago)

Well it looks like my little white dots have gained some little friends. They're orange, or perhaps a bit of a brownish orange. Growing about a half inch away from the white dots. But only in a couple of my petri dishes, not all.

If the white dots turn out to actually be mycelium, and some other contaminants are growing in there with them, can I just cut some of the myc out of there, and just use that to colonize a different dish? Or even a grain jar?


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InvisibleMad Season
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Re: Mad Season's sterile techniques: Inoculation [Re: bennylava]
    #22318035 - 10/01/15 09:12 AM (8 years, 3 months ago)

A new dish. Never put it in a grain jar until the whole dish gets almost colonized completely


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Offlinebennylava
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Re: Mad Season's sterile techniques: Inoculation [Re: Mad Season]
    #22322105 - 10/02/15 03:43 AM (8 years, 3 months ago)

And now a question that's a bit more broad and general.

Do you think that most people, once they get a good colony a mycelium going, just use that to colonize new stuff? Maybe they only use a spore syringe once. Sure they might make some spore prints just so they'll always have a source of spores in case anything happens, but they really don't use spore prints or syringes anymore. They just draw from their already growing colony of mycelium. After all, why wait for the mycelium to appear, when you already have some on hand, and you can just put a little of that, in your jar or whatever you're wanting to grow in.

Is this how its generally done, to save some time?


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Invisibleblindingleaf
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Re: Mad Season's sterile techniques: Inoculation [Re: bennylava]
    #22322135 - 10/02/15 04:34 AM (8 years, 3 months ago)

most people will save clones/isolates, and pull from that if its good genetics.
once u get used to germinating spores, its like anything else though, u just kinda assume its going to take a week or so to see growth.
i'd guess most regulars on here have several things going at once, so if one thing is taking longer, they might not even notice it because other things are occupying their time.


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Offlinebennylava
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Re: Mad Season's sterile techniques: Inoculation [Re: blindingleaf]
    #22322187 - 10/02/15 05:25 AM (8 years, 3 months ago)

Quote:

blindingleaf said:
most people will save clones/isolates, and pull from that if its good genetics.
once u get used to germinating spores, its like anything else though, u just kinda assume its going to take a week or so to see growth.
i'd guess most regulars on here have several things going at once, so if one thing is taking longer, they might not even notice it because other things are occupying their time.




What would make the genetics bad? I'm assuming that the people selling the spores, are high end "users". And that they spores they're selling, are from the best stock.


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Invisibleblindingleaf
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Re: Mad Season's sterile techniques: Inoculation [Re: bennylava]
    #22322210 - 10/02/15 05:42 AM (8 years, 3 months ago)

two harvard graduates can still have retard babies right?

u start over from spores, although some tendencies can carry over as you go further into print>grow>print>grow, etc. 

but as a general rule, as growers, we assume that when we do MS grows, we have same chance for awesome flushes and fruits as we do for not so good flushes and fruits because we can't be exactly sure what kinds of genetic expression will occur when hundreds of spore matings have taken place.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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OfflineBobby2fish
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Re: Mad Season's sterile techniques: Inoculation [Re: Psilosoulful]
    #22444506 - 10/28/15 11:39 AM (8 years, 3 months ago)

Very understandable and the tip you gave of NOT allowing your hand to go over the open container is a good one. Thank You


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OfflineBobby2fish
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Re: Mad Season's sterile techniques: Inoculation [Re: bennylava]
    #22444543 - 10/28/15 11:52 AM (8 years, 3 months ago)

I highly doubt You will ever get 100 % NO contams for 7 consecutive years and I bet if you tried anyone's tek for 7 years you will get a few  jars full of clean Mycelium, so what is possible lies somewhere in the middle.
Do all you can to eliminate contaminated air around your grow and You will do better.


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Offlinespacechildo
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Re: Mad Season's sterile techniques: Inoculation [Re: Mad Season]
    #22444601 - 10/28/15 12:06 PM (8 years, 3 months ago)

I was sure I'd already posted in this thread, great write up Mad! :hatsoff:


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Offlineinvitro

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Re: Mad Season's sterile techniques: Inoculation [Re: spacechildo]
    #22444722 - 10/28/15 12:42 PM (8 years, 3 months ago)

:takingnotes:


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InvisibleMad Season
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Re: Improve your sterile techniques! (a comprehensive guide to agar) [Re: Mad Season]
    #22710722 - 12/28/15 08:42 PM (8 years, 1 month ago)

:snort::bump:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflinetaGyo
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Re: Improve your sterile techniques! (a comprehensive guide to agar) [Re: Mad Season]
    #22710886 - 12/28/15 09:32 PM (8 years, 1 month ago)

:tongue2:


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