Home | Community | Message Board

MushroomMan Mycology
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: < Back | 1 | 2 | 3 | 4 | 5 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineMagicInMichigan
Internal conquistador
Male


Registered: 07/07/15
Posts: 138
Loc: Michigan,U.S.A.
Last seen: 8 years, 2 months
Re: Mad Season's sterile techniques: Inoculation [Re: taGyo]
    #22007082 - 07/27/15 09:53 PM (8 years, 6 months ago)

Good shit dude. Did my agar innoc from your EQ&Lipa Yai prints and saralove's P. mexicana CN using basically this exact sterile Tek today except from prints, very helpful. My transfers from the dirty old Chilean print looked clean last time around finally so hopefully this will be the last transfer for them today too, super fast growth& very rhizo :smile: shit basically covers a plate in 5 days  since every transfer after the second. Kept taking the fastest sector every time, now there is no stopping it. Can't wait to see what it does to some grain jars. Thanks for writing this up, I know a lot of people probaly have this stuff up else ware but this one was way easy to follow.


-MIM


--------------------
β€œthe only people for me are the mad ones, the ones who are mad to live, mad to talk, mad to be saved, desirous of everything at the same time, the ones who never yawn or say a commonplace thing, but burn, burn, burn like fabulous yellow roman candles exploding like spiders across the stars.”

I surround myself with people who aren't afraid to live. They have had what they love most taken from them, be it freedom, love, money, or anything for that matter. When you lose your greatest love you also lose your greatest fear, when that happens you are completely and utterly free.


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Mad Season's sterile techniques: Inoculation [Re: MagicInMichigan]
    #22007210 - 07/27/15 10:25 PM (8 years, 6 months ago)

:blush: you guys are too kind! I'm glad you find it easy to follow. I was trying to be as thorough as I could. There are a bunch of one's I've read. I just wanted to share what techniques I developed to get my success


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
Offlineuninc4life2010
Unincorporated
Male User Gallery


Registered: 06/05/10
Posts: 1,124
Last seen: 7 months, 12 days
Re: Mad Season's sterile techniques: Inoculation [Re: Mad Season]
    #22007671 - 07/28/15 12:47 AM (8 years, 6 months ago)

After I transfer mycelium to a new agar plate, is it necessary to wrap the dish in parafilm while still in the SAB to ensure that no contams get in once it is removed and stored?


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Mad Season's sterile techniques: Inoculation [Re: uninc4life2010]
    #22007710 - 07/28/15 01:03 AM (8 years, 6 months ago)

Yessir! Generally only with Petris. Not pasty plates, which should have filtered gas exchange. Lots of people use saran wrap instead. It's a way cheaper option.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (07/28/15 01:22 AM)


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: Mad Season's sterile techniques: Inoculation [Re: Mad Season] * 1
    #22248188 - 09/16/15 09:00 PM (8 years, 4 months ago)

:bump2: Great post


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
Invisiblenatedawgnow
Rocky mountain hood rat
 User Gallery


Registered: 02/09/15
Posts: 8,939
Loc: ation
Trusted Cultivator
Re: Mad Season's sterile techniques: Inoculation [Re: cronicr]
    #22248799 - 09/16/15 11:07 PM (8 years, 4 months ago)

Good stuff, man!

Very informative!


--------------------


Extras: Filter Print Post Top
OfflinefirstTIMER420
Born the son of a sharecropper..
Male User Gallery

Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
Re: Mad Season's sterile techniques: Inoculation [Re: uninc4life2010]
    #22248911 - 09/16/15 11:49 PM (8 years, 4 months ago)

Quote:

uninc4life2010 said:
After I transfer mycelium to a new agar plate, is it necessary to wrap the dish in parafilm while still in the SAB to ensure that no contams get in once it is removed and stored?





Glad I looked at mads answer, I usually open my SAB and wrap them outside. Havent gotten any contams I can think of because of it, but I guess ill have to be more careful.

Also mad, how long do you wait till you take a transfer? I usually wait until about half the plate is covered with Myc so I can generally see how its going to grow/group out


--------------------
12 34 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url]
--Anything posted by this account is completely fictitious--


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Mad Season's sterile techniques: Inoculation [Re: firstTIMER420]
    #22250338 - 09/17/15 10:05 AM (8 years, 4 months ago)

Quote:

Mad Season said:
Transferring

First transfer (Agar2Agar)

You want to transfer basically a few days after you see growth. Like:





I like to transfer away from contams. I just go for thicker places with fast growth normally. I'd choose the bottom growth anywhere from 7-11 o'clock.

Afterwards if I'm just going to transfer again I'd like it to get to around this size:
(I'd stay away from the 6 o'clock area of the bottom growth)

I generally like to let them get almost to the edge before putting into grain jars.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
Offlinebennylava
Bad example


Registered: 05/29/15
Posts: 587
Last seen: 6 years, 9 months
Re: Mad Season's sterile techniques: Inoculation [Re: Mad Season]
    #22301188 - 09/28/15 01:39 AM (8 years, 4 months ago)

Can someone go into a little more depth, about the collars he said you could make, in that first video in the OP?

Right now, my SAB just has two holes. That's two holes for contams to drift right on in. I'd like to have some of these collars, that hold the gloves in place permanently. Is there some place that sells them? I'd like to avoid having to make them out of wood.


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Mad Season's sterile techniques: Inoculation [Re: bennylava]
    #22301564 - 09/28/15 07:15 AM (8 years, 4 months ago)

The pvc collars are so bleh to me. A still air box is much better than a glove box. Glove boxes are so 2003. You'll have a better success rate in a proper sab over a gb.

I mean make it with collars if you want. Just saying that it's not necessary.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
OfflinefirstTIMER420
Born the son of a sharecropper..
Male User Gallery


Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
Re: Mad Season's sterile techniques: Inoculation [Re: Mad Season]
    #22305993 - 09/28/15 11:49 PM (8 years, 4 months ago)

Quote:

Mad Season said:
Quote:

Mad Season said:
Transferring

First transfer (Agar2Agar)

You want to transfer basically a few days after you see growth. Like:





I like to transfer away from contams. I just go for thicker places with fast growth normally. I'd choose the bottom growth anywhere from 7-11 o'clock.

Afterwards if I'm just going to transfer again I'd like it to get to around this size:
(I'd stay away from the 6 o'clock area of the bottom growth)

I generally like to let them get almost to the edge before putting into grain jars.




Man, still cannot get my damn pan cyan to germinate...ehem.....

What the hell is a collar?

also, you place two transfers on 1 100mm plate?


--------------------
12 34 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url]
--Anything posted by this account is completely fictitious--


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: Mad Season's sterile techniques: Inoculation [Re: firstTIMER420]
    #22306062 - 09/29/15 12:22 AM (8 years, 4 months ago)

give it some time, if the plate is clean just leave it mine took 16 days to germinate(9 year old print though)


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
Offlinebennylava
Bad example


Registered: 05/29/15
Posts: 587
Last seen: 6 years, 9 months
Re: Mad Season's sterile techniques: Inoculation [Re: cronicr]
    #22306547 - 09/29/15 04:36 AM (8 years, 4 months ago)

What causes it to just stall out, and never take off? What are the causes of it just failing to germinate?


Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery

Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: Mad Season's sterile techniques: Inoculation [Re: bennylava] * 1
    #22306752 - 09/29/15 06:14 AM (8 years, 4 months ago)

inviable spores (usually old) or media is not soft/watery enough.
if u see germination, then it dies back, do not use.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


Extras: Filter Print Post Top
OfflineLogicaL ChaosM
Ascension Energy & Alien UFOs
Male User Gallery


Folding@home Statistics
Registered: 05/12/07
Posts: 69,360
Loc: The Inexpressible... Flag
Last seen: 1 hour, 14 minutes
Trusted Cultivator
Re: Mad Season's sterile techniques: Inoculation [Re: Mad Season] * 1
    #22313365 - 09/30/15 10:51 AM (8 years, 3 months ago)

Nice post man!

Love all the extra photos. Really makes it extra clear on what you're doing.

Also, why do you wet the inside of the SAB? Does it help keep it more bacteria-free?


--------------------
"What you must understand is that your physical dimension affects everyone in the higher dimensions as well. All things are interconnected. All things are One. Therefore, if one dimension is broken or out of balance, then all other dimensions will experience repercussions." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin

PROJECT BLUE BOOK ANALYSIS! (312 pages!) | Psychedelics & UFOs | Ready to Contact UFOs? | The Source on Mushrooms:shroomeryhead:| Trippy Gematrix | Dj TeknoLogical | Fentanyl Test Kits R.I.P. Big Worm :tombstone: || The Start of the Ascension Process was 2020. Welcome to the Next Great Era of Earth 🌎🌍🌏                                                         
:sunny::bliss::mushroom2: Oregon Eclipse Festival 2017 :: Aug 19th - 21st :: Pure Paradise :mushroom2::bliss::sunny: :rainbowdrink: Very Effective LSA Extraction Tek :rainbowdrink: | πŸ’§ Advanced Cold Water LSA Extraction Method πŸ’§ | :cacti::bongload: Mescajuana - Mescaline with Marijuana | DMT Dab Bongs | UFO Technology! :shpongle:Shpongle:shpongle:   


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Mad Season's sterile techniques: Inoculation [Re: LogicaL Chaos] * 1
    #22313448 - 09/30/15 11:14 AM (8 years, 3 months ago)

Yes. As air moves around in it the contams and other particles will get stuck to the walls :smile: it aids in keeping the box free of particles. Even moving big objects like jars can disturb the air enough for things to start spreading though. That's why I recommend the biggest clear tote you can find. More air to move means it takes more to move it


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
Offlinebennylava
Bad example


Registered: 05/29/15
Posts: 587
Last seen: 6 years, 9 months
Re: Mad Season's sterile techniques: Inoculation [Re: blindingleaf]
    #22313458 - 09/30/15 11:16 AM (8 years, 3 months ago)

Quote:

Mad Season said:
The pvc collars are so bleh to me. A still air box is much better than a glove box. Glove boxes are so 2003. You'll have a better success rate in a proper sab over a gb.

I mean make it with collars if you want. Just saying that it's not necessary.





So I'm fine just using a bleach solution on my bare bathroom, and then noc'ing my stuff inside a SAB? Doing that, I should be largely free of contamination?

Quote:

blindingleaf said:
inviable spores (usually old) or media is not soft/watery enough.
if u see germination, then it dies back, do not use.




What about bacteria (or something) beating your spores to the punch? What if your spores never germinate, but you never really physically see the contaminants? Is that something that could happen? Or do you always see the mold or bacteria buildup? Can it just be a bad smell?


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Mad Season's sterile techniques: Inoculation [Re: bennylava]
    #22313484 - 09/30/15 11:19 AM (8 years, 3 months ago)

It's possible for nothing, including contams to not be able to germinate for months. It happened with my tampanensis spores... :shrug: not sure why.

If the whole plate gets covered in bacteria it's pretty much garbage, but in the op, the 3rd and 2nd plate, both those plates had bacteria and other shit germinate before mycelium. I was still able to save it. Hope that helps.

As for the bathroom, if the room has fairly still air, and you're actually ok with breathing in bleach.... go for it. Personally I just use soap water lol.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
Offlinebennylava
Bad example


Registered: 05/29/15
Posts: 587
Last seen: 6 years, 9 months
Re: Mad Season's sterile techniques: Inoculation [Re: Mad Season]
    #22313507 - 09/30/15 11:25 AM (8 years, 3 months ago)

Quote:

Mad Season said:
It's possible for nothing, including contams to not be able to germinate for months. It happened with my tampanensis spores... :shrug: not sure why.

If the whole plate gets covered in bacteria it's pretty much garbage, but in the op, the 3rd and 2nd plate, both those plates had bacteria and other shit germinate before mycelium. I was still able to save it. Hope that helps.

As for the bathroom, if the room has fairly still air, and you're actually ok with breathing in bleach.... go for it. Personally I just use soap water lol.




Yeah I'll probably just go the soap and water route, now that you mention it.

But your reply raises another question for me. Inside the SAB, there has to be a very strong alcohol smell. Often times, alcohol is being used inside the box. Just from using it inside my SAB and nowhere else outside of the SAB, it made the whole bathroom smell pretty badly of alcohol fumes. So I can only imagine, what the air is like, inside my petri dishes, and anywhere else that the air gets a chance to get into. Could this potentially be bad for germination, or otherwise hinder mycelium growth?


Edited by bennylava (09/30/15 11:28 AM)


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Mad Season's sterile techniques: Inoculation [Re: bennylava]
    #22313517 - 09/30/15 11:27 AM (8 years, 3 months ago)

It'll be fine :smile: don't need to worry too much about fumes for them. I was just saying for your convenience lol


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3 | 4 | 5 | Next >  [ show all ]

Shop: Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* 1st timer- sterilization techniques? Alice_In_Chains 2,250 6 06/22/02 08:41 AM
by whackedout
* Improvements on this Grow Guide ? badtz 1,410 10 01/23/03 05:32 AM
by Anno
* Dishwasher Sterilization Technique djcontra 4,557 5 01/19/03 04:19 AM
by Krayzie
* sterilization technique maximus_sedaius 1,655 11 11/19/02 04:43 PM
by On_the_Down-Low
* Sterilization Technique? jim_dewit 1,688 8 06/30/02 09:57 PM
by scotsman1
* sterilization technique??? TripleB 913 5 04/14/04 07:12 PM
by ATWAR
* Sterilization Techniques? TrippingBillies 1,226 4 04/29/02 08:43 PM
by Baby_Hitler
* alternative sterilization techniques nuGGs 1,200 3 09/22/02 11:49 PM
by mickey_rourke

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
29,622 topic views. 39 members, 216 guests and 49 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.039 seconds spending 0.012 seconds on 15 queries.