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Shop: North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Mushroom-Hut Liquid Cultures

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OfflineDeadPhan
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how long do unninoculated sterilized jars take to contam in your experience?
    #21888394 - 07/02/15 03:14 PM (8 years, 6 months ago)

I had like 20 sterilized jars that I couldnt get around to inoculate for just shy of 2 weeks. When I was ready I noticed they all took on what seemed to be cobweb mold and had to toss them. I thought ive heard they last longer however ive.never waited this long before myself.


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InvisiblebodhisattaMDiscordReddit
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Re: how long do unninoculated sterilized jars take to contam in your experience? [Re: DeadPhan]
    #21888602 - 07/02/15 03:34 PM (8 years, 6 months ago)

It should be very few that contaminate and if they do with bacteria if they contaminate with mold you didn't sterilize them.


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Offlinethebitterbuffalo26
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Re: how long do unninoculated sterilized jars take to contam in your experience? [Re: DeadPhan]
    #21888615 - 07/02/15 03:36 PM (8 years, 6 months ago)

Hmm  brf? I would kind of think they would just dry out before contaminating... unless they were moved? I'm pretty sure I had a wbs jar that I didn't use for two weeks... no noticeable contamination


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InvisibleSteveRogers
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Re: how long do unninoculated sterilized jars take to contam in your experience? [Re: bodhisatta]
    #21888671 - 07/02/15 03:49 PM (8 years, 6 months ago)

Quote:

bodhisatta said:
It should be very few that contaminate and if they do with bacteria if they contaminate with mold you didn't sterilize them.




Agreed.


--------------------
"General, I am loyal to nothing......except The Dream"


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InvisibleJuiceh
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Re: how long do unninoculated sterilized jars take to contam in your experience? [Re: SteveRogers]
    #21888719 - 07/02/15 03:58 PM (8 years, 6 months ago)

I have jars that were sterilized months ago. No contams.


--------------------
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OfflineZeroBoyWD
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Re: how long do unninoculated sterilized jars take to contam in your experience? [Re: Juiceh]
    #21888889 - 07/02/15 04:23 PM (8 years, 6 months ago)

I typically leave one uninoculated as a control. If it gets contaminated then I know I fucked up sterilization. If other jars get contams but my control does not then I know I screwed up during inoculation. Science!


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I'm done trying to be "right." I want the exchange of ideas to be the currency of my economy. If you have something I can use, great. I'll try it out. If you think my methods are shit, great. Don't use it. We all want yields.



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OfflineTravelAgency
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Re: how long do unninoculated sterilized jars take to contam in your experience? [Re: ZeroBoyWD]
    #21888970 - 07/02/15 04:36 PM (8 years, 6 months ago)

Quote:

ZeroBoyWD said:
I typically leave one uninoculated as a control. If it gets contaminated then I know I fucked up sterilization. If other jars get contams but my control does not then I know I screwed up during inoculation. Science!




That's really smart- thanks for the tip!


--------------------





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OfflineDeadPhan
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Re: how long do unninoculated sterilized jars take to contam in your experience? [Re: TravelAgency]
    #21889196 - 07/02/15 05:43 PM (8 years, 6 months ago)

All grain.


--------------------
 


Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


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OfflinePinPornProducer
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Re: how long do unninoculated sterilized jars take to contam in your experience? [Re: DeadPhan]
    #21889234 - 07/02/15 05:55 PM (8 years, 6 months ago)

I wouldn't leave em too long, they will loose moisture


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Offlineblackout
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Re: how long do unninoculated sterilized jars take to contam in your experience? [Re: PinPornProducer]
    #21889253 - 07/02/15 06:00 PM (8 years, 6 months ago)

Quote:

PinPornProducer said:
I wouldn't leave em too long, they will loose moisture


You can cover the filters, I used to use jars with no filters. I have jars with no contams and I have no idea how old many are, some could be 10 years old, definitely lots 5+ years old. I found another few just the other day. I used to keep them to test out if my more unusual sterilization attempts would work. I stupidly did not date most of them, or keep notes.


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OfflinePinPornProducer
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Re: how long do unninoculated sterilized jars take to contam in your experience? [Re: blackout]
    #21889288 - 07/02/15 06:08 PM (8 years, 6 months ago)

I suppose if you cover the filter they would stay longer but I noticed slower colonization time from letting jars sit sterilized for 10 days before g2ging.

5-10 years, damn. You don't plan on using them do you lol?


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InvisiblebodhisattaMDiscordReddit
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Re: how long do unninoculated sterilized jars take to contam in your experience? [Re: blackout]
    #21889308 - 07/02/15 06:12 PM (8 years, 6 months ago)

Our grain sterilization is based on a SAL(sterility assurance level) of 6 log reduction 10E-6 but we also have th assumption that there's 10E6 CFU before sterilization so it takes 6 logs of kill to get to theoretical 0. Sanitation is 3-4 logs 99.9 99.99 kill but not good enough. After 6 logs to theoretical zero the industry considers sterilization another 6 logs after the CFUs are killed to get a 10E-6 jar that means there's a 1:1000000 chance of one CFU per jar. If sterilization is adequate. If you sterilize say to 10E-2 there exist a possibility that 1:100 jars may have 1CFU. If you sterilize a jar theoretically it shouldn't contam. But grains are tough to sterile since there not liquid media.


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OfflineZeroBoyWD
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Re: how long do unninoculated sterilized jars take to contam in your experience? [Re: bodhisatta]
    #21889327 - 07/02/15 06:15 PM (8 years, 6 months ago)

Quote:

bodhisatta said:
Our grain sterilization is based on a SAL(sterility assurance level) of 6 log reduction 10E-6 but we also have th assumption that there's 10E6 CFU before sterilization so it takes 6 logs of kill to get to theoretical 0. Sanitation is 3-4 logs 99.9 99.99 kill but not good enough. After 6 logs to theoretical zero the industry considers sterilization another 6 logs after the CFUs are killed to get a 10E-6 jar that means there's a 1:1000000 chance of one CFU per jar. If sterilization is adequate. If you sterilize say to 10E-2 there exist a possibility that 1:100 jars may have 1CFU. If you sterilize a jar theoretically it shouldn't contam. But grains are tough to sterile since there not liquid media.



.....Yeah we rebuilt a 454 big block one time, it was pretty cool.


--------------------
I'm done trying to be "right." I want the exchange of ideas to be the currency of my economy. If you have something I can use, great. I'll try it out. If you think my methods are shit, great. Don't use it. We all want yields.



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InvisiblebodhisattaMDiscordReddit
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Re: how long do unninoculated sterilized jars take to contam in your experience? [Re: ZeroBoyWD]
    #21889539 - 07/02/15 07:01 PM (8 years, 6 months ago)

I make 3000 gal of beer a day its pretty cool


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OfflineZeroBoyWD
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Re: how long do unninoculated sterilized jars take to contam in your experience? [Re: bodhisatta]
    #21889556 - 07/02/15 07:04 PM (8 years, 6 months ago)

We should party


--------------------
I'm done trying to be "right." I want the exchange of ideas to be the currency of my economy. If you have something I can use, great. I'll try it out. If you think my methods are shit, great. Don't use it. We all want yields.



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OfflineNcogneato
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Re: how long do unninoculated sterilized jars take to contam in your experience? [Re: bodhisatta]
    #21889877 - 07/02/15 08:23 PM (8 years, 6 months ago)

Quote:

bodhisatta said:
I make 3000 gal of beer a day its pretty cool



Hey, I need a job. I'm a pretty smart feller'!

OP,
It sounds like it was a good thing that you didn't inoculate. Now you know to re examine your sterilization techniques. There's always a silver lining.


--------------------
Psilocybin.........the poor man's Cancun.
                   


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Invisibleelasticaltiger
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Re: how long do unninoculated sterilized jars take to contam in your experience? [Re: Ncogneato]
    #21889883 - 07/02/15 08:25 PM (8 years, 6 months ago)

I have 1 year old WBS and 2 year old PF Tek jars that I never inoculated without any 'visible' signs of contamination.

Bacteria doesn't always 'show' though.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

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Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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Invisibleelasticaltiger
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Re: how long do unninoculated sterilized jars take to contam in your experience? [Re: elasticaltiger]
    #21889933 - 07/02/15 08:35 PM (8 years, 6 months ago)

Oh and my above comment is not an endorsement for prepping a butt load of jars of head of time.

I have inoculated jars over a month after they were PC'd with decent results.  I can't say I noticed a difference from the ones I inoculated the day after sterilization but the results weren't any better either.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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Invisibleazur
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Re: how long do unninoculated sterilized jars take to contam in your experience? [Re: DeadPhan]
    #21889990 - 07/02/15 08:47 PM (8 years, 6 months ago)

Idk why we're always seeing this question. Is it not obvious that the best time to :doggystyle: a jar is as soon as it cools from the pc cycle?


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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OfflineZeroBoyWD
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Re: how long do unninoculated sterilized jars take to contam in your experience? [Re: azur]
    #21890002 - 07/02/15 08:49 PM (8 years, 6 months ago)

I think some people sterilize 12 then find it difficult to stretch their syringe to more than 10. I use three holes instead of four, works fine


--------------------
I'm done trying to be "right." I want the exchange of ideas to be the currency of my economy. If you have something I can use, great. I'll try it out. If you think my methods are shit, great. Don't use it. We all want yields.



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Invisibleazur
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Re: how long do unninoculated sterilized jars take to contam in your experience? [Re: ZeroBoyWD]
    #21890006 - 07/02/15 08:50 PM (8 years, 6 months ago)

failure to plan is planning to fail


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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OfflineZeroBoyWD
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Re: how long do unninoculated sterilized jars take to contam in your experience? [Re: azur]
    #21890039 - 07/02/15 08:55 PM (8 years, 6 months ago)

Well you can plan to shoot up 12, but it takes a steady touch to only give 1/4cc per port.

10cc / .25cc = 40 shots
40 / 4 holes = 10 jars
40 / 3 holes = 13 jars

Gives you a little wiggle room

[Edit to fix faulty retard math]


--------------------
I'm done trying to be "right." I want the exchange of ideas to be the currency of my economy. If you have something I can use, great. I'll try it out. If you think my methods are shit, great. Don't use it. We all want yields.



Edited by ZeroBoyWD (07/02/15 08:56 PM)


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Invisibleelasticaltiger
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Re: how long do unninoculated sterilized jars take to contam in your experience? [Re: azur]
    #21890193 - 07/02/15 09:32 PM (8 years, 6 months ago)

Quote:

azur said:
failure to plan is planning to fail




And practice makes permanent. Only perfect practice makes perfect.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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OfflineZeroBoyWD
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Re: how long do unninoculated sterilized jars take to contam in your experience? [Re: elasticaltiger]
    #21890419 - 07/02/15 10:25 PM (8 years, 6 months ago)

A penny saved is worth two in the bush

Anyone else want to spout any more generic cliches that don't give any real advice =P

Quote:

azur said:
failure to plan is planning to fail



Its only failure when you don't learn anything

Quote:

elasticaltiger said:
Quote:

azur said:
failure to plan is planning to fail




And practice makes permanent. Only perfect practice makes perfect.



Nah, 10,000 hours is what you need to reach perfection.


--------------------
I'm done trying to be "right." I want the exchange of ideas to be the currency of my economy. If you have something I can use, great. I'll try it out. If you think my methods are shit, great. Don't use it. We all want yields.



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Invisibleazur
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Re: how long do unninoculated sterilized jars take to contam in your experience? [Re: ZeroBoyWD]
    #21890435 - 07/02/15 10:29 PM (8 years, 6 months ago)

Yea. How about chevy's suck. That's a poor man's car. You can build a 350 way cheaper than you can build a 302. And i won't even to start talking about a porsche engine.

The point was if you have jars leftover, figure out a way to use them. And if you can't, then FOTTSE


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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OfflineZeroBoyWD
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Re: how long do unninoculated sterilized jars take to contam in your experience? [Re: azur]
    #21890929 - 07/03/15 12:30 AM (8 years, 6 months ago)

Found On Road Dead. Ford is a one trick pony, if you'll pardon the pun =D

I'm pretty convinced Ford's engineers design their car's to fall right apart after 100k. But nah, I never really rebuilt one, I just wanted to say something technical after bodhie's jargon. I'd LOVE to restore a 69 Impala though...

But! Back on topic, I agree, have a little foresight before you set to work... But in my current state thats the pot calling the kettle a whore.


--------------------
I'm done trying to be "right." I want the exchange of ideas to be the currency of my economy. If you have something I can use, great. I'll try it out. If you think my methods are shit, great. Don't use it. We all want yields.



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InvisibleJuiceh
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Re: how long do unninoculated sterilized jars take to contam in your experience? [Re: DeadPhan]
    #21896244 - 07/04/15 10:53 AM (8 years, 6 months ago)

Quote:

DeadPhan said:
I had like 20 sterilized jars that I couldnt get around to inoculate for just shy of 2 weeks. When I was ready I noticed they all took on what seemed to be cobweb mold and had to toss them. I thought ive heard they last longer however ive.never waited this long before myself.



I think we all missed a possibly important question here... What kind of filter do you have on your jar lids?


--------------------
Trade List

How I make 15 lbs of casing in a 941. How I line 12 shoeboxes with 1 39gal bag. How I Deep Fry Cubes!
RogerRabbit said:You need a bigger pressure cooker for this hobby. RR


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