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OfflineGreen Bastard
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Agar plate help
    #21887840 - 07/02/15 01:00 PM (8 years, 6 months ago)

Hey there folks.
I've been transferring from 8 different cubensis fruit clones. I'm 4, or 5 transfers, depending on the plate, away from the first tissue to agar. I don't really feel the need to end up with pure isolates. Just some reliable clones to put to slants, and then keep perpetuating.

Here's 3 examples that I'm curious about. The dramatic differences in growth are either troubling, or promising. But, I feel like I'm kinda shooting in the dark here. Any insight, into these, would be greatly appreciated.







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Invisibled0urd3n
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Registered: 09/15/10
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Re: Agar plate help [Re: Green Bastard]
    #21887853 - 07/02/15 01:04 PM (8 years, 6 months ago)

I have found this thread very helpful as far as deciding on which sectors to take.


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InvisibleMunchauzen
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Re: Agar plate help [Re: Green Bastard]
    #21887858 - 07/02/15 01:06 PM (8 years, 6 months ago)

at 5 transfers I'd say you are just starting to see sectoring of individual strains. I might transfer some of those aggressive ones in the top two because of how uneven they are, but the bottom one is def good to go and still has multiple visible strains that are growing at a somewhat consistent rate.


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OfflineGreen Bastard
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Re: Agar plate help [Re: Munchauzen]
    #21887994 - 07/02/15 01:43 PM (8 years, 6 months ago)

Cool. Thank you for the quick replies! So, no worries that those weaker sections could indicate any underlying contaminates? My older plates have all remained, to my eye, contam free.

Any issues with just taking these to slants, and rye jars, and stopping selective transfers?


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InvisibleMunchauzen
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Re: Agar plate help [Re: Green Bastard]
    #21888031 - 07/02/15 01:50 PM (8 years, 6 months ago)

if there were contaminates, you would see a barrier of resistance where the two species meet and do not intermingle.

nothing can hide on agar.


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Invisible36fuckin5
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Re: Agar plate help [Re: Munchauzen]
    #21888058 - 07/02/15 01:56 PM (8 years, 6 months ago)

You could slant it up now, but why not just isolate them? You're already ridiculously close.


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i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineGreen Bastard
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Re: Agar plate help [Re: 36fuckin5]
    #21888116 - 07/02/15 02:11 PM (8 years, 6 months ago)

I'm feeling much reassured, that my time infront of the flow hood has been time well spent!

I've been so happy with my MS monos, as of late, that i imagine that any clone grow is gonna blow me away. I have a bunch of jars that are currently at 90%, from some first and second transfer plates, that I can't wait to spawn to bulk.

My concern, regarding chasing a true isolate, are the issues I've read about as far as the strong possibility of sub par isolates. The supposed reliability of non isolate clones, is my main goal.

That said, I have plenty of plates, and slant tubes, at my disposal. I suppose I can take some more to grain masters, and slants, now, while continuing to isolate the more promising cultures...

Getting a bit overwhelmed by the number of cultures that I have piling up on plates, and by my ever expanding nomenclature... Fun stuff though, especially with the great resources here, to keep me on track.

Thank you all, for the help so far!!


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InvisibleMunchauzen
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Re: Agar plate help [Re: Green Bastard]
    #21888166 - 07/02/15 02:22 PM (8 years, 6 months ago)

yeah agree with 36f5 go for the isolates on the obvious strains before you slant them. its very close. just grow the isolated cultures out to test them while keeping other ms grows going. a potent isolate is pretty much the most reliable thing you can get. I honestly wouldn't rely on clones. I've personally had *legendary* clones perform subpar for me using standard techniques.


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