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InvisibleTFI
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Any way to tell if wbs jar is contamed at 100% colonization?
    #21887074 - 07/02/15 09:32 AM (8 years, 6 months ago)

Ok so im messing with agar atm but have three wbs jars that just finished colonizing.

Last grow went bad due to bad spawn, had little white specks in jars last time i believe thats yeast infection but not totally sure..

Is their anyway i can check these jars other than opening them or looking for contams visibly because i see alot of rhizmorphic growth but im curious if theirs another way other than g2g them an hope for the best.. They lookn fine but since the last grow looked ok im nervous


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OfflineCharleskun
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Re: Any way to tell if wbs jar is contamed at 100% colonization? [Re: TFI]
    #21887085 - 07/02/15 09:34 AM (8 years, 6 months ago)

Pictures would help.


--------------------
All I see Is darkness


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InvisibleTFI
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Re: Any way to tell if wbs jar is contamed at 100% colonization? [Re: Charleskun]
    #21887188 - 07/02/15 09:52 AM (8 years, 6 months ago)

camera is broken since i dropped it.

Its a classic 100% colonized wbs jar completely white with little rope style growth forming (the good kind) no visible sign of contam at all.


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OfflineThunderbird310
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Re: Any way to tell if wbs jar is contamed at 100% colonization? [Re: TFI]
    #21887238 - 07/02/15 10:03 AM (8 years, 6 months ago)

The only way to check for contams without opening is by looking at them. Once fully colonized the spawn is fairly contaminate resistant. Are you mixing with a properly pasturized bulk substrate? Without pics you will have to use your best judgment. Look at the contamination link under general cultivation. It has photos of the most common contaminants.


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OfflinePsilosoulful

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Re: Any way to tell if wbs jar is contamed at 100% colonization? [Re: TFI]
    #21887243 - 07/02/15 10:05 AM (8 years, 6 months ago)

Quote:

TFI said:
camera is broken since i dropped it.

Its a classic 100% colonized wbs jar completely white with little rope style growth forming (the good kind) no visible sign of contam at all.



Before spawning it, crack the lid and give it a good smell. If it has a slightly sweet smell, then there is probably bacterial contamination of some sort. If it has that fresh mushroom smell, then it is most likely healthy spawn and would colonize well. :smile:


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Offlinecaveman421
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Re: Any way to tell if wbs jar is contamed at 100% colonization? [Re: TFI]
    #21887245 - 07/02/15 10:05 AM (8 years, 6 months ago)

Last i checked at 100% colonization theres nothing to contaminate....


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InvisibleTFI
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Re: Any way to tell if wbs jar is contamed at 100% colonization? [Re: caveman421]
    #21887441 - 07/02/15 10:58 AM (8 years, 6 months ago)

Right on ok. I plan to g2g this jar to others for spawn. I herd somewhere if you break it up an let it recolonize that is one way to see if its ok. smell is always the number one way to i do believe.

Nothing visible so i guess here goes nothing lol


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OfflinePsilosoulful

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Re: Any way to tell if wbs jar is contamed at 100% colonization? [Re: caveman421]
    #21887448 - 07/02/15 11:01 AM (8 years, 6 months ago)

Quote:

caveman421 said:
Last i checked at 100% colonization theres nothing to contaminate....



Most of time, yes, but there's always a possibility. That's why the smell test is a surefire way to know for sure.


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Offlinecaveman421
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Re: Any way to tell if wbs jar is contamed at 100% colonization? [Re: Psilosoulful]
    #21890790 - 07/02/15 11:49 PM (8 years, 6 months ago)

Word.


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InvisibleMad Season
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Re: Any way to tell if wbs jar is contamed at 100% colonization? [Re: caveman421]
    #21890806 - 07/02/15 11:54 PM (8 years, 6 months ago)

Oh dear lord no. I'll get a quote from when I had this debate awhile back. There's a big difference between fully colonized and cleanly colonized.
Quote:

Mad Season said:
Well there's a difference between colonized and cleanly colonized.

This is colonized:

Lots of members have noticed cobweb and trich from jars that look like this or better. Wet spots are bacteria. Not wet grains or wet spots. To colonize cleanly is the hardest. I had to change my pc times to 2 hours to reduce it.

This is (ALMOST) cleanly colonized, that's how hard it is to have it completely clean. This isn't even completely clean:


This is completely clean spawn:



Anyone who's done this for awhile will testify this is the hardest part of the hobby and is what will dictate the success or failure of bulk.



All those jars had a mushroomy smell. A miniscule amount of bacteria will get overpowered by the mushroom musk. Especially if it is covering the surface


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contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (07/02/15 11:55 PM)


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OfflinePsilosoulful

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Re: Any way to tell if wbs jar is contamed at 100% colonization? [Re: Mad Season]
    #21890853 - 07/03/15 12:07 AM (8 years, 6 months ago)

Quote:

Mad Season said:
Oh dear lord no. I'll get a quote from when I had this debate awhile back. There's a big difference between fully colonized and cleanly colonized.
Quote:

Mad Season said:
Well there's a difference between colonized and cleanly colonized.

This is colonized:

Lots of members have noticed cobweb and trich from jars that look like this or better. Wet spots are bacteria. Not wet grains or wet spots. To colonize cleanly is the hardest. I had to change my pc times to 2 hours to reduce it.

This is (ALMOST) cleanly colonized, that's how hard it is to have it completely clean. This isn't even completely clean:


This is completely clean spawn:



Anyone who's done this for awhile will testify this is the hardest part of the hobby and is what will dictate the success or failure of bulk.



All those jars had a mushroomy smell. A miniscule amount of bacteria will get overpowered by the mushroom musk. Especially if it is covering the surface



Wow I never knew grain could contaminate that easily, my understanding was that 100% fully colonized mycelium could overtake any hidden contaminates once mixed with properly pasteurized substrates, at least most of the time.

I've had jars that looked to be bacterial because of wet grains sticking to the side of the jar, but they colonized fine when spawned to bulk. I guess it really is hit or miss. :shrug:


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InvisibleMad Season
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Re: Any way to tell if wbs jar is contamed at 100% colonization? [Re: Psilosoulful]
    #21890892 - 07/03/15 12:19 AM (8 years, 6 months ago)

They didn't have wet grains stuck to the jar side. It was bacteria. A properly pasteurized bulk substrate has beneficial microbes that fight bacteria off. In fact if mycelium takes the upper hand like the jars in those pics, more often than not it won't be a problem. However it will ruin pinsets, slow down colonization rates, and EXTREMELY increase chances of other contaminations like trich or cobweb.

All those jars were used to make their own separate trays. The ones that were clean easily pumped out twice as much weight.

Dirty spawn:


Clean spawn:


Edited by Mad Season (07/07/16 04:08 AM)


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OfflinePsilosoulful

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Re: Any way to tell if wbs jar is contamed at 100% colonization? [Re: Mad Season]
    #21891726 - 07/03/15 08:28 AM (8 years, 6 months ago)

That's good to know, I'll definitely pay more attention to my PC'ing times/inoculation procedures, I'd hate to prep all those grain jars and have them turn out to be bad spawn in the end.


Edited by Psilosoulful (07/03/15 08:34 AM)


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InvisibleTFI
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Re: Any way to tell if wbs jar is contamed at 100% colonization? [Re: Psilosoulful]
    #21897180 - 07/04/15 03:30 PM (8 years, 6 months ago)

Mad season thats badass! what happen to me i spawned a shot load thinking its ok wasnt to bad, and like the first tray all my tubs pinned no fruits.. Thats a great representation. Thanks


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Offlinespacechildo
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Re: Any way to tell if wbs jar is contamed at 100% colonization? [Re: TFI]
    #21897358 - 07/04/15 04:12 PM (8 years, 6 months ago)

yeah thats a great post mad!

there's a lot of threads where people use unhealthy spawn and claim success with poor sterile tech
where you can see the BE is hurting from the cube myc having to fight another organism.

Imma take note and PM you next time I see it so you can post the pics! :tongue2:


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InvisibleMad Season
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Re: Any way to tell if wbs jar is contamed at 100% colonization? [Re: spacechildo]
    #21897459 - 07/04/15 04:40 PM (8 years, 6 months ago)

:lol::rockon: you got permission to use em space. However if you're too lazy to find them just pm me haha


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Offlinespacechildo
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Re: Any way to tell if wbs jar is contamed at 100% colonization? [Re: Mad Season]
    #21897475 - 07/04/15 04:44 PM (8 years, 6 months ago)

:fuckinawesome:

Now watch this drive! :stars:


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OfflineIcon
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Re: Any way to tell if wbs jar is contamed at 100% colonization? [Re: spacechildo]
    #21897835 - 07/04/15 05:48 PM (8 years, 6 months ago)

What about shaking a 100% colonized jar and letting it re-colonize. Would that be any proof of clean / suspicious spawn? Like would a hidden trich node start to flourish if broken up and shaken around a jar? Might be worth waiting a few extra days if I can trick jars into showing themselves.


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InvisibleTFI
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Re: Any way to tell if wbs jar is contamed at 100% colonization? [Re: Icon]
    #21904385 - 07/06/15 07:48 AM (8 years, 6 months ago)

Ok thats twice i have herd that so im gonna make a personal note an say thats a great idea lol


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Invisibleinsanemike

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Re: Any way to tell if wbs jar is contamed at 100% colonization? [Re: Icon]
    #21904560 - 07/06/15 08:47 AM (8 years, 6 months ago)

Quote:

Icon said:
What about shaking a 100% colonized jar and letting it re-colonize. Would that be any proof of clean / suspicious spawn? Like would a hidden trich node start to flourish if broken up and shaken around a jar? Might be worth waiting a few extra days if I can trick jars into showing themselves.




Yes. After shaking a fully colonized grain jar (highly suggest this practice everytime), it should fully recover in 3 days. If it takes any longer without a visual contamination, it's probably infected with bacteria. A majority of the time, mold will show up a day or two after the shake.


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