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OfflineErik77johansson77
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Registered: 03/12/15
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Only tomentose in agar?
    #21886142 - 07/02/15 02:38 AM (8 years, 6 months ago)

Hi guys,

I made a simple agar with potato flakes, dextrose, agar. Injected with MS.

I made some agar transfers now, I'm into the fourth one now.
I understood that less nutrient = the mycelium will look for food, correct?

My question is: why cant I see rhiz. growth, what is the reason?

I thought I had to make few transfers to make isolation, which I did. I even did the transfer before they grew a lot.


Here is one I kept, when I transferred to my current. I let it grow few more days, hoped to see some rhiz. but nothing!

Should I start over?


--------------------
/Erik


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Offlinespacechildo
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Registered: 01/24/13
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Re: Only tomentose in agar? [Re: Erik77johansson77]
    #21886213 - 07/02/15 03:35 AM (8 years, 6 months ago)

no need to start over, rhizo or tomentose we dont discriminate here :super:


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OfflineErik77johansson77
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Re: Only tomentose in agar? [Re: spacechildo]
    #21886250 - 07/02/15 04:01 AM (8 years, 6 months ago)

heheh nice one... I just thought rhizo is the better mycelium.

I've only got tomentose in my jars (2 liters), and they are taking forever. Been inoculated them with MS 24/4 and it's 2/7 now Like 98% done, and only cottony mycelium :/


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/Erik


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Offlinespacechildo
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Registered: 01/24/13
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Re: Only tomentose in agar? [Re: Erik77johansson77]
    #21886266 - 07/02/15 04:10 AM (8 years, 6 months ago)

probably mold or bacteria or both. jars dont take 2,5 months to finish.
2 liter jars could go anaerobic in the center too. have you ever shook the jars?


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