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Mushie23
Entheogens



Registered: 11/19/10
Posts: 789
Loc: PNW
Last seen: 7 years, 10 months
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Injecton ports, trusted/experienced help please
#21878918 - 06/30/15 04:25 PM (8 years, 6 months ago) |
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Followed injection port construction method by RR to the T. However, would really appreciate advice/tips on the following.
When using BRF: Injecting to all 4 corners is now a challenge with the injection port, as the syringe won't reach the opposite side of the of the jar through the port.(Also, the syringes are shorter than usual) Should injection ports even be used for BRF method? Or just stick to injecting straight through micospore tape?(this simple method has always seemed to work).
When using RYE: Would love advice on injecting Rye. Great success last time, but current syringes are much shorter than last time and won't reach the rye...especially if leaving some space for shaking later. Would it be safe to take the lid off the jar in front of flow hood and inject that way?
hopefully that made sense? Injecting through tape before was working fine, but want to move onto a cleaner, more productive method.
Thank you shroomerites!
-------------------- Eat Mushrooms----plug into life and nature.
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
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Re: Injecton ports, trusted/experienced help please [Re: Mushie23]
#21879059 - 06/30/15 04:53 PM (8 years, 6 months ago) |
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Quote:
Mushie23 said: Should injection ports even be used for BRF method? Or just stick to injecting straight through micospore tape?(this simple method has always seemed to work).
1 no, 2 yes.
Quote:
Mushie23 said: When using RYE: Would love advice on injecting Rye. Great success last time, but current syringes are much shorter than last time and won't reach the rye...especially if leaving some space for shaking later. Would it be safe to take the lid off the jar in front of flow hood and inject that way?
you dont want the needle touching the rye. just squirt a few drops against the glass in a 360 motion. taking the lid off in a SAB or in front of a FH is the way most people do it, except we use agar wedges and not spore syringes.
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Mushie23
Entheogens



Registered: 11/19/10
Posts: 789
Loc: PNW
Last seen: 7 years, 10 months
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Re: Injecton ports, trusted/experienced help please [Re: spacechildo]
#21879259 - 06/30/15 05:35 PM (8 years, 6 months ago) |
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Thank you for the clarification, very appreciated spacechildo!
-------------------- Eat Mushrooms----plug into life and nature.
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OPB
Wanderer

Registered: 05/30/15
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Re: Injecton ports, trusted/experienced help please [Re: Mushie23]
#21879616 - 06/30/15 07:02 PM (8 years, 6 months ago) |
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With brf you don't need them or micropore tape. Just drill 3-4 holes near the edge of the lid. The dry layer of vermiculite on top is what protects the contents from contamination. If you really want to use a self healing port just make 1 or 2 but be sure to place them near the edge so you can run the solution down the wall of the jar.
For rye, again, have your port near an edge. Just tip the jar and angle the syringe so that you can run the solution down the glass. It's fine if the needle doesn't reach the rye...that is assuming you are doing this on earth with gravity and such
-------------------- "Wasn't that fun"
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mushpunx
Fungus Punk



Registered: 04/20/14
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Re: Injecton ports, trusted/experienced help please [Re: OPB]
#21880059 - 06/30/15 08:31 PM (8 years, 6 months ago) |
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If you are going to be working with grains you are going to want to learn to open the lids properly in a still air box.
Injecting spores into grain is risky business anyways, BRF is kinda forgiving but grain certainly arent!
All grows on grain should start with agar. But untill you start working with it you can simply open the lid in your still air box, squirt in a drop of spores and close it back up.
Easy!
But yea youre way more likely to get a contam from the syringe than from opening the lid if you use proper sterile technique, so it renders the injection port a little useless really
--------------------
 Amateur Mycologists United AMU Q&A
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OPB
Wanderer

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Re: Injecton ports, trusted/experienced help please [Re: mushpunx]
#21881158 - 07/01/15 01:10 AM (8 years, 6 months ago) |
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Quote:
mushpunx said: If you are going to be working with grains you are going to want to learn to open the lids properly in a still air box.
Injecting spores into grain is risky business anyways, BRF is kinda forgiving but grain certainly arent!
All grows on grain should start with agar. But untill you start working with it you can simply open the lid in your still air box, squirt in a drop of spores and close it back up.
Easy!
But yea youre way more likely to get a contam from the syringe than from opening the lid if you use proper sterile technique, so it renders the injection port a little useless really
What about injecting makes contamination more likely? I was under the impression that using sterile technique and flame sterilization that your chances of contamination were very low. I ask out of sheer curiosity as you certainly have more experience and I think it could be helpful for the OP as well.
-------------------- "Wasn't that fun"
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FriedEgg



Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
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Re: Injecton ports, trusted/experienced help please [Re: OPB]
#21881340 - 07/01/15 02:00 AM (8 years, 6 months ago) |
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I think he's referring to the fact that spore syringes are not sterile.
-------------------- (Yes, the egg is real)
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Injecton ports, trusted/experienced help please [Re: FriedEgg]
#21881360 - 07/01/15 02:07 AM (8 years, 6 months ago) |
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Quote:
FriedEgg said: I think he's referring to the fact that spore syringes are not sterile.
Since spores came from caps that fruited in open air. Cakes are pretty lenient and don't contaminate easily, but actually put a syringe under a microscope (they are for microscopy after all) and it has more than just cube spores in it. Bacteria and other stuff is there too.
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FriedEgg



Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
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Re: Injecton ports, trusted/experienced help please [Re: Mad Season]
#21881367 - 07/01/15 02:08 AM (8 years, 6 months ago) |
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Quote:
Mad Season said:
Quote:
FriedEgg said: I think he's referring to the fact that spore syringes are not sterile.
Since spores came from caps that fruited in open air. Cakes are pretty lenient and don't contaminate easily, but actually put a syringe under a microscope (they are for microscopy after all) and it has more than just cube spores in it. Bacteria and other stuff is there too.
exactly, i was just too lazy at the moment to explain it in full
-------------------- (Yes, the egg is real)
How to post pics
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Chk
Reverser


Registered: 09/06/13
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Loc: France
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Re: Injecton ports, trusted/experienced help please [Re: Mushie23]
#21881460 - 07/01/15 02:45 AM (8 years, 6 months ago) |
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Plug you needle in your injection port, put everything at an 45 angle, and rotate while injecting slowly.
Edit: don't inject IN the substrate in that case obviously
And you can't do that if you got a dry verm layer, ofc.
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Edited by Chk (07/01/15 02:50 AM)
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mushpunx
Fungus Punk



Registered: 04/20/14
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Re: Injecton ports, trusted/experienced help please [Re: Mad Season]
#21881740 - 07/01/15 06:06 AM (8 years, 6 months ago) |
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Quote:
Mad Season said:
Quote:
FriedEgg said: I think he's referring to the fact that spore syringes are not sterile.
Since spores came from caps that fruited in open air. Cakes are pretty lenient and don't contaminate easily, but actually put a syringe under a microscope (they are for microscopy after all) and it has more than just cube spores in it. Bacteria and other stuff is there too.
I really had no idea why people would talk about contam problems so much when I first started growing, I have NevER had a cake contaminate! I even got away with plenty of bulk growns made with grain spawn injected with spores. But man once you start getting problems its an endless battle 
It wasnt untill I started working with agar that I saw what people meant when they say it is quite rare for a spore syringe to be truly clean. More frequently then not when I germinate spores on agar I have to isolate away from bacteria, sometimes mold colonies. All stuff in your syringe that you might get away with on a BRF cake but it will certainly not fly on grain.
So what Im saying is you are more likely to get contams into yiur grain jars from the syringe itself than opening the jar to squirt the spores in (under proper conditions in still air box). So the little bit of protection it might offer isnt really worth the experience you will get learning sterile procedure. Its essentially the same motions yiu will need for agar work and all the fun stuff involved in making grain spawn 
To the OP: you CAN have very sucessfull grows by syringe to grain so I hope this 1 turns out fine but look into agar for next grow!
--------------------
 Amateur Mycologists United AMU Q&A
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Dr.Satan
Mad Professor



Registered: 06/26/15
Posts: 2,182
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Re: Injecton ports, trusted/experienced help please [Re: mushpunx]
#21881911 - 07/01/15 07:48 AM (8 years, 6 months ago) |
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Quote:
mushpunx said:
Quote:
Mad Season said:
Quote:
FriedEgg said: I think he's referring to the fact that spore syringes are not sterile.
Since spores came from caps that fruited in open air. Cakes are pretty lenient and don't contaminate easily, but actually put a syringe under a microscope (they are for microscopy after all) and it has more than just cube spores in it. Bacteria and other stuff is there too.
I really had no idea why people would talk about contam problems so much when I first started growing, I have NevER had a cake contaminate! I even got away with plenty of bulk growns made with grain spawn injected with spores. But man once you start getting problems its an endless battle 
It wasnt untill I started working with agar that I saw what people meant when they say it is quite rare for a spore syringe to be truly clean. More frequently then not when I germinate spores on agar I have to isolate away from bacteria, sometimes mold colonies. All stuff in your syringe that you might get away with on a BRF cake but it will certainly not fly on grain.
So what Im saying is you are more likely to get contams into yiur grain jars from the syringe itself than opening the jar to squirt the spores in (under proper conditions in still air box). So the little bit of protection it might offer isnt really worth the experience you will get learning sterile procedure. Its essentially the same motions yiu will need for agar work and all the fun stuff involved in making grain spawn 
To the OP: you CAN have very sucessfull grows by syringe to grain so I hope this 1 turns out fine but look into agar for next grow!
Holy shit cakes very nice pics in sig! My mouth watered a bit :O
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OPB
Wanderer

Registered: 05/30/15
Posts: 198
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Re: Injecton ports, trusted/experienced help please [Re: Dr.Satan]
#21882108 - 07/01/15 09:18 AM (8 years, 6 months ago) |
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Thanks for clearing that up. I hope this was useful for the OP and not just a thread jack...
-------------------- "Wasn't that fun"
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FriedEgg



Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
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Re: Injecton ports, trusted/experienced help please [Re: mushpunx]
#21882324 - 07/01/15 10:24 AM (8 years, 6 months ago) |
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Quote:
mushpunx said: To the OP: you CAN have very sucessfull grows by syringe to grain so I hope this 1 turns out fine but look into agar for next grow!

When I get a syringe, I usually put it on agar first. Then I immediately inoculate some grains with the syringe. If they turn out ok, it's a nice early bonus before my clean agar culture is ready. In other words, I don't count on it working but if it does, woohoo!
-------------------- (Yes, the egg is real)
How to post pics
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Mushie23
Entheogens



Registered: 11/19/10
Posts: 789
Loc: PNW
Last seen: 7 years, 10 months
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Re: Injecton ports, trusted/experienced help please [Re: OPB]
#21882713 - 07/01/15 12:21 PM (8 years, 6 months ago) |
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Quote:
OPB said: With brf you don't need them or micropore tape. Just drill 3-4 holes near the edge of the lid. The dry layer of vermiculite on top is what protects the contents from contamination. If you really want to use a self healing port just make 1 or 2 but be sure to place them near the edge so you can run the solution down the wall of the jar.
For rye, again, have your port near an edge. Just tip the jar and angle the syringe so that you can run the solution down the glass. It's fine if the needle doesn't reach the rye...that is assuming you are doing this on earth with gravity and such 
I don't really wanna use injection ports for BRF, I did this simply to see what would happen...which was nothing:) For this, I'll go back to simply injecting through the drill holes.
Had great success with rye and injecting 3 sides through drilled holes and tape. This was the Mother, which gave birth to 9 more jars in still air box. Will keep in mind to tip the jar more next time with innoc'n.
Thank you Thank you!
-------------------- Eat Mushrooms----plug into life and nature.
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Mushie23
Entheogens



Registered: 11/19/10
Posts: 789
Loc: PNW
Last seen: 7 years, 10 months
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Re: Injecton ports, trusted/experienced help please [Re: mushpunx]
#21882718 - 07/01/15 12:25 PM (8 years, 6 months ago) |
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Quote:
mushpunx said:
Quote:
Mad Season said:
Quote:
FriedEgg said: I think he's referring to the fact that spore syringes are not sterile.
Since spores came from caps that fruited in open air. Cakes are pretty lenient and don't contaminate easily, but actually put a syringe under a microscope (they are for microscopy after all) and it has more than just cube spores in it. Bacteria and other stuff is there too.
I really had no idea why people would talk about contam problems so much when I first started growing, I have NevER had a cake contaminate! I even got away with plenty of bulk growns made with grain spawn injected with spores. But man once you start getting problems its an endless battle 
It wasnt untill I started working with agar that I saw what people meant when they say it is quite rare for a spore syringe to be truly clean. More frequently then not when I germinate spores on agar I have to isolate away from bacteria, sometimes mold colonies. All stuff in your syringe that you might get away with on a BRF cake but it will certainly not fly on grain.
So what Im saying is you are more likely to get contams into yiur grain jars from the syringe itself than opening the jar to squirt the spores in (under proper conditions in still air box). So the little bit of protection it might offer isnt really worth the experience you will get learning sterile procedure. Its essentially the same motions yiu will need for agar work and all the fun stuff involved in making grain spawn 
To the OP: you CAN have very sucessfull grows by syringe to grain so I hope this 1 turns out fine but look into agar for next grow!
Will definitely move onto Agar once the rye gets going. I go the extra mile when keeping clean...all grows to this point have been great, just wanna make sure I'm using the right set up for the right tek
Edited by Mushie23 (07/01/15 12:26 PM)
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Mushie23
Entheogens



Registered: 11/19/10
Posts: 789
Loc: PNW
Last seen: 7 years, 10 months
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Re: Injecton ports, trusted/experienced help please [Re: OPB]
#21882731 - 07/01/15 12:31 PM (8 years, 6 months ago) |
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Quote:
OPB said: Thanks for clearing that up. I hope this was useful for the OP and not just a thread jack...
All of this was very helpful! Will take the injection port lids off the BRF jars and swap them over to the RYE jars for the next round. Mostly agitated about losing a good chunk of a syringe in the BRF jars, lesson learned.
Sorry for the consecutive posts, but wanted to respond to a certain few. It's little tips like these that will help tighten things up
-------------------- Eat Mushrooms----plug into life and nature.
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Injecton ports, trusted/experienced help please [Re: Mushie23]
#21883022 - 07/01/15 02:02 PM (8 years, 6 months ago) |
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as long as they have a filtered GE hole its fine. you dont have the use the 4 inoc ports/ SHIPs just because they're there!
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