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oysterlover
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What do you do after agar?
#21877032 - 06/30/15 08:41 AM (8 years, 6 months ago) |
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I've been doing this for a few years now, and after taking a break I've come back to it. I've got several strains isolated on agar, and am happy with my collection so far. I'm wondering which way to go next.
I've typically dropped wedges into rye grain jars, but I'm looking for something better. I have a few jars with small amounts of grain colonizing and I'm hoping to make a GLI from them once they are done.
What is your typical next step after agar?
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matsc
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Re: What do you do after agar? [Re: oysterlover]
#21877148 - 06/30/15 09:11 AM (8 years, 6 months ago) |
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When im growing edibles, what I do is take a syringe of sterile water, squirt a mL or so on the plate, and scrape up a bunch of mycelia with the needle. Then I suck the lot of it back into the syringe, and voila, instant "liquid culture" that is much more stable over time than a true LC syringe would be (no worrying about needle port clogging from growth!)
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oysterlover
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Re: What do you do after agar? [Re: matsc]
#21877350 - 06/30/15 10:09 AM (8 years, 6 months ago) |
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That's not a bad idea. I've heard of doing that with grain jars, but not with a petri dish...good idea!
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matsc
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Re: What do you do after agar? [Re: oysterlover]
#21877727 - 06/30/15 11:45 AM (8 years, 6 months ago) |
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It takes a little practice to avoid chunking up the agar completely and clogging the needle, but with a light tough you can mostly just pull away mycelia and go from there.
...Or cheat and use a larger bore needle, which I usually end up doing. On the plus side, after doing so, the plate can recover and re-grow more hyphae! (Assuming there are still nutrients available anyway)
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mesq
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Re: What do you do after agar? [Re: matsc]
#21879473 - 06/30/15 06:26 PM (8 years, 6 months ago) |
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36fuckin5
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Re: What do you do after agar? [Re: mesq]
#21882158 - 07/01/15 09:36 AM (8 years, 6 months ago) |
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Oh, that's why this new "LI" term has been popping up and annoying the piss out of me. Liquid culture IS liquid inoculant.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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matsc
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Re: What do you do after agar? [Re: 36fuckin5]
#21882587 - 07/01/15 11:34 AM (8 years, 6 months ago) |
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Yes but liquid inoculate isn't necessarily liquid culture. LI (in the context im used to anyway), there isnt any metabolic activity, its just hyphae in liquid. LC on the other hand is actively growing/metabolizing/etc'ing in its liquid. Of course there are just my interpretation and I could be miles off base
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36fuckin5
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Re: What do you do after agar? [Re: matsc]
#21882612 - 07/01/15 11:44 AM (8 years, 6 months ago) |
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So call it a mycelial suspension, not LI. Those terms are gonna get noobs confused.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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matsc
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Re: What do you do after agar? [Re: 36fuckin5]
#21882703 - 07/01/15 12:17 PM (8 years, 6 months ago) |
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Quote:
36fuckin5 said: So call it a mycelial suspension, not LI. Those terms are gonna get noobs confused.
Works for me!
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Toadstool5
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Re: What do you do after agar? [Re: matsc]
#21887394 - 07/02/15 10:44 AM (8 years, 6 months ago) |
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Quote:
When im growing edibles, what I do is take a syringe of sterile water, squirt a mL or so on the plate, and scrape up a bunch of mycelia with the needle. Then I suck the lot of it back into the syringe, and voila, instant "liquid culture"
Would this work with a small agar square in a small centrifuge tube? I have bears head tooth, popcorn oyster, and phoenix oyster squares and i was thinking i would grow them out in a pint jar of rye then G2G into quart jars of rye. Im concerned the tiny piece of mycellium will be killed if i break it up into a mycellial suspension for inoculation.

Perhaps i should grow them out on agar when i have the supplies and then try your method. I do have a shiitake 75 plate i can play with for now but it probably wont fruit with how hot it is right now in CA
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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oysterlover
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Re: What do you do after agar? [Re: matsc]
#21887465 - 07/02/15 11:07 AM (8 years, 6 months ago) |
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Agreed. I was thinking of making a GLI or Grain Liquid Innoculant. I have two jars with approx 1 cup retrilized rye. Both are growing PE6 right now, and when fully colonized I plan to add water, shake vigorously, and suck up the liquid innoculant to use on other jars / bags.
-------------------- Contaminate is a verb. Contaminant is a noun.
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matsc
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Re: What do you do after agar? [Re: Toadstool5]
#21887528 - 07/02/15 11:26 AM (8 years, 6 months ago) |
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Toadstool, with a wedge that tiny I dont think I'd personally risk it. Adding the water might swell it a bit as it re-hydrates, but even then, that is quite small.
However.
The trick would still work in principle. You would end up with a very dilute inoculate, but mycelia can survive a surprising amount of abuse and rebound (or at least, a surprising amount of hyphae can survive undamaged and make it appear as such). If you dont have access to agar to grow out the wedge by all means, give it a try. Another option would be to add a bit of liquid media to the tube to allow it to grow out some, then draw from that. However, that gives you absolutely zero room for error and contamination control.
Weigh your options and how much you are willing to risk your wedge and decide if you want to go ahead now or wait till you have more to work with.
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Toadstool5
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Re: What do you do after agar? [Re: matsc]
#21887545 - 07/02/15 11:32 AM (8 years, 6 months ago) |
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Thats why i'm so nervous with how i want to procede. They are such cute little things 
Quote:
Another option would be to add a bit of liquid media to the tube to allow it to grow out some, then draw from that.
I will either try adding some LC or simply transfer to a small jar of grain and make the GLI everyone talks about instead of doing G2G.
Even if i do lose them i will simply trade for more or purchase more through a sponser. It will be fun!
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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matsc
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Re: What do you do after agar? [Re: Toadstool5]
#21887613 - 07/02/15 11:52 AM (8 years, 6 months ago) |
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Also dont be afraid of trying to fruit shiitake in warm weather. Just play around with it. You may get lucky.
I (half accidentally) managed to develop a strain of it that we ("we" being a program at my school that supplies local chefs) grow here in Tucson that fruits pretty much year round. Not particularly prolific fruiting year round, mind you, but it will still produce.
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Toadstool5
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Re: What do you do after agar? [Re: matsc]
#21887641 - 07/02/15 12:01 PM (8 years, 6 months ago) |
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Yeah and the shroomerite i traded for said shiitake 75 is more reliable in warmer conditions compared to some of the other varieties so I should probably practice my sectoring and transfers some on it before messing with the more limited cultures.
If you can do it in Tucson then i can do it where I am 
Thanks for info guys! Really helped me decide how i am going get my cultures going. Im not currently actively growing so I have very limited resources but want to get started up again.
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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kushroom



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Re: What do you do after agar? [Re: matsc]
#21892033 - 07/03/15 10:32 AM (8 years, 6 months ago) |
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Quote:
matsc said: Yes but liquid inoculate isn't necessarily liquid culture. LI (in the context im used to anyway), there isnt any metabolic activity, its just hyphae in liquid. LC on the other hand is actively growing/metabolizing/etc'ing in its liquid. Of course there are just my interpretation and I could be miles off base 
From what i understand thatd be the text book definition of it.
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36fuckin5
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Re: What do you do after agar? [Re: kushroom]
#21894960 - 07/03/15 11:08 PM (8 years, 6 months ago) |
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But LC is also a liquid inoculant, as is a spore solution. It's a misleading term. Mycelial suspension can really only refer to a LC, or more often, a suspension of mycelium in water.
Adding new terms for old techniques only confuses noobs.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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