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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Re: Got some new supplies to work with [Re: 2shoes]
#22029529 - 08/01/15 05:02 PM (8 years, 5 months ago) |
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Word,
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bakenast
Muffen Stuffer


Registered: 03/14/11
Posts: 920
Last seen: 1 year, 25 days
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Re: Got some new supplies to work with [Re: 2shoes]
#22029700 - 08/01/15 05:40 PM (8 years, 5 months ago) |
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Quote:
newbie2shoes said: I use alcohol to wipe the ship the flame needle and stick the needle though the alcohol wipe and into the ship if that makes sense. Ive had 3 of my 20 rye jars contaminate. 
maybe would have 0 contam out of 20 if you would of skipped the needle through the wipe?
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2shoes
The anti-agar



Registered: 04/07/11
Posts: 3,124
Loc: Not in a SAB
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Re: Got some new supplies to work with [Re: bakenast]
#22029825 - 08/01/15 06:07 PM (8 years, 5 months ago) |
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Or if I used agar instead of MS
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Got some new supplies to work with [Re: 2shoes]
#22029839 - 08/01/15 06:10 PM (8 years, 5 months ago) |
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well assuming that the ship was sterilized and was in a sterile environment, using alcohol would only make it dirtier. However like you said, agar is the actual way to go about it.
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2shoes
The anti-agar



Registered: 04/07/11
Posts: 3,124
Loc: Not in a SAB
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Re: Got some new supplies to work with [Re: Mad Season]
#22030041 - 08/01/15 06:47 PM (8 years, 5 months ago) |
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Quote:
Mad Season said:
well assuming that the ship was sterilized and was in a sterile environment, using alcohol would only make it dirtier. However like you said, agar is the actual way to go about it. 
It was sterilized but taken out of the sterile environment (PC) for inoculation.
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Re: Got some new supplies to work with [Re: Mad Season]
#22030511 - 08/01/15 08:38 PM (8 years, 5 months ago) |
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I still haven't found agar in stores yet. Going to get it on amazon I think. Along with petras. Got a huge fucking bag of vermiculite today though. Rye flower cakes are going to be made tomorrow.
Edited by YaMoonSun (08/01/15 08:44 PM)
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2shoes
The anti-agar



Registered: 04/07/11
Posts: 3,124
Loc: Not in a SAB
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Re: Got some new supplies to work with [Re: YaMoonSun]
#22030543 - 08/01/15 08:52 PM (8 years, 5 months ago) |
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2:1:1 Verm:flour:water
Mix Verm and water first then add the flour and some coffee if you have some.
Rye flour is like $4 for 5lbs at the supermarket near me
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Re: Got some new supplies to work with [Re: 2shoes]
#22030571 - 08/01/15 09:01 PM (8 years, 5 months ago) |
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Bought mine from the local farmers market, was like $7, idk how big the bag was. At least 2lb I'd like to imagine.
Edited by YaMoonSun (08/01/15 09:10 PM)
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Re: Got some new supplies to work with [Re: YaMoonSun]
#22038334 - 08/03/15 04:35 PM (8 years, 5 months ago) |
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It seems the perlite in the terrarium is developing algae. Should I kill it?
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: Got some new supplies to work with [Re: YaMoonSun]
#22038431 - 08/03/15 04:57 PM (8 years, 5 months ago) |
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Won't hurt the cakes or the perlite but yes.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Re: Got some new supplies to work with [Re: taGyo]
#22038496 - 08/03/15 05:13 PM (8 years, 5 months ago) |
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I was going to ask if it would produce oxygen and help out the cakes, but after reading this, I'm guessing no.
Quote:
Like all green plants, algae produce oxygen during the daylight hours as a by-product of photosynthesis. This is usually a major source of oxygen in fish ponds. In darkness, however, all plants consume oxygen, including algae. Algae blooms in natural water bodies or fish ponds normally produce much more oxygen in the daylight than they consume during the night, but some situations reduce the amount of oxygen a bloom produces without reducing its nighttime oxygen consumption. Trace minerals or nutrients needed by the algae are occasionally used up, causing some or even all, of the bloom to die back temporarily. The resulting bacterial decomposition and loss of normal oxygen production can lead to oxygen depletions and fish kills. Pond water generally changes from a deep green to black, gray, brown or clear after a phytoplankton die-off. An additional problem caused by dense blooms, especially in excessively deep ponds, is stratification. As mentioned elsewhere, stratification involves layering of the pond water into warm, oxygen-producing upper zones and cool, oxygen-consuming bottom waters. Shading caused by dense blooms limits photosynthesis and dissolved oxygen levels at the pond bottom, resulting in a buildup of potentially toxic compounds, even in aerated ponds. This situation can lead to physiological stress, reduced fish growth, and even fish kills if bottom waters are mixed too rapidly with the rest of the pond. This type of mixing, referred to as a turnover, occurs when cool rain water or heavy wind on the pond surface breaks down layering patterns. Turnovers are often observed in natural waters and ponds in the fall or spring after severe weather disturbances. The potential for a turnover can be detected by an increasing temperature difference between a pond’s bottom layers and its surface waters. If a turnover is likely, prepare for aeration when necessary. Algae blooms also respond to changes in the weather, and the denser the bloom, the more severe the response can be. Photosynthesis slows down under cloudy conditions, and, as a result, oxygen production decreases. Extremely calm days may also reduce photosynthesis and oxygen production, even under sunny conditions, by preventing phytoplankton in the middle layers of the pond from mixing near the brighter surface. In summer, oxygen problems may arise because of a simple physical property of water. The warmer the water, the less dissolved oxygen it can hold. When a dense bloom produces a surplus of oxygen on a summer afternoon, the oxygen will not stay in solution and escapes into the atmosphere. During the night, the bloom attempts to take more oxygen out of the water than what remains from daytime photosynthesis. When this occurs, dissolved oxygen levels approach zero.
Edited by YaMoonSun (08/03/15 05:14 PM)
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: Got some new supplies to work with [Re: YaMoonSun]
#22038499 - 08/03/15 05:15 PM (8 years, 5 months ago) |
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Are you Blackdust by chance?
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Re: Got some new supplies to work with [Re: taGyo]
#22038523 - 08/03/15 05:18 PM (8 years, 5 months ago) |
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No?
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: Got some new supplies to work with [Re: YaMoonSun]
#22038529 - 08/03/15 05:19 PM (8 years, 5 months ago) |
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Yeah, I would wash it off.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
Edited by taGyo (08/03/15 05:19 PM)
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Re: Got some new supplies to work with [Re: taGyo]
#22038545 - 08/03/15 05:21 PM (8 years, 5 months ago) |
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Will do, I'll have to see if there's enough hydrogen peroxide.
This page needs more pictures, here's the substrates I prepped a few days ago.
 Golden Teacher, Treasure Coast, ATL#7, Golden Teacher
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Re: Got some new supplies to work with [Re: YaMoonSun]
#22117452 - 08/20/15 04:15 PM (8 years, 5 months ago) |
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Only two one of those jars is showing signs of colonization, so I made a new golden teacher syringe and inoculated the other two again, and made the biggest cake I've ever made.
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Re: Got some new supplies to work with [Re: YaMoonSun]
#22128068 - 08/22/15 05:55 PM (8 years, 5 months ago) |
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Guess what happens when you try to colonize outdoors?
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: Got some new supplies to work with [Re: YaMoonSun]
#22128674 - 08/22/15 09:09 PM (8 years, 5 months ago) |
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How're your jars doing?
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Re: Got some new supplies to work with [Re: taGyo]
#22128698 - 08/22/15 09:17 PM (8 years, 5 months ago) |
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Didn't see all the maggots in that jar? One of the small jars is showing life, and it's a bit early to tell on the other two.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: Got some new supplies to work with [Re: YaMoonSun]
#22128701 - 08/22/15 09:17 PM (8 years, 5 months ago) |
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I meant the ones without the maggots 
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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