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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
Re: Got some new supplies to work with [Re: 2shoes]
    #22029529 - 08/01/15 05:02 PM (8 years, 5 months ago)

Word, :cheers:


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Offlinebakenast
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Registered: 03/14/11
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Last seen: 1 year, 25 days
Re: Got some new supplies to work with [Re: 2shoes]
    #22029700 - 08/01/15 05:40 PM (8 years, 5 months ago)

Quote:

newbie2shoes said:
I use alcohol to wipe the ship the flame needle and stick the needle though the alcohol wipe and into the ship if that makes sense.
Ive had 3 of my 20 rye jars contaminate. :shrug:




maybe would have 0 contam out of 20 if you would of skipped the needle through the wipe?


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Invisible2shoes
The anti-agar
Male


Registered: 04/07/11
Posts: 3,124
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Re: Got some new supplies to work with [Re: bakenast]
    #22029825 - 08/01/15 06:07 PM (8 years, 5 months ago)

Or if I used agar instead of MS :goodluckwiththat2:


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InvisibleMad Season
hookers and blackjack
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Registered: 09/16/12
Posts: 12,666
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Re: Got some new supplies to work with [Re: 2shoes]
    #22029839 - 08/01/15 06:10 PM (8 years, 5 months ago)

:lol: well assuming that the ship was sterilized and was in a sterile environment, using alcohol would only make it dirtier. However like you said, agar is the actual way to go about it. :lol:


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Invisible2shoes
The anti-agar
Male


Registered: 04/07/11
Posts: 3,124
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Re: Got some new supplies to work with [Re: Mad Season]
    #22030041 - 08/01/15 06:47 PM (8 years, 5 months ago)

Quote:

Mad Season said:
:lol: well assuming that the ship was sterilized and was in a sterile environment, using alcohol would only make it dirtier. However like you said, agar is the actual way to go about it. :lol:




It was sterilized but taken out of the sterile environment (PC) for inoculation.


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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
Re: Got some new supplies to work with [Re: Mad Season]
    #22030511 - 08/01/15 08:38 PM (8 years, 5 months ago)

I still haven't found agar in stores yet. Going to get it on amazon I think. Along with petras.
Got a huge fucking bag of vermiculite today though. Rye flower cakes are going to be made tomorrow.


Edited by YaMoonSun (08/01/15 08:44 PM)


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Invisible2shoes
The anti-agar
Male


Registered: 04/07/11
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Re: Got some new supplies to work with [Re: YaMoonSun]
    #22030543 - 08/01/15 08:52 PM (8 years, 5 months ago)

2:1:1 Verm:flour:water

Mix Verm and water first then add the flour and some coffee if you have some.

Rye flour is like $4 for 5lbs at the supermarket near me :shrug:


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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
Re: Got some new supplies to work with [Re: 2shoes]
    #22030571 - 08/01/15 09:01 PM (8 years, 5 months ago)

Bought mine from the local farmers market, was like $7, idk how big the bag was. At least 2lb I'd like to imagine.


Edited by YaMoonSun (08/01/15 09:10 PM)


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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
Re: Got some new supplies to work with [Re: YaMoonSun]
    #22038334 - 08/03/15 04:35 PM (8 years, 5 months ago)

It seems the perlite in the terrarium is developing algae. Should I kill it? :whatdoyouthink:


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OfflinetaGyo
Strainiac/AMU
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Registered: 10/16/14
Posts: 18,802
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Re: Got some new supplies to work with [Re: YaMoonSun]
    #22038431 - 08/03/15 04:57 PM (8 years, 5 months ago)

Won't hurt the cakes or the perlite but yes.


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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
Re: Got some new supplies to work with [Re: taGyo]
    #22038496 - 08/03/15 05:13 PM (8 years, 5 months ago)

I was going to ask if it would produce oxygen and help out the cakes, but after reading this, I'm guessing no.

Quote:

Like all green plants, algae produce oxygen during the daylight hours as a by-product of photosynthesis. This is usually a major source of oxygen in fish ponds. In darkness, however, all plants consume oxygen, including algae. Algae blooms in natural water bodies or fish ponds normally produce much more oxygen in the daylight than they consume during the night, but some situations reduce the amount of oxygen a bloom produces without reducing its nighttime oxygen consumption. Trace minerals or nutrients needed by the algae are occasionally used up, causing some or even all, of the bloom to die back temporarily. The resulting bacterial decomposition and loss of normal oxygen production can lead to oxygen depletions and fish kills. Pond water generally changes from a deep green to black, gray, brown or clear after a phytoplankton die-off. An additional problem caused by dense blooms, especially in excessively deep ponds, is stratification. As mentioned elsewhere, stratification involves layering of the pond water into warm, oxygen-producing upper zones and cool, oxygen-consuming bottom waters. Shading caused by dense blooms limits photosynthesis and dissolved oxygen levels at the pond bottom, resulting in a buildup of potentially toxic compounds, even in aerated ponds. This situation can lead to physiological stress, reduced fish growth, and even fish kills if bottom waters are mixed too rapidly with the rest of the pond. This type of mixing, referred to as a turnover, occurs when cool rain water or heavy wind on the pond surface breaks down layering patterns. Turnovers are often observed in natural waters and ponds in the fall or spring after severe weather disturbances. The potential for a turnover can be detected by an increasing temperature difference between a pond’s bottom layers and its surface waters. If a turnover is likely, prepare for aeration when necessary. Algae blooms also respond to changes in the weather, and the denser the bloom, the more severe the response can be. Photosynthesis slows down under cloudy conditions, and, as a result, oxygen production decreases. Extremely calm days may also reduce photosynthesis and oxygen production, even under sunny conditions, by preventing phytoplankton in the middle layers of the pond from mixing near the brighter surface. In summer, oxygen problems may arise because of a simple physical property of water. The warmer the water, the less dissolved oxygen it can hold. When a dense bloom produces a surplus of oxygen on a summer afternoon, the oxygen will not stay in solution and escapes into the atmosphere. During the night, the bloom attempts to take more oxygen out of the water than what remains from daytime photosynthesis. When this occurs, dissolved oxygen levels approach zero.




Edited by YaMoonSun (08/03/15 05:14 PM)


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OfflinetaGyo
Strainiac/AMU
Male User Gallery


Registered: 10/16/14
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Re: Got some new supplies to work with [Re: YaMoonSun]
    #22038499 - 08/03/15 05:15 PM (8 years, 5 months ago)

Are you Blackdust by chance?


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
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Dominus fortunae meae sum


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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
Re: Got some new supplies to work with [Re: taGyo]
    #22038523 - 08/03/15 05:18 PM (8 years, 5 months ago)

No? :shrug:


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OfflinetaGyo
Strainiac/AMU
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Registered: 10/16/14
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Re: Got some new supplies to work with [Re: YaMoonSun]
    #22038529 - 08/03/15 05:19 PM (8 years, 5 months ago)

Yeah, I would wash it off.


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Gyo's Better Grows
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Edited by taGyo (08/03/15 05:19 PM)


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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
Re: Got some new supplies to work with [Re: taGyo]
    #22038545 - 08/03/15 05:21 PM (8 years, 5 months ago)

Will do, I'll have to see if there's enough hydrogen peroxide. :super:

This page needs more pictures, here's the substrates I prepped a few days ago.

Golden Teacher, Treasure Coast, ATL#7, Golden Teacher


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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
Re: Got some new supplies to work with [Re: YaMoonSun]
    #22117452 - 08/20/15 04:15 PM (8 years, 5 months ago)

Only two one of those jars is showing signs of colonization, so I made a new golden teacher syringe and inoculated the other two again, and made the biggest cake I've ever made.


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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
Re: Got some new supplies to work with [Re: YaMoonSun]
    #22128068 - 08/22/15 05:55 PM (8 years, 5 months ago)

Guess what happens when you try to colonize outdoors?


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OfflinetaGyo
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Registered: 10/16/14
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Re: Got some new supplies to work with [Re: YaMoonSun]
    #22128674 - 08/22/15 09:09 PM (8 years, 5 months ago)

How're your jars doing?


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Gyo's Better Grows
TNF Q&A :rockon:
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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
Re: Got some new supplies to work with [Re: taGyo]
    #22128698 - 08/22/15 09:17 PM (8 years, 5 months ago)

Didn't see all the maggots in that jar?
One of the small jars is showing life, and it's a bit early to tell on the other two.


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OfflinetaGyo
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Re: Got some new supplies to work with [Re: YaMoonSun]
    #22128701 - 08/22/15 09:17 PM (8 years, 5 months ago)

I meant the ones without the maggots :lol:

:thumbup:


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Gyo's Better Grows
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