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OfflineJsneeb
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Registered: 04/05/15
Posts: 109
Last seen: 6 years, 10 months
Jar shows no growth, can I re-inoculate? (PAN CYAN)
    #21874099 - 06/29/15 03:33 PM (8 years, 6 months ago)

Jar shows no growth, can I re-inoculate? (PAN CYAN)

So I PC'd 3 1 quart jars with grain and a handfull of hpoo. I head this sia good mix to help it colonize straw quickly.

Two I inoulated via G2G from a mini 4 oz jar of colonized grain.
The other one I used some LI I had left over.

Here are all the details.
3 identical jars PC for 45 minutes. Left overnight, cooled, then PC for another 45 minutes the next morning, 5 hours later. ( I started the PC too late and was seriously tired, Was afraid I would fall asleep with the PC still running and run it dry or blow my house up, so i shut it down early.)
2 mini 4 oz jars mixed into the quart jars. Super fast growth, fully colonized in 3 days.
3rd jar was not inoculated with a mini 4 oz because I started 3 and one contamed so I tossed it out.
I used some pan Cyan LI i made about 2 weeks prior. I know it worked and it was clean because I used it to start some more agar plates which have since grown and were used to begin the 4oz jars used for the above operation.

The Li was stored in the fridge which i know gets a bit cold. Sometimes my milk is a bit icy.

stored for 2 weeks. needle flamed and injected into the 3rd jar. used about 5 cc's All that was left.

So far no growth.

Should I toss it or can I PC the jar and re-inoculate it with agar wedge of pan Cyan?


--------------------
Read my trip report :smile:

Titled, Mushrooms, Thirsty Water Bottle, And Boiling Peanut-butter?

http://www.reddit.com/r/shrooms/comments/37z9rp/mushrooms_thirsty_water_bottle_and_boiling/


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OfflineDensePlacebo
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Registered: 05/14/15
Posts: 186
Loc: canada Flag
Last seen: 6 years, 4 months
Re: Jar shows no growth, can I re-inoculate? (PAN CYAN) [Re: Jsneeb]
    #21874127 - 06/29/15 03:41 PM (8 years, 6 months ago)

I would toss it. There's probably a reason it didn't work and even if it wasn't the grain, its still better to play it safe. But wait and see what more experienced peoples say.


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Alright then, picture this if you will:
10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51.
Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this.
Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping...
Holy fucking shit!




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OfflineJsneeb
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Registered: 04/05/15
Posts: 109
Last seen: 6 years, 10 months
Re: Jar shows no growth, can I re-inoculate? (PAN CYAN) [Re: DensePlacebo]
    #21874236 - 06/29/15 04:13 PM (8 years, 6 months ago)

I have two thought on this myself.
Firstly my tray needs 3 quarts of spawn to be full and have enough thickness.
I could change my spawn to bulk ratio up a bit to make up for it but making up for 30 % isn't going to happen.

So if it can still be used then cool.

However, now that I think about it, if there's a chance that jar has contams then I probably don't want to mix it in with my know good jars in the tray anyway, else I'll loose everything?

I'l probably try to block of part of the tray or go buy something smaller and just do 1.5 quarts spawn to 2 quarts hpoo/straw.

My jars are filled 75% so I only have 1.5 quarts spawn right now.


--------------------
Read my trip report :smile:

Titled, Mushrooms, Thirsty Water Bottle, And Boiling Peanut-butter?

http://www.reddit.com/r/shrooms/comments/37z9rp/mushrooms_thirsty_water_bottle_and_boiling/


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OfflineShroominMe
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Registered: 05/03/15
Posts: 525
Last seen: 8 years, 3 months
Re: Jar shows no growth, can I re-inoculate? (PAN CYAN) [Re: Jsneeb]
    #21882094 - 07/01/15 09:13 AM (8 years, 6 months ago)

I've had 100% success re-inoculating jars which didn't colonize during the first attempt.


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