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OfflineAl Capone
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Registered: 06/29/15
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Last seen: 8 years, 6 months
First time, trying to learn
    #21873278 - 06/29/15 11:47 AM (8 years, 6 months ago)

Well, this is my first post on shroomery, after stumbling to this site for various reasons over the years..

I've always loved mycology and botany, and try to learn as much as I can about them. What better way than trying my first real mushroom cultivation??

This is what I've gathered so far and want to know if i'm missing anything important:

Boil Grain seed, let dry.
Fill jars with grain, create FAE hole in lids and seal with Cotton? bandaid?
Steam sterilize jars containing grain for ~1 hour, let cool.
At a sterile work station, cut living tissue from inside stem of mushroom. (i will be using an oyster mushroom simply for learning purposes and legality)
Quickly open lid, drop in live tissue, and close up jars.
Put them into warm, humid, dark, spot until consolidation.

Once full consolidated, Would i be able to break small pieces off the mycelium, insert into new, sterilized grain jars to get new jars inoculated.
Then, use remaining mycelium inside a make shift terrarium for fruiting.


Well thats my game plan as of now, anything I should change or look into?
Again, this entire set up is purely to learn Sterilization, Inoculation, and whatever else. I have fruited mycelium before, but never cultivated my own


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InvisiblebodhisattaMDiscordReddit
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Re: First time, trying to learn [Re: Al Capone]
    #21873382 - 06/29/15 12:08 PM (8 years, 6 months ago)

Follow a grain prep for your grain.
Lids need gas exchange not fae follow a lid tek
You have to pressure sterilize grain at very least 90 m if you steam sterilize 8+ hours. Pressure cooker is a must for grain
After inoculation of jars put them anywhere but a dark humid place. Jars don't give a fuck about the humidity outside of the jar and light is good for them at all stages.

Once fully colonized you don't use a piece of mycelium to re- inoculate jars. At least not like that, unless you want to grow nasty mold.


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OfflineAl Capone
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Registered: 06/29/15
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Re: First time, trying to learn [Re: bodhisatta]
    #21873512 - 06/29/15 12:46 PM (8 years, 6 months ago)

Quote:

bodhisatta said:
Follow a grain prep for your grain.
Lids need gas exchange not fae follow a lid tek
You have to pressure sterilize grain at very least 90 m if you steam sterilize 8+ hours. Pressure cooker is a must for grain
After inoculation of jars put them anywhere but a dark humid place. Jars don't give a fuck about the humidity outside of the jar and light is good for them at all stages.

Once fully colonized you don't use a piece of mycelium to re- inoculate jars. At least not like that, unless you want to grow nasty mold.




Ah, i must be confusing FAE and gas exchange, ill look into each one separately. As for PC vs Steam, everything I read said steam will suffice unless trying to mass inoculate.
I realize the jars dont care about whats outside of them, but i figured with the Gas exchange it would make a difference, guess i was completely wrong.

I saw multiple guides transferring mycelium to fresh jars to keep producing more. I figured this way would be less "fool-proof" but a lot simpler for the learning stages.

I obviously have a lot more to learn, but hey thats why i posted this before buying everything! :smile: Thanks for the response

edit: also, I dont have access to any spore syringes and don't want to buy any until I get my tek down (thats when i try cubes!) i was going to just use everything easily accessible, such as live mushroom tissue and creating my own spore syringes


Edited by Al Capone (06/29/15 12:49 PM)


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OfflineHamHead
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Re: First time, trying to learn [Re: Al Capone]
    #21873549 - 06/29/15 12:56 PM (8 years, 6 months ago)

Look into agar. You can take tissue sample to agar and drop wedges into your grain jars. No need for a syringe.


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Invisiblenatedawgnow
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Re: First time, trying to learn [Re: Al Capone]
    #21873580 - 06/29/15 01:07 PM (8 years, 6 months ago)

What you are referring to is a grain to grain transfer. After you have a jar that is fully colonized, you can transfer some of the colonized grain by dumping it into another freshly sterilized jar of grain. Of course all of this needs to be done in a sterile work environment like a still air box or in front of a flowhood


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InvisiblebodhisattaMDiscordReddit
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Re: First time, trying to learn [Re: natedawgnow]
    #21873799 - 06/29/15 02:12 PM (8 years, 6 months ago)

To steam sterilize whole kernel grain you have to steam without pressure for a bare minimum of 8 hours. So I suppose it will suffice but fuck that. You may steam PF(brf) cakes without pressure

The second link in my signature will apply to both cubes and oysters aside from oysters likeing cooler temps


Edited by Trusted cuItivator (06/29/15 02:13 PM)


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OfflineAl Capone
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Registered: 06/29/15
Posts: 4
Last seen: 8 years, 6 months
Re: First time, trying to learn [Re: bodhisatta]
    #21873944 - 06/29/15 02:52 PM (8 years, 6 months ago)

Quote:

bodhisatta said:
To steam sterilize whole kernel grain you have to steam without pressure for a bare minimum of 8 hours. So I suppose it will suffice but fuck that. You may steam PF(brf) cakes without pressure

The second link in my signature will apply to both cubes and oysters aside from oysters likeing cooler temps




Thanks, i will check out your guides!
but I saw from multiple sources they washed the grains in hot water, boiled, strained, let them dry to the point of no outside moisture, put into glass jars, Steam/PC the jars for 1 hour. Seemed simple enough to follow, is there something flawed about it?


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Offlinespacechildo
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Re: First time, trying to learn [Re: Al Capone]
    #21874004 - 06/29/15 03:10 PM (8 years, 6 months ago)

Quote:

Al Capone said:
Quote:

bodhisatta said:
To steam sterilize whole kernel grain you have to steam without pressure for a bare minimum of 8 hours. So I suppose it will suffice but fuck that. You may steam PF(brf) cakes without pressure

The second link in my signature will apply to both cubes and oysters aside from oysters likeing cooler temps




Thanks, i will check out your guides!
but I saw from multiple sources they washed the grains in hot water, boiled, strained, let them dry to the point of no outside moisture, put into glass jars, Steam/PC the jars for 1 hour 90mins. Seemed simple enough to follow, is there something flawed about it?




there, fixed it to show you need a PC, that's whats "flawed" :super:


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InvisiblebodhisattaMDiscordReddit
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Re: First time, trying to learn [Re: spacechildo]
    #21874045 - 06/29/15 03:19 PM (8 years, 6 months ago)

yea even with a pressure cooker at 15psi 250F 1 hour is not enough time.

the guides you read wouldn't be guides I would read. how old are they like 20+ years and wrote by kids on youtube?


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