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Offlinepsilo cybe
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Registered: 03/20/15
Posts: 71
Last seen: 8 years, 4 months
First Mono (Harvest pics)
    #21871894 - 06/29/15 03:37 AM (8 years, 6 months ago)

Hi, just hoping for a bit of advice on my first tub.

inoculation
RR's Rye tek used,
500g total sub used across 4 jars ( w/ tyvek & injection port)
1.1 cc of MS B+ per jar
4 weeks from inoculation to full colonization @ around 75 deg F. This would have been far quicker but I made the mistake of shaking the jars immediately after inoculation.

spawning
300g aged hpoo,
682g Coir,(1L brick)
230g Vermiculite,(2L)
20g gypsum,
6ml coffee grounds,
500g spawn
spawn ratio just shy of 1:3

I managed to fuck up the pasteurization thanks to a crappy thermometer so my sub ended up with a light sterilization at around 190deg. Nevertheless, things are looking good for the moment.

Currently on day 11 and I'm wondering if it would be beneficial to incubate for a few more days due to my low spawn ratio. What do you think?



peace :peace:


Edited by psilo cybe (07/10/15 01:09 AM)


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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
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Re: First Mono, how's it looking? [Re: psilo cybe]
    #21871975 - 06/29/15 05:05 AM (8 years, 6 months ago)

So far so good, imo - Going to be a nice harvest ahead of you. :goodluck:


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Offlinerudeboi
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Registered: 05/17/14
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Re: First Mono, how's it looking? [Re: YaMoonSun]
    #21872111 - 06/29/15 06:25 AM (8 years, 6 months ago)

I would leave it for an additional day or so.

Looks good, congrads:greatjob:


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Offlinepsilo cybe
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Re: First Mono, how's it looking? [Re: YaMoonSun]
    #21872543 - 06/29/15 09:35 AM (8 years, 6 months ago)

Yeah I hope so man. As long as it pans out better than my last cake grow then I'm happy.


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Offlinepsilo cybe
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Re: First Mono, how's it looking? [Re: rudeboi]
    #21872546 - 06/29/15 09:36 AM (8 years, 6 months ago)

cheers dude, thought as much.


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Offlinepsilo cybe
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Re: First Mono, how's it looking? [Re: psilo cybe]
    #21872692 - 06/29/15 10:11 AM (8 years, 6 months ago)

I just checked some liquid culture jars that I made after my last harvest over a month ago and one of them has actually grown! I can't believe it, I had completely given up on them and thought I had fucked something up as it was my first attempt at LC.

Please let me know if this looks alright... is the yellow spot in the last pic just metabs or glucose/fructose or should I be worried about contam of some kind.
Also, how come this LC took almost 6 weeks to colonize?

Sorry for the all the Q's but I'm in unfamiliar territory here.



I've got some grains soaking now and was planning on going the G2G route with a bit of spawn leftover from my mono, but if this LC is viable then I will make the most of it.


Edited by psilo cybe (07/10/15 01:10 AM)


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Offlinepsilo cybe
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Re: First Mono, how's it looking? [Re: psilo cybe]
    #21874557 - 06/29/15 05:31 PM (8 years, 6 months ago)

bump


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: First Mono, how's it looking? [Re: psilo cybe]
    #21874606 - 06/29/15 05:39 PM (8 years, 6 months ago)

I would definitely put some of that LC on agar to make sure it is contaminant-free before using it on grains. :thumbup:


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Offlinepsilo cybe
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Re: First Mono, how's it looking? [Re: Psilosoulful]
    #21876390 - 06/30/15 02:24 AM (8 years, 6 months ago)

Quote:

Psilosoulful said:
I would definitely put some of that LC on agar to make sure it is contaminant-free before using it on grains. :thumbup:




good call, I'll check out some agar teks's


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Offlinepsilo cybe
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Re: First Mono, how's it looking? [Re: psilo cybe]
    #21882106 - 07/01/15 09:17 AM (8 years, 6 months ago)

I had a peek this morning and noticed pins so I went ahead and put the tub into fruiting. The weather is quite nice at the moment so the ambient temp is around 70. 74 with the T5's on, which isn't bad.




I decided to just go ahead and use the LC to inoc a few more jars. If there is any contam it's no big loss... I have plenty of grain and time to spare. I used 2.5cc per jar and gave the jars a light roll after.


The tissue sample used for this LC came from a random shroom in the first sizable cluster to appear in my last grow rather than the biggest / prettiest shroom so I'll be interested to see what happens if this colonizes.

Also, I have to say... fuck pf tek! Going grain & not using MS seemed somewhat daunting from the onset but the reality is that after a decent bit of prior research, even for a beginner, the whole process has been far easier, quicker & much more interesting.

peace


Edited by psilo cybe (07/10/15 01:10 AM)


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Offlinepsilo cybe
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Re: First Mono, how's it looking? [Re: psilo cybe]
    #21899599 - 07/05/15 03:08 AM (8 years, 6 months ago)

3 days since introduction to fruiting:



counted around 80 pins total 

:fuckinawesome:


Edited by psilo cybe (07/10/15 01:11 AM)


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Offlinepsilo cybe
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Re: First Mono, how's it looking? [Re: psilo cybe]
    #21899616 - 07/05/15 03:27 AM (8 years, 6 months ago)

Oh, and within those three days the LC inoculated jars have all got signs of contam :ooo: oh well. g2g it is - I think I'll take both prints and tissue samples this time round...


Edited by psilo cybe (07/05/15 03:27 AM)


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InvisibleFantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc: Flag
Re: First Mono, how's it looking? [Re: psilo cybe]
    #21899652 - 07/05/15 04:06 AM (8 years, 6 months ago)

Looks like they will be some nice & healthy fruitss!
:thumbup:


--------------------
Giving is all we have, for we're just grateful to be alive

:kratom:


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Offlinepsilo cybe
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Re: First Mono, how's it looking? [Re: Fantastic Mr. Fox]
    #21899889 - 07/05/15 07:00 AM (8 years, 6 months ago)

Quote:

Fantastic Mr. Fox said:
Looks like they will be some nice & healthy fruitss!
:thumbup:




Thanks man :laugh: if I end up with a canopy anything like the one in your sig I will be one happy dude!


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InvisibleFantastic Mr. Fox
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Registered: 02/12/15
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Re: First Mono, how's it looking? [Re: psilo cybe]
    #21899978 - 07/05/15 07:50 AM (8 years, 6 months ago)

:super:


--------------------
Giving is all we have, for we're just grateful to be alive

:kratom:


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Offlinepsilo cybe
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Registered: 03/20/15
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Re: First Mono, how's it looking? [Re: Fantastic Mr. Fox]
    #21908116 - 07/07/15 02:09 AM (8 years, 6 months ago)

6 days in:


Not the beautifully even pinset I was hoping for... overdoing the pasteurization and the shitty spawn ratio probably have something to do with that.
Still, pretty pleased so far :smile:


Edited by psilo cybe (07/10/15 01:12 AM)


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Offlinenewrook
Sucks at bulk
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Re: First Mono, how's it looking? [Re: psilo cybe]
    #21908323 - 07/07/15 05:07 AM (8 years, 6 months ago)

Quote:

psilo cybe said:
6 days in:







Not the beautifully even pinset I was hoping for... overdoing the pasteurization and the shitty spawn ratio probably have something to do with that.
Still, pretty pleased so far :smile:




Gonna be some fatties in there!! :dancingbacon:


--------------------

                                                                      THROW AWAY YOUR SGFC


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Offlinepsilo cybe
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Re: First Mono, how's it looking? [Re: newrook]
    #21908364 - 07/07/15 05:38 AM (8 years, 6 months ago)

Quote:

newrook said:
Gonna be some fatties in there!! :dancingbacon:




sure hope so man! :hahyeahwoo:


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Offlinepsilo cybe
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Registered: 03/20/15
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Re: First Mono, how's it looking? *DELETED* [Re: psilo cybe]
    #21913542 - 07/08/15 05:42 AM (8 years, 6 months ago)

Post deleted by psilo cybe

Reason for deletion: /



Edited by psilo cybe (07/10/15 03:31 AM)


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Offlinepsilo cybe
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Re: First Mono, how's it looking? [Re: psilo cybe]
    #21913553 - 07/08/15 05:49 AM (8 years, 6 months ago)

Also, am I good to simply air dry these babies at room temp?
Ambient average is about 70f, RH around 50%


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OfflineTayluh21
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Re: First Mono, how's it looking? [Re: psilo cybe]
    #21913587 - 07/08/15 06:11 AM (8 years, 6 months ago)

From what I've read simply air drying with a fan or something will but you a week or so, but for storage longer than that they should be dried until cracker dry. Which was unbelievably easy to achieve (just harvested my first) looks good though man. Congratulations


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
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Re: First Mono, how's it looking? [Re: Tayluh21]
    #21913635 - 07/08/15 06:35 AM (8 years, 6 months ago)

A dehydrator would be optimal for drying, but you can fan dry as well. Make sure after they are partially dried with the fan, you put them in a jar with some silica gels to get the last bits of moisture out, or they might lose a bit of potency over time.


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Offlinepsilo cybe
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Registered: 03/20/15
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Re: First Mono, how's it looking? [Re: Psilosoulful]
    #21917998 - 07/09/15 03:59 AM (8 years, 6 months ago)

Quote:

Psilosoulful said:
A dehydrator would be optimal for drying, but you can fan dry as well. Make sure after they are partially dried with the fan, you put them in a jar with some silica gels to get the last bits of moisture out, or they might lose a bit of potency over time.




Thanks for the input man, I actually managed to get hold of a small space heater today (fan type) so I'm using that on the lowest setting. I will definitely invest in a dehydrator in the future but for the moment this should do. I also have some of those large 50g silica sachets at my disposal.



Picked a few this morning. My SAB is currently disinfecting in preparation for my first G2G.


Edited by psilo cybe (07/10/15 03:40 AM)


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Offlinepsilo cybe
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Re: First Mono, how's it looking? [Re: psilo cybe]
    #21918028 - 07/09/15 04:21 AM (8 years, 6 months ago)

some prints and the g2g jars:


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Offlinepsilo cybe
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Re: First Mono, how's it looking? [Re: psilo cybe]
    #21922868 - 07/10/15 01:07 AM (8 years, 6 months ago)

That's the first flush fully cropped (:
The tub is currently being dunked in preparation for round two.




Edited by psilo cybe (07/10/15 03:38 AM)


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Offlinemycomaniac1402
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Re: First Mono, how's it looking? [Re: psilo cybe]
    #21922913 - 07/10/15 01:45 AM (8 years, 6 months ago)

Not a bad little flush you got there :thumbup: dont be discouraged man some flushes arent wall to wall and some are. You got some great things goin on , what flush is the tub on ? I like the pic 3 posts above with the fat caps !


--------------------


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Offlinepsilo cybe
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Re: First Mono, how's it looking? [Re: mycomaniac1402]
    #21923029 - 07/10/15 03:28 AM (8 years, 6 months ago)

Quote:

mycomaniac1402 said:
Not a bad little flush you got there :thumbup: dont be discouraged man some flushes arent wall to wall and some are. You got some great things goin on , what flush is the tub on ? I like the pic 3 posts above with the fat caps !




Cheers man! Just the first flush so far. The sub just finished its 11 hr soak and is back under lights. Any tips for the second flush?


Edited by psilo cybe (07/10/15 04:03 AM)


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InvisibleFantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc: Flag
Re: First Mono, how's it looking? [Re: psilo cybe]
    #21923219 - 07/10/15 06:01 AM (8 years, 6 months ago)

11hr soak??!!
:eek:
Never soak bulk longer than 3hrs..

Also, you want to let your substrate sorta dry out after each flush before dunking for 2-3hrs...

That being said, your substrate is probably water logged.


--------------------
Giving is all we have, for we're just grateful to be alive

:kratom:


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Offlinepsilo cybe
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Re: First Mono, how's it looking? [Re: Fantastic Mr. Fox]
    #21923245 - 07/10/15 06:19 AM (8 years, 6 months ago)

Ah shit I read a few posts that recommended up to 24 hrs...
The sub was really dry prior to soaking, I let it drain pretty well after but no doubt it's well over field capacity.


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