Home | Community | Message Board

Magic Mushrooms Zamnesia
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Buy Bali Kratom Powder   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinemadchemist1
Stranger
 User Gallery

Registered: 06/18/15
Posts: 22
Last seen: 8 years, 6 months
need help with agar pics
    #21870783 - 06/28/15 09:53 PM (8 years, 6 months ago)

I have done several agar plates and they do not seem like they look right like the ones in RR video can someone please look at these and tell me which plates are good and where a good place may be to take transfers they do not look like they have contam but are growing funny. it is my first successful time and need some help the plates are about 2 weeks old. i have attempted a few tranfers but they do not seem to be going right with seperation of sectors thankyou


Edited by madchemist1 (06/28/15 10:29 PM)


Extras: Filter Print Post Top
InvisibleK1ngSp4de
CHUT UP!!!
I'm a teapot User Gallery


Folding@home Statistics
Registered: 01/16/12
Posts: 1,680
Loc: Flag
Re: need help with agar pics [Re: madchemist1]
    #21870804 - 06/28/15 10:00 PM (8 years, 6 months ago)

That looks like a heavy bacterial contamination, I don't think you'll be getting anything from those.


--------------------
                                    PC Repair and Troubleshooting Forum

If a law is unjust, a man is not only right to disobey it, he is obligated to do so.
                                                - Thomas Jefferson

                    Si peccasse negamus fallimur et nulla est in nobis veritas.


Extras: Filter Print Post Top
Offlinemadchemist1
Stranger
 User Gallery

Registered: 06/18/15
Posts: 22
Last seen: 8 years, 6 months
Re: need help with agar pics [Re: K1ngSp4de]
    #21870841 - 06/28/15 10:08 PM (8 years, 6 months ago)

all of them?


Extras: Filter Print Post Top
Offlinemadchemist1
Stranger
 User Gallery

Registered: 06/18/15
Posts: 22
Last seen: 8 years, 6 months
Re: need help with agar pics [Re: K1ngSp4de]
    #21870851 - 06/28/15 10:09 PM (8 years, 6 months ago)

is it easier or better to do it from the mushroom


Extras: Filter Print Post Top
InvisibleK1ngSp4de
CHUT UP!!!
I'm a teapot User Gallery


Folding@home Statistics
Registered: 01/16/12
Posts: 1,680
Loc: Flag
Re: need help with agar pics [Re: madchemist1]
    #21870941 - 06/28/15 10:21 PM (8 years, 6 months ago)

All I see is one plate in your gallery.

EDIT: You have five pictures of the same plate in your gallery.


--------------------
                                    PC Repair and Troubleshooting Forum

If a law is unjust, a man is not only right to disobey it, he is obligated to do so.
                                                - Thomas Jefferson

                    Si peccasse negamus fallimur et nulla est in nobis veritas.


Edited by K1ngSp4de (06/28/15 10:22 PM)


Extras: Filter Print Post Top
Offlinemadchemist1
Stranger
 User Gallery

Registered: 06/18/15
Posts: 22
Last seen: 8 years, 6 months
Re: need help with agar pics [Re: K1ngSp4de]
    #21870995 - 06/28/15 10:29 PM (8 years, 6 months ago)

i just figured out how to add them to this post up top edited it


Extras: Filter Print Post Top
Offlinemadchemist1
Stranger
 User Gallery

Registered: 06/18/15
Posts: 22
Last seen: 8 years, 6 months
Re: need help with agar pics [Re: K1ngSp4de]
    #21871006 - 06/28/15 10:31 PM (8 years, 6 months ago)

i took two pics of each plate to make sure i got good shots LOL


Extras: Filter Print Post Top
InvisibleK1ngSp4de
CHUT UP!!!
I'm a teapot User Gallery


Folding@home Statistics
Registered: 01/16/12
Posts: 1,680
Loc: Flag
Re: need help with agar pics [Re: madchemist1]
    #21871030 - 06/28/15 10:35 PM (8 years, 6 months ago)

They all look pretty gross to me. Not how they should at all, looks like a bad syringe?


--------------------
                                    PC Repair and Troubleshooting Forum

If a law is unjust, a man is not only right to disobey it, he is obligated to do so.
                                                - Thomas Jefferson

                    Si peccasse negamus fallimur et nulla est in nobis veritas.


Extras: Filter Print Post Top
Offlinemadchemist1
Stranger
 User Gallery

Registered: 06/18/15
Posts: 22
Last seen: 8 years, 6 months
Re: need help with agar pics [Re: K1ngSp4de]
    #21871044 - 06/28/15 10:39 PM (8 years, 6 months ago)

right on i was wondering that i started new ones today can u tell me if it might be easier to start from a mushroom than from the syringe?


Extras: Filter Print Post Top
InvisibleK1ngSp4de
CHUT UP!!!
I'm a teapot User Gallery


Folding@home Statistics
Registered: 01/16/12
Posts: 1,680
Loc: Flag
Re: need help with agar pics [Re: madchemist1]
    #21871220 - 06/28/15 11:20 PM (8 years, 6 months ago)

From a fresh mushie would be good. I will try to start straight from print.

Print>agar>isolate>grain>G2G>mono


Edited by K1ngSp4de (06/30/15 02:42 AM)


Extras: Filter Print Post Top
InvisibleSubnet Mask
 User Gallery


Registered: 05/08/15
Posts: 903
Loc: Flag
Re: need help with agar pics [Re: K1ngSp4de]
    #21871456 - 06/29/15 12:09 AM (8 years, 6 months ago)

it looks like you could take some wedges from 6 and 7.  The rest look like garbage.


Extras: Filter Print Post Top
Invisiblenatedawgnow
Rocky mountain hood rat
 User Gallery


Registered: 02/09/15
Posts: 8,939
Loc: ation
Trusted Cultivator
Re: need help with agar pics [Re: madchemist1]
    #21871470 - 06/29/15 12:14 AM (8 years, 6 months ago)

Every plate is bacterial, man. I dont think you can salvage that. Re try after perfecting your sterile technique.


--------------------


Edited by natedawgnow (06/29/15 12:14 AM)


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: need help with agar pics [Re: natedawgnow]
    #21871567 - 06/29/15 12:51 AM (8 years, 6 months ago)

Quote:

Subnet Mask said:
it looks like you could take some wedges from 6 and 7.  The rest look like garbage.



I wouldn't. Those are very questionable.

Quote:

natedawgnow said:
Every plate is bacterial, man. I dont think you can salvage that. Re try after perfecting your sterile technique.



:whathesaid::whathesaid::whathesaid:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
InvisibleFriedEggS
I'm a teapot


Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
Re: need help with agar pics [Re: natedawgnow]
    #21871576 - 06/29/15 12:54 AM (8 years, 6 months ago)

sorry to laugh but it looks like you got so excited about using agar that you jizzed on every plate during your transfers :lol:



and yea there's bacteria in every plate


--------------------

(Yes, the egg is real)

How to post pics


Extras: Filter Print Post Top
OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
Re: need help with agar pics [Re: FriedEgg]
    #21871688 - 06/29/15 01:31 AM (8 years, 6 months ago)

You might be able to take a transfer from the this one, but try very hard to avoid the bacteria. It probably still won't be clean until a few transfers though.


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: need help with agar pics [Re: Mad Season]
    #21871696 - 06/29/15 01:36 AM (8 years, 6 months ago)

Quote:

Mad Season said:
Quote:

Subnet Mask said:
it looks like you could take some wedges from 6 and 7.  The rest look like garbage.



I wouldn't. Those are very questionable.




That's from 6 and 7. And it's got a glossy look because it's both mycelium and bacteria. Mycelium should be fluffy/rhizo and amazingly aggressive looking. Not like that. I'd seriously just review the techniques and start over. Working with hidden bacteria contaminations is the bane of our existence. I know I'm not the only one who agrees.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
Invisiblenatedawgnow
Rocky mountain hood rat
 User Gallery


Registered: 02/09/15
Posts: 8,939
Loc: ation
Trusted Cultivator
Re: need help with agar pics [Re: Mad Season]
    #21871720 - 06/29/15 01:46 AM (8 years, 6 months ago)

definitely not the only one. I've had bacteria ride out a few transfers under the myc. It can be hard to out run bacteria because it grows real fast


--------------------


Extras: Filter Print Post Top
Offlineshroomyaxn
Male User Gallery


Registered: 11/13/11
Posts: 276
Last seen: 2 years, 2 months
Re: need help with agar pics [Re: natedawgnow]
    #21872656 - 06/29/15 10:03 AM (8 years, 6 months ago)

You could also try inoculating a small pint jar of rye with your MS syringe.  Once fully colonized, take a single grain and place it on agar. 

If you have no spore prints or fruits to clone this method will work. 

I too tried multi-spore -> agar and it is challenging to get it started.  I ended up with many plates contaminated before the spores could germinate. 
You are better off with spore prints, mushroom tissue  or colonized rye grains to get your agar started up.


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: need help with agar pics [Re: shroomyaxn]
    #21872693 - 06/29/15 10:11 AM (8 years, 6 months ago)



All from spore syringes. Not even wrapped in film. My experiences have been that syringes are awesome. Just need a few drops and let it streak across the plate.

I'm not sure why you got beef with syringes.. they're the exact same success as prints. Actually it's probably better than prints. It's pretty easy to slightly lift the lid and only have the needle in the dish, drop a couple drops and tilt the dish around to let it go all around the dish. No gloved hand or syringe even goes near the plate. Just the flamed needle you used.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
OfflineAchillita
Back to the basics
Male


Registered: 05/26/14
Posts: 4,565
Last seen: 3 years, 10 days
Re: need help with agar pics [Re: Mad Season]
    #21872727 - 06/29/15 10:20 AM (8 years, 6 months ago)

Every time I've made a syringe and tested it on agar, it has contaminated. But for some reason I can't make syringes anymore...
But agar is the shit!

I'd suggest trying to fix your sterile technique. Since you're pouring the plates, I'd keep a plate as a control. BUt what's your technique? Are you suing a SAB or a flowhood?


--------------------


Extras: Filter Print Post Top
Offlinemadchemist1
Stranger
 User Gallery

Registered: 06/18/15
Posts: 22
Last seen: 8 years, 6 months
Re: need help with agar pics [Re: Achillita]
    #21875707 - 06/29/15 09:29 PM (8 years, 6 months ago)

flowhood


Extras: Filter Print Post Top
Offlinemadchemist1
Stranger
 User Gallery

Registered: 06/18/15
Posts: 22
Last seen: 8 years, 6 months
Re: need help with agar pics [Re: shroomyaxn]
    #21875715 - 06/29/15 09:31 PM (8 years, 6 months ago)

can u use a brf piece and do the same thing


Extras: Filter Print Post Top
InvisibleCH HELL
Brain Sturgeon
Male User Gallery

Registered: 10/02/08
Posts: 6,610
Loc: mars Flag
Trusted Cultivator
Re: need help with agar pics [Re: madchemist1] * 1
    #21875766 - 06/29/15 09:40 PM (8 years, 6 months ago)

Yes that is bacteria,  you may get growth from them yet.  As soon as you see myc growth transfer to a new plate avoiding any bacteria slime.  The syringe you had was bad or go contaminated before going to agar.


And yes again you can use cakes just be sure the section you grab is fro the center of the cake not the top or bottom.  If you have a clean cake then just pull a small piece of myc from the center/side of the cake should be easy just don't touch anything else.

GL,
CH


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2  [ show all ]

Shop: PhytoExtractum Buy Bali Kratom Powder   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* Agar culture highly contaminated CultyVader 3,093 10 03/25/03 05:51 PM
by CultyVader
* Re: My HORRIBLE accident with agar (pic) Coma of Souls 1,266 2 01/15/00 10:46 AM
by camel
* Re: mold in agar plate Anonymous 12,189 3 12/04/99 12:35 AM
by Anonymous
* golden teacher agar asking fugu 3,052 6 01/31/03 12:59 PM
by debianlinux
* agar/peroxide conc. iudexk 1,325 1 08/27/01 01:17 PM
by jonnyshaggs420
* Why grow on agar? stcore 2,012 1 07/29/02 05:03 PM
by bluhoney
* Wedge of light growth on agar
( 1 2 all )
Parafaragaramus 1,187 27 03/05/18 09:28 PM
by Mr.Kaption
* Benefits of Agar? celsius 9,381 10 12/01/19 12:36 AM
by wildernessjunkie

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,086 topic views. 32 members, 224 guests and 38 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.03 seconds spending 0.007 seconds on 14 queries.