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OfflinefirstTIMER420
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Re: Grain lids:Polyfill vs SHIP/Monotub ?'s [Re: Psilosoulful]
    #21887297 - 07/02/15 10:17 AM (8 years, 6 months ago)

gotcha guys.  Yea about the micropore tape, and having the open hole in the jar, that's why i was asking about the dry verm layer which i was assuming was there because of the open holes to catch and contams coming in the jar.

my grains came in today about 8 days earlier than i was told so im fucking stoked. i have everything except my SFD, so im thinking about just using the tyvek suit i have to do the lids.

But i REALLY want to do the RTV SHIP on my SFD lid for the sake of making it easier to innoc, but at the same time i do see what you are saying about learning the procedures.



And i said, i have rye berries.


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InvisibleJuiceh
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Re: Grain lids:Polyfill vs SHIP/Monotub ?'s [Re: firstTIMER420]
    #21887836 - 07/02/15 01:00 PM (8 years, 6 months ago)

Polyfill is garbage for a filter. People bitch about the pore sizes of tyvek and say it's garbage. But no one ever seems to mention that you won't ever even come close to getting stuffed polyfill down to the pore sizes found with tyvek... The best thing you can use is SFDs. Wait until you get some SFDs for best results. Use 1/4" GE holes. I wouldn't use a quarter to trace SFDs. I've used a penny or a nickel. If you really wanted to you could get away with using a dime to get the most out of your SFD discs.

Rye berries are expensive. Unless you're growing stones, I'd just get whatever is cheapest locally. You've gotta have a feed store or tractor supply company near your area. $15 for 50 lbs of whole oats is hard to beat from TSC.

You can PC qt grain jars on their sides as long as they are on the top level out of the water above the standing jars. I think you should be able to fit 10 total in a 22qt PC, 7 standing, 3 laying on top. That's how many I can fit in my AA921. I PC grains for 90 mins at 20psi, I used to do 90 mins at 15psi with no issues.

If you are going to make RTV SHIPs the best kind to use is the clear kind. You don't need the red high temp stuff, all RTV will hold up past the temperatures we need them for. The clear stuff makes finding the hole through the silicone easy.

I use an inoculation loop to transfer spores to agar. I've read on hear that a blade can damage spores, but I dunno if that really matters much since there will be so many undamaged spore that get scrapped along with them. However, your best bet is to skip making a spore syringe and put the spores to agar first, transfer clean growth to another plate. Then make inoculant from the clean plate, or knock up a jar with a wedge from the clean plate and G2G it out to the other jars once it has colonized cleanly.

Quote:

shroomyaxn said:
The reason I boil my rye berries after a 12-20hr soak is because the grains can easily be steam dried after a 5-10min boil. 
Once I dump the rye berries into the colander I vigorously shake them until the steam no longer comes off the grains.  I then toss them around with a spoon for another 15-20 mins until they are cooled.



Do this. I basically do the same thing. Except I use bucket screens so I can easily collect the grain water for GWLC.


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Edited by Juiceh (07/02/15 01:01 PM)


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InvisibleChilldog
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Re: Grain lids:Polyfill vs SHIP/Monotub ?'s [Re: firstTIMER420]
    #21888007 - 07/02/15 01:46 PM (8 years, 6 months ago)


This is how I prefer grain lids. One hole covered by sfd.

Open lid to inoc. SHIPs are a pain.


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InvisibleJuiceh
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Re: Grain lids:Polyfill vs SHIP/Monotub ?'s [Re: Chilldog]
    #21888185 - 07/02/15 02:27 PM (8 years, 6 months ago)

Your lids are upside down. Yes they are easier to take the tops off when they are upside down. Because the seal around the rim of the jar is imperfect when it's metal to glass. This allows for a possible area for unfiltered GE to take place.


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RogerRabbit said:You need a bigger pressure cooker for this hobby. RR


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InvisibleChilldog
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Re: Grain lids:Polyfill vs SHIP/Monotub ?'s [Re: Juiceh]
    #21888377 - 07/02/15 03:11 PM (8 years, 6 months ago)

Maybe so. If I start to have issues I will consider flipping them. I believe it to be better than plastic lids at least. Them bitches have very little "seal"


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InvisibleJuiceh
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Re: Grain lids:Polyfill vs SHIP/Monotub ?'s [Re: Chilldog]
    #21888421 - 07/02/15 03:20 PM (8 years, 6 months ago)

True. I haven't used plastic lids much for grains. Only once with shiitake, I used the rubber rings and had syringe filters for GE. Bad choice, the syringe filters suck for GE for spawn, but after eventual colonization everything was fine.  I have only been using the plastic lids for LC, with the rubber ring. I have seen people use the plastic lids for grains and then wrap the edge of the lid with parafilm. Seems kinda wasteful of parafilm tho. You can also RTV the rubber ring to the lids, pretty sure I saw that on here once.


Edited by Juiceh (07/02/15 03:24 PM)


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OfflinefirstTIMER420
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Re: Grain lids:Polyfill vs SHIP/Monotub ?'s [Re: Juiceh]
    #21892101 - 07/03/15 10:55 AM (8 years, 6 months ago)

ok guys, I have decided on making a SFD lid, but also adding a SHIP on it, so basically 2 holes one for GE and one for INNOC, for some reason, I don't like the idea of unscrewing the lid to innoc and feel this is going to lead to contams.

Im confused, and trying to understand this, so can someone please explain how the RTV actually re-seals itself after being punctured?

As far as preparing the grains, I rinse them, then soak for 12-20 hrs, but how long do I boil them for? and is it a boil like you do when sterilizing jars for the PF tek or a simmer?  then after the boil I stir around in a colander for a couple minutes until the steam stops then stir around a little while more, How long do I let it sit before I can put in jars and PC it?  and when PCing it, Ive been reading and most people suggest letting the jars sit overnight before taking them out to innoc. 

Im new to using a PC, and my question this time is: i wait for it to depressurize before taking the lid off and taking the jars out and placing them in the SAB to cool further overnight.  What is the safe pressure reading on the gauge to take them out(like what PSI is equal to atmospheric pressure)


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Offlinespacechildo
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Re: Grain lids:Polyfill vs SHIP/Monotub ?'s [Re: firstTIMER420]
    #21892135 - 07/03/15 11:04 AM (8 years, 6 months ago)

Quote:

firstTIMER420 said:
ok guys, I have decided on making a SFD lid, but also adding a SHIP on it, so basically 2 holes one for GE and one for INNOC, for some reason, I don't like the idea of unscrewing the lid to innoc and feel this is going to lead to contams.




how do you think we get agar wedges into grain jars? you think agar work is a contam vector?
how is pushing your newly flamed clean needle through dirty alcohol not gonna be a contam vector?

you're being counter productive man.

for the other q's check out www.mushroomvideos.com it has a rye prep video. I think its in my sig too. turned them off ..

0 psi is safe to open the pc. no reason not to let the pc cool down before taking stuff out either,
unless you need to use the pc again quickly..


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OfflinefirstTIMER420
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Re: Grain lids:Polyfill vs SHIP/Monotub ?'s [Re: spacechildo]
    #21892154 - 07/03/15 11:11 AM (8 years, 6 months ago)

idk what your trying to say about pushing it through alcohol, but im gonna assume that you are talking about wiping off the needle after flaming it with alcohol, which i never do.

Downloaded the videos i was just checking for some other feedback on methods.

also, before inoculating the PC'd jars, should you wipe them down with alcohol?


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Offlinespacechildo
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Re: Grain lids:Polyfill vs SHIP/Monotub ?'s [Re: firstTIMER420]
    #21892182 - 07/03/15 11:18 AM (8 years, 6 months ago)

I'm talking about your SHIP! the injection port. it cant be flamed red hot so you wanna wash it off with iso.

I wipe between the lid and rim before opening them.

you may need to find your own sweet spot regarding the grain prep.
how dry they need to be before PC'ing depends on how much water you have gotten inside them.
a little extra moisture on the outside can be cooked into the grains during the PC run if the grains arent hydrated to 100% capacity.


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Re: Grain lids:Polyfill vs SHIP/Monotub ?'s [Re: spacechildo]
    #21895988 - 07/04/15 08:53 AM (8 years, 6 months ago)

Lid and the rim? like the rim on the side of the jar the lip that comes out? sorry i want to be thourough.

also, my SFD's came in, and i am going to make my lids today.  on your SFD lid tek in your sig, the dude makes a SFD lid with a SHIP.  I am torn, i want to make the ship but at the same time i don't want to make it.  you are saying not to use a SHIP, but going by your logic of contamination, wouldn't unscrewing the lid and opening it introduce more contams than wiping the SHIP with alcohol and then piercing it?


i want to start making the lids around 12 or 1 today, but still want to have more info before i attempt it, as i really really don't want to waste and SFD they aren't cheap(i had to pay 20$ for 12 wide mouth filters...i hate shipping charges.)

So, im excited but definitely do not want to mess up.


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Re: Grain lids:Polyfill vs SHIP/Monotub ?'s [Re: firstTIMER420]
    #21896682 - 07/04/15 01:18 PM (8 years, 6 months ago)

Quote:

firstTIMER420 said:
I am torn, i want to make the ship but at the same time i don't want to make it.  you are saying not to use a SHIP, but going by your logic of contamination, wouldn't unscrewing the lid and opening it introduce more contams than wiping the SHIP with alcohol and then piercing it?




no because you do it in a SAB. the air is still. we all do it when we put agar wedges into grain jars.
your needle can/may/will get dirty from pushing it through a SHIP.
we open agar plates in SABs, do transfers, open lc jars, open grain jars for g2g.


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Re: Grain lids:Polyfill vs SHIP/Monotub ?'s [Re: spacechildo]
    #21896794 - 07/04/15 01:41 PM (8 years, 6 months ago)

i am going to go with the regular SFD lids.  Also going to make a couple SFD jars with some grain so i can spawn some trays and use my SGFC too, doesn't make sense to let them go to waste!


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Re: Grain lids:Polyfill vs SHIP/Monotub ?'s [Re: firstTIMER420]
    #21896838 - 07/04/15 01:55 PM (8 years, 6 months ago)

what other than grains were you gonna use the jars for?


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Re: Grain lids:Polyfill vs SHIP/Monotub ?'s [Re: spacechildo]
    #21896975 - 07/04/15 02:37 PM (8 years, 6 months ago)

thats it for now, obviously they can be used for more things in the future but im not sure yet.  why?

im about to get started, any more advice before I get this going?

just want to double check. 1/4" hole on the SFD jars and a 3/8" hole for the SHIP hole

and im just going to try my first mono grow with the spore syringes I have until I purchase my agar materials, which should be within the next couple of weeks.

space, im going to follow the SFD tek in your sig, but without the SHIP part.  Basically its just cutting a 1/4" hole in the lid and gluing a piece of SFD over it.


Edited by firstTIMER420 (07/04/15 02:47 PM)


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Re: Grain lids:Polyfill vs SHIP/Monotub ?'s [Re: firstTIMER420]
    #21897047 - 07/04/15 02:53 PM (8 years, 6 months ago)

I dont use SHIps but 3/8 sounds like a lot. On my jars that still has ships i think the holes are 1/4" tops. you only need room for the needle.
but if TLs lid tek says 3/8 just do it.

scratch the metal on your lid before applying silicone. makes it stick better.


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Re: Grain lids:Polyfill vs SHIP/Monotub ?'s [Re: spacechildo]
    #21897071 - 07/04/15 02:59 PM (8 years, 6 months ago)

gotcha.

also, the same thing applies I need to put foil over the jar lids before pcing. just making sure.

ill post back with the results of my lids.


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Re: Grain lids:Polyfill vs SHIP/Monotub ?'s [Re: firstTIMER420]
    #21897083 - 07/04/15 03:03 PM (8 years, 6 months ago)

sfd/cfd are different but I'd just do foil either way :shrug:


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Re: Grain lids:Polyfill vs SHIP/Monotub ?'s [Re: spacechildo]
    #21897155 - 07/04/15 03:22 PM (8 years, 6 months ago)

like you said, i think 3/8 hole is pretty big, i would like to think 1/4 would be big enough for GE


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Re: Grain lids:Polyfill vs SHIP/Monotub ?'s [Re: firstTIMER420]
    #21897163 - 07/04/15 03:24 PM (8 years, 6 months ago)

weren't we talking about SHIPs? :stoned:

I'd just blaze one to relax and do everything 1/4".


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