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Offlinenew2doinstuff
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Doc's WBS quart tek
    #21870026 - 06/28/15 07:06 PM (8 years, 7 months ago)

i saw doc's WBS tek, and realized, in the future- that's the starting method... I'll probably use and go directly to bulk, instead of agar.

BUT, question being, obviously these lids he suggests..... are used for innoculating. What type of lid should i use for transfers?

***The agar is gone in the agar dishes, has been gone since last night in most of them... I hope this isn't a problem.**
--Throughout my mini's i have MANY starting grow spots (ring centers??) (i used 1cc per jar, derp... beginner) I'm probably going to put two or three (four) of these areas into each jar.

Second problem is- when i PC'd the lids i do have, they went rusty after letting them cool in the PC overnight. (quick!) ay idea how i can stop this, and what alternative lids i should use for this tek, but for transfers? I looked around and cant find one i think would work, unless i'm missing something


edit: there's about 8 or 10 of these per glad round (screw) so i'mm probably use the biggest ones.. this works out nicely anyways
editnote: is 2 weeks for an agar dish to fully colonize the bottom, too long?


Edited by new2doinstuff (06/28/15 07:08 PM)


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OfflineSyntheticwords
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Re: Doc's WBS quart tek [Re: new2doinstuff]
    #21870056 - 06/28/15 07:12 PM (8 years, 7 months ago)

I use synthetic filter discs for my g2g transfers

Read this http://www.shroomery.org/forums/showflat.php/Number/10900539

It helped me tons


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Offlinenew2doinstuff
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Re: Doc's WBS quart tek [Re: Syntheticwords]
    #21870200 - 06/28/15 07:40 PM (8 years, 7 months ago)

TLDR; i work 12 hours a day, and dont have time before i go to bed plus making the lids. Idc about the 9 yards of this crap... all that i just wanna know how to make the lids. giving me that much information is going to trigger my anxiety to bad i'll quit entirely


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OfflineSyntheticwords
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Re: Doc's WBS quart tek [Re: new2doinstuff]
    #21870262 - 06/28/15 07:51 PM (8 years, 7 months ago)

reading that 9 yards of crap will help you become a better grower

But who has time to be a good grower?
Throw together whatever kind of slop you can get since you have no time


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Offlinenew2doinstuff
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Re: Doc's WBS quart tek [Re: Syntheticwords]
    #21870354 - 06/28/15 08:12 PM (8 years, 7 months ago)

i want information for one thing, and not 900 other things including that thing.

LID TEK FOR TRANSFERS?


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Invisibleuncle_rico
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Re: Doc's WBS quart tek [Re: new2doinstuff]
    #21870543 - 06/28/15 08:56 PM (8 years, 6 months ago)

SFD under a 3/8" hole in a plastic lid.  or EZ Felt or a piece of SFD RTV'd over a 3/8" hole in a plastic lid. (for a G2G lid)

don't be a dick


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InvisibleTheEaglesGift
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Registered: 04/10/11
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Re: Doc's WBS quart tek [Re: uncle_rico]
    #21870698 - 06/28/15 09:32 PM (8 years, 6 months ago)

Quote:

uncle_rico said:
SFD under a 3/8" hole in a plastic lid.  or EZ Felt or a piece of SFD RTV'd over a 3/8" hole in a plastic lid. (for a G2G lid)

don't be a dick



Quote:

don't be a dick



Quote:

don't be a dick
don't be a dick




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Offlinenew2doinstuff
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Re: Doc's WBS quart tek [Re: uncle_rico]
    #21870719 - 06/28/15 09:37 PM (8 years, 6 months ago)

i'm doing a2g but this shouldnt make much difference. I was told I could use dual layered Micropore tape over the hole (outside), and Innoc points (svc silicon) It would allow gas exchange, and  also filter the air of contams. correct? I have metal lids, and a rust problem, (that i guess isnt a problem)

i apologize if i'm being a dick. I already have a shitton of info to work with, and there is a reason I asked a specific question. I dont have a lot of time to research this specifically. My WBS is literally already soaking and i have a pilot hole drilled and my jars (pretty much) ready to be filled, minus realizing Doc's tec is specifically for innoculatied WBS jars, and not for transfers.


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InvisibleTheEaglesGift
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Re: Doc's WBS quart tek [Re: new2doinstuff]
    #21870735 - 06/28/15 09:42 PM (8 years, 6 months ago)

Quote:

I was told I could use dual layered Micropore tape over the hole (outside)



Micropore tape makes a shitty GE filter. I've looked at this shit under a microscope, the holes in it are huge. If tape is all you have, I suggest quadrupling up on it.

Quote:

and Innoc points (svc silicon)




If you're using g2g to inoculate then you don't need silicone ports.

Even if you plan to use a needle for anything, I suggest cracking the lid inside of a Still Air Box and inoculating with your liquid, via needle, right straight into the medium. I think inoculation ports are relatively pointless.

g2g jars only need a gas exchange port, that's it.


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Offlinenew2doinstuff
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Re: Doc's WBS quart tek [Re: TheEaglesGift]
    #21870808 - 06/28/15 10:01 PM (8 years, 6 months ago)

SHIP has seemed to work for me, its just so i can reuse the jars in an easier process later (potentially)

so basically, just the micropore tape will do well in preventing unneeded things, and can do the gas exchange. double layered. Correct?

that's all i really needed to know, i have everything i need for plenty of methods and experimentations, but for the method i am currently doing, this was the info i needed. I have pint, half pint, quart, agar supplies (also prepped jars) etc.... many things.


The next time I do this, I'm going to do SHIP directly to WBS to Bulk.


Edited by new2doinstuff (06/28/15 10:04 PM)


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InvisiblePastywhyteMDiscord
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Re: Doc's WBS quart tek [Re: new2doinstuff]
    #21870824 - 06/28/15 10:05 PM (8 years, 6 months ago)

I have used micropore tape for grain jars. It can work layered up, but its far from ideal and can fail once in a while.


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Offlinenew2doinstuff
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Re: Doc's WBS quart tek [Re: Pastywhyte]
    #21870937 - 06/28/15 10:20 PM (8 years, 6 months ago)

Hmmm. I saw some threads saying TYVEK isn't preferred anymore or something which is what i HAD planned on using.....  If I can use micro-spore tape, and replace the tyvek underneath, (it's free....) another concern i had about that tek, is i see DROVES of people saying DO NOT use rubbing alcohol AFTER flame sterilizing.

if this is not the case, I'll just go buy coffee filters and rubber bands....  I can probably still do transfers with these lids, i guess. If i use alcohol on the rounds, i can hold them upside down and scrape the insides into the jar, and replace the top.... I'm just worried the jars will come apart and arent suited for this, unless that's just me thiking too much


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InvisibleTheEaglesGift
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Re: Doc's WBS quart tek [Re: new2doinstuff]
    #21871140 - 06/28/15 11:03 PM (8 years, 6 months ago)

I suggest buying synthetic or cellulose filter discs for your GE filters, if you want to do it right.

How to do Grain to Grain (G2G) Transfers in a Still-Air Box (SAB) Video


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Offlinenew2doinstuff
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Re: Doc's WBS quart tek [Re: new2doinstuff]
    #21871209 - 06/28/15 11:18 PM (8 years, 6 months ago)

Thanks. My mycellium seem to have gone yellow or brown, when looking through the bottom of the rounds, but are white when looking from the top. The agar is gone. it has been. Could this be a moisture issue, or does it sound contammed? (cant post pics rn)


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Offlinenew2doinstuff
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Re: Doc's WBS quart tek [Re: new2doinstuff]
    #21871224 - 06/28/15 11:21 PM (8 years, 6 months ago)

spending more money on filter discs isnt an option when they seem to be a dollar a piece, and im broke right now anyways. i need a solution for >tomorrow if this isnt contammed (will post pics later)


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InvisibleTheEaglesGift
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Re: Doc's WBS quart tek [Re: new2doinstuff]
    #21871268 - 06/28/15 11:28 PM (8 years, 6 months ago)

What do you mean that the agar is gone?

If you're tight on money and time I suggest taping a square of postal tyvek over a 1/4 inch GE hole with a few larger squares of micropore tape. I used to do 2 layers of tyvek and 2 layers of tape. I had good success with it, though I don't use it anymore.



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Offlinenew2doinstuff
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Re: Doc's WBS quart tek [Re: new2doinstuff]
    #21871276 - 06/28/15 11:30 PM (8 years, 6 months ago)

it like ate it or something


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Offlinenew2doinstuff
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Re: Doc's WBS quart tek [Re: new2doinstuff]
    #21871391 - 06/28/15 11:54 PM (8 years, 6 months ago)



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InvisibleTheEaglesGift
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Re: Doc's WBS quart tek [Re: new2doinstuff]
    #21871431 - 06/29/15 12:05 AM (8 years, 6 months ago)

The agar is still there, it's just colonized by the mycelium.


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Offlinenew2doinstuff
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Re: Doc's WBS quart tek [Re: TheEaglesGift]
    #21871510 - 06/29/15 12:27 AM (8 years, 6 months ago)

Oh wow it coated both sides neat


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Invisibleuncle_rico
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Re: Doc's WBS quart tek [Re: new2doinstuff]
    #21871515 - 06/29/15 12:28 AM (8 years, 6 months ago)

You can cut up a $1 SFD to do 8 to 10 lids (RTV'd over the hole).

In the mean time (for tomorrow) ... do the lids like doc34 instructs: pull some bunched-up polyfil through a 3/8" hole, trim it. Then put a layer of tyvek under the lid.  I used this type of lid when I was starting with quarts of WBS.  It worked great.

A one piece plastic lid with a filter RTV'd over the hole is better for me now.  clean, simple, easy for doing G2G transfers.

don't think too much.

do something, check out how you like it and keep going or adjust.

good luck


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InvisibleTheEaglesGift
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Re: Doc's WBS quart tek [Re: uncle_rico]
    #21871525 - 06/29/15 12:34 AM (8 years, 6 months ago)

From the 3rd picture, the bottom doesn't look colonized to me. I've never seen mycelium actually colonize the bottom of a agar plate/dish.

Op, if you decide to stuff poly through a GE hole, make sure the poly is TIGHT.


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Offlinenew2doinstuff
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Re: Doc's WBS quart tek [Re: TheEaglesGift]
    #21873850 - 06/29/15 02:24 PM (8 years, 6 months ago)

Thanks. I read something about being able to push a needle through it with resistance, but not HARD


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Offlinenew2doinstuff
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Re: Doc's WBS quart tek [Re: new2doinstuff]
    #21873852 - 06/29/15 02:25 PM (8 years, 6 months ago)

btw- currently whippin the wrist over the WBS to DEVO... guess which song.


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InvisibleTheEaglesGift
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Re: Doc's WBS quart tek [Re: new2doinstuff]
    #21874138 - 06/29/15 03:43 PM (8 years, 6 months ago)

I wouldn't push a needle through a polyfil filter, I'd just crack the lid while in a still air box, and inoculate into the open space.


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Invisibleuncle_rico
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Re: Doc's WBS quart tek [Re: new2doinstuff]
    #21874816 - 06/29/15 06:23 PM (8 years, 6 months ago)

Quote:

new2doinstuff said:
btw- currently whippin the wrist over the WBS to DEVO... guess which song.




"Crack that whip!"


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OfflineGalba Cubensis
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Re: Doc's WBS quart tek [Re: new2doinstuff]
    #21875052 - 06/29/15 07:09 PM (8 years, 6 months ago)

Quote:

new2doinstuff said:
i want information for one thing, and not 900 other things including that thing.

LID TEK FOR TRANSFERS?





More Galbas?:cool:


--------------------
RETARDS!  I'LL IGNORE ALL OF YOUR ADVICE!  BUT TELL ME WHAT TO DO STILL!


Edited by Galba Cubensis (06/29/15 07:09 PM)


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