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Invisibleuncle_rico
my own worst enemy
Male User Gallery

Registered: 03/28/06
Posts: 2,664
Re: Doc's WBS quart tek [Re: new2doinstuff]
    #21871515 - 06/29/15 12:28 AM (8 years, 6 months ago)

You can cut up a $1 SFD to do 8 to 10 lids (RTV'd over the hole).

In the mean time (for tomorrow) ... do the lids like doc34 instructs: pull some bunched-up polyfil through a 3/8" hole, trim it. Then put a layer of tyvek under the lid.  I used this type of lid when I was starting with quarts of WBS.  It worked great.

A one piece plastic lid with a filter RTV'd over the hole is better for me now.  clean, simple, easy for doing G2G transfers.

don't think too much.

do something, check out how you like it and keep going or adjust.

good luck


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: Doc's WBS quart tek [Re: uncle_rico]
    #21871525 - 06/29/15 12:34 AM (8 years, 6 months ago)

From the 3rd picture, the bottom doesn't look colonized to me. I've never seen mycelium actually colonize the bottom of a agar plate/dish.

Op, if you decide to stuff poly through a GE hole, make sure the poly is TIGHT.


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Offlinenew2doinstuff
Traveller
Male

Registered: 06/15/14
Posts: 264
Last seen: 5 years, 9 months
Re: Doc's WBS quart tek [Re: TheEaglesGift]
    #21873850 - 06/29/15 02:24 PM (8 years, 6 months ago)

Thanks. I read something about being able to push a needle through it with resistance, but not HARD


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Offlinenew2doinstuff
Traveller
Male

Registered: 06/15/14
Posts: 264
Last seen: 5 years, 9 months
Re: Doc's WBS quart tek [Re: new2doinstuff]
    #21873852 - 06/29/15 02:25 PM (8 years, 6 months ago)

btw- currently whippin the wrist over the WBS to DEVO... guess which song.


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: Doc's WBS quart tek [Re: new2doinstuff]
    #21874138 - 06/29/15 03:43 PM (8 years, 6 months ago)

I wouldn't push a needle through a polyfil filter, I'd just crack the lid while in a still air box, and inoculate into the open space.


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Invisibleuncle_rico
my own worst enemy
Male User Gallery

Registered: 03/28/06
Posts: 2,664
Re: Doc's WBS quart tek [Re: new2doinstuff]
    #21874816 - 06/29/15 06:23 PM (8 years, 6 months ago)

Quote:

new2doinstuff said:
btw- currently whippin the wrist over the WBS to DEVO... guess which song.




"Crack that whip!"


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OfflineGalba Cubensis
azur's handdoll
Male User Gallery


Registered: 06/16/15
Posts: 173
Loc: Norway Flag
Last seen: 8 years, 5 months
Re: Doc's WBS quart tek [Re: new2doinstuff]
    #21875052 - 06/29/15 07:09 PM (8 years, 6 months ago)

Quote:

new2doinstuff said:
i want information for one thing, and not 900 other things including that thing.

LID TEK FOR TRANSFERS?





More Galbas?:cool:


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RETARDS!  I'LL IGNORE ALL OF YOUR ADVICE!  BUT TELL ME WHAT TO DO STILL!


Edited by Galba Cubensis (06/29/15 07:09 PM)


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