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mech
Stranger
Registered: 10/20/13
Posts: 15
Last seen: 8 years, 6 months
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Transfer from contam'ed jar
#21865802 - 06/27/15 07:34 PM (8 years, 7 months ago) |
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I have some PF jars that contaminated after mycelium was well on its way. Can I take a core from the healthy looking part and transfer to agar to try and save it?
I know agar is the way to clean up a culture, but I'm starting agar for the first time. Most of my plates will be MS inoculations but I was wondering about this because I have some nice rhismorphic growth that I'd really like to save.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Transfer from contam'ed jar [Re: mech]
#21865813 - 06/27/15 07:37 PM (8 years, 7 months ago) |
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You can transfer it to agar to save it. If it has contamination on agar cut away from it
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
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Re: Transfer from contam'ed jar [Re: mech]
#21865845 - 06/27/15 07:42 PM (8 years, 7 months ago) |
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Quote:
mech said: I have some PF jars that contaminated after mycelium was well on its way. Can I take a core from the healthy looking part and transfer to agar to try and save it?
I know agar is the way to clean up a culture, but I'm starting agar for the first time. Most of my plates will be MS inoculations but I was wondering about this because I have some nice rhismorphic growth that I'd really like to save.
You could try, but I wouldnt. I wouldnt even open the jar.. you would have to open it up in your still air box and try to grab a piece of myc from under the dirty verm layer. Seems messy 
Better to start from spore. Rhizo doesnt always equal good you will learn.
Some of my best cultures come from the fuzziest plates! Rhizo/fluffy growth usually depends on how many nutrients are in your media. When you look for growth, dont look so much for rhizo, just look for fast, agressive growth that is (most importantly) clean and away from contams on the plate
Good luck!
--------------------
 Amateur Mycologists United AMU Q&A
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Transfer from contam'ed jar [Re: mushpunx]
#21865876 - 06/27/15 07:48 PM (8 years, 7 months ago) |
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Quote:
mushpunx said: You could try, but I wouldnt. I wouldnt even open the jar.. you would have to open it up in your still air box and try to grab a piece of myc from under the dirty verm layer. Seems messy 
agar can work for wild prints. I'm sure it'll work for this if you're cautious, clean and like you said in a sab
Better to start from spore. Rhizo doesnt always equal good you will learn.
  
Some of my best cultures come from the fuzziest plates! Rhizo/fluffy growth usually depends on how many nutrients are in your media. When you look for growth, dont look so much for rhizo, just look for fast, agressive growth that is (most importantly) clean and away from contams on the plate
  
Good luck!
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mech
Stranger
Registered: 10/20/13
Posts: 15
Last seen: 8 years, 6 months
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Re: Transfer from contam'ed jar [Re: Mad Season]
#21865904 - 06/27/15 07:52 PM (8 years, 7 months ago) |
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Thanks for confirming. Just very uncertain about all the non-basic stuff.
Not sure about the procedure now. If I open and pry out the cake inside my SAB, I'm going to spread the contamination everywhere. So I guess I should open the jar outside and pop the cake out. But then, should I bring it inside the SAB and then cut a sample? Or should I cut it out outside, bring it in and then what? How do I minimize the chances that I add more contamination to the hopefully good chunk?
Edit: other replies came as I was typing. I think I'll try it anyway. Agar is cheap. At worst I learn something and practice my technique.
Edited by mech (06/27/15 07:55 PM)
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Transfer from contam'ed jar [Re: mech]
#21865933 - 06/27/15 07:59 PM (8 years, 7 months ago) |
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First off what contamination is it? If it's trich just don't bother. It eats and goes through mycelium. It'll be a part of it and will follow through even if it is white. Otherwise just get a clean chunk outside and take that sample into an sab. Then open it up and make multiple plates with clean samples inside. Then pray.
Also seriously recommend going onto an antibacterial plate. Not just regular agar. If it's too much just go to spores. Ms rarely lets me down
Edited by Mad Season (06/27/15 07:59 PM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Transfer from contam'ed jar [Re: Mad Season]
#21865978 - 06/27/15 08:10 PM (8 years, 7 months ago) |
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Do spores and fuck with the cake
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mech
Stranger
Registered: 10/20/13
Posts: 15
Last seen: 8 years, 6 months
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Re: Transfer from contam'ed jar [Re: Mad Season]
#21865985 - 06/27/15 08:12 PM (8 years, 7 months ago) |
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Thinking now it is trich.
I've seen lots of trich before and it doesn't look like that I'm used to. But I just looked up a guide to contaminants and it does indeed look like the greyer examples.
Yeah, I'm definitely doing spores. Just wondered if I should try this as well. Leaning towards "no!" now.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Transfer from contam'ed jar [Re: mech]
#21866003 - 06/27/15 08:15 PM (8 years, 7 months ago) |
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Transfer from contam'ed jar [Re: bodhisatta]
#21866015 - 06/27/15 08:18 PM (8 years, 7 months ago) |
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That's a beautiful pic. Now I'm saving this thread to my favorites lol
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: Transfer from contam'ed jar [Re: mech]
#21866303 - 06/27/15 09:22 PM (8 years, 7 months ago) |
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You could...FOTTSE
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