Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Kraken Kratom Red Vein Kratom   PhytoExtractum Kratom Powder for Sale   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Bridgetown Botanicals Bridgetown Botanicals   North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Capsules

Jump to first unread post Pages: 1 | 2 | 3 | 4  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 11 hours
Trusted Cultivator
Photo of bacteria on plates
    #21865514 - 06/27/15 06:45 PM (8 years, 7 months ago)




2 of the plates were from a sloppy pour I guess, never poured a bad plate before :shrug:

Anyways a lot of folks ask me what bacteria look like on agar. So I figured I start a thread where people can put up photos of bacteria in plates and jars, mold colonies etc

What to look for

:thumbup:


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: Photo of bacteria on plates [Re: mushpunx]
    #21865532 - 06/27/15 06:48 PM (8 years, 7 months ago)

:aaaargh:


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Photo of bacteria on plates [Re: Buck513]
    #21865881 - 06/27/15 07:48 PM (8 years, 7 months ago)


Penicllin sp. I believe.


Left cocci bacteria
Right aspergillus


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Photo of bacteria on plates [Re: bodhisatta]
    #21866334 - 06/27/15 09:29 PM (8 years, 7 months ago)

Guess how many different species of bacteria is on this plate:

Tissue sample from clone gone wrong :rofl: and that folks is why I make multiple dishes when cloning.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 19 hours
Trusted Cultivator
Re: Photo of bacteria on plates [Re: Mad Season]
    #21866367 - 06/27/15 09:39 PM (8 years, 7 months ago)

That my friend is why we take tissue samples from inside the fruit, as to not grab anything that a contam has landed on....lol.


Extras: Filter Print Post Top
Invisibled0urd3n
Just call me "D"

Registered: 09/15/10
Posts: 5,237
Re: Photo of bacteria on plates [Re: PussyFart]
    #21866399 - 06/27/15 09:46 PM (8 years, 7 months ago)

Bacteria + some purple mold



--------------------


Extras: Filter Print Post Top
OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 19 hours
Trusted Cultivator
Re: Photo of bacteria on plates [Re: d0urd3n]
    #21866418 - 06/27/15 09:50 PM (8 years, 7 months ago)

         


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Photo of bacteria on plates [Re: PussyFart]
    #21866719 - 06/27/15 11:39 PM (8 years, 7 months ago)

Haha PF I do that. I just messed up somehow :P a different plate from the same stem went swimmingly though. It was a small ass stem. Even the base was a cm tops.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
InvisibleFriedEggS
I'm a teapot


Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
Re: Photo of bacteria on plates [Re: Mad Season]
    #21866888 - 06/28/15 12:53 AM (8 years, 7 months ago)

good idea for a thread.


here's my contribution:


--------------------

(Yes, the egg is real)

How to post pics


Extras: Filter Print Post Top
Offlinehamloaf
Loaf of Fam.
Male User Gallery

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
Last seen: 1 year, 8 months
Trusted Cultivator
Re: Photo of bacteria on plates [Re: mushpunx]
    #21867214 - 06/28/15 04:24 AM (8 years, 7 months ago)

:deathstar:


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery

Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: Photo of bacteria on plates [Re: hamloaf]
    #21867230 - 06/28/15 04:46 AM (8 years, 7 months ago)



--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


Extras: Filter Print Post Top
OfflineGreenRabbit
Plutonium Pollinator
Male


Registered: 04/28/13
Posts: 2,667
Loc: In a forest
Last seen: 2 years, 3 months
Re: Photo of bacteria on plates [Re: bodhisatta]
    #21867819 - 06/28/15 09:55 AM (8 years, 7 months ago)

Quote:

bodhisatta said:

Left cocci bacteria
Right aspergillus




That aspergillus looks just like trich to me. How can you tell the difference?


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Photo of bacteria on plates [Re: GreenRabbit]
    #21867833 - 06/28/15 09:59 AM (8 years, 7 months ago)

Trich never looks like that on plates in my experience. Also rarely does it turn green on a plate if it's growing with mycelium too.


Edited by Trusted cuItivator (06/28/15 09:59 AM)


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Photo of bacteria on plates [Re: GreenRabbit]
    #21867837 - 06/28/15 10:00 AM (8 years, 7 months ago)

Trich usually has a crystaline texture to the surface and a bubbling growth pattern. Also like bodhi said it usually needs some decent FAE to sporulate.


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Photo of bacteria on plates [Re: GreenRabbit]
    #21867852 - 06/28/15 10:05 AM (8 years, 7 months ago)

On substrates though powder green look can indeed be a multitude of species and even a few different families. Aspergillus, penicillium, trichoderma.. They all can range in different shades too. The online mushroom community generally calls them all trich. Realistically speaking a microscope would for sure tell what it is.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
Offlinehamloaf
Loaf of Fam.
Male User Gallery

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
Last seen: 1 year, 8 months
Trusted Cultivator
Re: Photo of bacteria on plates [Re: Mad Season]
    #21867875 - 06/28/15 10:12 AM (8 years, 7 months ago)

All three of the aforementioned contam species have their own shade of green.  Been blessed to not run into too much Aspergillus, but have seen a fair share of penicillum and trichoderma.  Penicillum is a teal green, where trichoderma is a dark, deep, forest green color.  Very distinct.  Trichoderma is the bane if the mushroom culture.  HATE!

:deathstar:


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


Extras: Filter Print Post Top
OfflineGreenRabbit
Plutonium Pollinator
Male


Registered: 04/28/13
Posts: 2,667
Loc: In a forest
Last seen: 2 years, 3 months
Re: Photo of bacteria on plates [Re: bodhisatta]
    #21867887 - 06/28/15 10:16 AM (8 years, 7 months ago)

Quote:

bodhisatta said:
Trich never looks like that on plates in my experience. Also rarely does it turn green on a plate if it's growing with mycelium too.




Sweet, good to know. Just started agar so I've only seen trich in PF jars. Pretty sure it was trich everytime


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Photo of bacteria on plates [Re: hamloaf]
    #21867900 - 06/28/15 10:21 AM (8 years, 7 months ago)

Interesting. I've also seen a bright green/mossy green look in certain species of trich. However I know what you mean by the teal look in penicillium. I've seen that quite a bit too.

So looking at bods pic, I can see it's a very fine powdery look. Almost looks like a smooth surface, whereas trich has more visible particle/granite look. Is this a key distinction between the 2 on substrates? Also another distinction as mentioned before is that it doesn't sporulate on plates well. Just curious how you guys visually tell the difference. *runs off to look at pics of them*


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (06/28/15 10:23 AM)


Extras: Filter Print Post Top
Offlinehamloaf
Loaf of Fam.
Male User Gallery

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
Last seen: 1 year, 8 months
Trusted Cultivator
Re: Photo of bacteria on plates [Re: Mad Season]
    #21867932 - 06/28/15 10:33 AM (8 years, 7 months ago)

I don't know what the guys are talking about over the issue of trich not sporulating on agar very often, because I'v had trich break out on several plates over the last 6 years.  Recently had a contam breakout in the lab.  40 plus pre-poured plates that were sitting for over a month and a half ALL had trich in them.  Don't know how long said trich was growing on the plates for.  What is known is as soon as the contam was discovered it was immediately discarded.  Black mold is another that'll sporulate all over your agar media.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Photo of bacteria on plates [Re: hamloaf]
    #21868200 - 06/28/15 11:54 AM (8 years, 7 months ago)

Quote:

hamloaf said:
All three of the aforementioned contam species have their own shade of green.  Been blessed to not run into too much Aspergillus, but have seen a fair share of penicillum and trichoderma.  Penicillum is a teal green, where trichoderma is a dark, deep, forest green color.  Very distinct.  Trichoderma is the bane if the mushroom culture.  HATE!

:deathstar:



Actually different species of each have a multitude of colors.


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 11 hours
Trusted Cultivator
Re: Photo of bacteria on plates [Re: bodhisatta]
    #21868382 - 06/28/15 12:33 PM (8 years, 7 months ago)

A few months ago I was running into what I think was Aspergillus, it is a nasty one too. A little darker green than trich and it seemed to pretty much start off on the very surface as little green speckles.. instead of white like trich. It would turn myc yellow brown wherever it touched it.
Not as nasty as trich but certainly ruined a few tubs.
It still turns up every couple grows too


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
OfflineGreen Bastard
Stranger
Male User Gallery
Registered: 02/26/14
Posts: 199
Loc: Canada Flag
Last seen: 3 years, 8 months
Re: Photo of bacteria on plates [Re: bodhisatta]
    #21868658 - 06/28/15 01:45 PM (8 years, 7 months ago)

Quote:

bodhisatta said:

Penicllin sp. I believe.


Left cocci bacteria
Right aspergillus




A question, Bodhista. The plate on the right... Is the mycelium also contaminated?
I have a few plates, on their 3rd and 4th transfers away from clones, with no glaringly obvious contams, with big differentials like that...


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Photo of bacteria on plates [Re: Green Bastard]
    #21868659 - 06/28/15 01:46 PM (8 years, 7 months ago)

No


Extras: Filter Print Post Top
OfflineGreen Bastard
Stranger
Male User Gallery
Registered: 02/26/14
Posts: 199
Loc: Canada Flag
Last seen: 3 years, 8 months
Re: Photo of bacteria on plates [Re: bodhisatta]
    #21868689 - 06/28/15 01:52 PM (8 years, 7 months ago)

Thank you for the fast, and reassuring reply! Just aggressive growth, eh? Something to keep transferring from then.... :smile:


Extras: Filter Print Post Top
OfflineDhearic
Male User Gallery

Registered: 01/24/14
Posts: 846
Loc: Neverland
Last seen: 9 months, 4 days
Re: Photo of bacteria on plates [Re: Green Bastard]
    #21874192 - 06/29/15 03:59 PM (8 years, 6 months ago)







:whippedcreamhead:


--------------------


Credit where credit is due.


Extras: Filter Print Post Top
Offlinehamloaf
Loaf of Fam.
Male User Gallery

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
Last seen: 1 year, 8 months
Trusted Cultivator
Re: Photo of bacteria on plates [Re: Dhearic]
    #21874208 - 06/29/15 04:04 PM (8 years, 6 months ago)

DEATHSTAR MANIA!  :deathstar:


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Photo of bacteria on plates [Re: hamloaf]
    #21874227 - 06/29/15 04:10 PM (8 years, 6 months ago)

got a plate of clostridium today fucking smelled like feta cheese in the most unpleasant way.


Extras: Filter Print Post Top
InvisibleFriedEggS
I'm a teapot


Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
Re: Photo of bacteria on plates [Re: Dhearic]
    #21874312 - 06/29/15 04:36 PM (8 years, 6 months ago)

Quote:

Dhearic said:





that top left section kinda looks like the head of a baby when viewed through an ultrasound


--------------------

(Yes, the egg is real)

How to post pics


Extras: Filter Print Post Top
Invisiblejbaby007
Badass
I'm a teapot


Registered: 02/28/15
Posts: 1,026
Re: Photo of bacteria on plates [Re: FriedEgg]
    #21874386 - 06/29/15 04:55 PM (8 years, 6 months ago)

I should have saved my plate from earlier today and taken a pic. It had a nasty chunk of bacteria on it. Thank god I've gotten into agar. It's so nice knowing that I can just transfer healthy mycelium away onto another plate and not worry about it.


--------------------


Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,855
Last seen: 17 hours, 50 minutes
Trusted Cultivator
Re: Photo of bacteria on plates [Re: jbaby007]
    #21874543 - 06/29/15 05:27 PM (8 years, 6 months ago)


Sneaky bacteria


Not so sneaky bacteria


--------------------


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Photo of bacteria on plates [Re: Kizzle]
    #21874565 - 06/29/15 05:31 PM (8 years, 6 months ago)

Super sneaky.. where the hell is it kizzle lol. How do you know it has bacteria?

Is it the off center circle taking up almost all of the dish?


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (06/29/15 05:33 PM)


Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,855
Last seen: 17 hours, 50 minutes
Trusted Cultivator
Re: Photo of bacteria on plates [Re: Mad Season]
    #21874650 - 06/29/15 05:49 PM (8 years, 6 months ago)

It's the very light film covering the "uncolonized" area. I don't know how many dishes like that slipped by before I finally noticed it but I started to wonder why the mycelium was suddenly stalling instead of colonizing the whole plate :lol:


--------------------


Extras: Filter Print Post Top
Offlinebburnett227
???
Male
Registered: 04/29/15
Posts: 71
Last seen: 2 years, 11 months
Re: Photo of bacteria on plates [Re: Kizzle]
    #21908979 - 07/07/15 10:00 AM (8 years, 6 months ago)

When ur transferring out from the contaminated plate to a new one, do u cut all of the good myc out for the transfer? Or just a wedge. I'm under the impression the goal here is to have a plate of full myc, so I would think you'd cut all of it....haven't dine agar yet, 2 or 3 more days til clones are  ready, and just wanna be sure I'm at least starting it right.


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 11 hours
Trusted Cultivator
Re: Photo of bacteria on plates [Re: bburnett227]
    #21913513 - 07/08/15 05:22 AM (8 years, 6 months ago)

Quote:

bburnett227 said:
When ur transferring out from the contaminated plate to a new one, do u cut all of the good myc out for the transfer? Or just a wedge. I'm under the impression the goal here is to have a plate of full myc, so I would think you'd cut all of it....haven't dine agar yet, 2 or 3 more days til clones are  ready, and just wanna be sure I'm at least starting it right.





Just a timy wedge dude. Like a grain of rice. This way you can cut away from contams, isolate genetics etc..

It will grow out on the new plat


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
Offlinebburnett227
???
Male
Registered: 04/29/15
Posts: 71
Last seen: 2 years, 11 months
Re: Photo of bacteria on plates [Re: mushpunx]
    #21913574 - 07/08/15 06:04 AM (8 years, 6 months ago)

thank you sir


--------------------
Abe


Extras: Filter Print Post Top
OfflineWill7401
 User Gallery

Registered: 01/27/16
Posts: 154
Last seen: 13 days, 8 hours
Re: Photo of bacteria on plates [Re: FriedEgg]
    #23134139 - 04/19/16 01:16 PM (7 years, 9 months ago)

What is this? I am getting this from time to time but I am really noticing it with the two Cam MS I purchased.


--------------------
Love and Light to us all. Namaste


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Photo of bacteria on plates [Re: Will7401]
    #23134152 - 04/19/16 01:23 PM (7 years, 9 months ago)

That is bacteria.


Extras: Filter Print Post Top
InvisibleLocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
 User Gallery


Registered: 04/17/15
Posts: 7,076
Loc: to the brain Flag
Re: Photo of bacteria on plates [Re: Pastywhyte]
    #23134160 - 04/19/16 01:25 PM (7 years, 9 months ago)

Quote:

Pastywhyte said:
That is bacteria.



Af


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


Extras: Filter Print Post Top
Offlinegeorgeballer
wooms
Male


Registered: 08/28/12
Posts: 211
Last seen: 3 years, 9 months
Re: Photo of bacteria on plates [Re: Will7401]
    #24115720 - 02/24/17 09:50 AM (6 years, 10 months ago)

Quote:

Will7401 said:
What is this? I am getting this from time to time but I am really noticing it with the two Cam MS I purchased.




Is that green a good color for spotting bacteria?


Extras: Filter Print Post Top
OfflineDyn-A-Mite
Awakened


Registered: 02/11/17
Posts: 218
Last seen: 5 years, 4 months
Re: Photo of bacteria on plates [Re: Kizzle]
    #24115767 - 02/24/17 10:05 AM (6 years, 10 months ago)

Quote:

Kizzle said:

Sneaky bacteria


Not so sneaky bacteria




Kizzle that is a perfect example of how bacteria can hitch a ride on seamlessly healthy looking grain. Thanks for providing such an informative picture.


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery

Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 11 hours
Trusted Cultivator
Re: Photo of bacteria on plates [Re: Dyn-A-Mite]
    #24115775 - 02/24/17 10:09 AM (6 years, 10 months ago)

Kizzle's photo of the sneaky bacteria saved my ass on more than a few occasions since I saw his example


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
InvisibleLocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
 User Gallery


Registered: 04/17/15
Posts: 7,076
Loc: to the brain Flag
Re: Photo of bacteria on plates [Re: mushpunx]
    #24119570 - 02/25/17 07:31 PM (6 years, 10 months ago)

Fairly certain these are all bacteria, except the couple obvious molds on that multi plate...


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


Extras: Filter Print Post Top
OfflineQuetzal7
Stranger
Registered: 04/28/17
Posts: 10
Last seen: 7 months, 26 days
Re: Photo of bacteria on plates [Re: LocN9ne]
    #24318591 - 05/14/17 10:26 AM (6 years, 8 months ago)

Quote:

LocN9ne said:
Fairly certain these are all bacteria, except the couple obvious molds on that multi plate...





Very interesting topic !
But as a new grower, someone can explain a bit what's happening with these jars and culture? The grain jars seems nice to me!! what should i notice???
thanks :smile:


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery

Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 11 hours
Trusted Cultivator
Re: Photo of bacteria on plates [Re: Quetzal7]
    #24319239 - 05/14/17 04:57 PM (6 years, 8 months ago)



Maybe this will help a little more, if you look at the quart jar here, if you notice you'll see its got that thick, creamy cottage cheese type look that comes from bacterial infection. Oddly enough this jar did not smell off at all.

I only have this one picture of clean spawn (gallon jars) to compare to right now, and its still not the best photo that was my first go with gallons and the GE hole in the lid was too small.
But you can see the difference yes?


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
Invisibleeatyualive
Eat's You Alive :)
 User Gallery


Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
Trusted Cultivator
Re: Photo of bacteria on plates [Re: mushpunx]
    #24319477 - 05/14/17 06:36 PM (6 years, 8 months ago)

i can't see any of the images in the op. fyi.


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery

Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 11 hours
Trusted Cultivator
Re: Photo of bacteria on plates [Re: eatyualive]
    #24319631 - 05/14/17 07:30 PM (6 years, 8 months ago)

Quote:

eatyualive said:
i can't see any of the images in the op. fyi.




Yea I know, this thread is a couple years old I still don't have a supporter account yet (I know) so I probably deleted them some time in the last couple years to make room for more pics
My fault


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
InvisibleLocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
 User Gallery


Registered: 04/17/15
Posts: 7,076
Loc: to the brain Flag
Re: Photo of bacteria on plates [Re: Quetzal7]
    #24319972 - 05/14/17 10:23 PM (6 years, 8 months ago)

Quote:

Quetzal7 said:
Quote:

LocN9ne said:
Fairly certain these are all bacteria, except the couple obvious molds on that multi plate...





Very interesting topic !
But as a new grower, someone can explain a bit what's happening with these jars and culture? The grain jars seems nice to me!! what should i notice???
thanks :smile:



Also in those first two jars the spikes of mycelium spread throughout are a sign of bacteria... Myc can look rhizomorphic but it will still remain individual strands when you look at it closely... Those right there are all fused into single chunks... The plates that have those random tufts of myc that are raised up from the rest are also signs of bacteria as far as I can figure.


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


Extras: Filter Print Post Top
Invisiblevan hattonFacebookDiscord
Still a noob
 User Gallery


Registered: 11/23/14
Posts: 5,617
Loc: Michigan
Trusted Cultivator
Re: Photo of bacteria on plates [Re: LocN9ne]
    #24322026 - 05/15/17 06:12 PM (6 years, 8 months ago)

:takingnotes: good stuff.


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


Extras: Filter Print Post Top
OfflineTheFlyingEStorm
Novice
Male


Registered: 04/21/17
Posts: 13
Loc: Watching the Clouds,TX Flag
Last seen: 6 years, 4 months
Re: Photo of bacteria on plates [Re: mushpunx]
    #24322063 - 05/15/17 06:28 PM (6 years, 8 months ago)

Stuff like this really helps out new cultivators like myself who don't have enough experience to spot contams yet, thanks for the posts!!


--------------------
Somewhere where the grass is green and the girls are pretty


Extras: Filter Print Post Top
OfflinePrimal Call
Earth Mage
I'm a teapot


Registered: 09/05/10
Posts: 2,766
Loc: Here until here
Last seen: 4 years, 3 months
Re: Photo of bacteria on plates [Re: TheFlyingEStorm]
    #24625217 - 09/13/17 03:10 AM (6 years, 4 months ago)

marking for reference. Great collection of images/info here.

Just in case others find this thread but not this thread.

:bump2:


--------------------
New Cultivator's Guide
Time to fruit? Pinning Strategy and Troubleshooting
My Trade Thread (Fungus, Plants, Herbal Medicine)


:peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace:


Extras: Filter Print Post Top
Offlineorchitis01
Stranger

Registered: 03/27/14
Posts: 214
Last seen: 1 month, 9 days
Re: Photo of bacteria on plates [Re: LocN9ne]
    #24902414 - 01/10/18 06:24 AM (6 years, 20 days ago)

Quote:

LocN9ne said:
Fairly certain these are all bacteria, except the couple obvious molds on that multi plate...





noob question: what's the give away on the jars?


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 11 hours
Trusted Cultivator
Re: Photo of bacteria on plates [Re: orchitis01] * 1
    #24902466 - 01/10/18 07:21 AM (6 years, 20 days ago)

See how thick and white the myc looks? Sometimes it even gets a cottage cheese look. It will look solid like a block sometimes too.
This photo below is another good example


This is the only photo of clean spawn I have at the sec but you can see the difference.



--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
InvisibleLocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
 User Gallery


Registered: 04/17/15
Posts: 7,076
Loc: to the brain Flag
Re: Photo of bacteria on plates [Re: mushpunx]
    #24904644 - 01/11/18 12:36 AM (6 years, 19 days ago)

Quote:

mushpunx said:
See how thick and white the myc looks? Sometimes it even gets a cottage cheese look. It will look solid like a block sometimes too.
This photo below is another good example


This is the only photo of clean spawn I have at the sec but you can see the difference.





:whathesaid:
Those thick white spikes of mycelium are a give away... In clean spawn the myc will be a bunch of individual stands when you look close... Those look like they have hair gel on them...


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


Extras: Filter Print Post Top
InvisibleZiran
The Hero of Time
I'm a teapot User Gallery


Folding@home Statistics
Registered: 02/03/16
Posts: 6,030
Loc: Temple of Time
Re: Photo of bacteria on plates [Re: LocN9ne]
    #24904690 - 01/11/18 01:44 AM (6 years, 19 days ago)

Some molds and bacterias. along with some cube mycelium


--------------------
Song Of Healing
:super: Updated Pf Tek Guide :super:
Ziran's Teks
AMU Q&A Thread
The Chinese word for nature is zìrán and it means that of which is of itself.



Extras: Filter Print Post Top
OfflineDavieDonna
Stranger


Registered: 11/07/17
Posts: 18
Loc: Shattered Heavens
Last seen: 5 years, 11 months
Re: Photo of bacteria on plates [Re: Ziran]
    #24904715 - 01/11/18 02:12 AM (6 years, 19 days ago)

Quote:

Ziran said:
Some molds and bacterias. along with some cube mycelium





Whoa there's a lot going on here


Extras: Filter Print Post Top
Offlinecatnip40
xฬ็
Male User Gallery


Registered: 03/09/12
Posts: 703
Last seen: 8 days, 15 hours
Re: Photo of bacteria on plates [Re: DavieDonna]
    #24904807 - 01/11/18 05:21 AM (6 years, 19 days ago)

:takingnotes::popcorn:


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 11 hours
Trusted Cultivator
Re: Photo of bacteria on plates [Re: catnip40]
    #24904874 - 01/11/18 06:41 AM (6 years, 19 days ago)

I lost my photos in the OP :facepalm: accidentally deleted then



Here's a good one though, a culture thats been contaminated


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
OfflineGlobeLamp
Stranger


Registered: 11/13/17
Posts: 71
Last seen: 5 years, 10 months
Re: Photo of bacteria on plates [Re: mushpunx]
    #24904904 - 01/11/18 07:11 AM (6 years, 19 days ago)

Quote:

mushpunx said:
See how thick and white the myc looks? Sometimes it even gets a cottage cheese look. It will look solid like a block sometimes too.
This photo below is another good example


This is the only photo of clean spawn I have at the sec but you can see the difference.






Duly noted. Thanks


Extras: Filter Print Post Top
Invisiblebacillus
Very Serious Mushronomer
Male

Registered: 12/07/17
Posts: 394
Loc: Central Europe
Re: Photo of bacteria on plates [Re: LocN9ne]
    #24936357 - 01/23/18 02:11 PM (6 years, 7 days ago)

I think I've seen some of these. What are their names? Especially the dark green one in the upper right corner.



--------------------
Mycelium on agarLet's trade


Extras: Filter Print Post Top
InvisibleZiran
The Hero of Time
I'm a teapot User Gallery


Folding@home Statistics
Registered: 02/03/16
Posts: 6,030
Loc: Temple of Time
Re: Photo of bacteria on plates [Re: mushpunx]
    #24936377 - 01/23/18 02:24 PM (6 years, 7 days ago)

Quote:

mushpunx said:



2 of the plates were from a sloppy pour I guess, never poured a bad plate before :shrug:

Anyways a lot of folks ask me what bacteria look like on agar. So I figured I start a thread where people can put up photos of bacteria in plates and jars, mold colonies etc

What to look for

:thumbup:




your pictures are broken. :frown:

Quote:

DavieDonna said:
Quote:

Ziran said:
Some molds and bacterias. along with some cube mycelium





Whoa there's a lot going on here




Also, Here is the cleaned up plates that I did xfers on.





--------------------
Song Of Healing
:super: Updated Pf Tek Guide :super:
Ziran's Teks
AMU Q&A Thread
The Chinese word for nature is zìrán and it means that of which is of itself.



Extras: Filter Print Post Top
Offlinepacmanbreed
 User Gallery

Registered: 10/12/16
Posts: 3,659
Last seen: 1 year, 11 months
Re: Photo of bacteria on plates [Re: Ziran]
    #24937395 - 01/23/18 08:32 PM (6 years, 6 days ago)


New bacterial culture with ring and aery growth.

2 xfer away from that culture


Extras: Filter Print Post Top
InvisibleLocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
 User Gallery


Registered: 04/17/15
Posts: 7,076
Loc: to the brain Flag
Re: Photo of bacteria on plates [Re: mushpunx]
    #24942373 - 01/25/18 10:53 PM (6 years, 4 days ago)

Quote:

mushpunx said:


Yea I know, this thread is a couple years old I still don't have a supporter account yet




Don't need one now!!!

And here are some more...




--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


Extras: Filter Print Post Top
Invisibleeatyualive
Eat's You Alive :)
 User Gallery


Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
Trusted Cultivator
Re: Photo of bacteria on plates [Re: LocN9ne] * 1
    #24944076 - 01/26/18 04:34 PM (6 years, 4 days ago)

That looks like an egg cooked medium lol


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:


Extras: Filter Print Post Top
InvisibleLocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
 User Gallery


Registered: 04/17/15
Posts: 7,076
Loc: to the brain Flag
Re: Photo of bacteria on plates [Re: eatyualive]
    #24944744 - 01/26/18 08:38 PM (6 years, 3 days ago)

And the right picture in your signature looks like a whole tub of eggs


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


Extras: Filter Print Post Top
OfflineOericOss
Knowledgemuncher


Registered: 11/15/17
Posts: 119
Last seen: 1 year, 10 months
Re: Photo of bacteria on plates [Re: LocN9ne]
    #25079330 - 03/21/18 06:16 AM (5 years, 10 months ago)

What is this? Tried to swipe some wild allenii spores..


--------------------
 
      "Until you make the unconscious conscious, it will direct your life and you will call it faith."
- Jung


Extras: Filter Print Post Top
OfflineSebQc
Learning
Male

Registered: 01/06/18
Posts: 258
Loc: Canada Flag
Last seen: 2 years, 11 days
Re: Photo of bacteria on plates [Re: OericOss]
    #25079846 - 03/21/18 11:28 AM (5 years, 10 months ago)

Yes, i have some baterial plate.

All those plate were inoculated with 7 different MS syringe from the same vendor.

Let the show begin :eek:



--------------------



Extras: Filter Print Post Top
OfflineNotJonHamm
Stranger Danger

Registered: 11/14/17
Posts: 73
Loc: Canada Flag
Last seen: 4 years, 8 months
Re: Photo of bacteria on plates [Re: SebQc]
    #25079863 - 03/21/18 11:35 AM (5 years, 10 months ago)

Oh bacteria, I'm pretty good at growing that! :rasta:

 


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3 | 4  [ show all ]

Shop: Kraken Kratom Red Vein Kratom   PhytoExtractum Kratom Powder for Sale   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Bridgetown Botanicals Bridgetown Botanicals   North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Capsules


Similar ThreadsPosterViewsRepliesLast post
* WBS jar stalling after agar inoculation (photo) Josborne 866 14 05/04/21 02:29 PM
by Stipe-n Cap
* 3 Pie Plates legallyhomeless 660 10 08/03/07 01:15 AM
by legallyhomeless
* Agar emergency - myc growth but container has bacteria n the sides *DELETED* vinzy_a 1,320 10 09/28/06 08:06 AM
by vinzy_a
* Re: Plate Count Agar? Is this ready to use? shroomeritus 2,048 8 04/24/01 07:53 PM
by shroomeritus
* please help, brown slime like growth on agar plates decgrow4 2,826 8 02/27/08 02:15 AM
by CureCat
* First experience w/ bulk substrate, casing layer (photos) uncle_rico 10,857 10 08/24/11 11:25 AM
by Meetzu
* Whats with my agar plates? Frankenstein 8,600 8 09/08/22 03:46 PM
by schpat
* Bacteria agentjrr 1,175 5 03/24/04 02:46 AM
by agentjrr

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
8,074 topic views. 37 members, 292 guests and 50 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.065 seconds spending 0.011 seconds on 14 queries.