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Offlinemushpunx
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Re: Photo of bacteria on plates [Re: bodhisatta]
    #21868382 - 06/28/15 12:33 PM (8 years, 7 months ago)

A few months ago I was running into what I think was Aspergillus, it is a nasty one too. A little darker green than trich and it seemed to pretty much start off on the very surface as little green speckles.. instead of white like trich. It would turn myc yellow brown wherever it touched it.
Not as nasty as trich but certainly ruined a few tubs.
It still turns up every couple grows too


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OfflineGreen Bastard
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Re: Photo of bacteria on plates [Re: bodhisatta]
    #21868658 - 06/28/15 01:45 PM (8 years, 7 months ago)

Quote:

bodhisatta said:

Penicllin sp. I believe.


Left cocci bacteria
Right aspergillus




A question, Bodhista. The plate on the right... Is the mycelium also contaminated?
I have a few plates, on their 3rd and 4th transfers away from clones, with no glaringly obvious contams, with big differentials like that...


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InvisiblebodhisattaMDiscordReddit
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Re: Photo of bacteria on plates [Re: Green Bastard]
    #21868659 - 06/28/15 01:46 PM (8 years, 7 months ago)

No


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OfflineGreen Bastard
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Re: Photo of bacteria on plates [Re: bodhisatta]
    #21868689 - 06/28/15 01:52 PM (8 years, 7 months ago)

Thank you for the fast, and reassuring reply! Just aggressive growth, eh? Something to keep transferring from then.... :smile:


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OfflineDhearic
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Re: Photo of bacteria on plates [Re: Green Bastard]
    #21874192 - 06/29/15 03:59 PM (8 years, 6 months ago)







:whippedcreamhead:


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Offlinehamloaf
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Re: Photo of bacteria on plates [Re: Dhearic]
    #21874208 - 06/29/15 04:04 PM (8 years, 6 months ago)

DEATHSTAR MANIA!  :deathstar:


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InvisiblebodhisattaMDiscordReddit
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Re: Photo of bacteria on plates [Re: hamloaf]
    #21874227 - 06/29/15 04:10 PM (8 years, 6 months ago)

got a plate of clostridium today fucking smelled like feta cheese in the most unpleasant way.


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InvisibleFriedEggS
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Re: Photo of bacteria on plates [Re: Dhearic]
    #21874312 - 06/29/15 04:36 PM (8 years, 6 months ago)

Quote:

Dhearic said:





that top left section kinda looks like the head of a baby when viewed through an ultrasound


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Invisiblejbaby007
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Re: Photo of bacteria on plates [Re: FriedEgg]
    #21874386 - 06/29/15 04:55 PM (8 years, 6 months ago)

I should have saved my plate from earlier today and taken a pic. It had a nasty chunk of bacteria on it. Thank god I've gotten into agar. It's so nice knowing that I can just transfer healthy mycelium away onto another plate and not worry about it.


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OfflineKizzle
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Re: Photo of bacteria on plates [Re: jbaby007]
    #21874543 - 06/29/15 05:27 PM (8 years, 6 months ago)


Sneaky bacteria


Not so sneaky bacteria


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InvisibleMad Season
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Re: Photo of bacteria on plates [Re: Kizzle]
    #21874565 - 06/29/15 05:31 PM (8 years, 6 months ago)

Super sneaky.. where the hell is it kizzle lol. How do you know it has bacteria?

Is it the off center circle taking up almost all of the dish?


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Edited by Mad Season (06/29/15 05:33 PM)


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OfflineKizzle
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Re: Photo of bacteria on plates [Re: Mad Season]
    #21874650 - 06/29/15 05:49 PM (8 years, 6 months ago)

It's the very light film covering the "uncolonized" area. I don't know how many dishes like that slipped by before I finally noticed it but I started to wonder why the mycelium was suddenly stalling instead of colonizing the whole plate :lol:


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Offlinebburnett227
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Re: Photo of bacteria on plates [Re: Kizzle]
    #21908979 - 07/07/15 10:00 AM (8 years, 6 months ago)

When ur transferring out from the contaminated plate to a new one, do u cut all of the good myc out for the transfer? Or just a wedge. I'm under the impression the goal here is to have a plate of full myc, so I would think you'd cut all of it....haven't dine agar yet, 2 or 3 more days til clones are  ready, and just wanna be sure I'm at least starting it right.


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Offlinemushpunx
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Re: Photo of bacteria on plates [Re: bburnett227]
    #21913513 - 07/08/15 05:22 AM (8 years, 6 months ago)

Quote:

bburnett227 said:
When ur transferring out from the contaminated plate to a new one, do u cut all of the good myc out for the transfer? Or just a wedge. I'm under the impression the goal here is to have a plate of full myc, so I would think you'd cut all of it....haven't dine agar yet, 2 or 3 more days til clones are  ready, and just wanna be sure I'm at least starting it right.





Just a timy wedge dude. Like a grain of rice. This way you can cut away from contams, isolate genetics etc..

It will grow out on the new plat


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Offlinebburnett227
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Re: Photo of bacteria on plates [Re: mushpunx]
    #21913574 - 07/08/15 06:04 AM (8 years, 6 months ago)

thank you sir


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OfflineWill7401
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Re: Photo of bacteria on plates [Re: FriedEgg]
    #23134139 - 04/19/16 01:16 PM (7 years, 9 months ago)

What is this? I am getting this from time to time but I am really noticing it with the two Cam MS I purchased.


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InvisiblePastywhyteMDiscord
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Re: Photo of bacteria on plates [Re: Will7401]
    #23134152 - 04/19/16 01:23 PM (7 years, 9 months ago)

That is bacteria.


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InvisibleLocN9ne
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Re: Photo of bacteria on plates [Re: Pastywhyte]
    #23134160 - 04/19/16 01:25 PM (7 years, 9 months ago)

Quote:

Pastywhyte said:
That is bacteria.



Af


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Offlinegeorgeballer
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Re: Photo of bacteria on plates [Re: Will7401]
    #24115720 - 02/24/17 09:50 AM (6 years, 10 months ago)

Quote:

Will7401 said:
What is this? I am getting this from time to time but I am really noticing it with the two Cam MS I purchased.




Is that green a good color for spotting bacteria?


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OfflineDyn-A-Mite
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Re: Photo of bacteria on plates [Re: Kizzle]
    #24115767 - 02/24/17 10:05 AM (6 years, 10 months ago)

Quote:

Kizzle said:

Sneaky bacteria


Not so sneaky bacteria




Kizzle that is a perfect example of how bacteria can hitch a ride on seamlessly healthy looking grain. Thanks for providing such an informative picture.


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