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Offlinemushpunx
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Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 14 hours
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Photo of bacteria on plates
    #21865514 - 06/27/15 06:45 PM (8 years, 7 months ago)




2 of the plates were from a sloppy pour I guess, never poured a bad plate before :shrug:

Anyways a lot of folks ask me what bacteria look like on agar. So I figured I start a thread where people can put up photos of bacteria in plates and jars, mold colonies etc

What to look for

:thumbup:


--------------------

Amateur Mycologists United
AMU Q&A


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InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: Photo of bacteria on plates [Re: mushpunx]
    #21865532 - 06/27/15 06:48 PM (8 years, 7 months ago)

:aaaargh:


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Fail to plan and you plan to fail.

Enter the Ban Lottery


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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Registered: 04/30/13
Posts: 61,889
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Re: Photo of bacteria on plates [Re: Buck513]
    #21865881 - 06/27/15 07:48 PM (8 years, 7 months ago)


Penicllin sp. I believe.


Left cocci bacteria
Right aspergillus


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InvisibleMad Season
hookers and blackjack
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Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
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Re: Photo of bacteria on plates [Re: bodhisatta]
    #21866334 - 06/27/15 09:29 PM (8 years, 7 months ago)

Guess how many different species of bacteria is on this plate:

Tissue sample from clone gone wrong :rofl: and that folks is why I make multiple dishes when cloning.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflinePussyFart
Retired Cultivation Extrodinaire
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Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
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Re: Photo of bacteria on plates [Re: Mad Season]
    #21866367 - 06/27/15 09:39 PM (8 years, 7 months ago)

That my friend is why we take tissue samples from inside the fruit, as to not grab anything that a contam has landed on....lol.


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Invisibled0urd3n
Just call me "D"

Registered: 09/15/10
Posts: 5,237
Re: Photo of bacteria on plates [Re: PussyFart]
    #21866399 - 06/27/15 09:46 PM (8 years, 7 months ago)

Bacteria + some purple mold



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OfflinePussyFart
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Registered: 04/08/12
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Re: Photo of bacteria on plates [Re: d0urd3n]
    #21866418 - 06/27/15 09:50 PM (8 years, 7 months ago)

         


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InvisibleMad Season
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Registered: 09/16/12
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Re: Photo of bacteria on plates [Re: PussyFart]
    #21866719 - 06/27/15 11:39 PM (8 years, 7 months ago)

Haha PF I do that. I just messed up somehow :P a different plate from the same stem went swimmingly though. It was a small ass stem. Even the base was a cm tops.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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InvisibleFriedEggS
I'm a teapot


Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
Re: Photo of bacteria on plates [Re: Mad Season]
    #21866888 - 06/28/15 12:53 AM (8 years, 7 months ago)

good idea for a thread.


here's my contribution:


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(Yes, the egg is real)

How to post pics


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Offlinehamloaf
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Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
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Re: Photo of bacteria on plates [Re: mushpunx]
    #21867214 - 06/28/15 04:24 AM (8 years, 7 months ago)

:deathstar:


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


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Invisibleblindingleaf
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Registered: 07/19/13
Posts: 22,008
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Re: Photo of bacteria on plates [Re: hamloaf]
    #21867230 - 06/28/15 04:46 AM (8 years, 7 months ago)



--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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OfflineGreenRabbit
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Registered: 04/28/13
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Re: Photo of bacteria on plates [Re: bodhisatta]
    #21867819 - 06/28/15 09:55 AM (8 years, 7 months ago)

Quote:

bodhisatta said:

Left cocci bacteria
Right aspergillus




That aspergillus looks just like trich to me. How can you tell the difference?


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InvisiblebodhisattaMDiscordReddit
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Re: Photo of bacteria on plates [Re: GreenRabbit]
    #21867833 - 06/28/15 09:59 AM (8 years, 7 months ago)

Trich never looks like that on plates in my experience. Also rarely does it turn green on a plate if it's growing with mycelium too.


Edited by Trusted cuItivator (06/28/15 09:59 AM)


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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
Posts: 37,810
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Re: Photo of bacteria on plates [Re: GreenRabbit]
    #21867837 - 06/28/15 10:00 AM (8 years, 7 months ago)

Trich usually has a crystaline texture to the surface and a bubbling growth pattern. Also like bodhi said it usually needs some decent FAE to sporulate.


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InvisibleMad Season
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Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
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Re: Photo of bacteria on plates [Re: GreenRabbit]
    #21867852 - 06/28/15 10:05 AM (8 years, 7 months ago)

On substrates though powder green look can indeed be a multitude of species and even a few different families. Aspergillus, penicillium, trichoderma.. They all can range in different shades too. The online mushroom community generally calls them all trich. Realistically speaking a microscope would for sure tell what it is.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Offlinehamloaf
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Registered: 12/23/09
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Re: Photo of bacteria on plates [Re: Mad Season]
    #21867875 - 06/28/15 10:12 AM (8 years, 7 months ago)

All three of the aforementioned contam species have their own shade of green.  Been blessed to not run into too much Aspergillus, but have seen a fair share of penicillum and trichoderma.  Penicillum is a teal green, where trichoderma is a dark, deep, forest green color.  Very distinct.  Trichoderma is the bane if the mushroom culture.  HATE!

:deathstar:


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


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OfflineGreenRabbit
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Re: Photo of bacteria on plates [Re: bodhisatta]
    #21867887 - 06/28/15 10:16 AM (8 years, 7 months ago)

Quote:

bodhisatta said:
Trich never looks like that on plates in my experience. Also rarely does it turn green on a plate if it's growing with mycelium too.




Sweet, good to know. Just started agar so I've only seen trich in PF jars. Pretty sure it was trich everytime


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InvisibleMad Season
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Re: Photo of bacteria on plates [Re: hamloaf]
    #21867900 - 06/28/15 10:21 AM (8 years, 7 months ago)

Interesting. I've also seen a bright green/mossy green look in certain species of trich. However I know what you mean by the teal look in penicillium. I've seen that quite a bit too.

So looking at bods pic, I can see it's a very fine powdery look. Almost looks like a smooth surface, whereas trich has more visible particle/granite look. Is this a key distinction between the 2 on substrates? Also another distinction as mentioned before is that it doesn't sporulate on plates well. Just curious how you guys visually tell the difference. *runs off to look at pics of them*


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (06/28/15 10:23 AM)


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Offlinehamloaf
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Registered: 12/23/09
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Re: Photo of bacteria on plates [Re: Mad Season]
    #21867932 - 06/28/15 10:33 AM (8 years, 7 months ago)

I don't know what the guys are talking about over the issue of trich not sporulating on agar very often, because I'v had trich break out on several plates over the last 6 years.  Recently had a contam breakout in the lab.  40 plus pre-poured plates that were sitting for over a month and a half ALL had trich in them.  Don't know how long said trich was growing on the plates for.  What is known is as soon as the contam was discovered it was immediately discarded.  Black mold is another that'll sporulate all over your agar media.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


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InvisiblebodhisattaMDiscordReddit
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Registered: 04/30/13
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Re: Photo of bacteria on plates [Re: hamloaf]
    #21868200 - 06/28/15 11:54 AM (8 years, 7 months ago)

Quote:

hamloaf said:
All three of the aforementioned contam species have their own shade of green.  Been blessed to not run into too much Aspergillus, but have seen a fair share of penicillum and trichoderma.  Penicillum is a teal green, where trichoderma is a dark, deep, forest green color.  Very distinct.  Trichoderma is the bane if the mushroom culture.  HATE!

:deathstar:



Actually different species of each have a multitude of colors.


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