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OfflineKevwynn
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How do i sterilize needles..
    #21864685 - 06/27/15 02:57 PM (8 years, 7 months ago)

If I make a lc. How do i sterilize the inside of the needle that ill be using for my wbs jars?


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Invisibleuncle_rico
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Re: How do i sterilize needles.. [Re: Kevwynn]
    #21864772 - 06/27/15 03:21 PM (8 years, 7 months ago)

ghetto way (which is very effective) is to suck up boiling water, purge, suck up, purge, suck up .... then before use ... purge ... and then flame sterilize.

or use your PC instead of the boiling water

edit: flame sterilization (where the needle is red) is going to work on the outside and inside of the needle.


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Edited by uncle_rico (06/27/15 03:23 PM)


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Invisibleazur
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Re: How do i sterilize needles.. [Re: uncle_rico]
    #21864791 - 06/27/15 03:27 PM (8 years, 7 months ago)

Yup. In pc on top if your grain jars. LOOSELY wrap it in foil and pc with jars


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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OfflineMoxyOx
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Re: How do i sterilize needles.. [Re: azur]
    #21864885 - 06/27/15 04:02 PM (8 years, 7 months ago)

Are we talking syringe or actual needle?


--------------------
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The path the ancients cleared has closed.
And the other path, everyone's path,
easy and wide, goes nowhere.
I am alone and find my way.


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Invisibleazur
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Re: How do i sterilize needles.. [Re: MoxyOx]
    #21864887 - 06/27/15 04:03 PM (8 years, 7 months ago)

Both all together


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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OfflineMoxyOx
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Re: How do i sterilize needles.. [Re: azur]
    #21864914 - 06/27/15 04:10 PM (8 years, 7 months ago)

Your method sounds most convenient then. Do you draw in air to the syringe before you throw it in?


--------------------
No one behind, no one ahead.
The path the ancients cleared has closed.
And the other path, everyone's path,
easy and wide, goes nowhere.
I am alone and find my way.


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Invisibleazur
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Re: How do i sterilize needles.. [Re: MoxyOx]
    #21864948 - 06/27/15 04:19 PM (8 years, 7 months ago)

Nope

What i like about my method is the entire rig is sterilized.


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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InvisibleMad Season
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Re: How do i sterilize needles.. [Re: azur]
    #21865116 - 06/27/15 05:12 PM (8 years, 7 months ago)

Yeah you don't need water drawn in syringes. If you need sterile water just pc it in a jar and do the blank/empty syringes in foil or another jar.

Azurs method is very convenient if you're running other things, which let's not joke around, you should be running things. If not get on that.


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Edited by Mad Season (06/27/15 05:13 PM)


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OfflineBLINKfan420
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Re: How do i sterilize needles.. [Re: Mad Season]
    #21865241 - 06/27/15 05:43 PM (8 years, 7 months ago)

Will all syringes withstand a 15psi PC cycle?

BLiNK


--------------------



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Edited by BLINKfan420 (06/27/15 05:43 PM)


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Invisibleazur
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Re: How do i sterilize needles.. [Re: BLINKfan420]
    #21865248 - 06/27/15 05:45 PM (8 years, 7 months ago)

I don't know about ALL syringes. But vendor type will If wrapped loosely in foil or in a jar. I'd estimate i will last a good 30-50 ninety minute 15psi cycles before the plunger wears away


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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OfflineMoxyOx
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Re: How do i sterilize needles.. [Re: azur]
    #21867145 - 06/28/15 03:30 AM (8 years, 7 months ago)

I'd figure that the plastic material would melt somewhere around mid 300's and PC's only go up to like 250. It shouldn't melt unless it's from China.


--------------------
No one behind, no one ahead.
The path the ancients cleared has closed.
And the other path, everyone's path,
easy and wide, goes nowhere.
I am alone and find my way.


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OfflineKevwynn
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Re: How do i sterilize needles.. [Re: MoxyOx]
    #21868220 - 06/28/15 11:58 AM (8 years, 7 months ago)

Alright and what size syringe/needle will i need to suck up the mycelium?


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Invisibleazur
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Re: How do i sterilize needles.. [Re: Kevwynn]
    #21868223 - 06/28/15 11:59 AM (8 years, 7 months ago)

I use vendor's syringes for lc's


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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OfflineKevwynn
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Re: How do i sterilize needles.. [Re: azur]
    #21868608 - 06/28/15 01:31 PM (8 years, 7 months ago)

No idea what gauge?


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OfflineGreen Bastard
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Re: How do i sterilize needles.. [Re: azur]
    #21868622 - 06/28/15 01:35 PM (8 years, 7 months ago)

16 g x 1 1/2" needle  works well for me.


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InvisibleJuiceh
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Re: How do i sterilize needles.. [Re: Kevwynn]
    #21868648 - 06/28/15 01:41 PM (8 years, 7 months ago)

Quote:

Kevwynn said:
Alright and what size syringe/needle will i need to suck up the mycelium?



Use 16 gauge needles for LC or bigger. Anything smaller has a much higher chance of clogging on a chunk of myceluim. Spore syringes can get away with much smaller needles. Most vendors will give you 18 gauge needles, though it seems to be sometimes random and you get whatever needles they have in stock because I've got 16 and 18 gauge from the same vendor. If you have a stirbar in your LC give it a real good spin to break up the mycelium before you go to suck up some LC. Some species produce some really thick LC. The morel cultures I'm currently working with can be difficult to pick up even with a 16 gauge.


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OfflineKevwynn
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Re: How do i sterilize needles.. [Re: Juiceh]
    #21872518 - 06/29/15 09:27 AM (8 years, 6 months ago)

Thank u everybody :smile:


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