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Jim Morrison
Lizard King



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Lion's Mane Mycelium on Wheat Berries
#21864463 - 06/27/15 01:57 PM (8 years, 7 months ago) |
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I inoculated 5 one pound bags of sterilized wheat berries with 2 mL of LC 9 days ago, and I really can't seem to see any mycelium yet. I know that lion's mane mycelium is really wispy at first, but shouldn't I be able to see something by now?
-------------------- Jim Morrison's Trade List Live Long and Myceliate! The only power any government has is the power to crack down on criminals. Well, when there aren't enough criminals, one makes them. One declares so many things to be a crime that it becomes impossible for men to live without breaking laws. Ayn Rand
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drake89
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Re: Lion's Mane Mycelium on Wheat Berries [Re: Jim Morrison]
#21865399 - 06/27/15 06:20 PM (8 years, 7 months ago) |
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That's a lot of grain and not much LC. So be patient :p
If you don't see ANYTHiNg at two weeks...well at least you're not growing mold!
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
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Re: Lion's Mane Mycelium on Wheat Berries [Re: drake89]
#21865894 - 06/27/15 07:51 PM (8 years, 7 months ago) |
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I would use at least 10 to 20cc for a pound bag if not more
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Jim Morrison
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Re: Lion's Mane Mycelium on Wheat Berries [Re: bodhisatta]
#21866040 - 06/27/15 08:23 PM (8 years, 7 months ago) |
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Quote:
bodhisatta said: I would use at least 10 to 20cc for a pound bag if not more
Wouldn't that introduce too much liquid into the bag? The instructions with the bags (and I have seen elsewhere) recommend up to 6 mL, but not much more for that reason. In hind sight, I should have maybe used at least 4 mL. At least there is no mold or contaminants. Maybe it will just take a little longer this way.
-------------------- Jim Morrison's Trade List Live Long and Myceliate! The only power any government has is the power to crack down on criminals. Well, when there aren't enough criminals, one makes them. One declares so many things to be a crime that it becomes impossible for men to live without breaking laws. Ayn Rand
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bodhisatta 
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Posts: 61,889
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Re: Lion's Mane Mycelium on Wheat Berries [Re: Jim Morrison]
#21866057 - 06/27/15 08:25 PM (8 years, 7 months ago) |
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If you use LC make your bags so you compensate for the liquid.
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Jim Morrison
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Re: Lion's Mane Mycelium on Wheat Berries [Re: drake89]
#21866064 - 06/27/15 08:25 PM (8 years, 7 months ago) |
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Quote:
drake89 said: That's a lot of grain and not much LC. So be patient :p
If you don't see ANYTHiNg at two weeks...well at least you're not growing mold!
Ohhhhh. I hope it didn't sound like I used only 2 mL for all five bags! I put 2 mL into each bag, just to clarify.
-------------------- Jim Morrison's Trade List Live Long and Myceliate! The only power any government has is the power to crack down on criminals. Well, when there aren't enough criminals, one makes them. One declares so many things to be a crime that it becomes impossible for men to live without breaking laws. Ayn Rand
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drake89
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Re: Lion's Mane Mycelium on Wheat Berries [Re: Jim Morrison]
#21866419 - 06/27/15 09:50 PM (8 years, 7 months ago) |
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Naw LM myc is very fine until it starts to pin. Like single cell thick hyphae extending inches, it looks like actual cob webs only visible in good lighting.
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knomadic_niki
A mile high



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Re: Lion's Mane Mycelium on Wheat Berries [Re: drake89]
#21869093 - 06/28/15 03:34 PM (8 years, 7 months ago) |
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sometimes i make lion's mane transfers on agar and i can't see the growth at all (i guess that's why some people use colored agar). then they surprise me a few weeks later when they start pinning on the plate. it can be hard to see, be patient
-------------------- My trade list In search of sporeless oyster cultures
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Jim Morrison
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Re: Lion's Mane Mycelium on Wheat Berries [Re: knomadic_niki]
#21869515 - 06/28/15 05:12 PM (8 years, 7 months ago) |
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Thank you for the feedback everyone! I think I see a little mycelium in a few places. Man is it fine! I used a light and shined it from the side. Hope it all turns out. I tried lion's mane about a month ago, and I loved it!
-------------------- Jim Morrison's Trade List Live Long and Myceliate! The only power any government has is the power to crack down on criminals. Well, when there aren't enough criminals, one makes them. One declares so many things to be a crime that it becomes impossible for men to live without breaking laws. Ayn Rand
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Jim Morrison
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Re: Lion's Mane Mycelium on Wheat Berries [Re: Jim Morrison]
#21875123 - 06/29/15 07:13 PM (8 years, 6 months ago) |
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Very visible growth on several of the bags! Looking good!
-------------------- Jim Morrison's Trade List Live Long and Myceliate! The only power any government has is the power to crack down on criminals. Well, when there aren't enough criminals, one makes them. One declares so many things to be a crime that it becomes impossible for men to live without breaking laws. Ayn Rand
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RogerSmith

Registered: 01/29/15 
Posts: 365
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Re: Lion's Mane Mycelium on Wheat Berries [Re: Jim Morrison]
#21875368 - 06/29/15 08:01 PM (8 years, 6 months ago) |
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Light is useful for spotting thin mycelium, I do that too. Do you plan to mix grains with sterilized or pasteurized sub?
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foodsgoodtoo
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Re: Lion's Mane Mycelium on Wheat Berries [Re: Jim Morrison]
#21876350 - 06/30/15 01:52 AM (8 years, 6 months ago) |
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thats good to hear. my liquid culture almost froze solid but i still used it but ran into other problems.
now i have a new batch just in and used some more of the syringe.
good luck to yours.
--------------------
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Jim Morrison
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Re: Lion's Mane Mycelium on Wheat Berries [Re: RogerSmith]
#21876653 - 06/30/15 05:41 AM (8 years, 6 months ago) |
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Quote:
RogerSmith said: Light is useful for spotting thin mycelium, I do that too. Do you plan to mix grains with sterilized or pasteurized sub?
I plan on experimenting a little with it. I will use some on pasteurized straw. Some to do 1 gallon zip lock bags with hardwood sawdust pellets. And some with a mix of sawdust pellets and straw.
-------------------- Jim Morrison's Trade List Live Long and Myceliate! The only power any government has is the power to crack down on criminals. Well, when there aren't enough criminals, one makes them. One declares so many things to be a crime that it becomes impossible for men to live without breaking laws. Ayn Rand
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Jim Morrison
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Re: Lion's Mane Mycelium on Wheat Berries [Re: foodsgoodtoo]
#21876655 - 06/30/15 05:42 AM (8 years, 6 months ago) |
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Quote:
foodsgoodtoo said: thats good to hear. my liquid culture almost froze solid but i still used it but ran into other problems.
now i have a new batch just in and used some more of the syringe.
good luck to yours.
Did it contaminate? I hope your new batch does good!
-------------------- Jim Morrison's Trade List Live Long and Myceliate! The only power any government has is the power to crack down on criminals. Well, when there aren't enough criminals, one makes them. One declares so many things to be a crime that it becomes impossible for men to live without breaking laws. Ayn Rand
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foodsgoodtoo
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Re: Lion's Mane Mycelium on Wheat Berries [Re: Jim Morrison]
#21879738 - 06/30/15 07:27 PM (8 years, 6 months ago) |
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actually the bird seed was fermented quite so.,
though i managed to have some mycelium from it it all went ontop of the dead grass.
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Jim Morrison
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Re: Lion's Mane Mycelium on Wheat Berries [Re: foodsgoodtoo]
#21891464 - 07/03/15 06:33 AM (8 years, 6 months ago) |
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Hmmmm. My bags are kind of smelling just a little sour/moldy. Maybe just my imagination. Trying to smell through the filter patch. I don't see any obvious contamination. Twelve days since inoculating and there is some mycelium, but...
-------------------- Jim Morrison's Trade List Live Long and Myceliate! The only power any government has is the power to crack down on criminals. Well, when there aren't enough criminals, one makes them. One declares so many things to be a crime that it becomes impossible for men to live without breaking laws. Ayn Rand
Edited by Jim Morrison (07/03/15 06:34 AM)
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Jim Morrison
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Re: Lion's Mane Mycelium on Wheat Berries [Re: Jim Morrison]
#21891556 - 07/03/15 07:14 AM (8 years, 6 months ago) |
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Maybe I just don't know how lions mane mycelium smells.
-------------------- Jim Morrison's Trade List Live Long and Myceliate! The only power any government has is the power to crack down on criminals. Well, when there aren't enough criminals, one makes them. One declares so many things to be a crime that it becomes impossible for men to live without breaking laws. Ayn Rand
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foodsgoodtoo
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Re: Lion's Mane Mycelium on Wheat Berries [Re: Jim Morrison]
#21892375 - 07/03/15 12:11 PM (8 years, 6 months ago) |
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it smells identical to other myc pretty sure
--------------------
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Jim Morrison
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Re: Lion's Mane Mycelium on Wheat Berries [Re: foodsgoodtoo]
#21892523 - 07/03/15 12:46 PM (8 years, 6 months ago) |
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Quote:
foodsgoodtoo said: it smells identical to other myc pretty sure
Yeah that's pretty much what I thought. I guess I'll just let it keep growing out. And see what happens. It's in a totally sealed bag, so it shouldn't spread contamination to anything nearby.
-------------------- Jim Morrison's Trade List Live Long and Myceliate! The only power any government has is the power to crack down on criminals. Well, when there aren't enough criminals, one makes them. One declares so many things to be a crime that it becomes impossible for men to live without breaking laws. Ayn Rand
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foodsgoodtoo
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Re: Lion's Mane Mycelium on Wheat Berries [Re: Jim Morrison]
#21892985 - 07/03/15 02:45 PM (8 years, 6 months ago) |
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my bags arent looking too great. esp for lc. mmm shouldve just kept the syringe out the fridge
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Toadstool5
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Re: Lion's Mane Mycelium on Wheat Berries [Re: foodsgoodtoo]
#21893555 - 07/03/15 05:01 PM (8 years, 6 months ago) |
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Quote:
Light is useful for spotting thin mycelium, I do that too.
Yeah and even a small hand-held magnifying glass works great. I use a small LED light and my 40x magnifying glass.
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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Jim Morrison
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Re: Lion's Mane Mycelium on Wheat Berries [Re: Toadstool5]
#21893712 - 07/03/15 05:48 PM (8 years, 6 months ago) |
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Quote:
Toadstool5 said:
Quote:
Light is useful for spotting thin mycelium, I do that too.
Yeah and even a small hand-held magnifying glass works great. I use a small LED light and my 40x magnifying glass.
That's a great idea! I have a jeweler's loupe that I can try.
-------------------- Jim Morrison's Trade List Live Long and Myceliate! The only power any government has is the power to crack down on criminals. Well, when there aren't enough criminals, one makes them. One declares so many things to be a crime that it becomes impossible for men to live without breaking laws. Ayn Rand
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Toadstool5
A Registered Mycophile



Registered: 01/22/15
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Re: Lion's Mane Mycelium on Wheat Berries [Re: Jim Morrison]
#21894019 - 07/03/15 07:02 PM (8 years, 6 months ago) |
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Worked for me just this morning
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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foodsgoodtoo
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Re: Lion's Mane Mycelium on Wheat Berries [Re: Toadstool5]
#21895495 - 07/04/15 03:19 AM (8 years, 6 months ago) |
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i suppose i could make another thread on this but , here was a salvaged piece of the bags that were thrown out

really thought the lc was done for so had this as a possible back up if they came through.
opened the spawn and didnt think it was cobweb, but looking at the photo is looks like a contam -
maybe thats just the thin portion
--------------------
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Toadstool5
A Registered Mycophile



Registered: 01/22/15
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Re: Lion's Mane Mycelium on Wheat Berries [Re: foodsgoodtoo]
#21896779 - 07/04/15 01:37 PM (8 years, 6 months ago) |
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Some of it does look suspiciously grayish but that could be the lighting
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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Jim Morrison
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Re: Lion's Mane Mycelium on Wheat Berries [Re: Toadstool5]
#21899888 - 07/05/15 07:00 AM (8 years, 6 months ago) |
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Well, out of 5 one pound bags, 2 look really good. 1 I can actually see very very wispy mycelium with a 25X jeweler's loupe. 1 was colonizing only in the middle, because I didn't mix it when I inoculated, so I mixed it today. And 1 that I inoculated just in the middle and mixed 4 days ago doesn't show anything I can see yet. I'm not too sure I like working with lion's mane mycelium. Because it is so hard to see at first and I'm not experienced at all it makes me really anxious! I have a pink oyster LC syringe from Sporeworks coming, hopefully my rye berries from Outgrow are still good after siting in the fridge for 6 days. Next time I will order the syringe first and wait 3 days to order the rye berry bags. All of the post I've seen says that Sporeworks is super fast, but not in my experience. The 2 times I have ordered, it took at least a week each time to get my order. I still think they are a great company though. I just have to plan better. Anyway, I hope the pink oyster is easier to see when colonizing.
-------------------- Jim Morrison's Trade List Live Long and Myceliate! The only power any government has is the power to crack down on criminals. Well, when there aren't enough criminals, one makes them. One declares so many things to be a crime that it becomes impossible for men to live without breaking laws. Ayn Rand
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Jim Morrison
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Re: Lion's Mane Mycelium on Wheat Berries [Re: Jim Morrison]
#21948720 - 07/15/15 06:14 PM (8 years, 6 months ago) |
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These bags aren't doing so well. I got two of them to colonize pretty well and used them to make two fuel pellet bags. Of the other three, one looks like it's trying to do something but not really consolidating too well and the other two don't look like they are doing much of anything. It's been almost a month now. Guess I'll just let them sit. They aren't contaminated so..... I got the bags of wheat berries from Outgrow and they looked a little dry. I wonder if it's a moisture issue. Really disappointing.
-------------------- Jim Morrison's Trade List Live Long and Myceliate! The only power any government has is the power to crack down on criminals. Well, when there aren't enough criminals, one makes them. One declares so many things to be a crime that it becomes impossible for men to live without breaking laws. Ayn Rand
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Jim Morrison
Lizard King



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Re: Lion's Mane Mycelium on Wheat Berries [Re: Jim Morrison]
#21948817 - 07/15/15 06:35 PM (8 years, 6 months ago) |
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Since I don't seem to have much luck in growing my own grain spawn from LC and pre-sterilized grain bags, I just decided to order premade spawn. With the LC and bags it was right around $60 for 6 one pound bags. I just ordered 5 pounds of pink oyster spawn for 43.50. Why was I trying to make my own!?!
-------------------- Jim Morrison's Trade List Live Long and Myceliate! The only power any government has is the power to crack down on criminals. Well, when there aren't enough criminals, one makes them. One declares so many things to be a crime that it becomes impossible for men to live without breaking laws. Ayn Rand
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foodsgoodtoo
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Re: Lion's Mane Mycelium on Wheat Berries [Re: Jim Morrison]
#21949044 - 07/15/15 07:34 PM (8 years, 6 months ago) |
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my bags failed too.
im not quitting though it feels unfair but gives you supplies that ould last and last
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Toadstool5
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Re: Lion's Mane Mycelium on Wheat Berries [Re: Jim Morrison]
#21949178 - 07/15/15 08:08 PM (8 years, 6 months ago) |
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Presterilized spawn normally has issues even when done correctly. Sterilization isnt complete, it just gives us enough time to colonize it before contams kick in. With shipping time and all the handling the spawn goes through I wouldnt be surprised if it always arrived contaminated. 
Get a pressure cooker and do your own grains, use them immediately out of the PC the next day when they are cool, always use flame sterilization, always use a SAB, and bleach bomb the room if you have mold issues.
Its amazingly cheaper to do your own and not too difficult unless you plan on making 100's of pounds of spawn for a massive grow. 
Making grain jar lids is a pain in the ass though
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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