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Offlinerxb
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i cleaned a mold contaminated live culture and saved it. (might have useful applications)
    #21860972 - 06/26/15 05:28 PM (8 years, 7 months ago)

i took a live culture which was purposefully contaminated with mold.

and i dropped some on agar in a jar with a SHIP.

then i waited until the plate was mostly mold

and i injected 6cc's 35% hydrogen peroxide.

and shook it.

which dislodged the agar quite a bit unfortunately

waited 2 weeks.

agar seems to be colonizing with healthy non-mold myc

no attempts to repeat the results, just sharing something i found interesting and potentially useful.

not sure why myc was more resilient than the mold, im kind of surprized


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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OfflinePsilosopherr
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Re: i cleaned a mold contaminated live culture and saved it. (might have useful applications) [Re: rxb]
    #21861052 - 06/26/15 05:52 PM (8 years, 7 months ago)

Quote:

rxb said:
i took a live culture which was purposefully contaminated with mold.

and i dropped some on agar in a jar with a SHIP.

then i waited until the plate was mostly mold

and i injected 6cc's 35% hydrogen peroxide.

and shook it.

which dislodged the agar quite a bit unfortunately

waited 2 weeks.

agar seems to be colonizing with healthy non-mold myc

no attempts to repeat the results, just sharing something i found interesting and potentially useful.

not sure why myc was more resilient than the mold, im kind of surprized



wowza. very cool rxb :thumbup:

I'll have to give that a shot

So there IS a use for hydrogen peroxide in mushy cultivation.


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Offlinerxb
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Re: i cleaned a mold contaminated live culture and saved it. (might have useful applications) [Re: Psilosopherr]
    #21861327 - 06/26/15 07:17 PM (8 years, 7 months ago)

Quote:

rbalzer said:
Quote:

rxb said:
i took a live culture which was purposefully contaminated with mold.

and i dropped some on agar in a jar with a SHIP.

then i waited until the plate was mostly mold

and i injected 6cc's 35% hydrogen peroxide.

and shook it.

which dislodged the agar quite a bit unfortunately

waited 2 weeks.

agar seems to be colonizing with healthy non-mold myc

no attempts to repeat the results, just sharing something i found interesting and potentially useful.

not sure why myc was more resilient than the mold, im kind of surprized



wowza. very cool rxb :thumbup:

I'll have to give that a shot

So there IS a use for hydrogen peroxide in mushy cultivation.




well i doubt much HP has been tried at 35% at all.

im curious as to why the myc survived and the mold did not.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Invisiblemaddchef
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Re: i cleaned a mold contaminated live culture and saved it. (might have useful applications) [Re: rxb]
    #21861785 - 06/26/15 09:45 PM (8 years, 6 months ago)

Is this an lc or tissue sample that was used? Please give details on the age and whatnots


--------------------
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All mushrooms are edible, but some only once.....                     

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InvisiblebodhisattaMDiscordReddit
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Re: i cleaned a mold contaminated live culture and saved it. (might have useful applications) [Re: maddchef]
    #21861917 - 06/26/15 10:30 PM (8 years, 6 months ago)

Chances are they both survived. Peroxide just harms molds.


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Edited by Trusted cuItivator (06/26/15 10:31 PM)


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Invisiblemaddchef
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Re: i cleaned a mold contaminated live culture and saved it. (might have useful applications) [Re: bodhisatta]
    #21862088 - 06/26/15 11:14 PM (8 years, 6 months ago)

Honestly I don't see how either survived 35% h2o2


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Offlinerxb
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Re: i cleaned a mold contaminated live culture and saved it. (might have useful applications) [Re: maddchef]
    #21862732 - 06/27/15 05:34 AM (8 years, 6 months ago)

Quote:

maddchef said:
Honestly I don't see how either survived 35% h2o2




:this:

it was a live culture a colonized grain was dropped into a jar or water with honey and left to colonize, then mold was cultured and dropped in.

at this point i see no mold. at the very least it has given something i could pull enough of a clean sample out of to do agar work.

however i think we dont really CARE about that, where it may be useful is cleaning up contaminants during fruiting...when we cant just make another plate or avoid the mold.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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InvisiblebodhisattaMDiscordReddit
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Re: i cleaned a mold contaminated live culture and saved it. (might have useful applications) [Re: rxb]
    #21862886 - 06/27/15 07:11 AM (8 years, 6 months ago)

Yea if anything survived that's astonishing. You should see what 26% does to a human hand.


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Offlinedrake89
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Re: i cleaned a mold contaminated live culture and saved it. (might have useful applications) [Re: bodhisatta]
    #21863118 - 06/27/15 08:31 AM (8 years, 6 months ago)

Where do you get 35% peroxide ? ^_^


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InvisiblebodhisattaMDiscordReddit
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Re: i cleaned a mold contaminated live culture and saved it. (might have useful applications) [Re: drake89]
    #21863222 - 06/27/15 08:55 AM (8 years, 6 months ago)

You can freeze distill I believe.


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Offlinerxb
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Re: i cleaned a mold contaminated live culture and saved it. (might have useful applications) [Re: drake89]
    #21863381 - 06/27/15 09:36 AM (8 years, 6 months ago)

Quote:

drake89 said:
Where do you get 35% peroxide ? ^_^




theres a local store which sells food grade 35% HP for $25 per half gal.

i've also seen it online more expensive..the stuff in the brown bottles is %3 and it has other things in it.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Offlineblackout
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Re: i cleaned a mold contaminated live culture and saved it. (might have useful applications) [Re: drake89]
    #21865013 - 06/27/15 04:38 PM (8 years, 6 months ago)

Quote:

drake89 said:
Where do you get 35% peroxide ? ^_^



hydroponics shops should have it.

I am also shocked 35% did not destroy it. The Rush Wayne guides use peroxide at 0.15%.


Edited by blackout (06/27/15 04:46 PM)


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InvisibleToadstool5
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Re: i cleaned a mold contaminated live culture and saved it. (might have useful applications) [Re: blackout]
    #21913090 - 07/08/15 01:28 AM (8 years, 6 months ago)

I think the peroxidases of mold are much more limited in their ability to donate hydronium ions from the N-H bonds located on the chitosan due to differences in density and availability.

Hydrogen Peroxide does most of its damage in the plasma if i remember correctly. Molds' DNA is floating around the plasma whereas macrofungi have a nucleus protecting the DNA and separating it from the plasma.

Regardless, oxidative damage to the DNA is greater in mold and because mold reproduces through mitosis, if the parent cell is corrupt everything falls apart. Sexual reproduction allows for greater protection of the chromosomes because each half is protected by a different cell.

I just can't believe it could handle such a large concentration! Thats insane :shrug:


--------------------
If you do not know where the mushroom products you are consuming are grown, think twice before eating them. :badshroom:
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Offlinerxb
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Re: i cleaned a mold contaminated live culture and saved it. (might have useful applications) [Re: Toadstool5]
    #21913819 - 07/08/15 07:59 AM (8 years, 6 months ago)

theres still nothing blue in the jar, i wouldnt call it thriving but everything thats in the jar is fluffy or agar.

i would take a picture, but it would be hard to see anything noteworthy, the glass distorts everything and the agar is in a piled at the bottom of the jar,

some myc on agar stuck to the side of the jar (barely) has clearly grown, not fast, but in my opinion i could at this point make some LI/lc and transfer it. i'm going to wait a bit before i do and see if it gets stronger.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Offlinerxb
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Re: i cleaned a mold contaminated live culture and saved it. (might have useful applications) [Re: rxb]
    #21913861 - 07/08/15 08:13 AM (8 years, 6 months ago)

i think the food grade probably has applications in lower concentrations.

HP from the drugstore has varius things in it

Quote:

Most commercial grades of H2O2 contain chelants and sequestrants which minimize its decomposition under normal storage and handling conditions.

The types of stabilizers used in H2O2 vary between producers and product grades. Common stabilizers include:

Colloidal stannate and sodium pyrophosphate (present at 25 - 250 mg/L) are traditional mainstays.
Organophosphonates (e.g., Monsanto's Dequest products) are increasingly common.
Nitrate (for pH adjustment and corrosion inhibition) and phosphoric acid (for pH adjustment) also are used.
Colloidal silicate is used to sequester metals and thereby minimize H2O2 decomposition in certain applications that depend on the bleaching ability of H2O2 in alkali.




which may negatively effect the myc's ability to thrive.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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InvisibleToadstool5
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Re: i cleaned a mold contaminated live culture and saved it. (might have useful applications) [Re: rxb]
    #21917101 - 07/08/15 09:23 PM (8 years, 6 months ago)

Quote:

phosphoric acid




Its a protonogenic sanitizer, used to clean keg lines and brewing equipment. It also causes oxidative damage to the hyphae and i suspect it buffers the medium making harder for the cell wall to adsorb and release ions.

Quote:

Organophosphonates




Sounds like a substituted organophosphate nerve toxin a.k.a. RoundUp™


--------------------
If you do not know where the mushroom products you are consuming are grown, think twice before eating them. :badshroom:
- Paul Stamets

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Offlinebryanbzl
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Re: i cleaned a mold contaminated live culture and saved it. (might have useful applications) [Re: rxb]
    #22093122 - 08/14/15 11:51 PM (8 years, 5 months ago)

Depends on the contaminant.

If it was a mold that produces spores like trich, then I would assume that because it was at the end of its life cycle and produced spores (green), the hydrogen peroxide killed the spores which are the only way the mold could keep living from that point on. Any mycelium would be at the end of its life and would die back on its own leaving the spores to continue the cycle. That's the best I could come up with after coming to the same conclusion with bleach. I have given hydrogen peroxide the boot a long time ago - doesn't cut it with thriving weed mycelium.


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Offlinerxb
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Re: i cleaned a mold contaminated live culture and saved it. (might have useful applications) [Re: bryanbzl]
    #22093555 - 08/15/15 05:05 AM (8 years, 5 months ago)

Quote:

bryanbzl said:
Depends on the contaminant.

If it was a mold that produces spores like trich, then I would assume that because it was at the end of its life cycle and produced spores (green), the hydrogen peroxide killed the spores which are the only way the mold could keep living from that point on. Any mycelium would be at the end of its life and would die back on its own leaving the spores to continue the cycle. That's the best I could come up with after coming to the same conclusion with bleach. I have given hydrogen peroxide the boot a long time ago - doesn't cut it with thriving weed mycelium.




wait, you said hydrogen peroxide, but you didnt mention the concentration you used which makes your input useless.

please advise, what concentration of hydrogen peroxide did you use?


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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OfflineKizzle
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Re: i cleaned a mold contaminated live culture and saved it. (might have useful applications) [Re: maddchef]
    #22099842 - 08/16/15 06:19 PM (8 years, 5 months ago)

Quote:

agar seems to be colonizing with healthy non-mold myc



Sounds like you're jumping to conclusions. Let us know if you actually get some pins growing from it.

Quote:

maddchef said:
Honestly I don't see how either survived 35% h2o2



Peroxide reacts with other organic molecules which are abundant in the agar. Any mycelium beneath the surface is shielded from it's effects to a degree. Also the absorption of the peroxide into the agar happens over a period of time so even if it does reach them intact aerobic organisms have more time to break it down before it reaches dangerous levels.


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