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Angus
Trailer Park Supervisor



Registered: 03/06/08
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Last seen: 1 year, 10 months
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Mycellium syringe preperation question
#21859070 - 06/26/15 09:18 AM (8 years, 7 months ago) |
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I usually use a multispore inoculation on a few jars, then use those jars to make mycellium syringes.
The way I have been doing this is to fill a few 60cc syringes with water, sterilize, cool, and inject into my multispore jars. Then agitate and aspirate the now myc-laden water back into the syringes.
This has been working well enough, but I was wondering if (based on anyone's experience or best logic) it would be possible to sterilize a jar full of water the same as I would a jar of grain spawn, then use fresh-from-the-sterile-packaging syringes and needle to aspirate water from the jar as needed? After that, I would continue as usual by shooting this water into a colonized jar, agitating, and using the aspirated myc-water as inoculant.
Has anyone done this before? Any glaring omissions or oversights to the idea that I might be blind to?
Thanks in advance for any advice! I hope my post made sense
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blojo02184
Big Red



Registered: 05/15/13
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Loc: Maine
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Re: Mycellium syringe preperation question [Re: Angus]
#21859186 - 06/26/15 09:48 AM (8 years, 7 months ago) |
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I dont see why it wouldnt work... I also dont know why you havnt done a lc...
Make the lc, and use your grain lc to inoculate it.
Never messed around with any of this stuff, so someone else will chime in im sure
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Ncogneato
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Re: Mycellium syringe preperation question [Re: blojo02184]
#21859224 - 06/26/15 09:54 AM (8 years, 7 months ago) |
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Yes. That works just fine.
-------------------- Psilocybin.........the poor man's Cancun.
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coffeehead
Perpetual Learner

Registered: 01/15/13
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Re: Mycellium syringe preperation question [Re: Angus]
#21859233 - 06/26/15 09:55 AM (8 years, 7 months ago) |
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I've used many "fresh-from-the-sterile-packaging syringes and needle" with zero contamination problems. Just be sure to use at least a 29 mm SFD (or equivalent) on the water jar or you'll have difficulties sucking the water from the jar because of the vacuum created. Everything should work fine for you.
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Achillita
Back to the basics



Registered: 05/26/14
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Re: Mycellium syringe preperation question [Re: blojo02184]
#21859236 - 06/26/15 09:56 AM (8 years, 7 months ago) |
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I'm pretty sure this is called Grain Liquid Culture. I've seen someone make GLC, and then let the myc recolonize and do it over. They had to keep turning the jar around because otherwise the excess water would drown some of the mycelium.
But using sterile water from a jar should work completely fine.
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Angus
Trailer Park Supervisor



Registered: 03/06/08
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Re: Mycellium syringe preperation question [Re: blojo02184]
#21859238 - 06/26/15 09:56 AM (8 years, 7 months ago) |
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Quote:
blojo02184 said: I dont see why it wouldnt work... I also dont know why you havnt done a lc...
Make the lc, and use your grain lc to inoculate it.
Never messed around with any of this stuff, so someone else will chime in im sure
Thanks!
I've had bad luck with liquid cultures. Either being contaminated, or me being nervous to use them for fear of contamination. I like using a myc syringe because I can tell that the 'donor' jar is definitely clean. It also keeps things 'fresh', since I'll usually do 14 quarts and two half pints each spawn run. The two half pints being the new 'donor' jars. I guess more than anything, it's just worked for so long so I'm hesitant to try and fix something that isn't broke.
I'm sure LC techniques have advanced since the last time I tried to make one..so perhaps I'll go search for a 'modern' tek that seems to have good success.
Thank again for the reply
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Ncogneato said: Yes. That works just fine.
Excellent. Thank you!
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coffeehead said: Just be sure to use at least a 29 mm SFD (or equivalent) on the water jar or you'll have difficulties sucking the water from the jar because of the vacuum created.
Thanks for the response. I actually hadn't put much thought into what type of filter to use for the water jar. Do you think using a simple wad of polyfill like with spawn jars would be too much of a risk? Seems like it shouldn't be anymore so than any grain jar inoculation?
Quote:
Achillita said: I'm pretty sure this is called Grain Liquid Culture. I've seen someone make GLC, and then let the myc recolonize and do it over. They had to keep turning the jar around because otherwise the excess water would drown some of the mycelium.
But using sterile water from a jar should work completely fine.
Hey buddy, thanks for the reply! That term sounds kind of familiar haha. I've had jars re-colonize, but never felt comfortable using them for a second round. Actually, I usually open the 'donor' jar after the deed is done and give it a good sniff to make sure nothing smells off.
Thanks for verifying what others were saying..I feel pretty good about trying this out now.
Thanks everyone!
Edited by Angus (06/26/15 10:03 AM)
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Achillita
Back to the basics



Registered: 05/26/14
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Re: Mycellium syringe preperation question [Re: Angus]
#21859247 - 06/26/15 09:58 AM (8 years, 7 months ago) |
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If you use clean agar wedges to inoculate an LC, then there should be very little problems with it. Spores straight to LC is pretty easily contamed.
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Angus
Trailer Park Supervisor



Registered: 03/06/08
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Re: Mycellium syringe preperation question [Re: Achillita]
#21859279 - 06/26/15 10:07 AM (8 years, 7 months ago) |
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Quote:
Achillita said: If you use clean agar wedges to inoculate an LC, then there should be very little problems with it. Spores straight to LC is pretty easily contamed.
I'm just getting back into the hobby after a pretty good hiatus. Most of my equipment is lost or given away so I'm not set up to be doing any agar play (work) at the moment. My previous attempts at an LC were from multispore, though, so that could certainly have contributed to their failures. I'm curious, now, if myc water to sugar solution might have better success at spawning a healthy LC..
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Achillita
Back to the basics



Registered: 05/26/14
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Re: Mycellium syringe preperation question [Re: Angus]
#21859318 - 06/26/15 10:16 AM (8 years, 7 months ago) |
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It should be fine as long as everything is sterile. I haven't messed around with GLC, but I've read it's faster than LC usually.
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