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OfflineMachiavelliavore
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Re: Can you save mycelium for future use in grain jars [Re: spacechildo]
    #21861135 - 06/26/15 06:13 PM (8 years, 7 months ago)

Nope but the point is more rapid expansion yes?  It takes well over a month to expand a clone to an amount that could inoculate multiple quart jars with grain petris.  G2Ging with a master culture on grain will be quite slow unless you expend a considerable quantity of grain each time you use it, meaning you'll run out of master culture in short order.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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InvisibleMad Season
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Re: Can you save mycelium for future use in grain jars [Re: Machiavelliavore]
    #21861158 - 06/26/15 06:21 PM (8 years, 7 months ago)

Tbh I don't trust LC from spore syringe. I feel agar and LC go hand in hand. They're the best combination for every aspect. Inoculate LC with a clean wedge, draw in syringe and test its cleanliness on a few agar dishes. Then run TONS of grain jars from 1 lc. You can store it in the fridge. Make a massive amount of inoculant and agar dishes. Imo lc is the shit.


--------------------
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Offlinespacechildo
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Re: Can you save mycelium for future use in grain jars [Re: Mad Season]
    #21861168 - 06/26/15 06:23 PM (8 years, 7 months ago)

yeah its more a replacement for g2g than agar. both ways can give you way more spawn than most people have jars to do in a very short time.


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InvisibleMad Season
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Re: Can you save mycelium for future use in grain jars [Re: spacechildo]
    #21861176 - 06/26/15 06:26 PM (8 years, 7 months ago)

:whathesaid: everything you do should get started on agar. Even LC. At least if you want to do it right.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineMachiavelliavore
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Re: Can you save mycelium for future use in grain jars [Re: Mad Season]
    #21861344 - 06/26/15 07:22 PM (8 years, 7 months ago)

The more volatile the medium, the more it makes sense to start from the cleanest source.

However for someone without agar, I think I would trust a vendor syringe that has proven itself to be clean or a nice colonized grain over a spore print that's passed through open air.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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InvisibleMad Season
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Re: Can you save mycelium for future use in grain jars [Re: Machiavelliavore]
    #21861377 - 06/26/15 07:35 PM (8 years, 7 months ago)

Grain is a way different story than LC. Bacteria and molds grow WAY better in LC. Even a small amount of contamination can fuck it all up. I shoot spore syringes on agar and can't believe the amount of bacteria or strange stuff I see. Albeit most are clean. It isn't uncommon to see some monstrosities.

As for agar go to the top where there's sponsors flashing in your face. It'll say please support our sponsors. Do a little shopping there. A quick search found a bottle of pre mixed agar for under 15$. Hint don't try the places with spores.

You can also buy agar online in powder form and buy your own malt. Lol. Where there's a will there's a way :smile:. I agree though you can just inoculate jars and get by with g2gs or something. However if you live in a bad environment like me you need agar. It was my saving grace and I'd have quit long ago in frustration without it.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (06/26/15 07:36 PM)


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OfflineMachiavelliavore
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Re: Can you save mycelium for future use in grain jars [Re: Mad Season]
    #21861414 - 06/26/15 07:50 PM (8 years, 7 months ago)

It's not quite worth spending for me yet (not to make this thread about me.)  I think I will try tearing some rhizo mycellium that are prickling up off the top of a grain petri with tweezers and dumping them into LC and see what happens, then test that LC on grain petris for purity.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Offlinespacechildo
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Re: Can you save mycelium for future use in grain jars [Re: Machiavelliavore]
    #21861417 - 06/26/15 07:51 PM (8 years, 7 months ago)

what's a grain petri?


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Invisibleeatyualive
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Re: Can you save mycelium for future use in grain jars [Re: spacechildo]
    #21861421 - 06/26/15 07:52 PM (8 years, 7 months ago)

grain water agar plate is what it sounds like. or hes putting a piece of grain on an agar plate.
g2a.


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OfflineMachiavelliavore
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Re: Can you save mycelium for future use in grain jars [Re: spacechildo]
    #21861427 - 06/26/15 07:54 PM (8 years, 7 months ago)

Just 1/8th of a cup of grains in a half pint.  I've been able to get a lot of clean clones and clean up some contamination on them with inteligent transfers.  Pretty handy.  Got the idea from violet, but she doesn't use them as a ghetto agar (check for contams, clone, transfer,) only a microscale culture testgrow.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


Edited by Machiavelliavore (06/26/15 07:55 PM)


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Invisibleeatyualive
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Re: Can you save mycelium for future use in grain jars [Re: eatyualive]
    #21861430 - 06/26/15 07:55 PM (8 years, 7 months ago)

ok op, so i regularly store masters in the fridge of colonized grain jars for future use. ill take them out when ready and g2g. but, the grain jars do have a shelf life. if you see metabolite(yellow or liquid looking water pooling in the jar) or off colors when you take it out of the fridge. don't use it. you want healthy white mycelia.

ive had success numerous times using jars up to 1 year old. but this also is dependent on culture/genetics.

the most id suggest storing them in the fridge is 6 months.


--------------------
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:flame::chief:JOIN THE POW WOW:chief::flame:


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OfflineMachiavelliavore
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Re: Can you save mycelium for future use in grain jars [Re: eatyualive]
    #21861437 - 06/26/15 07:57 PM (8 years, 7 months ago)

Do you perpetuate the same culture by expanding it every 6 months to get new mycellium to refridgerate?


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Offlinespacechildo
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Re: Can you save mycelium for future use in grain jars [Re: eatyualive]
    #21861439 - 06/26/15 07:58 PM (8 years, 7 months ago)

Quote:

eatyualive said:
grain water agar plate is what it sounds like. or hes putting a piece of grain on an agar plate.
g2a.




he just said he didn't have money for agar... :confused:

ninja; oh OK, a half pint jar of grains. not a petri dish :super:


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Invisibleeatyualive
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Re: Can you save mycelium for future use in grain jars [Re: Machiavelliavore]
    #21861450 - 06/26/15 08:02 PM (8 years, 7 months ago)

Quote:

Machiavelliavore said:
Do you perpetuate the same culture by expanding it every 6 months to get new mycellium to refridgerate?



no, once that grain jar is used, i start again from master culture. from a plate/liquid culture ect.
only first transfer masters are stored in the fridge. never anything older than that.


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OfflineMachiavelliavore
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Re: Can you save mycelium for future use in grain jars [Re: eatyualive]
    #21861533 - 06/26/15 08:28 PM (8 years, 7 months ago)

Quote:

spacechildo said:
Quote:

eatyualive said:
grain water agar plate is what it sounds like. or hes putting a piece of grain on an agar plate.
g2a.




he just said he didn't have money for agar... :confused:

ninja; oh OK, a half pint jar of grains. not a petri dish :super:





I call it a grain petri because that's what violet calls essentially the same thing.  Very thin, maybe 3 grains thick, so it's easy to see what's growing, and it doesn't require lots of time or a shake (which would make transfering away from contams very hard) to get completely colonized.

The issue I have with it is that I end up with 1/8 cup of grains as my master culture.  Usually I have to expand that to another grain petri, then G2G that into a quart of grains, then g2g that to more grains.  The whole process is rather lengthy, which is why I want to try making an LC from the master petri.

Quote:

eatyualive said:
Quote:

Machiavelliavore said:
Do you perpetuate the same culture by expanding it every 6 months to get new mycellium to refridgerate?



no, once that grain jar is used, i start again from master culture. from a plate/liquid culture ect.
only first transfer masters are stored in the fridge. never anything older than that.



Do plates or LC's have greater longevity in the frige?  I was under the impression that they didn't, though I hadn't found a satisfactory search result or bothered to ask yet.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Invisibleeatyualive
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Re: Can you save mycelium for future use in grain jars [Re: Machiavelliavore]
    #21861537 - 06/26/15 08:29 PM (8 years, 7 months ago)

ive stored a liquid culture syringe from 2007 of oysters in the fridge.

here is a thread of that same culture. since 2007 ive used about a total of 1cc of solution. blues and pearls both from 2007.

http://www.shroomery.org/forums/showflat.php/Number/21744280/fpart/1/vc/1

here is a recent tub grow of the same pearl culture. although the fae was shit.



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InvisiblefahtsterM
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Re: Can you save mycelium for future use in grain jars [Re: eatyualive]
    #21865469 - 06/27/15 06:37 PM (8 years, 7 months ago)

you can dry them out too for long storage... agar is the surefire way, but there are others... I didn't have a flow hood and didn't trust SAB's enough with my masters so I devised this:

http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6590986/an/0/page/1

again, agar is your best bet, but maybe the above link will help in other ways.

faht


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