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OfflineJohnDoeKENTUCKY
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Can you save mycelium for future use in grain jars
    #21857855 - 06/25/15 11:07 PM (8 years, 7 months ago)

Hi, I am using rye seed as a bulk substrate and I have already bought 2 golden t syringes and basically my question is when I am ready to inoculate more rye berry jars do I have to buy more syringes or can I just do a G2G transfer from an old inoculated jar I had from say 2 months back that I put in the fridge?

I am aware that I can make a spore print but I would like to make it simple as possible and stick to G2G transfers for my bulk substrate grows.

Thanks and sorry if this question sounds stupid or repetitive I haven't found any threads exactly like this on the forum.


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OfflineSizlChest
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Re: Can you save mycelium for future use in grain jars [Re: JohnDoeKENTUCKY]
    #21857911 - 06/25/15 11:19 PM (8 years, 7 months ago)

:takingnotes:


--------------------
PrimalSoup's Tea Tek

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"Hell, shrooms have blown up from under me and kicked my ass on 2 grams once."
"I think ill eat some shrooms right about now, and ill continue to until it doesnt feel like the right thing to do."


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InvisibleBuck513

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Re: Can you save mycelium for future use in grain jars [Re: SizlChest]
    #21857922 - 06/25/15 11:22 PM (8 years, 7 months ago)

Uhh..just get into agar:shrug:

Keep a mini fridge full of dozens of cultures, just grab one that you'd like to use at that time, knock up a jar, and expand:shrug:


--------------------
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InvisibleMad Season
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Re: Can you save mycelium for future use in grain jars [Re: SizlChest]
    #21857938 - 06/25/15 11:28 PM (8 years, 7 months ago)

Technically if the jar is clean, then you can easily g2g it. However ime, clean grain jars are hard to come by. Perfectly fine for bulk, but I'd never use a jar with even a hint of bacteria for g2g. Basically a few pics of the jar would determine if it's good for g2g. Otherwise I'd just go back to spore.

If you're open to trying agar, you can take a few grains and put it on agar before spawning to bulk. Also you should think about putting your syringes onto agar too.


--------------------
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Edited by Mad Season (06/25/15 11:29 PM)


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OfflineGreen Bastard
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Re: Can you save mycelium for future use in grain jars [Re: JohnDoeKENTUCKY]
    #21857999 - 06/25/15 11:42 PM (8 years, 7 months ago)

Quote:

JohnDoeKENTUCKY said:
Hi, I am using rye seed as a bulk substrate....




Hope you're not actually planning to use rye as your bulk sub.
Probably just a terminology thing, eh?


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OfflineMachiavelliavore
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Re: Can you save mycelium for future use in grain jars [Re: Green Bastard]
    #21858527 - 06/26/15 04:03 AM (8 years, 7 months ago)

As stated, if the jar is clean, you can definitely G2G with it.

I would advise you to make a small jar of grain to G2G into.  Observe it for contamination, then use that jar to G2G a meaningful quantity.  You could also make Liquid Culture and drop a good looking single colonized grain in AFAIK, or a drop from the spore syringe.  Further liquid cultures could be inoculated with the original.  Again I'd advise you to test it on a small quantity of grain to make sure it is what you think it is.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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OfflineJohnDoeKENTUCKY
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Re: Can you save mycelium for future use in grain jars [Re: Machiavelliavore]
    #21859134 - 06/26/15 09:35 AM (8 years, 7 months ago)

I understand what you are saying but what I don't understand is the point in using LC's whatsoever. They seem pretty useless if you can preserve a colonized jar are rye in the refrigerator, I guess because less contams


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OfflineJohnDoeKENTUCKY
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Re: Can you save mycelium for future use in grain jars [Re: Green Bastard]
    #21859142 - 06/26/15 09:39 AM (8 years, 7 months ago)

Quote:

Green Bastard said:
Quote:

JohnDoeKENTUCKY said:
Hi, I am using rye seed as a bulk substrate....




Hope you're not actually planning to use rye as your bulk sub.
Probably just a terminology thing, eh?




I am using rye berries only for my bulk substrate is this bad? I have already pasteurized much coir and verm for casing..  Should I use rye as spawn and spawn it in to hpoo or straw? I feel that using rye alone (being cased) would be easiest and less contaminate prone then spawning in to hpoo.
-john


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InvisibleSteveRogers
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Re: Can you save mycelium for future use in grain jars [Re: JohnDoeKENTUCKY]
    #21859167 - 06/26/15 09:44 AM (8 years, 7 months ago)

Quote:

JohnDoeKENTUCKY said:
Quote:

Green Bastard said:
Quote:

JohnDoeKENTUCKY said:
Hi, I am using rye seed as a bulk substrate....




Hope you're not actually planning to use rye as your bulk sub.
Probably just a terminology thing, eh?




I am using rye berries only for my bulk substrate is this bad? I have already pasteurized much coir and verm for casing..  Should I use rye as spawn and spawn it in to hpoo or straw? I feel that using rye alone (being cased) would be easiest and less contaminate prone then spawning in to hpoo.
-john




Yes it's bad. Bulk substrate is more along the lines of straw/manure/coir. Do not pre pasteurize your bulk substrate. prepare it when you have CLEAN spawn ready to transfer.

Good luck to you


--------------------
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Edited by SteveRogers (06/26/15 09:46 AM)


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InvisibleMad Season
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Re: Can you save mycelium for future use in grain jars [Re: JohnDoeKENTUCKY]
    #21859175 - 06/26/15 09:46 AM (8 years, 7 months ago)

Coir and verm can and will work the same way as hpoo and straw. Although it can be used for casings, it is technically these days a bulk substrate. Basically you should spawn the rye into a tub of coir verm and gypsum (cvg)


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineJohnDoeKENTUCKY
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Re: Can you save mycelium for future use in grain jars [Re: Mad Season]
    #21859479 - 06/26/15 10:57 AM (8 years, 7 months ago)

So I should take the colonized grain and mix it with the coir/verm and mix it real good or case it with the coir and verm?
-john


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InvisibleMad Season
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Re: Can you save mycelium for future use in grain jars [Re: JohnDoeKENTUCKY]
    #21859487 - 06/26/15 10:59 AM (8 years, 7 months ago)

I'd do option one if it were me. Maximize the yield as much as I can.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Offlinespacechildo
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Re: Can you save mycelium for future use in grain jars [Re: Mad Season]
    #21859526 - 06/26/15 11:06 AM (8 years, 7 months ago)

do both! :super:

even if you're gonna g2g from master in the fridge you're gonna have to start over at some point.
you cant g2g a jar forever or keep g2g'ing the same culture forever.

taking prints is easy, foil, cap, cover, bam!


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OfflineZorroro
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Re: Can you save mycelium for future use in grain jars *DELETED* [Re: Buck513]
    #21859563 - 06/26/15 11:14 AM (8 years, 7 months ago)

Post deleted by Zorroro

Reason for deletion: .



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InvisibleBuck513

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Re: Can you save mycelium for future use in grain jars [Re: Zorroro]
    #21859566 - 06/26/15 11:15 AM (8 years, 7 months ago)

Months if kept in a clean ziploc bag.

Slants store for longer


--------------------
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Enter the Ban Lottery


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OfflineShamanic Scientist
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Re: Can you save mycelium for future use in grain jars [Re: JohnDoeKENTUCKY]
    #21859781 - 06/26/15 12:14 PM (8 years, 7 months ago)

I have kept colonized rye grain in the fridge for 2-3 months before, and subsequently sued it for g2g. It worked just fine. Might take a couple days or so before the mycelia "wake up" and you start to see growth on the new grain.


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InvisibleBuck513

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Re: Can you save mycelium for future use in grain jars [Re: Shamanic Scientist]
    #21859797 - 06/26/15 12:19 PM (8 years, 7 months ago)

Storing cultures is the way to go OP.

You can have clones of different varieties and just pick one to work with.

You could keep dozens of cultures, but have fun keeping dozens of jars that you're trying to expand lol.

Plus you shouldn't expand the same culture via g2g more than a few times


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Enter the Ban Lottery


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InvisibleMad Season
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Re: Can you save mycelium for future use in grain jars [Re: Buck513]
    #21859831 - 06/26/15 12:27 PM (8 years, 7 months ago)

:whathesaid: they say max 3 g2g transfers, however you can do 20 transfers if you so desire on agar. They say g2gs are best for expanding your myc, I'd have to disagree. After 20 transfers you could easily multiply it up to thousands or tens of thousands of dishes (not realistic) and could inoculate tens of thousands to a few hundred thousand grain jars. I'd like to see you do that with g2g. After 3 transfers 1 jar would make 1000 grain jars.

It might take a little longer, but it's guaranteed clean inoculant.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (06/26/15 12:29 PM)


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OfflineMachiavelliavore
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Re: Can you save mycelium for future use in grain jars [Re: JohnDoeKENTUCKY]
    #21861094 - 06/26/15 06:02 PM (8 years, 7 months ago)

Quote:

JohnDoeKENTUCKY said:
I understand what you are saying but what I don't understand is the point in using LC's whatsoever. They seem pretty useless if you can preserve a colonized jar are rye in the refrigerator, I guess because less contams




As I see it, the point of LC is that you can quickly expand a master culture or small quantity of spore syringe into a useful amount of inoculant.  The other option for this is agar.

If you want to inoculate 6 jars with a tiny piece of clone tissue, or 1ml left in your spore syringe, it would take weeks if not well over a month to colonize and expand out into a sufficient amount of grain to inoculate all those jars.  It would be much faster with an LC. 

Also, if you had a master culture rye jar, instead of needing to use half a cup from it per jar, you could take a single grain, make LC, and noc 6 jars.

From what you've said it would make the most sense to make an LC with the last of your spore syringe if you have any left.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Offlinespacechildo
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Re: Can you save mycelium for future use in grain jars [Re: Machiavelliavore]
    #21861114 - 06/26/15 06:07 PM (8 years, 7 months ago)

you've never done either of those methods have ya? :lol:

there was just too much in there to try and correct or guide :tongue2:


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