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OfflineBLINKfan420
Jedi
I'm a teapot


Registered: 07/06/12
Posts: 1,078
Last seen: 5 months, 1 day
Rye Jars Not Colonizing
    #21857617 - 06/25/15 10:20 PM (8 years, 7 months ago)

Hello all. I have some rye berry jars showing no growth after over a week and no contams.

Here are the deets:

I made a batch of 6 qt sized rye berry jars.  Soaked for 24 hrs with a teaspoon of gypsum, boiled for 15 min (until a couple grains had burst). Then spread out on cookie cooling sheets until they passed the "roll test" on a piece of TP, showing no wet marks. Finally, PC'ed at 15psi for 90 min and left over night to cool.

Heres the issue:
I used 2 different MS syringes that I made myself from prints.  3 jars knocked with syringe #1 and 3 jars knocked with syringe #2.  The jars knocked with syringe #1 show ZERO growth, no contams or funny smells, nothing.  The 3 jars knocked with syringe #2 are all showing signs of healthy mic.

This is the second time this problem has occurred with the MS syringe.  Last time I double knocked them without thinking and they all colonized just fine.  But this time I figured I'd ask around.

I've heard double inoculations are frowned greatly upon.  People say, "if it didn't grow the first time why would it the second?" In my case it's obviously the syringe that must not be good... right? Most are going to say just start over, but if I have a "dead" syringe, if you will, what is the harm?


BLiNK


--------------------



All my pictures are drawn from imagination.  I don't even know what a mushroom is.


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Offlineshroomyaxn
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Registered: 11/13/11
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Re: Rye Jars Not Colonizing [Re: BLINKfan420]
    #21857683 - 06/25/15 10:34 PM (8 years, 7 months ago)

What temperature are the jars colonizing at?
Also, do the rye jars look wet?


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InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: Rye Jars Not Colonizing [Re: shroomyaxn]
    #21858047 - 06/25/15 11:55 PM (8 years, 7 months ago)

What kind of filter are you using?
And just because you don't SEE growth, doesn't mean nothing is happening


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Rye Jars Not Colonizing [Re: Buck513]
    #21858435 - 06/26/15 03:02 AM (8 years, 7 months ago)

If you want answers, suggestions or any other advice, pictures will be your best friend.


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OfflineBLINKfan420
Jedi
I'm a teapot


Registered: 07/06/12
Posts: 1,078
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Re: Rye Jars Not Colonizing [Re: insanemike]
    #21858491 - 06/26/15 03:39 AM (8 years, 7 months ago)

The jars are at a pretty consistent 75°F sitting on a shelf.

The filter is a Tyvek disc.  The lid has four tiny holes drilled in with a 3/32 bit.

The rye does not look wet to me at all. I even thought that this batch turned out a litte bit dryer than I expected, I added a too much gypsum (which is a problem of mine.. I need to learn to measure that stuff before I toss it in there) so there is some sandy looking stuff..

Here are some pics, first three are the jars in question.



These are the three that have growth..



BLiNK


Edited by BLINKfan420 (06/26/15 03:45 AM)


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InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: Rye Jars Not Colonizing [Re: BLINKfan420]
    #21858495 - 06/26/15 03:41 AM (8 years, 7 months ago)

You're growing mold.


But I'm not sure if its the kind you want.


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


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OfflineBLINKfan420
Jedi
I'm a teapot


Registered: 07/06/12
Posts: 1,078
Last seen: 5 months, 1 day
Re: Rye Jars Not Colonizing [Re: Buck513]
    #21858513 - 06/26/15 03:53 AM (8 years, 7 months ago)

This is mold??? Maybe I need a better camera..



BLiNK


--------------------



All my pictures are drawn from imagination.  I don't even know what a mushroom is.


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Rye Jars Not Colonizing [Re: BLINKfan420]
    #21858554 - 06/26/15 04:31 AM (8 years, 7 months ago)

From my vantage point, the bottom of them jars are way too wispy to be cube myc. It may be infected with cobweb mold. The myc toward the top looks okay though.


Edited by insanemike (06/26/15 04:32 AM)


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OfflineBLINKfan420
Jedi
I'm a teapot


Registered: 07/06/12
Posts: 1,078
Last seen: 5 months, 1 day
Re: Rye Jars Not Colonizing [Re: insanemike]
    #21860821 - 06/26/15 04:28 PM (8 years, 7 months ago)

This isn't my first time with rye and it doesn't look like anything suspicious to me. :shrug:  I feel like the camera doesn't do it justice. I guess time will tell. 

And good thing I'm about to start agar.. then I can clean the plate up and get rid of the contams right?

BLiNK


--------------------



All my pictures are drawn from imagination.  I don't even know what a mushroom is.


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OfflineSksoul
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Registered: 10/31/14
Posts: 397
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Re: Rye Jars Not Colonizing [Re: insanemike]
    #21860955 - 06/26/15 05:23 PM (8 years, 7 months ago)

Quote:

BLINKfan420 said:
Here are some pics, first three are the jars in question.



These are the three that have growth..



BLiNK






Quote:

Buck513 said:
You're growing mold.


But I'm not sure if its the kind you want.





Quote:

insanemike said:
From my vantage point, the bottom of them jars are way too wispy to be cube myc. It may be infected with cobweb mold. The myc toward the top looks okay though.






I think its just a bad picture. The bottles are white on the bottom from the pc run. Maybe sat up to that height in water.


--------------------
Like all great travellers, I have seen more than I remember, and remember more than I have seen.


Edited by Sksoul (06/26/15 06:15 PM)


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InvisibleMad Season
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Registered: 09/16/12
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Re: Rye Jars Not Colonizing [Re: Sksoul]
    #21861020 - 06/26/15 05:44 PM (8 years, 7 months ago)

Honestly it just looks like the reflection from the white table/surface. Put it on a black surface and take more pics just to be sure.

EDIT: I think your last set of pics look decent :smile:


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How to shroomery like a pro! (Seriously, everyone read this!)
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Edited by Mad Season (06/26/15 05:45 PM)


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Offlinespacechildo
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Re: Rye Jars Not Colonizing [Re: Mad Season]
    #21861145 - 06/26/15 06:17 PM (8 years, 7 months ago)

million $ question, how long has it been? answered in OP, 1 week,
my advice smoke a bowl and wait another week!

I too thought the white stuff was just light reflection and OP would've commented on it if something was there..


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OfflineBLINKfan420
Jedi
I'm a teapot


Registered: 07/06/12
Posts: 1,078
Last seen: 5 months, 1 day
Re: Rye Jars Not Colonizing [Re: spacechildo]
    #21861241 - 06/26/15 06:47 PM (8 years, 7 months ago)

At this point it isn't spreading quick enough to be cobweb.  There is also rhizomorphic pure white growth in many areas.  I'm just bummed the other 3 didn't take off... it makes me really think the syringe is weak or bad.  But I don't wanna toss them or do anything until I'm sure of it.


BLiNK


--------------------



All my pictures are drawn from imagination.  I don't even know what a mushroom is.


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InvisibleMad Season
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Re: Rye Jars Not Colonizing [Re: spacechildo]
    #21861263 - 06/26/15 06:54 PM (8 years, 7 months ago)

Quote:

spacechildo said:
million $ question, how long has it been? answered in OP, 1 week,
my advice smoke a bowl and wait another week!

I too thought the white stuff was just light reflection and OP would've commented on it if something was there..



Op please listen to this. Anybody who's been in the game for awhile has had the few jars/dishes that didn't show shit for 3 weeks+.

Also the fact it's conveniently on the bottom half of ALL the jars with a white surface under it seriously makes me believe it is just the reflection. You can call me Sally if it isn't.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (06/26/15 06:57 PM)


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Offlinespacechildo
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Re: Rye Jars Not Colonizing [Re: BLINKfan420]
    #21861265 - 06/26/15 06:54 PM (8 years, 7 months ago)

so there is gray stuff covering the bottom half of all your first 3 jars that has no cube myc growth??

if not and it was just miscommunication wait another week at least. spores can take up to 3 weeks to germinate, especially if they are some yrs old.


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OfflineBLINKfan420
Jedi
I'm a teapot


Registered: 07/06/12
Posts: 1,078
Last seen: 5 months, 1 day
Re: Rye Jars Not Colonizing [Re: spacechildo]
    #21861550 - 06/26/15 08:34 PM (8 years, 7 months ago)

To the eye there is no grey growth in the jars.

The spores are about a year old maybe a little older.

Yes, there are most definitely water marks all over the  bottom portion of the jars from the PC.  I used to wipe them off but after a few grows I only wipe off the water marks if it's covering something I really need to see, but for the most part it's pretty transparent to the naked eye. I guess I'll try to raise them up higher in the PC so the water doesn't touch them in the future.

I'll give the jars 3 weeks until I make a decision to toss them or to chance knocking them up twice.  However, after 3 weeks I'm not sure how the water content will be.

It's good to know that no growth in a week is no big deal, it just concerns me since they were all made from the same batch of rye and 3 are good and 3 arent. =/

BLiNK


--------------------



All my pictures are drawn from imagination.  I don't even know what a mushroom is.


Edited by BLINKfan420 (06/26/15 08:39 PM)


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InvisibleMad Season
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Registered: 09/16/12
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Re: Rye Jars Not Colonizing [Re: BLINKfan420]
    #21861563 - 06/26/15 08:41 PM (8 years, 7 months ago)

Are siblings always identical? Genetics are always different :smile: no 2 jars will be the same when using spores. Glad there's no visible grey :wink: I'm sure you'd notice it if it was over the bottom half of all your jars lol. Also happy I'm not Sally haha


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (06/26/15 08:43 PM)


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