Home | Community | Message Board

Mycohaus
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineRescueShroom
Dildont

Registered: 06/09/15
Posts: 56
Loc: Bat Country
Last seen: 7 years, 10 months
Isolating question
    #21857148 - 06/25/15 08:56 PM (8 years, 7 months ago)

I'm planning on moving away from MS and onto agar. First I am wanting to isolate. I have pretty much the teks I am going to follow, but I have some questions. When you start sectoring out and transferring to different plates when do you start fruiting? Do you wait until you get a pure isolate to start? And what do you do with all the plates that you took wedges from that weren't isolates? Just toss them?

I'm planning on starting with a cloned fruit from my current BRF cakes. And isolate from there. I've heard it's easier then starting from MS directly.


Extras: Filter Print Post Top
Offlinerxb
n00b-sabot
 User Gallery


Registered: 08/24/13
Posts: 9,521
Loc: FREE PSYCHONAUTICA
Last seen: 3 hours, 8 minutes
Re: Isolating question [Re: RescueShroom]
    #21857183 - 06/25/15 09:02 PM (8 years, 7 months ago)

1) whats the point of isolation if you dont isolate.

so yes, you wait until you have a single growth covering the whole plate.

2) theres many ways you can do it, you can make a live culture/live inoculate from the plate... or just drop a wedge onto the grains.

yes you can toss the partial plates, you dont need them.

but you CAN use them to isolate OTHER strains and compare good characteristics (like fast growth) and use the ones with traits you like.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


Extras: Filter Print Post Top
OfflineRescueShroom
Dildont

Registered: 06/09/15
Posts: 56
Loc: Bat Country
Last seen: 7 years, 10 months
Re: Isolating question [Re: rxb]
    #21857203 - 06/25/15 09:05 PM (8 years, 7 months ago)

Thanks you are awesome. Is it harder to isolate from a MS syringe then from a cloned fruit? In my mind it would seem to be because how would you know if you have fruiting mycelium or not.


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Isolating question [Re: RescueShroom]
    #21857258 - 06/25/15 09:16 PM (8 years, 7 months ago)

Non fruiting is rare. Clones may be closer to isolates or you may get lucky and a fruit Clone may be an isolated first shot. It's best to just fruit clones. Isolate in your spare time most isolates suck for every 100 isolates maybe a few are worth keeping.


Extras: Filter Print Post Top
Invisiblemaddchef
Vaginal escape artist
 User Gallery


Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Isolating question [Re: bodhisatta]
    #21857736 - 06/25/15 10:44 PM (8 years, 7 months ago)

I gave up finding isolates. I used to always get better results with clones as the genetics seemed complimentary


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: Isolating question [Re: bodhisatta]
    #21858041 - 06/25/15 11:53 PM (8 years, 7 months ago)

Quote:

bodhisatta said:
It's best to just fruit clones. Isolate in your spare time most isolates suck for every 100 isolates maybe a few are worth keeping.



This right here


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 10 hours
Trusted Cultivator
Re: Isolating question [Re: maddchef]
    #21858124 - 06/26/15 12:20 AM (8 years, 7 months ago)

Im with Bodhisatta about making strain isolation, more of a second thought.

When I start with a new print, I put to agar to get clean spawn and do MS grows to search out awesome clones... I do further work with those clones and usually do my grows with those.

But at the same time I will keep transfering from the original culture from spores and isolate.

Once youve made enough transfers that you see obvious sectoring, isolate out a piece of each sector to a blank plate. If your culture no longer shows *any* visible sectoring than youve got an isolate, you will want to put this aside to test.
If it any of the plates still show sectoring you will want to isolate further might take another transfer or two

I try to get 8 to 10 isolates out of a culture to test out. Not all gunna rock out.. Ive been saving the best one out of these to slant and then I start again from spore and do it again.
Eventually youll find something awesome but as Im finding out it take a lot of work and dedicatiom but hey its fun

Its definitly worth it to isolate IMO. But for our purposes if you stabalize a rocking clone you can get the same consistent results


--------------------

Amateur Mycologists United
AMU Q&A


Edited by mushpunx (06/26/15 12:23 AM)


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* might be an advance agar question
( 1 2 all )
fugu 10,917 33 02/05/03 06:45 AM
by fugu
* Penis Envy isolation
( 1 2 3 4 ... 15 16 )
verum subsequentis 21,215 302 01/20/20 07:23 AM
by renr
* Isolating a copelandia cyanescens print TrippinSLIM 1,397 3 12/31/19 08:59 AM
by dionysiusnextdoor
* newbie question- viable spores from dried shrooms? mr crisper 2,659 5 05/14/02 05:18 AM
by ChromeCrow
* G2G transfers
( 1 2 all )
kjb1891 32,782 20 12/19/18 11:45 AM
by Nichrome
* sproe print question evilone80 710 4 05/12/02 05:42 AM
by luvdemshrooms
* transferring mycelium wikedanjel 4,113 13 01/10/03 03:54 AM
by debianlinux
* Bulk spawn question
( 1 2 all )
Drink_Punk_Soda 5,441 22 10/17/02 02:23 AM
by XiC_clone

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
343 topic views. 34 members, 215 guests and 25 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.01 seconds on 14 queries.